Trace Lysette (pictured), who plays yoga instructor Shea in the show, claims Tambor pressed up against her in a sexual way during filming and repeatedly made lewd sexually suggestive remarks

Jeffrey Tambor has been accused of sexual assault by a second transgender person - his fellow Transparent actor Trace Lysette.

Lysette, who plays yoga instructor Shea in the show, claims Tambor pressed up against her in a sexual way during filming and repeatedly made lewd sexually suggestive remarks.

She said she felt his penis against her as he made 'quick, discreet thrusts' at her body after standing on her feet.

It comes after it was announced that Award-winning Amazon series Transparent may continue without its main character after star Jeffrey Tambor was accused of sexual harassment by another transgender woman last week.

His assistant, Van Barnes, said on November 8 that Tambor, 73, was accused by Barnes in a private Facebook post of repeatedly propositioning her, groping her and making lewd comments, Deadline previously reported.

Lysette said she thought it would be best to 'remove the problem and let the show go on'.

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Trouble: Jeffrey Tambor, 73, may be dropped from his starring role in Amazon's Transparent. In the celebrated show he plays Maura, a transgender mom who transitions late in life

Lysette (left) said she felt his penis against her as he made 'quick, discreet thrusts' at her body after standing on her feet

Accused: He was accused of groping and lewd remarks by transgender ex-assistant Van Barnes (left and right). The show's writers are now working on the fifth season of the series

Amazon said the allegations will be added to its ongoing investigation into Tambor.

But Deadline reports that no official complaint has been made to Amazon by Lysette.

Lysette claims that, when she appeared for a scene in a lingerie top and shorts, Tambor said to her: 'My God, Trace. I want to attack you sexually.'

The remark was apparently 'laughed off' because it was 'so absurd,' according to the Hollywood Reporter.

But minutes later, Lysette alleges that Tambor - while dressed as transgender woman Maura - put his feet on top of hers and 'began quick, discreet thrusts back and forth against my body'.

Cast: The show has a sizeable cast (pictured) but could struggle to go on without Tambor (bottom-right), whose character the show is named after

She added: 'I felt his penis on my hip through his thin pajamas.'

The incident might have looked like he was giving her a hug, she said, because it was so discreet.

She said she pushed him off and rolled her eyes.

Although the show's concept - and its title - are based on Tambor's transgender mom, insiders said that the claims made by Van Barnes, who is also transgender, may have made his position untenable.

That means they're now working out how the fifth season can progress without Tambor's involvement, according to Deadline.

But Tambor has denied he is a sexual predator.

In a statement, he explained: 'For the past four years, I've had the huge privilege — and huge responsibility — of playing Maura Pfefferman, a transgender woman, in a show that I know has had an enormous, positive impact on a community that has been too long dismissed and misunderstood.

'Now I find myself accused of behavior that any civilised person would condemn unreservedly. I know I haven't always been the easiest person to work with. I can be volatile and ill-tempered, and too often I express my opinions harshly and without tact.

'But I have never been a predator — ever. I am deeply sorry if any action of mine was ever misinterpreted by anyone as being sexually aggressive or if I ever offended or hurt anyone. But the fact is, for all my flaws, I am not a predator and the idea that someone might see me in that way is more distressing than I can express.'

Van Barnes said he threatened to sue her if she spoke out about him.

Tambor was not named in the post, but later issued a statement in which he identified himself as the target of the message and denied all the claims.

Amazon Studios launched an investigation into the claims on November 8, and has yet to make any announcement about the show's future.

But it is in a difficult position, as Transparent was its first breakout success, and attracts a socially conscious audience.

That the claims made against Tambor came from a transgender woman only makes the situation worse for them.

And while Netflix is in a similar situation with House of Cards after claims of abuse emerged about star Kevin Spacey, it doesn't have the advantage of as much flexibility.

After all, Transparent's entire title is based around the show's transgender matriarch.

The show's creator, Jill Soloway, who identifies as being gender non-binary, said the show's team would comply with Amazon's investigation.

Soloway added in a statement: 'Anything that would diminish the level of respect, safety and inclusion so fundamental to our workplace is completely antithetical to our principles.'

In his own statement, Tambor said: 'I am aware that a former disgruntled assistant of mine has made a private post implying that I had acted in an improper manner toward her.

'I adamantly and vehemently reject and deny any and all implication and allegation that I have ever engaged in any improper behavior toward this person or any other person I have ever worked with.

'I am appalled and distressed by this baseless allegation.'

In October Amazon Studios boss Roy Price resigned over sexual harassment claims from July 2015 made by producer Isa Hackett.

Hackett told The Hollywood Reporter that after being introduced to her, Price allegedly suggested they 'have an affair' to help promote her series and bragged about his sexual prowess, such as telling her 'You will love my d**k'.

Just hours after Hackett's story was published, Amazon suspended Price. He resigned the following day.