A one-of-a-kind sports car worth $300,000 has been stolen from a garage in one of Melbourne's most exclusive suburbs.

The blue McLaren 12C Spyder was stolen by two or three men, Victoria Police said, just before 1.30am on Wednesday morning.

CCTV footage shows the sports car being slowly driven out of the driveway in Toorak.

This McLaren Spyder was the only one of its type and colour in Australia. (Victoria Police)

The McLaren has a distinctive three-digit numberplate of 12C.

It was last seen on South Road in Moorabbin shortly after the theft.

This particular McLaren is the only one of that colour and type in Australia.

The McLaren Spyder is worth $300,000. (Victoria Police)

Vehicle Crime Squad detectives are investigating the theft.