There is no evidence that Mr. Biden, as vice president, intentionally tried to help his son in his dealings with Ukraine, but Hunter Biden’s business career has sporadically drawn attention from political rivals and the media for more than a decade. Associates say Mr. Biden is infuriated by Mr. Trump’s conduct and astonished by the president’s willingness to dismiss some of the most basic ethical lines in American politics.

In Wilmington, Del., Tuesday, Mr. Biden called the president’s actions an “abuse of power’’ that undermined national security, and said he would support impeachment if Mr. Trump did not provide information related to congressional investigations.

In some respects, Mr. Trump’s conduct has reinforced the central message of Mr. Biden’s appeal to Democratic voters: that the country is threatened by an out-of-control presidency, and that ousting Mr. Trump is more important than any other policy goal Democrats might share.

At fund-raisers and other events, Democrats who have spoken with Mr. Biden in recent days say he has largely appeared measured, greeting old friends in his typical effusive style and keeping the focus on his concerns with Mr. Trump’s foreign policy, rather than dwelling on the challenges the moment raises for his family.

Still unclear in this moment of crisis is what conclusions Democratic voters might make about Mr. Biden as an opponent for Mr. Trump, and how a drive for impeachment in Congress might shape the Democratic primary campaign.

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Former Gov. Tom Vilsack of Iowa, who saw Mr. Biden at a fund-raiser in Iowa on Saturday, said he believed that Mr. Trump’s dangerous conduct was at the forefront of Mr. Biden’s mind.