Three people died in a crash near Guelph on Saturday night, Ontario Provincial Police say, the second triple fatal on the same day during the Thanksgiving holiday weekend.

Police say the crash near Guelph involved a pickup truck and a sedan on Wellington Road 44, south of Highway 7, outside Rockwood, Ont., at about 8:30 p.m. ET.

All three people were in the same vehicle and from Fergus.

A male passenger, 35, was pronounced dead at the scene. Another male passenger, 17, and the female driver, 45, were taken to hospital with life-threatening injuries. Police said those two victims later died of their injuries.

The driver of the second vehicle — a 50-year-old man, from Barrie — was not injured, police said.

Firefighters and police stand at the scene of a triple fatal crash outside of Rockwood on Saturday evening. (David Ritchie/CBC) Police did not give the direction each vehicle was travelling or how the crash occurred.

Police are investigating the cause and appealing for witnesses to contact them. Next of kin are being notified. The names of the victims have not been released.

Crash follows triple fatal near Port Perry

Earlier Saturday, provincial police said three people died near Port Perry, Ont. when an SUV and a car collided head-on.

In that crash, the SUV travelling northbound crossed into the southbound lanes of Highway 7/12 at 4th Line in Scugog township, about 80 kilometres northeast of Toronto, and struck a southbound car with four people inside. Police were called to the scene at 1:15 a.m.

Ontario Provincial Police say a male driver and two female passengers in a car were pronounced dead at the scene of a highway accident near Port Perry, Ont., early Saturday. A male driver in an SUV and another female passenger of the car were injured. (Colin Williamson/CBC)

Three people in the car — a man, 59, and two women, 55 and 44 — died at the scene. Another 41-year-old woman who was a passenger in the car suffered critical injuries. The 68-year-old man driving the SUV suffered serious injuries.

At the start of the Thanksgiving long weekend, the OPP announced it was taking part in Operation Impact, a national weekend campaign by police forces to make roads safer.

The campaign is focused on the four leading causes of road fatalities: aggressive driving, impaired driving, inattentive driving and failure to use a seatbelt.