Yes, and no...

Donald Duck's first love interest was Donna Duck, first appearing in the Don Donald 1937 short film.



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However, this is where things get confusing. Whilst she has appeared in comics alongside Daisy, Disney states that they are the same character.



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I can't actually find a copy online to get the quote from it (will keep looking) but apparently Disney Dossiers: Files of Characters From the Walt Disney Studios refers to them as the same character. In the Walt Disney Cartoon Classics: Starring Donald and Daisy VHS the Don Donald short was re-released and it mentions it stars Donald and Daisy. See the still below and the video after it.



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As such it would certainly appear that the characters were combined, even though they clearly aren't the same character.

...But also yes

We also have Reginella, the queen of an alien civilisation and the pair do love each other but are kept apart. However, they meet a few times and appear to be in love.