The veteran faced a race against time to be fit in time for the second leg on Wednesday, but his club have confirmed he will play no part in the game

Andrea Pirlo has been ruled out of the second leg of Juventus ' last-16 Champions League tie with Borussia Dortmund on Wednesday.

The 35-year-old picked up a calf injury in the first leg in Turin and has not featured since, but still retained hope of winning his battle with fitness to play a part in the game.

However, the club have now confirmed the match has come too soon by releasing a statement on their official website.

"Andrea Pirlo: the ultrasound scan showed a good evolution to the healing of the wound, but not enough to make him available for the trip to Dortmund.

"The midfielder will therefore continue his rehabilitation program."

Pirlo's absence means Claudio Marchisio is likely to start alongside Arturo Vidal and Paul Pogba in the Juventus midfield.

Massimiliano Allegri's side lead 2-1 from the first leg, in which goals from Carlos Tevez and Alvaro Morata secured victory.