Parents who refuse to vaccinate their children will not be able to enrol them in childcare or kindergarten under new laws being pushed by the Victorian government.

The tightening of the state's No Jab No Play laws will see anti-vax parents forced to provide proof of serious medical conditions if they want to enrol unvaccinated children in childcare centres, the Herald Sun reports .

At the moment families are able to get around the Victorian laws with notes from doctors, but the loophole is set to be closed up following the discovery of a network of anti-vax GPs.

The stricter legislation is expected to be introduced by the Andrews Government today.

The legislation will rule out letters from GPs and immunisation providers as proof of vaccinations, instead insisting parents to provide official statements from the Australian Immunisation Register (AIR).

Parents of unvaccinated children will need to provide AIR documents explaining the reasons for the decision.

All parents will be required to provide regular vaccination updates.

The legislation also calls for regular audits of child care centres to make sure everyone is up to date with their vaccinations.

Health Minister Jill Hennessy said the Andrews Government is making “no apologies for cracking down on rogue doctors who don’t put the health of our community first”.

“The science is crystal clear — vaccinations are safe and save lives,” she said.

“People who tout misinformation about immunisation are a menace and put the health and safety of children at risk.

“That’s why we want as many children as possible immunised and protected from life-threatening illnesses.”