As enemy healers go Ana is a scary one for Probius. Her sleep dart can cause a lot of problems if you get caught off-guard and out of position.

However, Ana is a low mobility hero (with the option to lower her mobility even further) meaning that if she is out of position Probius can punish her incredibly hard, as well as being able to deny her movement with his zone control.

Things to look out for:

Sleep dart, if you get hit with the dart it can often lead to your death

Nano-boost, when boosted many hero’s can become a ‘1-shot’ threat to Probius

Eye of Horus, this should really not be too much of a threat considering your move speed and tiny hitbox, but it is worth mentioning as Ana can use it to effectively cancel your Z (worker rush) or to catch you off-guard and land a kill from safety if you are low

Try to Exploit:

When Ana hits D and enters her slow moving stance she cannot escape a Warp rift in time to avoid its damage if you place it underneath her

Ana is vulnerable if zoned away from her teams with cannons and rifts

Ana has limited self healing (unless she can stack shrike on a member of your team) chip her down

Early Game:

In the early game try and position your cannons on the flank of your opponents, back from the minion wave. This means if they try and push up to be aggressive your cannon will target their backline.

Early in the game everyone should be mostly focused on soak, you and Ana should not have that much impact on each other at this stage.

Mid Game:

Try to make It hard for Ana to get into position and support her team, cannons and rifts are excellent ways to deter her from entering a zone as she has no way to avoid or deal with them.

This means that for teamfights and objective spawns pay attention to Ana’s rotation path and try to think about where she will want to set-up during the fight.

I would not recommend necessarily holding rifts back to try and punish Ana once the fight starts, this is because she will be far back and you moving in to punish her once the opposing team is in position is likely to be a suicide run.

Late Game:

In the late game you must always be aware of the possibility of a nano, or sleep dart. If you have not seen Sleep dart used recently be wary of stepping up!

Otherwise keep up the mid-game plan of making it hard for Ana to effectively enter the fight and support her team. This can result is fights ending in your teams favour before she even arrives; in many cases this can win you the game.

Conclusion:

Ana is one of the strongest healer picks vs a Probius. She has strong burst healing, that she can dispense from a safe distance and low enough cooldowns (2 seconds on healing dart) to keep pace with Probius’s rifts.

There is not much Probius can do to interact with Ana and disrupt her game plan if she plays well. Though the inverse is also true, outside of her ultimates.