For the study 3,416 normal healthy male subjects between 20–30 years of age were recruited. As menstural and pre-menstural phases would have confounding effects during the determination of Prakriti assessment, the females were excluded. ‘The subjects belonged to diverse ethnic and linguistic groups, and inhabited different geographical regions. The health status of every individual was ascertained by modern as well as Ayurvedic methods.’



Then, of the total 3,416 individuals evaluated, 971 having dominance of one Prakriti were taken and through quality control, 262 individuals (94 Vata-dominant, 75 Pitta-dominant and 93 Kapha-dominant) with the highest proportion of one predominant Prakriti were randomly selected. Subjected to further quality control (QC) a final 245 were selected.



Starting with 7,91,186 SNP markers, they zoomed in on 4,05,782 SNPs. ANI-ASI deep ancestry structure of Indian population was also taken into account. The analysis now reveals 52 SNPs as ’genuine characteristics of Prakriti and not derived from ancestry’.