Mr. De Niro’s statement seemed to suggest that this was the first time he has expressed a preference that a particular film be shown at the festival.

“Grace and I have a child with autism,” he wrote, referring to his wife, Grace Hightower De Niro, “and we believe it is critical that all of the issues surrounding the causes of autism be openly discussed and examined. In the 15 years since the Tribeca Film Festival was founded, I have never asked for a film to be screened or gotten involved in the programming. However this is very personal to me and my family and I want there to be a discussion, which is why we will be screening VAXXED.”

Within hours his statement, posted on Facebook, generated hundreds of comments.

The trailer for the film, filled with dramatic music, opens with the words “Are Our Children Safe?” on a black screen with billowing smoke that appears to be coming from a syringe. The trailer includes the suggestion that a “whistle-blower” from the Centers for Disease Control and Prevention would say that the organization “had committed fraud” and “that they knew that vaccines were actually causing autism.” Mr. Wakefield appears in the trailer saying, “Wow, the C.D.C. had known all along there was this M.M.R. autism risk.”