Donald Trump again attacked House Speaker Paul Ryan (R-Wis.) on Tuesday, calling him a “very weak and ineffective leader” and criticizing the GOP for its lack of loyalty, after Ryan told House Republicans he would no longer defend his party’s presidential nominee.

Our very weak and ineffective leader, Paul Ryan, had a bad conference call where his members went wild at his disloyalty. — Donald J. Trump (@realDonaldTrump) October 11, 2016

Despite winning the second debate in a landslide (every poll), it is hard to do well when Paul Ryan and others give zero support! — Donald J. Trump (@realDonaldTrump) October 11, 2016

Trump tried to spin the lack of support from his own party as a good thing on Tuesday, saying it would allow him to campaign the way he wanted to.

It is so nice that the shackles have been taken off me and I can now fight for America the way I want to. — Donald J. Trump (@realDonaldTrump) October 11, 2016

And then he threw some shade at the GOP, and continued rolling out tweets through the afternoon.

With the exception of cheating Bernie out of the nom the Dems have always proven to be far more loyal to each other than the Republicans! — Donald J. Trump (@realDonaldTrump) October 11, 2016

Disloyal R's are far more difficult than Crooked Hillary. They come at you from all sides. They don’t know how to win - I will teach them! — Donald J. Trump (@realDonaldTrump) October 11, 2016

The very foul mouthed Sen. John McCain begged for my support during his primary (I gave, he won), then dropped me over locker room remarks! — Donald J. Trump (@realDonaldTrump) October 11, 2016

After a 2005 video emerged featuring Trump making vulgar comments about women, Ryan told House Republicans on Monday that he would no longer campaign with Trump, but wouldn’t withdraw his endorsement. He also told members that he would instead focus on preserving a Republican majority in Congress and they should do what is needed to best defend their seats. Over the weekend, a number of Republicans denounced Trump and some even called for him to remove his name from the GOP ticket.

Ryan reportedly will not respond to Trump’s tweets.

@DanaBashCNN reports @SpeakerRyan will not respond to @realDonaldTrump tweets. Spox: "not going to get into a back and forth with him" #cnn — Matt Hoye (@mattyhoyeCNN) October 11, 2016

Trump and Ryan were also scheduled to appear at the same event in Wisconsin on Saturday, but Trump did not appear after The Washington Post published a report with the video on Friday.

Indiana Gov. Mike Pence, the GOP nominee for vice president, told NBC News on Tuesday that he disagrees with Ryan’s “focus in this campaign.” Just last month, Pence visited Capitol Hill, where he praised Ryan and talked about how the speaker’s vision for America aligns with Trump’s.

“I truly do believe Republican leaders should join millions of Americans and support the Republican nominee,” Pence said Tuesday.

Not all of the Republicans on Monday’s conference call seemed so concerned about Trump’s comments. Rep. Dana Rohrabacher (R-Calif.) said he was willing to sacrifice his seat to block Hillary Clinton from getting to the White House, and Rep. Trent Franks (R-Ariz.) said the comments weren’t as important as appointing a conservative Supreme Court justice.

Trump, who also attacked Ryan on Monday, has long had public tension with the House speaker. Ryan said he wasn’t ready to endorse Trump in May, Trump in turn declined to endorse Ryan’s re-election. The two did eventually endorse each other.

This post has been updated with additional Trump tweets and Mike Pence’s reaction.

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Editor’s note: Donald Trump regularly incites political violence and is a serial liar, rampant xenophobe, racist, misogynist and birther who has repeatedly pledged to ban all Muslims — 1.6 billion members of an entire religion — from entering the U.S.