Stripe Inc. climbed closer to the top ranks of the highest-priced U.S. startups after a new fundraising round valued the financial-technology company at $35 billion.

Venture-capital firms Sequoia Capital, General Catalyst and Andreessen Horowitz were among the investors behind the $250 million investment, the company said Thursday. The $35 billion valuation, up about 50% from an early 2019 funding round, puts Stripe above Silicon Valley darlings Airbnb Inc. and Palantir Technologies Inc.

Stripe’s...