66 Progress (66P) Launch: All 66P systems are performing nominally. One rendezvous maneuver, DV3, was performed nominally earlier today. Checkouts of the Progress TORU system and the Progress TV system 9 have been completed with nominal results. Docking is scheduled for Friday, February 24th at 2:35 am CST.

SpaceX (SpX)-10 Capture: After yesterday’s aborted rendezvous, a successful re-rendezvous and capture was performed this morning. The crew monitored the SpaceX-10 Dragon approach from the Cupola Robotic Workstation (RWS) Dragon vehicle was captured using the Space Station Remote Manipulator System (SSRMS) at 04:44 am CST today. Robotics Ground Controllers then used the SSRMS to maneuver the Dragon to and install it on the Node-2 Nadir Active Common Berthing Mechanism (CBM). This afternoon, the crew performed vestibule outfitting, CBM Control Panel Assembly (CPA) removal, and ingressed the vehicle. After ingress, the crew removed the Center Stack then retrieved and unpacked the Dragon Cargo Transfer Bag (CTB) and Double Coldbags. The Space Automated Bioproduct Laboratory (SABL) CO2 Incubator Installation and the Merlin Hardware Setup were done in preparation for Microgravity Extended Stem Cells (MESC) operations scheduled for early tomorrow. Installation of TangoLab Card Cube Replace, NanoRacks Mod9 Ops, Low Temperature (LT) Protein Crystal Growth (PCG) Sample Preparation and NanoRacks Module-71 were also performed.

NanoRacks Module 9: The crew retrieved the NanoRacks Module-9 from SpX-10 and activated five tubes of Student Spaceflight Experiments Program (SSEP)/National Center for Earth and Space Science Education (NCESSE) experiments. Supporting various schools and student designed experiments studying a range of objectives, the SSEP lets students design experiments that address real challenges of living and working in space. The program also is a key initiative for U.S. science, technology, engineering and math (STEM) education, educating and inspiring the next generation of scientists and engineers to work on the space program. Additionally, findings from student experiments such as those on bacteria growth, cell biology, food production and preservation, water quality, and seed and plant studies contribute to future experiments to benefit the space program. The following experiments were activated today:

The Effects of Microgravity on Muscle Tissue Regeneration (McNair Academic High School, Grade 12, Jersey City, NJ)

Shewanella oneidensis and Iron Ions in Microgravity (Bullis School, Grade 10, Potomac, MD)

The Effect of Microgravity on Preservation of Spam Using Lemon Juice (Lincoln Middle School, Grade 8, Santa Monica, CA)

Kidney Stone Conundrum (The Academy at Nola Dunn, Grade 5, Burleson, TX)

Microgravity’s Effects on the Turbidity of a Fluid Mixture of Cornstarch and Water (W.J. Keenan High School, Grade 9, Columbia, SC)

JAXA Low Temperature Protein Crystal Growth (LT PCG): The crew removed the samples from the Freezer-Refrigerator Of STirling cycle (FROST) then initiated the crystal growth. The crew activated the three different crystal sample containers then returned them to the FROST to maintain the optimal growth temperature of 4 degrees Celsius. The goal of the JAXA LT PCG experiment is to make high quality protein crystals under microgravity environmental conditions. Once growth is completed, the LT PCG experiment will be returned on SpX-10 for ground team evaluation and analysis.

NanoRacks Module-71/Platform 1 Installation: The crew installed NanoRacks Module-71 into Platform 1. Module-71 contains two plant growth experiments that will run onboard the ISS in the Platform-1 facility through the end of the SpX-10 mission. Module-71 contains two plant growth experiments, NanoRacks-Valley Christian High School-BAM-FX– Enhanced Broccoli Seed Germination, Plant Growth and Zinc Biofortification on Orbit (NanoRacks-VCHS-BAM-FX) and NanoRacks-Edith Stein School-Vegetative Propagation of Plants in Orbit (V3PO), that aim to investigate the capability for production of large quantities of high quality vegetables on a space station. Results from the experiment will aid researchers in developing methods to provide astronauts a continuous supply of fresh vegetables on long missions. NanoRacks-VCHS-BAM-FX studies the growth of broccoli plants fortified with zinc, looking at seed germination and growth in microgravity, determining whether a bioavailable mineral nutrient formula will help plant growth. The team responsible for V3PO is interested in vegetative plant propagation versus seeded propagation. In vegetative growth (cuttings) the desired characteristics are passed to subsequent generations of plants and ensures a uniform crop even when a few viable seeds are produced.

Payload Card-X: The crew retrieved the inserts from SpX-10 and installed them into the TangoLab facility. The inserts on Payload Card-X cover a wide spectrum of studies including:

Flatworms2.0: Follow up study to original flatworm regeneration study on SpX-5. Will include video and feeding capability.

Smooth Muscle Cell Stimulus Response: Study of — cell line after cultured in microgravity for 4 days will be hit with reagent then acetone to video response.

Tissue Mimetic: Looking at diffusion rates of different molecular compounds in tissue proxy. Precursor to advanced tissue cultures.

Methanogen Growth Characterization: Comparison of growth curves between Earth and microgravity of Methanogens.

Arabidopsis Study (Education): Arabidopsis study will be networked with real time control units in classrooms in San Francisco area of California. Precursor to larger undertaking with more schools on SpX-11 mission.

Pharmaceutical Compound Generation: Madagascar Periwinkle and Valeriana plants will be germinated on orbit to looking at mutation and generation of compounds used in synthesis of chemotherapies.

Today’s Planned Activities

All activities are on schedule unless otherwise noted.

Crew Command Panel (CCP) Cable Route and Checkout

Closing Shutters on windows 6, 8, 12, 13, 14.

Photo/TV Ghost Camera in Cuopla on SSC power recording

Inventory Management System (IMS) conference

Robotic Workstation (RWS) High Definition (HD) Monitor Downlink

Robotics Workstation (RWS) Dragon Configuration

CARDIOVECTOR. Experiment Ops

Dragon R Bar Approach

DUBRAVA. Video of VSS P/L screen

CARDIOVECTOR. Experiment Ops.

BIOPLENKA. Installation of Cryogem-03

Space Station Remote Manipulator System (SSRMS) Dragon Capture

BIOPLENKA. Removal of ТБУ-В No.04 Thermostat

Remote Workstation Monitor HD Video Downlink Deactivation RWS.

Ghost camera teardown in Cupola

PRODUTSENT. ТБУ-В No.2 Incubator Setup

Photo TV High Definition (HD) Cygnus Video Setup

MICROVIR. Glovebox-S Setup

Air Heater Fan (БВН) Screen Cleaning in Soyuz 732

NanoRacks Module-9 OBT

Vacuum Cleaning area behind panels 405, 406 and MRM1 Interior

Video recording

Crew Command Panel (CCP) Cable Deroute

Water Recovery System (WRS) Waste Water (WW) Tank Drain Initiation

Extravehicular Mobility Unit (EMU) Long Life Battery (LLB) Terminate

ESA Weekly crew conference

Extravehicular Mobility Unit (EMU) Long Life Battery (LLB) Initiate

Water Recovery System (WRS) Waste Water (WW) Tank Drain Termination

Node 2 to Dragon Pressurization and Leak Check Equipment Pregather

ISS HAM Radio Power Down

Node 2 to Dragon Pressurization and Leak Check

Dragon Vestibule Outfitting

Crew Quarters (CQ) Door Closing

Node 2 Nadir Common Berthing Mechanism (CBM) Controller Panel Assembly (CPA) Removal

Dragon Ingress

USOS Window Shutter Close

Air sample collection using АК-1М adsorber in SpaceX

Robotic Workstation (RWS) Teardown

Dragon Arrival Photos

Transfer Center Stack

Cold Stowage Double Coldbag Unpack

Dragon Cargo Transfer Bag Retrieve

MERLIN Hardware Setup

Space Automated Bioproduct Lab, CO2 Incubator Installation

NanoRacks Platform-1 Module Install

TANGOLAB CARD CUBE REPLACE

Microgravity Expanded Stem Cell Ice Brick MERLIN Insertion

NanoRacks Module 9 Ops Session 1

XF305 Camcorder Setup

JAXA LT PCG Sample Preparation

Completed Task List Items

None

Ground Activities

All activities are on schedule unless otherwise noted.

Configure for Dragon capture

Return to nominal configuration

Three-Day Look Ahead:

Friday, 02/24: 66P Dock, 66P ingress, 66P transfer operations, SABLE CO2 Incubator Install, TangoLab-1 card replace, MERLIN3 install, Polar 1 transfer and install, Glacier 3 transfer and install, MESC first microscope ops,

Saturday, 02/25: RR-4 transfer

Sunday, 02/26: Microgravity Expanded Stem Cells (MESC)

QUICK ISS Status – Environmental Control Group: