Tennessee Gov. Bill Lee speaks during a Gospel Music Association ceremony in Nashville. He plans to sign an adoption bill that the LGBTQ community and many businesses say is discriminatory.

A total of 142 businesses signed a letter Wednesday announcing their opposition to Tennessee's recent slew of anti-LGBTQ laws, which has been dubbed the slate of hate by activist groups.

The collective, known as the Tennessee Businesses Against Discrimination, included major companies such as Amazon, Nike, Dell, Lyft, Marriott and American Airlines, as well as more than 100 small businesses in Tennessee.

The letter specifically targets the passage of HB 836, which allows taxpayer-funded foster care and adoption agencies to discriminate against LGBTQ people. It stated that enacting further anti-LGBTQ legislation would harm Tennesseans and "create unnecessary hurdles to economic competitiveness."

"Policies that signal that the state is not welcoming to everyone put our collective economic success at risk," the letter said. "It is both a business imperative and core to our corporate values that our customers, our employees and their families, and our potential employees feel fully included in the prosperity of our state."

Tennessee received more than $22 billion in tourist spending in 2018, according to Nashville Business Journal. Nashville has become a recent hub for tech-sector expansion, with a high quality-of-life ranking. Amazon is in the process of adding 5,000 jobs as part of an operations hub in the city — Tennessee's largest jobs deal ever.

FedEx, the largest company headquartered in Tennessee, did not sign the letter. FedEx did not immediately respond to a request for comment, but according to LGBTQ rights group GLAAD, which asked FedEx to sign the letter, the delivery services company indicated in writing that while it receives many requests to join advocacy efforts, it prefers to demonstrate corporate values through civic and charitable efforts.

GLAAD also told CNBC via email, "As one of the largest companies headquartered in Tennessee, FedEx has an obligation to its LGBTQ employees in Tennessee and its customers nationwide to use its public voice and brand power to help stop these dangerous anti-LGBTQ bills. With more than three dozen large corporations speaking out against the slate of legislation today, FedEx's refusal to address is notable and out of step with other companies doing business in Tennessee."

The letter, organized by the Nashville LGBT Chamber of Commerce, GLAAD, Human Rights Campaign, and Freedom for All Americans, is the latest in a series of businesses speaking out against Tennessee's discriminatory laws. In April, when HB 836 was first introduced, 13 large companies signed a similar letter, including Nike, Hilton, Lyft and Marriott, but Gov. Lee signed the bill into law on Jan. 24.

"I think equality is important and protection of rights is important and the rights of religious liberty are important," Gov. Lee said in a recent interview on Nashville's News Channel 5. "And that bill was centered around protection of religious liberty, and that's why I signed it."

Gov. Lee's office did not immediately respond to a request for comment, though it previously has told CNBC that critics had mischaracterized the bill.