We thank I. Matos for assistance with live-imaging set-up; F. García-Quiroz for assistance with TEWL experiments; L. Polak, L. Hidalgo, and M. Sribour for animal assistance; R. Eisenman for Mycn floxed mice; A. Asare for validating Gulp1 shRNAs; L. Johnston, E. Bach, M. Simunovic, and all Fuchs laboratory members, but especially M. Laurin, A. Sendoel, S. Baksh, S. Gur-Cohen, S. Liu and K. Stewart for discussion and/or comments on the manuscript; Rockefeller University (RU) Bio-Imaging Resource Center for use of microscopes; RU Comparative Bioscience Center (AAALAC-accredited) for care of mice in accordance with National Institutes of Health (NIH) guidelines; and RU Flow Cytometry Resource Center for assistance with FACS. S.J.E. was supported by fellowships from the Human Frontiers Science Program (LT000907/2015) and RU Women & Science. N.C.G. holds a Burroughs Wellcome Fund Postdoctoral Enrichment Program Award and an NIH Postdoctoral Ruth L. Kirschstein National Research Service Award (F32CA221353). A.F.M. was supported by an Arnold O. Beckman Postdoctoral Fellowship. Y.G. is supported by a NIAMS 1K01AR072132-01A1 Career Development Award and an Irene Diamond Fund/AFAR Postdoctoral Transition Award in Aging. E.F. is an HHMI Investigator. This research was supported by the NIH (R01-AR27883 to E.F.).

Reviewer information

Nature thanks Bruce Edgar, Emma Rawlins and the other anonymous reviewer(s) for their contribution to the peer review of this work.