Salk Institute researchers have discovered a startling feature of early brain development that helps to explain how complex neuron wiring patterns are programmed using just a handful of critical genes.

The findings may help scientists develop new therapies for neurological disorders, such as amyotrophic lateral sclerosis (ALS), and provide insight into certain cancers.

The Salk researchers discovered that only a few proteins on the leading edge of a motor neuron’s axon its outgoing electrical “wire” — and within the extracellular soup it travels through — guide the nerve as it emerges from the spinal cord.

These molecules can attract or repel the axon, depending on the long and winding path it must take to finally connect with its target muscle.

“The budding neuron has to detect the local environment it is growing through and decide where it is, and whether to grow straight, move to the left or right, or stop,” says the study’s senior investigator, Sam Pfaff, a professor in Salk’s Gene Expression Laboratory and a Howard Hughes Medical Institute investigator.

The brain contains millions of times more neuron connections than the number of genes found in the DNA of brain cells. This is one of the first studies to try and understand how a growing neuron integrates many different pieces of information in order to navigate to its eventual target and make a functional connection.

“We focused on motor neurons that control muscle movements, but the same kind of thing is going on throughout embryonic development of the entire nervous system, as millions of axons make trillions of decisions as they move to their targets,” he says. “It is the exquisite specificity with which they grow that underlies the basic architecture and proper function of the nervous system.”

Better understanding of nervous system diseases and cancer

These findings might eventually shed new light on a number of clinical disorders related to faulty nerve cell functioning, such as ALS (Lou Gehrig’s disease), says Dario Bonanomi, a post-doctoral researcher in Pfaff’s laboratory. “These are the motor neurons that die in diseases like Lou Gehrig’s disease and that are linked to a genetic disorder in children known as spinal muscle atrophy.”

“It is also a jumping off point to try and understand the basis for defects that might arise during fetal development of the nervous system,” he added. “A better understanding of those signals might help to be able to regenerate and rewire circuits following diseases or injuries of the nervous system.”

The researchers say the study also offers insights into cancer development, because Ret, a protein the researchers found to be crucial to the “push and pull” signaling system, is also linked to cancer. Mutations that activate Ret are linked to a number of different kinds of tumors.

Ephs, the other protein described in the study, have also been implicated in cancer, Pfaff says.

“This study suggests that the way cells detect signals in their environment is likely a universal strategy,” he says, “and we know that genes and proteins known to function primarily during embryonic development have been linked to cancer.”

“Controlling neuronal growth requires very potent signaling molecules, and it makes sense they would be linked to disease,” Pfaff says. “We hope our findings help further unravel these connections.”

The study was funded by the National Institute of Neurological Disorders and Stroke and by the Howard Hughes Medical Institute.

Ref.: Dario Bonanomi et al., Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals, Cell, 2012 [DOI: 10.1016/j.cell.2012.01.024]