By JOHN SEEWER, Associated Press

TOLEDO, Ohio (AP) — New tests Sunday showed toxins some still contaminating Lake Erie, leaving regional water supplies off limits and some 400,000 residents in parts of Ohio and Michigan headed into a third day of scrambling to stock up on drinking, cooking and bathing water.

Toledo Mayor D. Michael Collins said early Monday that tests done by the state and federal authorities and the city show a positive trend, but that additional testing will be done because some concerns remain.

The city council is to review the results at its regularly-scheduled meeting on Monday.

Ohio's fourth-largest city warned residents not to use city water early Saturday after tests at one treatment plant showed readings for microcystin above the standard for consumption, most likely from algae on the lake. The advisory affected residents in northwestern Ohio and southwestern Michigan. Ohio Gov. John Kasich declared a state of emergency.

With the warning, worried residents told not to drink, brush their teeth or wash dishes with the water descended on truckloads of bottled water delivered from across the state. The Ohio National Guard was using water purification systems to produce drinkable water.

Water distribution centers will reopen at 8 a.m. Monday.

Oliver Arnold, of Toledo, loaded up on bottled water Sunday so that he could give baths to his six children, including 4-month-old twins. "We're going through a lot. I know by tomorrow, we're going to be looking for water again," he said.

Some hospitals canceled elective surgeries and were sending surgical equipment that needed sterilized to facilities outside the water emergency, said Bryan Biggie, disaster coordinator for ProMedica hospitals in Toledo.

In southeastern Michigan, authorities were operating water stations Sunday for the 30,000 customers affected by the toxic contamination.

Drinking the water could cause vomiting, cramps and rashes. But no serious illnesses had been reported by late Sunday. Health officials advised children and those with weak immune systems to avoid showering or bathing in the water.

Amid the emergency, discussion began to center around how to stop the pollutants fouling the lake that supplies drinking water for 11 million people.

"People are finally waking up to the fact that this is not acceptable," Collins said.

The toxins that contaminated the region's drinking water supply didn't just suddenly appear.

Water plant operators along western Lake Erie have long been worried about this very scenario as a growing number of algae blooms have turned the water into a pea soup color in recent summers, leaving behind toxins that can sicken people and kill pets.

In fact, the problems on the shallowest of the five Great Lakes brought on by farm runoff and sludge from sewage treatment plants have been building for more than a decade.

The National Oceanic and Atmospheric Administration released a satellite image showing a small but concentrated algae bloom centered right where Toledo draws its water supply, said Jeff Reutter, head of the Ohio Sea Grant research lab.

The bloom was much smaller than in past years and isn't expected to peak until early September. But instead of being pushed out to the middle of the lake, winds and waves drove the algae toward the shore, he said.

"Weather conditions made it such that bloom was going right into the water intakes," said Reutter, who has been studying the lake since the 1970s, when it was severely polluted.

The amount of phosphorus going into the lake has risen every year since the mid-1990s. "We're right back to where we were in the '70s," Reutter said.

Almost a year ago, one township just east of Toledo told its 2,000 residents not to drink or use the water coming from their taps. That was believed to be the first time a city has banned residents from using the water because of toxins from algae in the lake.

Researchers largely blame the algae's resurgence on manure and chemical fertilizer from farms that wash into the lake along with sewage treatment plants. Leaky septic tanks and stormwater drains have contributed, too. Combined, they flush huge amounts of phosphorus into the lake.

Environmental groups and water researchers have been calling on Ohio and other states in the Great Lakes region to drastically reduce the amount of phosphorus flowing into the lake. Ohio lawmakers this past spring took a step toward tackling the algae problem when they enacted a law requiring most farmers to undergo training before they use commercial fertilizers on their fields.But they have stopped short of mandating restrictions on farmers.

The International Joint Commission, an advisory agency made up of Canadian and U.S. officials, said last year urgent steps are needed to reduce phosphorus applied to fields, suggesting among other things that states ban the spread of manure on frozen or snow-covered ground.

That report came after a state task force in Ohio called for a 40 percent reduction in all forms of phosphorus going into the lake.

Agriculture industry groups have been asking farmers for more than a year to reduce phosphorus runoff before government regulators step in and impose their own restrictions.

"We're clearly showing progress," Reutter said. "You have to decide for yourself whether you think it's fast enough."

In Michigan, Detroit's 4 million-user water system gets its water from Lake Huron and the Detroit River. In the face of the Toledo water crisis, Detroit officials plan to review their contamination procedures Monday, water department Deputy Director Darryl Latimer told The Detroit News. He said it was unlikely Detroit would face a problem like Toledo's.

"The system is tested every two weeks for blue-green algae," Latimer said. "We haven't seen the precursors for this type of toxin."