Donald Trump at a rally in Pennsylvania. Jeff Swensen/Getty Images Donald Trump unleashed on House Speaker Paul Ryan Tuesday morning, one day after the Wisconsin Republican announced he would no longer defend or campaign for the GOP presidential nominee.

In a series of tweets, the real-estate mogul praised his own debate performance — citing unscientific polls to do so — and simultaneously slammed the speaker's leadership.

"Our very weak and ineffective leader, Paul Ryan, had a bad conference call where his members went wild at his disloyalty," Trump wrote.

"Despite winning the second debate in a landslide (every poll), it is hard to do well when Paul Ryan and others give zero support!" Trump said in an earlier tweet.

The only scientific poll conducted in the immediate aftermath of the second debate on Sunday found that Hillary Clinton walked away with a decisive victory.

The barbs from Trump came less than 24 hours after he tweeted that Ryan should stop spending his time criticizing the nominee.

"Paul Ryan should spend more time on balancing the budget, jobs and illegal immigration and not waste his time on fighting Republican nominee," Trump wrote on Monday.

Ryan's decision not to defend Trump followed the release on Friday of a video from 2005 that showed Trump bragging in private about kissing, grabbing, and forcing himself onto women without their permission. Sens. John McCain, Kelly Ayotte, John Thune, and Rob Portman are among a slew of prominent Republican officials who unendorsed the nominee after revelations about Trump's 2005 comments.

For his part, Ryan is still endorsing Trump, though the two earlier this year flirted with the possibility of not endorsing each other.

A spokesperson for Ryan told Business Insider on Tuesday that the speaker "is focusing the next month on defeating Democrats, and all Republicans running for office should probably do the same."