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In order from best poker pony to worst(baring rule of funny)



1 Rarity she is the most collected and the best at reading ponies



2 Twilight second most composed and could probably use math to get a good sense of the odds on a given hoof



3 Applejack she is really good at reading others and wouldn't feel guilty about bluffing in a game were it is to be expected



4 A tie between Pinkie and Rainbow neither has allot of self control and are a little dense when it comes to reading other ponies



5 Poor poor Fluttershy she would fold a good hoof just to avoid hurting ant ponyelse's feelings other wise she'd be in the same bracket as Dash and Pinkie