Three female high school runners in Connecticut have filed a federal lawsuit seeking to block transgender athletes from participating in girls’ sports.

The lawsuit, filed Wednesday by the conservative nonprofit group Alliance Defending Freedom, claims that the Connecticut Interscholastic Athletic Conference policy allows “biological boys” to beat them at track events and deprives them of possible scholarships.

“Girls deserve to compete on a level playing field,” ADF attorney Christiana Holcomb said in a statement. “Forcing them to compete against boys isn’t fair, shatters their dreams and destroys their athletic opportunities.”

Holcomb is representing high school track athletes Selina Soule, Chelsea Mitchell and Alanna Smith — all of whom allege they have suffered since CIAC first implemented its policy in 2017 allowing athletes with male anatomy to compete alongside girls, the Connecticut Post reports.

As a result, two athletes born “biologically male” have “taken 12 women’s state championship titles” previously held by nine different girls, Holcomb said.

“It is not fair for any boy to compete against girls,” Smith, the daughter of former Major League pitcher Lee Smith, said during Wednesday’s press conference. “That biological unfairness doesn’t go away because of what someone believes about gender identity.”

Smith insists her inclusion in the filing has nothing to with “lifestyle.”

“It’s simply about fairness of play,” she said.

The students are seeking a preliminary injunction to stop the policy. CIAC officials, meanwhile, said it would respond to the lawsuit after reviewing it, the Connecticut Post reports.

“The CIAC believes that its current policy is appropriate under both state and federal law, and it has been defending that policy in the complaint that was filed previously with the Office of Civil Rights,” the organization said in a statement.

Relatives of the girls and ADF officials also filed a Title IX complaint with the Department of Education in June.

The lawsuit cites two transgender high school athletes — seniors Terry Miller and Andraya Yearwood — who have competed directly against Soule, Mitchell and Smith.

Soule is a senior at Glastonbury High School, Mitchell is a senior at Canton High School and Smith is a sophomore at Danbury High School.

Miller, a senior at Bloomfield High School, and Yearwood, a senior at Cromwell High School, will be represented by the American Civil Liberties Union. Both have released statements indicating that they have no plans to stop competing.

“I will never stop being me!” Yearwood said. “I will never stop running! I hope that the next generations of trans youth doesn’t have to fight the fights that I have.”

Connecticut is one of 17 states that allowed transgender high school athletes to compete without restrictions in 2019, while 20 others either had no policies or handled each student on a case-by-case basic, according to Transathlete.com, a website that tracks high school policies for transgender athletes nationwide.