Brisbane City Council's LNP administration will vote down a public push for protected bike lanes on a busy inner-city road on Tuesday, despite receiving one of the biggest petitions in its history calling for their installation.

Sylvan Road in the city's inner west is Brisbane's busiest unprotected cyclist thoroughfare, according to the advocacy group CBD BUG, which said audits performed in 2014 revealed 40 per cent of all traffic using the road in peak hour were cyclists.

A protected bike path in Melbourne. Credit:Craig Sillitoe

Head petitioner, CBD BUG co-convenor Donald Campbell, accused the council of ignoring the safety concerns of 755 people who signed an online petition last year calling for the installation of protected bike lanes along the one kilometre stretch of road connecting Milton Road and Coronation Drive.

"It's very disappointing, we could say they need to develop some political testicles because it's really coming down to the fact they are losing 20 to 30 votes by losing car spaces versus potentially saving someone from injury or death," he said.