They're the inseparable siblings, who were brought closer together by the sudden death of their famous father Steve ten years ago.

And on Thursday night, Bindi Irwin was right by her brother Robert's side as he made his late-night TV debut on The Tonight Show with Jimmy Fallon.

From backstage, Bindi took to Instagram to share a side-by-side photo comparing her brother and late father, who appeared on The Tonight Show several times when it was hosted by Jay Leno.

'You are more and more like Dad every day': An emotional Bindi took to Instagram after her brother's debut on The Tonight Show, sharing a side-by-side snap of her brother and late father who looked remarkably similar

'Tears in my eyes,'Bindi captioned the poignant post that showed the striking resemblance between father and son, who both introduced the hosts to giant snakes.

So proud of @robertirwinphotography,' the 18-year-old continued. 'You are more and more like Dad every day. I'm forever thankful to be your sister.'

Robert was just two years old when his father died after being stung by a stingray in 2006.

A lot of support: Bindi uploaded a second photo that showed Robert flanked by a team of his nearest and dearest who were ready to cheer him on

Bindi also shared a second snap from the green room backstage at Jimmy's show before Robert made his big debut.

The mirror selfie was taken by Bindi and featured Robert flanked by a team of supporters, including mother Terri and Bindi's boyfriend Chandler Powell, as well as two workers from Australia Zoo.

Crikey! Steve was a regular on The Tonight Show, appearing at least four times in the early 2000s when the program was hosted by Jay Leno

Robert's role model: Robert introduced Jay and actor Tobey Macguire to a huge python during a segment on the show in 2000

For his segment on the show, Robert appeared dressed in a khaki uniform, before introducing a terrified Jimmy to a number of animals.

First up, was a baby African Dwarf crocodile named Beetlejuice.

An unconventional guest! Robert Irwin appeared on The Tonight Show with Jimmy Fallon on Thursday, bringing along a red tail boa constrictor, much to the terror of the host

A tepid touch: Jimmy didn't seem too keen to cosy up with a crocodile by the name of Beetlejuice

'Would you like to give him a pat?' Robert asked a reluctant Jimmy, who only gave the reptile a tepid touch.

Jimmy seemed slightly less panicked by the next animal, a screaming armadillo by the name of Mickey.

Furry friends: Robert brought along a screaming armadillo much to the amusement of the comedian host

Sheer terror! Jimmy has no doubt encountered some difficult guests on his program, but it's unlikely any have caused him as much terror as the giant boa constrictor named Lady

But then came a giant boa constrictor, who Robert revealed went by the name of Lady.

'She's so cute,' assured the young animal enthusiast, to which Jimmy replied, 'No, they trick you with the names and it's like an evil thing.'

When the massive snake was brought out on stage Robert asked the comedian if he would like to have a hold.

'She's trying to kill me!'Two assistants had to rush on stage and untie the large reptile from the star

'No!' Jimmy replied, before the reptile was thrust across his arms anyway.

The giant snake then began to constrict around the comedian's arm as he yelped, 'She's trying to kill me!'

Lady then began to wrap around Robert's waist before two assistants had to come and remove the animal.

Steve's mini-me: Enthusiastic Robert was reminiscent of his late father as he introduced Jimmy to the animals

The funnyman was much more of a fan of the final animal- a sloth named Valentino.

And despite the terror, it appeared Jimmy enjoyed Robert's first visit to the studio.

'I love this, I love you. Thank you for bring all these fun animals and, gosh, what a treat, man!' Jimmy said as he wrapped the segment.