Increased apoptotic rate in bone-marrow mononuclear cells might be related to increased inflammatory cytokines.

Apoptosis and the advanced oxidation protein products were increased after chronic fructose intake.

Abstract

Background Measuring of oxidative stress in peripheral blood mononuclear cells is a suitable model of dietary induced systemic oxidative stress. Thus, we aimed to evaluate whether a chronic high fructose intake could induce oxidative damage in peripheral blood and bone marrow mononuclear cells of rats.

Methods Animals were randomly assigned to the following groups: Control group (standard rat chow and tap water n = 8), and Fructose group (standard rat chow and a 10% fructose solution in the drinking water n = 8). Reactive oxygen species and cytokines were measure using flow cytometry in peripheral blood and bone-marrow mononuclear cells. Apoptotic cell death and the advanced oxidation protein products (AOPP) were also determined.

Results We observed a significant increase in ROS production in peripheral blood mononuclear cells of fructose group as compared to control rats. Apoptosis and the AOPP were higher in those animals underwent high fructose intake. Serum levels of IL-6 and IL-12 were also increased after 12 weeks of high fructose intake.