Labor is holding its breath for its first major legislative defeat in the state parliament - while breathing a sigh of relief a loss could mean a stronger campaign in seats vulnerable to the green vote.

While still holding out for a win on its vegetation management agenda, the minority government still does not know if it holds the vote of key independent Billy Gordon, who is facing pressure from indigenous groups, and Peter Wellington, who has voted both ways on the issue in the past.

Cook MP Billy Gordon/

Mr Wellington remains uncomfortable with the reverse onus of proof aspect of the bill, which sees landholders having to prove they did not clear land instead of the department proving they did, which Labor has so far refused to budge on amending, despite also facing pressure from the Queensland Legal Society.

With the Katters having tied their vote to the LNP, Labor is headed into the chamber on Wednesday unsure of where the vote, scheduled for Thursday after a two day debate, will fall.