Abstract The outbreak of white-nose syndrome in North American bats has resulted in massive data collection efforts to characterize the fungus, Pseudogymnoascus destructans. Wing biopsies routinely are collected from live bats, placed in agar media to culture the fungus, and ultimately discarded. We tested whether these discarded tissues represent a viable source of host bat DNA. We found no difference in DNA concentration and no reduction of DNA quality between samples that were extracted immediately compared to samples placed in agar for fungal culture. Although recovered quantities were low, concentrations increased using a cleanup kit. Our study suggests samples collected from live bats can be leveraged across disciplines to further our understanding of bat genetics and the impact of white-nose syndrome.

White-nose syndrome (WNS) has killed millions of bats since it was first detected in 2006 (US Fish and Wildlife Service 2012), prompting efforts to collect samples to better understand this fungal outbreak. Sampling hibernating bats presents interesting challenges. Animals are slow moving and aggregated allowing for multiple samples from a small area. However, unnecessary arousals result in significant energy expenditures (Thomas et al. 1990; Speakman et al. 1991) so number of sampling events should be minimized. Sampling logistics are further complicated because humans can spread fungal spores and cave access often is restricted (US Fish and Wildlife Service 2011). Therefore, research efforts are constrained in the number of animals and locations that can be sampled and achieving required sample sizes is difficult.

The small size of bats must be considered when collecting tissue. Wing biopsies are routinely used for diagnostics and confirmation of WNS. Federal guidelines recommend no more than two, 3–5 mm wing punches should be taken from each bat (http://www.nwhc.usgs.gov/disease_information/white-nose_syndrome/USGS_NWHC_Bat_WNS_submission_protocol.pdf). The USGS National Wildlife Health Center recommends using one biopsy from each wing to detect fungal presence either through direct culture or PCR methods, precluding additional genetic sampling.

To maximize tissues collected from live bats, we determined if samples collected for P. destructans research can be utilized for additional research projects on bat genetics. Tissues are placed in agar medium to determine fungal diversity via culturing and traditionally discarded once cultures are identified. Here we measured DNA quantity and quality of tissue samples that underwent fungal culture compared to fresh samples to determine their utility for genetics research.

We obtained samples from 15 bats collected for the Illinois Department of Public Health rabies surveillance program. We replicated biopsy procedures, collecting two 3 mm wing biopsies (Fig. 1a). DNA from one wing punch was extracted immediately, while the other was placed in either Sabourauds or Potato Dextrose Agar and stored at 7 °C for 2 weeks, replicating the culture protocol used for WNS detection (Fig. 1b). After 2 weeks, tissues were removed from agar and placed in 95 % ethanol. Samples are hereafter referred to as ‘fresh’ and ‘agar’. Fig. 1 a Wing biopsies taken from archived bat specimen. b Bat biopsy fungal cultures Full size image

We extracted DNA using a Quick gDNA miniprep kit (Zymo Research Corp., Irvine, CA, USA) following manufacturer’s protocol. We eluted samples in 50 µL volumes and estimated total DNA quantity and quality using a Nanodrop® 2000 (Nanodrop Technologies, Wilmington, DE, USA). We measured total DNA yield and purity following methods of Green et al. (2013). We used Zymo Clean & Concentrator kit (e.g. ‘cleanup kit’) to purify DNA and repeated the measurements and calculations for DNA quantity and quality. We verified recovery of host DNA by amplifying PCR products using microsatellite loci EF5, EF6, EF21 (Vonhof et al. 2002), B22, E24, and F19 (Castella and Ruedi 2000); products from fresh and agar tissues were within the expected size ranges.

We tested whether data met normality assumptions using a Kolmogorov–Smirnov test before comparing quantity and quality of DNA from fresh and agar samples, before and after cleanup. We tested for differences in DNA quantity and quality using a t test and compared average concentrations and absorbance ratios between samples before and after cleanup using an ANOVA. All statistical analyses were completed in SAS v.9.3 (Cary, NC, USA).

All data met normality assumptions. We found no difference in DNA quantity between fresh and agar samples whether using DNA prior to cleanup (t 28 = 0.49; P = 0.63) or post cleanup (t 28 = 1.12; P = 0.27). We also found no difference in DNA quality between fresh and agar samples prior to cleanup (t 28 = −1.20; P = 0.24) or post cleanup (t 28 = 1.56; P = 0.13). Average DNA concentrations of both fresh and agar samples increased after cleanup kit processing (Table 1; F = 8.3, P < 0.01) independent of whether they were fresh or agar. Purity ratios did not change after using the cleanup kit (F = 0.72, P = 0.49). Table 1 Total amount of DNA and purity ratios recovered from fresh and agar tissues Full size table

Host DNA can be recovered from wing biopsies placed in agar for P. destructans detection with no reduction in quantity or quality, opening research possibilities utilizing samples that would otherwise be discarded. The quantity of recovered DNA was suitable for downstream applications, including microsatellites, which have been amplified from bats using 10–25 ng of genomic DNA (Trujillo and Amelon 2009; Burns et al. 2012). DNA recovery of fresh and agar samples falls within the suitable range for microsatellite amplification even without post-extraction cleanup. The cleanup kit concentrated samples and should be used when working with small tissue samples because higher concentrations provide more control during downstream optimization.

Given the complexities involved with sampling bat populations, tissues collected for fungal detection represent a valuable genetic resource. Although molecular approaches require intensive sampling efforts, they allow researchers to ask new questions at resolutions previously unavailable (Kelly et al. 2010; Green et al. 2014). This research will pave the way for interdisciplinary partnerships to increase the knowledge produced from a single sample and for molecular approaches to enhance our understanding of WNS ecology (Archie et al. 2009).

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Acknowledgments Research supported by USFWS Federal Aid in Wildlife Restoration Project (W-146-R) and University of Illinois Office of the Vice Chancellor for Research. The authors thank Daniel Raudabaugh and Nicole Conway for their assistance.

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