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One of Hollywood's funniest couples, Will Arnett and Amy Poehler, are separating after nine years of marriage, their publicist confirms to ABCNews.com.

Arnett, 42, and Poehler, 40, have two sons together, Archie, 3, and Abel, 2. The two have appeared together on screen in a number of projects, including "Arrested Development," "Blades of Glory," and "Monsters vs. Aliens." In 2010, Arnett guest starred in an episode of Poehler's NBC comedy "Parks and Recreation."

The two may cross paths at the Emmy Awards on Sept. 23. Poehler is nominated for best lead actress in a comedy and best writing, both for "Parks and Rec." Arnett is nominated for best guest actor in a comedy for playing nefarious exec. Devon Banks on "30 Rock."

This was the first marriage for Poehler and the second for Arnett, who married actress Penelope Ann Miller in 1994. They divorced a year later.