Michelle Payne's hopes of a second Melbourne Cup on Prince Of Penzance appear to be over after it was revealed she will not be aboard the cup winner when he has his return run at Caulfield.

Payne will not partner Prince of Penzance when he resumes in the Memsie Stakes at Caulfield on August 27.

The news appears to have angered Payne, who vented her frustration on Twitter before deleting the tweet.

"Not anymore I'm done, why work your arse off for people who don't appreciate what you do and write you off anyway #moretolife," Payne said.

Payne was seriously injured in a race fall at Mildura in May and underwent pancreatic surgery.

After the fall, she said she had lost her memory of winning the Melbourne cup and "had to Google it".

She recently made a return to riding trackwork and was looking forward to continuing her association with Prince Of Penzance, which made the jockey a household name.

Payne has applied for a training licence and has hopes of combining training and riding when her application is granted.

Sorry, this video has expired Michelle Payne falls during race in Mildura

AAP