Senator Jacqui Lambie's chief of staff has been expelled from the Palmer United Party (PUP), in a move that will inflame existing tensions within the balance-of-power voting bloc.

Party leader Clive Palmer has accused Senator Lambie of acting as "Rob Messenger's mouthpiece" and suggested she should challenge for the leadership if she is unhappy.

"Everything Senator Lambie says is really coming from her chief of staff," Mr Palmer said in a statement.

"[Wednesday] night our executive met and we have agreed to expel Rob Messenger from the party on the grounds of making false and misleading statements about our senators."

Mr Palmer said he had not spoken to Senator Lambie for a fortnight.

"I haven't spoken to the woman for two weeks. She doesn't return my calls," he said.

"I have called her on six occasions and they just don't answer their telephone."

He has also accused Mr Messenger, who is a former state politician in Queensland, of trying to start his own political party.

Mr Messenger has been working as Senator Lambie's chief of staff since her election, but was previously a member of the Liberal National Party and an independent MP.

He also ran as a PUP candidate at the last federal election in the seat of Hinkler.

Mr Messenger said he was disappointed by the "personal attacks" on himself, but would continue to help Senator Lambie do her job.

"Jacqui's my boss, the people of Tasmania are her boss, [and] I'm here to help her," Mr Messenger told reporters in north-west Tasmania, as Senator Lambie watched on.

Senator Lambie said she did not realise Mr Messenger was still a member of the Palmer United Party, and it is better for him not to be.

She also fired back at Mr Palmer, saying he should speak directly with her if he has a problem.

"What I find insulting from Clive Palmer is now he's starting to go at staff," she said.

"If Clive wants to have a shot, let him have it at me.

"To go at staff - that's a whole new low for Clive Palmer."

Frictions within the party have boiled over in recent days, with Senator Lambie publicly criticising the party's strategy and refusing to endorse her colleagues' performance.

"One thing I won't do is lie to the Australian people and I'm not going to sit here and make my PUP senators or Clive Palmer look good when I'm not feeling that way about them," she told the ABC's 7.30 program on Wednesday night.

Space to play or pause, M to mute, left and right arrows to seek, up and down arrows for volume. Watch Duration: 7 minutes 57 seconds 7 m Watch Jacqui Lambie's 7.30 interview

"I won't do that... I just don't feel like they're helping me out.

"So if that means I've got to go it alone under the PUP flag then so be it, I'll just run it alone."

Senator Lambie is angry at the Federal Government's pay deal for Defence personnel, and at how the PUP has responded to it.

She vowed to vote against all government legislation until the pay offer is improved, something Mr Palmer said was not appropriate.

"We aren't prepared to give a blanket threat to vote against the Liberal Government for the next five years," Mr Palmer said.

"No such proposal has been considered in our party room.

"I am amazed why Jacqui Lambie is being such a drama queen when the Palmer United Party unanimously supports the ADF and opposes the Government's proposed pay structure.

"If what she says about being unhappy with her party's leadership is true, she should make a challenge.

"Otherwise get on with the job of representing the people who voted for her."

Lambie wishes she never joined PUP

Senator Lambie said she does not intend to challenge for the leadership, but remained critical of how Mr Palmer was running the party.

Mr Messenger ran as a Palmer United Party candidate at the last federal election in the seat of Hinkler. ( ABC Open: Brad Marsellos )

"I have no doubts I could be a better leader than Clive Palmer," she said.

"I'm just not sure that being a leader of Palmer United Party would be in my best interest."

Senator Lambie said she now regrets her decision to join the party, and wishes she had campaigned as an independent candidate at the last election.

She said she "doesn't have a choice" about staying, because she made a verbal agreement to remain a member of the team.

However, if Mr Palmer asked her to leave the party, she would.

"I'm actually surprised Clive hasn't asked me to leave - let's be honest," she said.

Space to play or pause, M to mute, left and right arrows to seek, up and down arrows for volume. Listen Duration: 4 minutes 14 seconds 4 m 14 s Tasmanians rate Jacqui Lambie's performance Download 7.8 MB

Crossbencher Senator David Leyonhjelm said he and Family First Senator Bob Day have discussed increasing their power bloc to three.

"If she [Senator Lambie] is looking for a friend, Bob and I are here to help her," Senator Leyonhjelm said.

The Government needs six crossbenchers to pass legislation without Labor and the Greens.

Earlier on Thursday the party's Senate leader, Glenn Lazarus, said he was disappointed with Senator Lambie's criticism of her colleagues.

Asked if he thought she would leave the party, he replied: "I don't know, to be honest."

"She hasn't made any contact with me.

"Until we speak and sort out what's going on, there's not much I can do at this present time."