Article type: Research Article

Authors: Jiang, Boa; * | Zheng, Shanglia | An, Lijiaa | Guo, Jianlia | Asakawa, Tetsuyab; c; *

Affiliations: [a] School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning, China | [b] Department of Neurosurgery, Hamamatsu University School of Medicine, Handayama, Hamamatsu-city, Shizuoka, Japan | [c] Research Base of Traditional Chinese Medicine Syndrome, Fujian University of Traditional Chinese Medicine, Fuzhou, China

Correspondence: [*] Corresponding author: Bo Jiang., Ph.D., School of Life Science and Biotechnology, Dalian University of Technology, Dalia No. 2 Linggong Road, Ganjingzi District, Liaoning, 116024, China. Tel.: +86 411 84706355; Fax: +86 411 84706355; E-mail: [email protected] and Tetsuya Asakawa, M.D., Ph.D., Department of Neurosurgery, Hamamatsu University School of Medicine, Handayama, 1-20-1, Higashi-ku, Hamamatsu-city, Shizuoka, 431-3192, Japan. Tel.: +81 53 435 2283; Fax: +81 53 435 2282; E-mail: [email protected].

Abstract: BACKGROUND:Blueberry juice is a common drink for relieving fatigue, but its mechanisms of action are unclear. OBJECTIVE:This study aimed to validate changes in the dopaminergic system, especially changes in dopamine transporter (DAT) levels, in a fatigued state and assess whether such fatigue-related changes can be ameliorated by blueberry juice. METHODS:We investigated the expression of striatal DAT and tyrosine hydroxylase (TH) in mice subjected to a forced swimming fatigue task and subsequently administered blueberry juice (anthocyanin), caffeine, or Red Bull (caffeine + taurine) to assess DAT and TH changes in the nigrostriatal pathway related with fatigue as well as their relief. RESULTS:Striatal DAT and TH expression was downregulated by fatigue and upregulated by treatment with blueberry juice, caffeine, and Red Bull. In addition, DAT and TH expression exhibited positive correlations with fatigue-related motor performance. CONCLUSIONS:We provided evidence of compensatory responses of striatal DAT and TH in the fatigued state, and treatments to relieve fatigue might upregulate the expression of both striatal DAT and TH. Moreover, small amounts of blueberry juice exerted similar effects as large amounts, suggesting that anthocyanin has relatively strong anti-fatigue effects.

Keywords: Fatigue, anthocyanin, tyrosine hydroxylase, dopamine transporter, forced swimming test, blueberry juice

DOI: 10.3233/JBR-180350

Journal: Journal of Berry Research, vol. 9, no. 2, pp. 321-332, 2019