There are five new presumptive positive cases of coronavirus in Massachusetts as of Saturday, according to the Massachusetts Department of Health.

The total number of COVID-19 cases in the state is now at 13, with one confirmed case and 12 presumptive positive cases. COVID-19 is the disease caused by a new coronavirus.

The new cases include four people from Middlesex County: one woman in her 40s, one woman in her 50s, one man in his 40s and another man in his 60s. The fifth case is a man from Berkshire County in his 60s, according to DPH.

Of the five cases, three had a direct connection to Biogen. The Cambridge-based biotech company held a meeting last week and on Thursday, the company announced three people who attended the meeting had tested positive for the novel coronavirus.

One person among the five new cases had recent international travel that included northern Italy. The fifth case is still under investigation, DPH said.

The presumptive positive tests are being sent to the U.S. Centers for Disease Control and Prevention for confirmation.

Health officials said the risk of COVID-19 to the general public in Massachusetts remains low.

As of Wednesday, 719 people have been subject to self-quarantine in Massachusetts because of COVID-19. Of those people, 470 people have completed monitoring and are no longer quarantined, leaving 249 people currently in quarantine.

On Friday, state officials announced new presumptive positive cases of COVID-19, which then brought the state’s tally to eight cases.

Boston Mayor Marty Walsh said on Friday that there are three presumptive cases of the virus among Boston residents that trace back to the Biogen company meeting, which took place at the Marriott Long Wharf.

A UMass Boston student was the first person in the state to be diagnosed with COVID-19.

Other presumptive cases in the state include a woman in her 20s from Norfolk County and a woman in her 60s from Middlesex County.

In the town of Norwood, 30 people, including 11 town employees, are under self-quarantine after attending a party last week with a resident who has since tested positive for the virus.

Concerns about the virus have had a ripple effect across the state.

Multiple Springfield Public Schools staffers were quarantined after recently traveling to Italy. Plymouth Public Schools canceled classes Friday to clean district school buildings and buses over fears of the coronavirus, and Wellesley’s school district dismissed students early following a COVID-19 diagnosis.

The outbreak of the respiratory illness began in Wuhan, China. Since then, cases have spread around the world.

The CDC as of Friday, March 6 has reported 164 total cases of COVID-19 in the U.S., with 11 deaths and 19 states reporting cases of the illness. Of those 164 cases, 36 are travel-related, 18 are from person-to-person spread, and 110 are under investigation, according to the CDC.

Related Content: