FILE - In this Wednesday, Aug. 8, 2018, file photo, U.S. Sen. Elizabeth Warren, D-Mass., speaks during a town hall-style gathering in Woburn, Ma. A DNA analysis done on Sen. Warren provides strong evidence she has Native American heritage. She provided her test results to The Boston Globe for a story published Monday. (AP Photo/Charles Krupa, File)

FILE - In this Wednesday, Aug. 8, 2018, file photo, U.S. Sen. Elizabeth Warren, D-Mass., speaks during a town hall-style gathering in Woburn, Ma. A DNA analysis done on Sen. Warren provides strong evidence she has Native American heritage. She provided her test results to The Boston Globe for a story published Monday. (AP Photo/Charles Krupa, File)

BOSTON (AP) — Sen. Elizabeth Warren on Monday released the results of a DNA analysis that she said indicated that she has some Native American heritage, a rebuttal to President Donald Trump, who has long mocked her ancestral claims and repeatedly referred to her as “Pocahontas.”

The Massachusetts Democrat and potential 2020 presidential contender challenged Trump to make good on his pledge to donate $1 million to charity if she provided proof of Native American heritage, a moment that was caught on video. Trump falsely denied ever making the offer and later said he would donate the money only if he can personally administer the genetic test.

ADVERTISEMENT

The analysis was done by Stanford University professor Carlos D. Bustamante, a prominent expert in the field. He concluded that the great majority of Warren’s ancestry is European but added that the results “strongly support” the existence of a Native American ancestor.

In his report , Bustamante said he analyzed Warren’s sample without knowing the identity of the donor. He concluded that Warren has a pure Native American ancestor who probably lived six to 10 generations ago, and that it was impossible to determine the individual’s tribal connection.

Warren, who has said her Native American roots were part of “family lore,” also released a video produced by her Senate re-election campaign. In it, she said: “The president likes to call my mom a liar. What do the facts say?”

Bustamante replied: “The facts suggest that you absolutely have Native American ancestry in your pedigree.”

The analysis is not the first evidence of Warren’s heritage. An 1894 document previously unearthed by the New England Genealogical Society suggested Warren’s great-great-great-grandmother, O.C. Sarah Smith, was at least partially Native American, making the senator as much as 1/32nd Native American.

The genealogy group has said it has no conclusive evidence of her ancestry, and a spokesman said Monday it would not comment on the genetic findings.

If Warren’s ancestor were six generations removed, she would be 1/64th Native American. But if her ancestor had been as much as 10 generations removed, that would make the individual a great-great-great-great-great-great-great-great grandparent and render Warren only 1/1,024th Native American, according to Blaine Bettinger, a genealogist and author who specializes in DNA evidence.

Such a finding could potentially further excite Warren’s critics instead of placating them.

Cherokee Nation Secretary of State Chuck Hoskin Jr. said DNA tests are useless to determine tribal citizenship and don’t distinguish whether a person’s ancestors were indigenous to North or South America.

“Using a DNA test to lay claim to any connection to the Cherokee Nation or any tribal nation, even vaguely, is inappropriate and wrong,” he said Monday. “Senator Warren is undermining tribal interests with her continued claims of tribal heritage.”

Warren acknowledged in a tweet that DNA and family history have nothing to do with tribal affiliation or citizenship, which is determined by tribal nations. “I respect the distinction, & don’t list myself as Native in the Senate,” she said.

Warren’s effort to address questions about her ancestry and the release of the video are her latest moves telegraphing a likely presidential run in 2020. During the summer, she also released a decade’s worth of tax returns, drawing a contrast with Trump’s unwillingness to release his own tax documents.

The moves seem to anticipate the type of criticism she might face against opponents in a Democratic primary or in a possible general election matchup against Trump.

“She is most clearly doing the things you do if you’re running for president,” said Steve Elmendorf, a Democratic strategist and veteran of presidential campaigns.

Some Democrats were critical of the timing of Warren’s announcement.

Jim Messina, who served as President Barack Obama’s 2012 campaign manager and as a White House deputy chief of staff, said on Twitter: “Argue the substance all you want, but why 22 days before a crucial election where we MUST win House and Senate to save America, why did @SenWarren have to do her announcement now? Why can’t Dems ever stay focused?”

During a recent town hall-style meeting in Holyoke, Massachusetts, Warren said she planned to “take a hard look at running for president,” after next month’s election.

Earlier this year, the senator released personnel files seeking to dispute critics who have alleged that the former Harvard Law School professor advanced her law career with a narrative that she is a descendant of Cherokee and Delaware tribes. Warren has denied using her Native American heritage to gain any advantage.

In an email Monday to supporters, Warren said she “never expected the president of the United States to use my family’s story as a racist political joke against Native American history, culture, and people — over, and over, and over.”

In a tweet directed at Trump, Warren said: “Remember saying on (July 5) that you’d give $1M to a charity of my choice if my DNA showed Native American ancestry?” She went on to request that the president send a check to National Indigenous Women’s Resource Center.

At a summer rally in Montana, the president declared that he would give a million dollars to charity, “paid for by Trump,” if Warren takes the test “and it shows you’re an Indian.”

But when asked by reporters Monday, Trump said, “I didn’t say that.”

Hours later, when asked about the donation during an appearance in Georgia, Trump said he would “only do it if I can test her personally.” He added, “That will not be something I enjoy doing, either.”

Warren responded with a series of tweets, suggesting that Trump’s comments are “creepy physical threats,” the kind he makes toward women who scare him. Warren tweeted Trump is a “cowardly elitist” and she “won’t sit quietly for Trump’s racism” so she took the test.

Warren, who grew up in Norman, Oklahoma, said her mother and father were forced to elope because of her mother’s heritage.

She faces Republican Geoff Diehl, who co-chaired Trump’s Massachusetts presidential campaign, in November. Diehl said it’s up to voters to decide what they think of Warren’s DNA analysis.

“We’ve never made it an issue with this campaign. I think the fact is it’s an issue that’s been attached to her since 2012,” he said.

The DNA analysis was first reported by The Boston Globe.

___

This story has been updated to correct an earlier reference to the percentage of Native American ancestry had the ancestor lived 10 generations ago to 1/1,024th, instead of 1/512th.

___

Associated Press writers Eileen Putman and Ken Thomas in Washington and Steve LeBlanc in Boston contributed to this report.