Rosie O'Donnell has made a new, foul-mouthed, attack on presidential candidate Donald Trump on Wednesday, calling the Republican front-runner a 's*** stain'.

She launched the tirade while appearing on stage at a Boy George and Cyndi Lauper concert in New York.

The 54-year-old comedian opened her set at the Beacon Theatre by telling the audience that she was 'depressed because I f*****g hate that orange piece of s***'.

O'Donnell, who has been at the receiving end of Trump's insults herself, said in the almost two-minute rant that her therapist told her to not focus on the Republican front-runner.

'Well I would rather give birth to a flaming iguana while taking a s***,' she recalled saying. 'I hate him. I hate him.'

WATCH O'DONNELL'S RANT

Rosie O'Donnell launched a foul-mouth rant against Donald Trump on Wednesday at New York's Beacon Theatre

The 54-year-old comedian opened her set by telling the audience that she was 'depressed because I f*****g hate that orange piece of s***'. Trump is campaigning and on Wednesday was in Anaheim, California

The mother-of-five insisted she was not going to say his name before grabbing her crotch and saying, 'Eat me, Donald'.

O'Donnell took the stage at a Boy George concert, where '80s popstar Cydi Lauper also made an appearance.

The crowd mostly laughed as she delivered her caustic one-liners about Trump.

When mentioning someone who was an 'enemy for years who's now my friend', she described his relationship with Trump.

'He is friends with the s*** stain, which worries me,' she says to the crowd, before calling Trump a 's*** stain with a tinge of orange'.

A man who was in the audience told Daily Mail Online that he quickly pulled out his camera to document the foul-mouthed rant.

'I took out my phone and started recording when she said "I'm depressed because I f***ing hate that orange piece of s***",' he said.

'Rosie's routine was just jaw-dropping,' he added. 'She sounds like she is obsessed with Donald Trump and it was what she spent most of her very short act talking about.'

Though several audience members let out loud cheers during O'Donnell's act, not everyone was pleased, the audience member said.

'Not everyone was impressed,' he said. 'People around me were shaking their heads in disagreement.

Obscene image: At one point during her set, after saying she wouldn't use the Republican front-runner's name, she grabbed her crotch and said, 'Eat me, Donald'

Reason to be there: O'Donnell's tirade was at a Cyndi Lauper and Boy George concert. One theatergoer told Daily Mail Online an audience member shouted at O'Donnell: 'Get off and let Cyndi sing.'

'One man near me turned to his wife turned and said, "I really hope Donald gets elected so she keeps her promise and moves to Canada". Another lady was shouting, "Get off and let Cyndi sing".'

O'Donnell and Trump have been feuding since 2006, when he called her a 'loser' after she questioned his decision to not fire the then-Miss USA Tara Conner over her reported drug use.

The feud erupted again in 2011 when O'Donnell announced she was engaged to marry her now ex-wife Michelle Rounds.

At the time, Trump tweeted; 'I feel sorry for Rosie 's new partner in love whose parents are devastated at the thought of their daughter being with @Rosie--a true loser.'

She responded on Twitter by calling him a 'real a**.'

Then, after her heart attack in August 2012, Trump tweeted at O'Donnell saying he was thinking about her and hoped she got better, to which O'Donnell replied; 'well thank u donald - i must admit ur post was a bit of a shock ... r u trying to kill me ? xx'

The two were back at it again however in 2014 when Trump attacked O'Donnell for getting weight-loss surgery, saying she had previously criticized Star Jones for doing the same things years earlier.

Later that same year when it was revealed O'Donnell would be returning to The View, Trump wrote on Twitter; 'Rosie is crude, rude, obnoxious and dumb - other than that I like her very much!'

These comments were just a few of the ones that Fox News host Megyn Kelly brought up to Trump at the first Republican debate last August when asked about his use of the words 'fat pigs', 'dogs', 'slobs' and 'disgusting animals' when describing women.

'Only Rosie O'Donnell,' Trump said in response to the statement.

O'Donnell tweeted later that night; 'try explaining that 2 ur kids.'

One of her children is estranged from her. Chelsea, 18, was declared missing by O'Donnell last year but it then emerged she had quit the family home in Nyack, New York, to live with a boyfriend.