Two Guide leaders who had raised safeguarding concerns about the organisation’s transgender policy have been expelled and had their units closed down.

Dozens of children face disappointment because there is no one else to run the units. The expelled leaders say they will take legal action against Girlguiding if their removals are upheld.

Helen Watts, one of 12 leaders who signed a letter to The Sunday Times in April asking for a review of the policy, was told on Friday that her membership was being terminated after more than 15 years with the Guides.

At least one other signatory, based in the northwest of England, was expelled. Documents seen by The Sunday Times suggest disciplinary investigations have been launched against at least five Guide leaders.