1. Hönisch, B. et al. The geological record of ocean acidification. Science 335, 1058–1063 (2012).

2. Lüthi, D. et al. High-resolution carbon dioxide concentration record 650,000–800,000 years before present. Nature 453, 379–382 (2008).

3. Riebesell, U. & Gattuso, J.-P. Lessons learned from ocean acidification research. Nat. Clim. Change 5, 12–14 (2015).

4. Dixson, D. L., Munday, P. L. & Jones, G. P. Ocean acidification disrupts the innate ability of fish to detect predator olfactory cues. Ecol. Lett. 13, 68–75 (2010).

5. Munday, P. L. et al. Replenishment of fish populations is threatened by ocean acidification. Proc. Natl Acad. Sci. USA 107, 12930–12934 (2010).

6. Ishimatsu, A., Hayashi, M., Lee, K.-S., Kikkawa, T. & Kita, J. Physiological effects on fishes in a high-CO 2 world. J. Geophys. Res. Oceans 110, C09S09 (2005).

7. Heuer, R. M. & Grosell, M. Physiological impacts of elevated carbon dioxide and ocean acidification on fish. Am. J. Physiol. Regul. Integr. Comp. Physiol. 307, R1061–R1084 (2014).

8. Melzner, F. et al. Physiological basis for high CO 2 tolerance in marine ectothermic animals: pre-adaptation through lifestyle and ontogeny? Biogeosciences 6, 2313–2331 (2009).

9. Munday, P. L. et al. Ocean acidification impairs olfactory discrimination and homing ability of a marine fish. Proc. Natl Acad. Sci. USA 106, 1848–1852 (2009).

10. Shaw, E. C., McNeil, B. I. & Tilbrook, B. Impacts of ocean acidification in naturally variable coral reef flat ecosystems. J. Geophys. Res. Oceans 117, C03038 (2012).

11. Clements, J. C. & Hunt, H. L. Marine animal behaviour in a high CO 2 ocean. Mar. Ecol. Prog. Ser. 536, 259–279 (2015).

12. McNeil, B. I. & Sasse, T. P. Future ocean hypercapnia driven by anthropogenic amplification of the natural CO 2 cycle. Nature 529, 383–386 (2016).

13. Leis, J. M. Paradigm lost: ocean acidification will overturn the concept of larval-fish biophysical dispersal. Front. Mar. Sci. 5, 47 (2018).

14. Bignami, S., Sponaugle, S. & Cowen, R. K. Response to ocean acidification in larvae of a large tropical marine fish, Rachycentron canadum. Glob. Change Biol. 19, 996–1006 (2013).

15. Sundin, J., Amcoff, M., Mateos-González, F., Raby, G. D. & Clark, T. D. Long-term acclimation to near-future ocean acidification has negligible effects on energetic attributes in a juvenile coral reef fish. Oecologia 190, 689–702 (2019).

16. Munday, P. L., Cheal, A. J., Dixson, D. L., Rummer, J. L. & Fabricius, K. E. Behavioural impairment in reef fishes caused by ocean acidification at CO 2 seeps. Nat. Clim. Change 4, 487–492 (2014).

17. Nilsson, G. E. et al. Near-future carbon dioxide levels alter fish behaviour by interfering with neurotransmitter function. Nat. Clim. Change 2, 201–204 (2012).

18. Button, K. S. et al. Power failure: why small sample size undermines the reliability of neuroscience. Nat. Rev. Neurosci. 14, 365–376 (2013).

19. Baker, M. 1,500 scientists lift the lid on reproducibility. Nature 533, 452–454 (2016).

20. Eisenstein, M. Public health: an injection of trust. Nature 507, S17–S19 (2014).

21. Browman, H. I. Applying organized scepticism to ocean acidification research. ICES J. Mar. Sci. 73, 529–536 (2016).

22. Parker, T. H. et al. Transparency in ecology and evolution: real problems, real solutions. Trends Ecol. Evol. 31, 711–719 (2016).

23. Clark, T. D. Science, lies and video-taped experiments. Nature 542, 139 (2017).

24. Clark, T. D. et al. Scientific misconduct: the elephant in the lab. A response to Parker et al. Trends Ecol. Evol. 31, 899–900 (2016).

25. Welch, M. J., Watson, S.-A., Welsh, J. Q., McCormick, M. I. & Munday, P. L. Effects of elevated CO 2 on fish behaviour undiminished by transgenerational acclimation. Nat. Clim. Change 4, 1086–1089 (2014).

26. Ferrari, M. C. O. et al. Effects of ocean acidification on visual risk assessment in coral reef fishes. Funct. Ecol. 26, 553–558 (2012).

27. Munday, P. L. et al. Elevated CO 2 affects the behavior of an ecologically and economically important coral reef fish. Mar. Biol. 160, 2137–2144 (2013).

28. Vallortigara, G. & Rogers, L. J. Survival with an asymmetrical brain: advantages and disadvantages of cerebral lateralization. Behav. Brain Sci. 28, 575–589 (2005).

29. Chung, W.-S., Marshall, N. J., Watson, S.-A., Munday, P. L. & Nilsson, G. E. Ocean acidification slows retinal function in a damselfish through interference with GABA A receptors. J. Exp. Biol. 217, 323–326 (2014).

30. Pecl, G. T. et al. Biodiversity redistribution under climate change: impacts on ecosystems and human well-being. Science 355, eaai9214 (2017).

31. Gould, A. L., Harii, S. & Dunlap, P. V. Cues from the reef: olfactory preferences of a symbiotically luminous cardinalfish. Coral Reefs 34, 673–677 (2015).

32. Dixson, D. L., Abrego, D. & Hay, M. E. Chemically mediated behavior of recruiting corals and fishes: a tipping point that may limit reef recovery. Science 345, 892–897 (2014).

33. Riebesell, U., Fabry, V. J., Hansson, L. & Gattuso, J.-P. Guide to Best Practices for Ocean Acidification Research and Data Reporting (Publications Office of the European Union Luxembourg, 2010).

34. Moran, D. The importance of accurate CO 2 dosing and measurement in ocean acidification studies. J. Exp. Biol. 217, 1827–1828 (2014).

35. Cornwall, C. E. & Hurd, C. L. Experimental design in ocean acidification research: problems and solutions. ICES J. Mar. Sci. 73, 572–581 (2016).

36. Dickson, A. G. Standard potential of the reaction: AgCl(s) + 1 2 H 2 (g) = Ag(s) + HCl(aq), and the standard acidity constant of the ion HSO 4 − in synthetic sea water from 273.15 to 318.15 K. J. Chem. Thermodyn. 22, 113–127 (1990).

37. Lueker, T. J., Dickson, A. G. & Keeling, C. D. Ocean pCO 2 calculated from dissolved inorganic carbon, alkalinity, and equations for K 1 and K 2 : validation based on laboratory measurements of CO 2 in gas and seawater at equilibrium. Mar. Chem. 70, 105–119 (2000).

38. Jutfelt, F., Bresolin de Souza, K., Vuylsteke, A. & Sturve, J. Behavioural disturbances in a temperate fish exposed to sustained high-CO 2 levels. PLoS One 8, e65825 (2013).

39. Ishimatsu, A., Hayashi, M. & Kikkawa, T. Fishes in high-CO 2 , acidified oceans. Mar. Ecol. Prog. Ser. 373, 295–302 (2008).

40. Ou, M. et al. Responses of pink salmon to CO 2 -induced aquatic acidification. Nat. Clim. Change 5, 950–955 (2015).

41. Munday, P. L. et al. Selective mortality associated with variation in CO 2 tolerance in a marine fish. Ocean Acidif. 1, 1–5 (2012).

42. Green, L. & Jutfelt, F. Elevated carbon dioxide alters the plasma composition and behaviour of a shark. Biol. Lett. 10, 20140538 (2014).

43. Sundin, J. et al. Long-term exposure to elevated carbon dioxide does not alter activity levels of a coral reef fish in response to predator chemical cues. Behav. Ecol. Sociobiol. 71, 108 (2017).

44. Doherty, P. J. Light-traps: selective but useful devices for quantifying the distributions and abundances of larval fishes. Bull. Mar. Sci. 41, 423–431 (1987).

45. Jutfelt, F., Sundin, J., Raby, G. D., Krang, A.-S. & Clark, T. D. Two-current choice flumes for testing avoidance and preference in aquatic animals. Methods Ecol. Evol. 8, 379–390 (2017).

46. Atema, J., Kingsford, M. J. & Gerlach, G. Larval reef fish could use odour for detection, retention and orientation to reefs. Mar. Ecol. Prog. Ser. 241, 151–160 (2002).

47. Bisazza, A., Facchin, L., Pignatti, R. & Vallortigara, G. Lateralization of detour behaviour in poeciliid fish: the effect of species, gender and sexual motivation. Behav. Brain Res. 91, 157–164 (1998).

48. Bisazza, A., Pignatti, R. & Vallortigara, G. Detour tests reveal task- and stimulus-specific behavioral lateralization in mosquitofish (Gambusia holbrooki). Behav. Brain Res. 89, 237–242 (1997).

49. Nakagawa, S. A farewell to Bonferroni: the problems of low statistical power and publication bias. Behav. Ecol. 15, 1044–1045 (2004).

50. Zuur, A. F., Ieno, E. N., Walker, N. J., Saveliev, A. A. & Smith, G. M. Mixed Effects Models and Extensions in Ecology with R (Springer, 2009).

51. Simonsohn, U. Just post it: the lesson from two cases of fabricated data detected by statistics alone. Psychol. Sci. 24, 1875–1888 (2013).

52. Roche, D. et al. Replication alert: behavioural lateralisation in a detour test is not repeatable in fishes. Preprint at EcoEvoRxiv https://doi.org/10.32942/osf.io/6kcwa (2019).

53. Domenici, P., Allan, B., McCormick, M. I. & Munday, P. L. Elevated carbon dioxide affects behavioural lateralization in a coral reef fish. Biol. Lett. 8, 78–81 (2012).

54. Roche, D. G., Binning, S. A., Strong, L. E., Davies, J. N. & Jennions, M. D. Increased behavioural lateralization in parasitized coral reef fish. Behav. Ecol. Sociobiol. 67, 1339–1344 (2013).

55. Roche, D. G., Kruuk, L. E. B., Lanfear, R. & Binning, S. A. Public data archiving in ecology and evolution: how well are we doing? PLoS Biol. 13, e1002295 (2015).