A growing majority of Australians now oppose the construction of Adani's huge Carmichael coal mine, while environmental groups are ramping up pressure on Bill Shorten and federal Labor to rule out support for the project.

A poll of 3312 people, conducted by pollsters ReachTEL on January 25 and commissioned by the Stop Adani Alliance, found 65.1 per cent of Australians opposed or strongly opposed Indian mining company Adani building the new coal mine in Queensland.

The figure represents a 13.2 per cent rise - from 51.9 per cent - in opposition to the project compared to March 2017. Significantly, the latest poll found an outright majority of Nationals (55.3 per cent), One Nation (52.9 per cent), Labor (75.6 per cent) and Greens (94.2 per cent) voters all oppose the mine.

More Liberal voters (43.2 per cent) said they opposed or strongly opposed the project compared to 34.7 per cent who said they supported or strongly supported it.

The findings come a day after Mr Shorten told the National Press Club the project had to stack up commercially and environmentally for federal Labor to support it, and that more needed to be done to protect the Great Barrier Reef, which environmental groups warn will be negatively impacted by the project.