Synthetic Biology Tutorial









Lectures

If you'd like a more advanced series of topics, you might check out my video lectures: http://www.youtube.com/channel/UCSVDJJWZ77tOzwl4L9nyg2w/videos?view=1&sort=da&flow=list

They are listed in order here: Synthetic Biology Trails

The Basics

Think the "Central Dogma" involves a kennel of chihuahuas? You need to start here with the basics.

Bare Bones Biochemistry



Basic Molecular Biology



Practical DNA



What is PCR?





Introduction to Biobricks and Basic Cloning

This tutorial is best used in conjunction with the sequence editor ApE. Read the section about ApE for a description of how to install it.

Installing ApE



Overview of Cloning



Introduction to Biobricks



Introduction to Refactoring





Technical Design

Basic Oligo Design (old)



Special Case: Single Internal Restriction Sites (old)



Construction of short parts



Golden Gate Cloning



Design of composite parts



Keeping track of construction files



Sequencing Analysis





Advanced Technical Design

Working with Translational Fusions



Circularly Permuted Proteins



The "what did they make" game





Implementation

Implementing the Basic Construction File





Introduction to Structural Biology and Part Engineering

DeepView Basics



Tour of Structural Biology





Things you should know

Common Pitfalls and Tips



Origins of replication



The Critical Path









Retired Sections

To Be Added

If you have any comments or want to report a potential error in the tutorial, please email me (Chris Anderson) at JCAnderson2167-at-gmail.com



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