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A benefit fund for the families of cops and firefighters killed in the line of duty will support the wife and young daughter of firefighter William Tolley, who was killed in a freak accident in Queens on Thursday.

Answer the Call will give $25,000 to Tolley’s widow Marie and 8-year-old daughter Bella, according to an April 21 announcement from the organization.

“Men and women of our Police and Fire Departments like William Tolley put their lives on the line each day to keep us safe and it is our responsibility to help ensure their families have the support they need when tragedy occurs,” said Answer the Call board chairman Stephen Dannhauser. “As New Yorkers ourselves, we vow to honor Tolley and support his family from this day forward.”

In addition, the group plans to give the mother and daughter an annual stipend, which can vary yearly based on amount of donations the charity receives, according to a spokeswoman.

Tolley fell five stories from a Queens apartment building while doing “routine” work after he and fellow Bravest extinguished a blaze there at about 3 p.m., according to Fire Commissioner Daniel Nigro.

Tolley was taken to Wyckoff Heights Medical Center, where he died of his injuries. The FDNY is still investigating.

Mayor de Blasio pledged Thursday that the city would also take care of Tolley’s loved ones.

“And, to his family, we say we will stand with you not only today, but in the days ahead, and for years and years to come,” he said at a press conference. “This is what the FDNY does — stays by families through everything that comes ahead.”