An alleged Queens prostitute linked to the overdose deaths of three men — including Cipriani Dolci head chef Andrea Zamperoni — has been charged with providing the fatal dose for another man.

Angelina Barini, 41, was arraigned Thursday on federal charges of distributing and possessing with intent to distribute fentanyl, methamphetamine and cocaine that resulted in the deadly overdose of a 60-year-old Queens man on Aug. 5.

Barini, who has been behind bars since late August, appeared in Brooklyn federal court wearing dark blue jail scrubs — her face streaked with tears — to enter a not guilty plea.

Her alleged victim — who wasn’t named — was a retired postal worker, a source with knowledge of the investigation said. He died at his home in College Point.

“The defendant Angelina Barini now stands charged with dispensing deadly doses of narcotic drugs to two victims,” US Attorney Richard Donoghue said in a release.

“Every life lost to the opioid crisis is of the utmost importance to this Office and our law enforcement partners, and we will work relentlessly to uncover the evidence proving who is responsible for each opioid death.”

The new charge comes less than two weeks after she pleaded not guilty to causing the July 11 overdose death of another man at a Woodside motel.

While the John Doe victim has not been identified in court papers, the Office of the Chief Medical Examiner reported that Jean-Alessandro Silvero died on July 11 from fentanyl intoxication at a Woodside motel.

Barini has been hit with drug-distribution charges in connection with ­Zamperoni’s death.

According to court papers, police arrested Barini Aug. 21 in the chef’s death after she answered the door at room 15 at the Kamway Lodge in Elmhurst — the same room where Zamperoni’s body was found.

He had allegedly been stuffed in a garbage can.

“In the corner of the room, law enforcement authorities noticed what appeared to be a garbage can with bed linens stuffed inside and what appeared to be a bare, human foot sticking out of the bed linens,” court docs say.

Court papers in that case said Barini admitted she was a prostitute and had given the chef liquid ecstasy — and was unable to wake him when she found him bleeding from the nose and mouth.

The medical examiner said that alcohol, cocaine and the sedative GHB were found in his system.

The promising 33-year-old chef had been missing for five days when he was found at the $89-a-night rooming house.

Zamperoni had worked at the Grand Central Terminal eatery for more than a year, after working for more than a decade at a Cipriani restaurant in London.

Prosecutors also have linked Barini to another John Doe, who was found dead on July 4 at a hotel near La Guardia Airport.