Mycobacterial infections that are transmitted by acupuncture are an emerging problem. Mycobacterium chelonae is one of the rapid-growing atypical mycobacteria. In a healthy person, cutaneous infection with M chelonae is known to occur after invasive procedures like surgery and acupuncture. Herein, we present a case of a 66-year-old woman with 3-month history of multiple erythematous plaques and nodules with ulceration on the back, left hip and left shin at the site of acupuncture. At the first visit, her history of acupuncture was not disclosed, and multiple furunculosis were clinically suspected. Initial treatment 750 mg of cefaclor for 2 weeks and incision and drainage twice failed to improve the skin lesions. Hence, punch biopsy of a purulent nodule was done. The histopathologic analysis showed both polymorphonuclear microabscess and granulomatous inflammation. PAS, gram staining and routine bacterial culture were negative. Acid-fast bacilli (AFB) were detected by AFB stain and polymerase chain reaction revealed M chelonae. An additional careful history taking revealed that she had gotten acupuncture prior to the breakout of her skin lesions. The patient has been treated with 500 g of clarithromycin and 200 mg of doxycycline for 2 months and her condition has improved considerably. Despite of the increasing popularity of acupuncture in Korea, cutaneous infection is still not effectively controlled. Education of the measures for proper infection control, including aseptic practice, is necessary.