1 Fu, Q. et al. Genome sequence of a 45,000-year-old modern human from western Siberia. Nature 514, 445–449 (2014)

2 Seguin-Orlando, A. et al. Genomic structure in Europeans dating back at least 36,200 years. Science 346, 1113–1118 (2014)

3 Rasmussen, M. et al. An Aboriginal Australian genome reveals separate human dispersals into Asia. Science 334, 94–98 (2011)

4 Raghavan, M. et al. Upper Palaeolithic Siberian genome reveals dual ancestry of Native Americans. Nature 505, 87–91 (2014)

5 Raghavan, M. et al. The genetic prehistory of the New World Arctic. Science 345, 1255832 (2014)

6 Rasmussen, M. et al. The genome of a Late Pleistocene human from a Clovis burial site in western Montana. Nature 506, 225–229 (2014)

7 Bramanti, B. et al. Genetic discontinuity between local hunter-gatherers and Central Europe’s first farmers. Science 326, 137–140 (2009)

8 Malmström, H. et al. Ancient DNA reveals lack of continuity between Neolithic hunter-gatherers and contemporary Scandinavians. Curr. Biol. 19, 1758–1762 (2009)

9 Skoglund, P. et al. Origins and genetic legacy of Neolithic farmers and hunter-gatherers in Europe. Science 336, 466–469 (2012)

10 Lazaridis, I. et al. Ancient human genomes suggest three ancestral populations for present-day Europeans. Nature 513, 409–413 (2014)

11 Haak, W. et al. Ancient DNA from European early Neolithic farmers reveals their Near Eastern affinities. PLoS Biol. 8, e1000536 (2010)

12 Gamba, C. et al. Genome flux and stasis in a five millennium transect of European prehistory. Nature Commun. 5, 5257 (2014)

13 Kristiansen, K. in The World System and the Earth System. Global Socioenvironmental Change and Sustainability Since the Neolithic (eds Hornborg, B. & Crumley, C.) (Left Coast Press, 2007)

14 Shishlina, N. Reconstruction of the Bronze Age of the Caspian Steppes. Life Styles and Life Ways of Pastoral Nomads. Vol. 1876 (Archaeopress, 2008)

15 Anthony, D. The Horse, The Wheel and Language. How Bronze-Age Riders from the Eurasian Steppes Shaped the Modern World (Princeton Univ. Press, 2007)

16 Harrison, R. & Heyd, V. The Transformation of Europe in the third millennium BC: the example of ‘Le Petit-Chasseur I + III’ (Sion, Valais, Switzerland). Praehistorische Zeitschrift. 82, 129–214 (2007)

17 Vandkilde, H. Culture and Change in the Central European Prehistory, 6th to 1st millennium BC (Aarhus Univ. Press, 2007)

18 Kristiansen, K. & Larsson, T. The Rise of Bronze Age Society. Travels, Transmissions and Transformations (Cambridge Univ. Press, 2005)

19 Hanks, B. K., Epimakhov, A. V. & Renfrew, A. C. Towards a refined chronology for the Bronze Age of the southern Urals, Russia. Antiquity 81, 353–367 (2007)

20 Kuznetsov, P. F. The emergence of Bronze Age chariots in Eastern Europe. Antiquity 80, 638–645 (2006)

21 Koryakova, L. & Epimakhov, A. V. The Urals and Western Siberia in the Bronze and Iron Ages (Cambridge Univ. Press, 2007)

22 Rasmussen, M. et al. Ancient human genome sequence of an extinct Palaeo-Eskimo. Nature 463, 757–762 (2010)

23 Carpenter, M. L. et al. Pulling out the 1%: whole-genome capture for the targeted enrichment of ancient DNA sequencing libraries. Am. J. Hum. Genet. 93, 852–864 (2013)

24 Barros Damgaard, P. d. et al. Improving access to endogenous DNA in ancient bones and teeth. Preprint at bioRxivhttp://dx.doi.org/10.1101/014985 (2015)

25 Adler, C. J., Haak, W., Donlon, D., Cooper, A. & The Genographic Consortium Survival and recovery of DNA from ancient teeth and bones. J. Archaeol. Sci. 38, 956–964 (2011)

26 Orlando, L. et al. True single-molecule DNA sequencing of a Pleistocene horse bone. Genome Res. 21, 1705–1719 (2011)

27 Olalde, I. & Lalueza-Fox, C. Modern humans’ paleogenomics and the new evidences on the European prehistory. Science and Technology of Archaeological Research 1, http://dx.doi.org/10.1179/2054892315Y.0000000002 (2015)

28 Grigoriev, S. Ancient Indo-Europeans (Charoid, 2002)

29 Bendezu-Sarmiento, J. De l’Âge du Bronze et lÂge du Fer au Kazakkstan, gestes funéraires et paramètres biologiques. Identités culturelles des population Andronovo et Saka (De Boccard, 2007)

30 Kozintsev, A. G., Gromov, A. V. & Moiseyev, V. G. Collateral relatives of American Indians among the Bronze Age populations of Siberia? Am. J. Phys. Anthropol. 108, 193–204 (1999)

31 Kristiansen, K. in Becoming European. The transformation of third millennium Northern and Western Europe (eds Prescott, C. & Glørstad, H.) (Oxbow Books, 2012)

32 Haak, W. et al. Massive migration from the steppe was a source for Indo-European languages in Europe. Nature http://dx.doi.org/10.1038/nature14317 (this issue)

33 Olalde, I. et al. Derived immune and ancestral pigmentation alleles in a 7,000-year-old Mesolithic European. Nature 507, 225–228 (2014)

34 Itan, Y., Powell, A., Beaumont, M. A., Burger, J. & Thomas, M. G. The origins of lactase persistence in Europe. PLoS Computational Biol. 5, e1000491 (2009)

35 Mallory, J. In Search of the Indo-Europeans. Language, Archaeology and Myth (Thames & Hudson, 1987)

36 Renfrew, A. C. Archaeology and Language. The Puzzle of Indo-European Origins (Penguin, 1987)

37 Mallory, J. & Mair, V. The Tarim Mummies. Ancient China and the Mystery of the Earliest People from the West (Thames & Hudson, 2000)

38 Keyser, C. et al. Ancient DNA provides new insights into the history of south Siberian Kurgan people. Hum. Genet. 126, 395–410 (2009)

39 Meyer, M. & Kircher, M. Illumina sequencing library preparation for highly multiplexed target capture and sequencing. Cold Spring Harb. Protocols (2010)

40 Orlando, L. et al. Recalibrating Equus evolution using the genome sequence of an early Middle Pleistocene horse. Nature 499, 74–78 (2013)

41 Malaspinas, A.-S. et al. Two ancient human genomes reveal Polynesian ancestry among the indigenous Botocudos of Brazil. Curr. Biol. 24, R1035–R1037 (2014)

42 Willerslev, E. & Cooper, A. Ancient DNA. Proc. Royal Soc. B 272, 3–16 (2005)

43 Briggs, A. W. et al. Patterns of damage in genomic DNA sequences from a Neandertal. Proc. Natl Acad. Sci. USA 104, 14616–14621 (2007)

44 Li, H. & Durbin, R. Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics 25, 1754–1760 (2009)

45 Schubert, M. et al. Improving ancient DNA read mapping against modern reference genomes. BMC Genomics 13, 178 (2012)

46 Li, H. et al. The Sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009)

47 Quinlan, A. R. & Hall, I. M. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics 26, 841–842 (2010)

48 Jónsson, H., Ginolhac, A., Schubert, M., Johnson, P. & Orlando, L. mapDamage2.0: fast approximate Bayesian estimates of ancient DNA damage parameters. Bioinformatics (2013)

49 Fu, Q. et al. DNA analysis of an early modern human from Tianyuan Cave, China. Proc. Natl Acad. Sci. USA 110, 2223–2227 (2013)

50 Korneliussen, T. S., Albrechtsen, A. & Nielsen, R. ANGSD: analysis of next generation sequencing data. BMC Bioinformatics 15, (2014)

51 Patterson, N., Price, A. L. & Reich, D. Population structure and Eigenanalysis. PLoS Genet. 2, e190 (2006)

52 Alexander, D. H., Novembre, J. & Lange, K. Fast model-based estimation of ancestry in unrelated individuals. Genome Res. 19, 1655–1664 (2009)

53 Patterson, N. et al. Ancient admixture in human history. Genetics 192, 1065–1093 (2012)

54 Weir, B. S. & Hill, W. Estimating F-statistics. Annu. Rev. Genet. 36, 721–750 (2002)

55 Nyström, V. et al. Microsatellite genotyping reveals end-Pleistocene decline in mammoth autosomal genetic variation. Mol. Ecol. 21, 3391–3402 (2012)