The Upright Citizens Brigade, which has nurtured the careers of many beloved improv and sketch comedians, is upgrading the real estate for one of its theaters.

It will be leaving the Chelsea location it has occupied since 2003 by the end of November and moving closer to the heart of the theater district at 555 West 42nd Street, the former space of the Pearl Theater Company, The Wall Street Journal reported on Monday. The move was made in part to comply with the Americans With Disabilities Act.

“We have a very diverse group of students, performers and patrons and we want to be able to serve all of them,” Shannon O’Neill, the artistic director of Upright Citizens Brigade Theater New York, told The Journal. “Right now, the Chelsea theater doesn’t allow that.”

The Chelsea location, the most famous out of U.C.B.’s four theaters, frequently has lines around the block for its shows and has become a New York City institution. The theater was founded by Amy Poehler, Ian Roberts, Matt Walsh and Matt Besser at another Chelsea location during the run of their Comedy Central sketch show, “Upright Citizens Brigade,” which ran from 1998 to 2000.