The boss of toll road giant Transurban has lashed Opposition Leader Matthew Guy and the Greens for being hypocritical and playing "cheap politics" by trying to kill off the West Gate Tunnel project.

The two parties are preparing to use their combined majority in Victoria's upper house next week to revoke planning approval for the $6.7 billion project.

Mr Guy put Transurban on notice in late December, warning in writing that the Coalition would vote "at the earliest available parliamentary opportunity" to revoke the planning scheme amendment needed for the project to proceed.

Unperturbed, the Andrews government began construction on the project last month.

The project links the West Gate Freeway in Spotswood with CityLink in Docklands, via a six-lane tunnel under Yarraville.