We thank the Yano/Yanchandra Laboratory for T. elongatus membranes; E. A. Montabana for cryo-EM training of T.G.L. and advice on sample preparation; D. Toso for assistance during data collection; P. Tobias for technical support; T. H. D. Nyugen and B. J. Greber for advice on model-building; and L. M. Oltrogge, A. Flamholz, J. J. Demarais, D. Serwas and N. Fisher for comments on the manuscript. We thank Donner Laboratories at Lawrence Berkeley National Laboratory and the Bay Area Cryo-EM facility at the University of California, Berkeley for microscope screening and data collection access, respectively. We thank the McGill Pharmacology SPR/MS facility and the Canadian Fund for Innovation for infrastructure support. T.G.L. was supported by a National Science Foundation Graduate Research Fellowship and Molecular Basis of Cell Function National Institutes of Health pre-doctoral training grant (NIGMS project 5T32GM007232-38). A.N.B. was supported by a Healthy Brain for Healthy Lives studentship and J.-F.T. was supported by a Tier 2 Canada Research Chair. D.F.S. and K.M.D. were supported by the US Department of Energy grants DE-SC00016240 and DE-AC02-O5CH11231, respectively.

Reviewer information

Nature thanks R.Cogdell, A. Leitner and the other anonymous reviewer(s) for their contribution to the peer review of this work.