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Twenty-seven more women have accused Charlie Rose of sexual harassment, according to a report Thursday that says CBS was alerted to his alleged behavior as early as 1986 and as recently as April 2017.

The 76-year-old journalist was fired from CBS and PBS in November in light of sexual misconduct allegations from eight women.

But an additional 27 have now come forward — 14 CBS News employees and 13 others who worked with him elsewhere, according to the Washington Post, which conducted five months of interviews and spoke with more than 100 of his former colleagues.

The new allegations date back to 1976, when Rose allegedly exposed his penis to Joana Matthias, a former research assistant, and allegedly groped her breasts while working at NBC News’ Washington bureau.

“This other personality would come through, and the groping would happen,” Matthias, 63, told the newspaper.

An NBC News spokeswoman declined to comment.

Ten years later, then-22-year-old CBS news clerk Annmarie Parr reported to her boss inappropriate comments Rose made to her.

“Annmarie, do you like sex? Do you enjoy it? How often do you like to have sex?” Rose allegedly asked Parr in 1986 when he was filling in as an anchor on “CBS Morning News.”

Parr told her boss, a senior producer whom she declined to name, that she didn’t want to be alone with Rose.

But he laughed off Rose’s comments, telling her, “Fine, you don’t have to be alone with him anymore.”

CBS was sued that same year by seven women, including Beth Homan-Ross, who claimed the workplace on the network’s overnight broadcast “Nightwatch” was “offensive and hostile” to female workers.

Rose was a co-anchor for the show but was not named as a defendant in the suit — which was settled under confidential terms in 1987.

Homan-Ross said Rose frequently made sexual comments about her breasts and buttocks while she was working as his assistant producer.

Her work required her to travel to Rose’s home. Sometimes, she said, he would open the door naked, holding a towel, and more than once he invited her into his bathroom while he showered.

“It was a sexual land mine everywhere you stepped,” said Homan-Ross, now 61.

Sophie Gayter, now 27, worked with Rose at CBS’ “60 Minutes” and claims he touched her buttocks in 2013 as they walked down a hallway to a recording studio.

She and other women said they feared reporting his behavior to higher-ups, believing male employees’ careers were prioritized over the women’s.

“I had been there long enough to know that it was just the way things went,” said Gayter. “People said what they wanted to you, people did what they wanted to you.”

Managers at CBS were alerted two other times to Rose’s alleged creepy behavior — in late 2011 and early 2017, the Washington Post found.

A “CBS This Morning” employee reported to her boss Chris Licht that Rose forcibly kissed her at a holiday party in Manhattan — but begged Licht not to tell human resources, according to the report.

Licht, who was an executive producer on the show before he was promoted, confirmed to the newspaper that he was told about the incident and abided by the woman’s wishes. He said he spoke with Rose about it.

Meanwhile, Rose allegedly began targeting Brooks Harris, then 24, last year when she was briefly assigned to work in the studio for “CBS This Morning.”

Rose started taking Harris out for lunch to expensive restaurants, plying her with wine and offering job opportunities on “60 Minutes” and his PBS show “Charlie Rose.”

“I was nobody, and he picked me out of a crowd of employees,” Harris recalled.

One of her colleagues, executive assistant Chelsea Wei, said she became concerned over the attention Rose was giving Harris, and told “CBS This Morning” executive producer Ryan Kadro.

“I’m telling you in case you have a lawsuit on your hands,” Wei told Kadro, the report said.

Kadro acknowledged to the Washington Post that Wei spoke to him about Rose but said she “did not tell me about inappropriate behavior by Charlie Rose towards Ms. Harris at any time … Regarding your question about a ‘lawsuit’ — I don’t believe she used that word.”

Harris said a drunken Rose invited her up to his apartment after a staff gathering last July, insisting they watch footage of his “60 Minutes” interviews of former US presidents.

Uncomfortable, Harris — who by then had landed a job on the “Charlie Rose” show — said she made her excuses and bolted from the apartment.

Four months later, she and others lost their jobs when PBS yanked “Charlie Rose” off the air — as the original sexual harassment allegations came to light.

Rose denied the fresh wave of allegations.

“Your story is unfair and inaccurate,” he said in an email to the Washington Post.

CBS has said it had no human resources complaints about Rose.

“Since we terminated Charlie Rose, we’ve worked to strengthen existing systems to ensure a safe environment where everyone can do their best work,” the network said in a statement. “Some of the actions we have taken have been reported publicly, some have not. We offer employees discretion and fairness, and we take swift action when we learn of unacceptable behavior.

“That said, we cannot corroborate or confirm many of the situations described. We continue to look for ways to improve our workplace and this period of reflection and action has been important to all of us. We are not done with this process.”