Compounds In Testing

Each material is given in the diet at the following levels (part per million (PPM) by weight).

Cohort 1: C2004 Compound Concentration in food Age at treatment initiation Aspirin 20 ppm 4 months NFP 200 ppm 4 months NDGA 2,500 ppm 9 months 4-OH-PBN 315 ppm 4 months

Cohort 2: C2005 Compound Concentration in food Age at treatment initiation CAPE 30 ppm 4 months CAPE 300 ppm 4 months Enalapril Maleate 120 ppm 4 months Rapamycin 14 ppm 20 month

Cohort 3: C2006 Compound Concentration in food Age at treatment initiation Rapamycin 14 ppm 9 months Simvastatin 12 ppm 10 months Simvastatin 120 ppm 10 months Resveratrol 300 ppm 12 months Resveratrol 1200 ppm 12 months

Cohort 4: C2007 Compound Concentration in food Age at treatment initiation Resveratrol 300 ppm 4 months Oxaloacetic acid 2200 ppm 4 months Green tea extract 2000 ppm 4 months Curcumin 2000 ppm 4 months Medium Chain Triglyceride Oil 60000 ppm 4 months

Cohort 5: C2009 Compound Concentration in food Age at treatment initiation 17α-Estradiol 4.8 ppm 10 months Methylene Blue 28 ppm 4 months Acarbose 1000 ppm 4 months Rapamycin_LoPhase II 4.7 ppm 9 months Rapamycin_MidPhase II 14 ppm 9 months Rapamycin_HiPhase II 42 ppm 9 months

Cohort 6: C2010 Compound Concentration in food Age at treatment initiation Fish Oil 15000 ppm 9 months Fish Oil 50000 ppm 9 months NDGA Lo_Phase II 800 ppm 6 months (M only) NDGA Med_Phase II 2500 ppm 6 months (M only) NDGA Hi_Phase II 5000 ppm 6 months (M & F)

Cohort 7: C2011 Compound Concentration in food Age at treatment initiation Bile Acids 5000 ppm 5 months Metformin 1000 ppm 9 months Metformin + Rapamycin 1000 ppm M + 14 ppm R 9 months Protandim® * 600 ppm 10 months 17α-Estradiol 14.4 ppm 10 months

*Protandim® was fed to one group of mice – the Protandim® was increased from 600 ppm to 1200 ppm when the mice reached 17 months of age.

Cohort 8: C2012 Compound Concentration in food Age at treatment initiation INT-767 FXR/TG5R agonist 180 ppm 10 months Acarbose 1000 ppm 16 months HBX (2-(2-hydroxyphenyl)-benzoxazole) 1 ppm 15 months NDGA cross-section study: Males 2500 ppm 13 months NDGA cross-section study: Females 5000 ppm 13 months

Cohort 9: C2013 Compound Concentration in food Age at treatment initiation Ursolic Acid 2000 ppm 10 months Acarbose Phase II 2500 ppm 8 months Acarbose Phase II 1000 ppm 8 months Acarbose Phase II 400 ppm 8 months

Cohort 10: C2014 Compound Concentration in food Age at treatment initiation Supplemental glycine 80,000 ppm 9 months TM5441 – inhibitor of PAI-1 60 ppm 11 months Inulin 600 ppm 11 months Aspirin 60 ppm 11 months Aspirin 200 ppm 11 months

Cohort 11: C2015 Compound Concentration in food Age at treatment initiation 17-dimethylaminoethylamino-17-demethoxygeldanamycin hydrochloride (17-DMAG) 30 ppm 6 months MitoQ 100 ppm 7 months Minocycline 300 ppm 6 months β-guanadinopropionic acid (B-GPA) 3300 ppm 6 months Rapamycin – intermittent Phase II 42 ppm 20 months

Cohort 12: C2016 Compound Concentration in food Age at treatment initiation MIF098 240 ppm 8 months Nicotinamide riboside 1000 ppm 8 months Canagliflozin - SGLT2 inhibitor 180 ppm 7 months Candesartan Cilexetil 30 ppm 8 months Geranylgeranyl acetone 600 ppm 9 months Hydrogen Sulfide - SG1002 240 ppm 19 months Phase II - 17αEstradiol started in mid-life 14.4 ppm 16 + 20 months

Cohort 13: C2017 Compound Concentration in food Age at treatment initiation 1,3-butanediol 100,000 ppm 6 months Captopril 180 ppm 5 months L-Leucine 40,000 ppm 5 months PB125 100 ppm 5 months Sulindac 5 ppm 5 months Syringaresinol 300 ppm 5 months Phase II - Rapamycin/Acarbose 14.7 ppm/1000 ppm 9 + 16 months

Cohort 14: C2018 Compound Concentration in food Age at treatment initiation Fisetin on + cycling (3d on/11d off) 600 ppm 20 months Hydrogen Sulfide - SG1002 midlife 240 ppm 18 months