adb12606-sup-0004-Supplementary_figure_legends.docWord document, 26.5 KB

Figure S1. The effect of the synthetic cannabinoids and Δ9‐THC on body temperature (n = 7–10). (A) 4 mg/kg of AB‐FUBINACA led to a significantly reduced rectal temperature among the mice (t(9) = 3.22, p = 0.11). (B) Mice which received AB‐CHMINACA at the doses of 0.25, 0.5 and 1 mg/kg experienced a significant decreased body temperature (t(9) = 5.01, p = 0.001, t(8) = 6.93, p = 0.000 and t(6) = 5.71, p = 0.001, respectively). (C) The vehicle used to dissolve PB‐22 caused a slight but significant elevation of body temperature (t(9) = −4.93, p = 0.001). Mice which were administered PB‐22 at the doses of 0.1, 0.2 and 0.4 mg/kg suffered from significantly lowered rectal temperature (t(8) = 4.51, p = 0.002, t(8) = 4.51, p = 0.003 and t(6) = 9.52, p = 0.000, respectively). (D) 2.5 mg/kg of Δ9‐THC caused a slight but significant elevation of the body temperature of the mice (t(9) = −2.88, p = 0.018). However, 25 mg/kg of Δ9‐THC led to a significant reduction of the rectal temperature of the mice (t(8) = 8.54, p = 0.000). p* < 0.05, p** < 0.01, p*** < 0.001 Figure S2. Dose response curve of Δ9‐THC (n = 9–10). Administration of Δ9‐THC was found to induce a clear antinociceptive effect in the hotplate assay. Following intraperitoneal (i.p.) injection Δ9‐THC led to an antinociceptive effect in a dose‐dependent manner, with an ED50 value of 60.49 mg/kg (95 percent CL, 50.97–75.43) Figure S3. The effect of the synthetic cannabinoids and Δ9‐THC on anxiety‐like behaviour as measured in the elevated plus maze. (A) 3 mg/kg of AB‐FUBINACA caused decreased levels of anxiety while 4 mg/kg of AB‐FUBINACA demonstrated the opposite effect and resulted in reduced time spent in the open arm (F(5,36) = 6.13, p = 0.000). (B) Mice which received AB‐CHMINACA at 1 mg/kg spent significantly longer time in the open arm of the maze compared with all other groups (F(5,39) = 5.57, p = 0.001). (C) PB‐22 caused significant decreased levels of anxiety at the doses of 0.05, 0.1 and 0.4 mg/kg as compared with saline and vehicle (F(5,32) = 8.49, p = 0.000). (D) Mice which were administered 25 mg/kg of Δ9‐THC spent significantly longer time in the open arm of the maze compared with all other groups (F(5,50) = 3.41, p = 0.01). p*/#/$/¥/^ < 0.05, p**/##/$$/¥¥/^^ < 0.01, p***/###/$$$/^^^ < 0.001