The rift within One Nation is growing increasingly public with party leader Pauline Hanson criticising her colleague's lack of political experience.

Senator Hanson confirmed to the ABC that she had not spoken to Senator Rod Culleton since the final parliamentary sitting day last week, when he broke away from the party to vote on a 13 per cent backpacker tax proposal.

Describing loyalty as "very important", Senator Hanson said Senator Culleton was not a team player and she could no longer work with him.

She told the ABC the WA Senator should have taken her advice.

"I've been on the political scene now since 1994 — that's 22 years," she said.

"Rod Culleton's been in here since he was elected July 2. He has to understand I've got a lot of political experience.

"I just think that he should have listened to not only me, but also the other couple of senators that I have."

Senator Culleton has been in the High Court this week and awaits a ruling rule on the validity of his election.

Speaking to reporters on Wednesday, he would not be drawn on his future with One Nation.

When asked if he had heard her public criticisms, he responded that "I haven't heard her slag me off".