The ongoing controversy over publication of two studies involving the transmission in ferrets of H5N1 (H5) subtype influenza viruses and the recommendations of the National Science Advisory Board for Biosecurity to redact key details in the manuscripts call for an examination of relevant scientific facts. In addition, there are calls in the media to destroy the viruses, curtail future research in this area, and protect the public from such “frightening” research efforts. Fear needs to be put to rest with solid science and not speculation.

From a broader perspective, publishing relevant methods and data related to important experimental findings is fundamental to progress in science. Enabling others to repeat published data makes it possible to expand on what is known; the cycle of publication followed by experimental replication and advancement has resulted in the majority of our scientific and medical achievements. With specific reference to influenza viruses, the free flow of data has enabled the timely development of vaccines and other medications for seasonal and pandemic disease, and has, without question, saved millions of lives.

With respect to the specific papers by Fouchier and Kawaoka, it would be important for other scientists to replicate portions of these works to test new vaccines/therapeutic agents and for continued studies on the molecular aspects of influenza transmission, a topic that is extremely important yet relatively poorly understood ( 25 ⇓ – 27 ).

It is known that H5 viruses have been circulating in poultry for at least 50 years (probably much longer) and that they cause natural infection and even transmit between some mammals such as pigs, dogs, and cats ( 21 ⇓ ⇓ – 24 ) ( Figs. 1 and 2 ). Despite regular human contact with animal reservoirs and the virus’ propensity to mutate, strains able to cause sustained disease in humans have not emerged. In fact, in humans, only influenza viruses of the subtypes H1, H2, and H3 have circulated during the past 100 years, suggesting that other subtypes may not easily become significant human pathogens.

The ferret system is an important model for the study of influenza transmission, virulence, and immunity ( 7 ⇓ ⇓ ⇓ – 11 ). Generally, the ferret model provides a more accurate reflection of how influenza viruses affect humans compared with the mouse model (another common system) ( 12 ). Ferrets are quite susceptible to infection with influenza viruses. However, it is not clear that all virus strains that replicate in and transmit between ferrets necessarily do so in humans ( 13 , 14 ). Ferrets are also more likely than humans to have disseminated, multiorgan influenza disease including neurologic sequelae resulting from virus replication in the brain ( 15 ⇓ ⇓ – 18 ). Thus, the mutations identified in the studies by Fouchier and Kawaoka could cause the viruses to be more transmissible between humans, but this is simply unknowable from the available data. The viruses may well be more adapted to ferrets, but not more adapted to other mammals; one cannot directly extrapolate from the data to make predictions about humans. In fact, passage of viruses in a different host is the most frequently used strategy to reduce viral virulence in humans. Many live, attenuated virus vaccines have been generated by that approach, including those for poliovirus and yellow fever virus. Further experiments in other mammalian systems would enhance our understanding of the potential for the H5 viruses to transmit between nonferret mammals and/or cause disease in these systems ( 19 ⇓ – 21 ).

The experiments demonstrate the important finding that aerosol transmission between ferrets can be determined by a relatively small number of mutations that do not significantly change virus virulence. Thus, the need for continued surveillance efforts around H5 viruses is clearly reinforced by these works, as is the importance of continuing to develop H5 vaccines and therapeutics ( 6 ).

The studies use the age-old method of virus adaptation by repeated passage in cells or in naive animal hosts. H5 viruses (WT or with specific mutations introduced that are known to confer tropism to mammalian tissue) were passaged in ferrets, a well established animal model for the study of influenza transmission ( 1 ⇓ ⇓ ⇓ – 5 ). Ultimately, both studies determined that, indeed, H5 viruses could acquire aerosol transmissibility while maintaining virulence in ferrets. *

The two studies discussed here are by Ron Fouchier at Erasmus Medical Center in Rotterdam and Yoshihiro Kawaoka at the University of Wisconsin (not yet published by Science and Nature, respectively). Both senior authors are experienced virologists who have worked for years with highly pathogenic avian influenza viruses. Their respective studies differ in many ways, but both were designed to answer one question of whether H5 influenza viruses can achieve sustainable aerosol transmission in ferrets.

Fear

With the understanding that all parties involved have worthy intentions, it is difficult to ignore some of the incongruities that overshadow discussions related to H5 viruses and the publication of these two manuscripts. Examination of the topics discussed below may relieve some of the fear that is fueling the current debate.

Is the Case Fatality Rate for Human H5 Infections Truly Greater than 50%? At the heart of this controversy is the notion that the case fatality rate for human H5 infections is in the range of 50% to 80%. This rate is derived from the list of H5 cases that have been “confirmed” under World Health Organization (WHO) guidelines; the list tallies 573 cases in 15 countries (as of December 15, 2011), with nearly 60% of total cases resulting in death (28). The frequency and certainty with which this staggering fatality rate is reported is troubling when one considers how the numbers are generated; in order for a case to be confirmed by WHO, a person must have an acute, febrile respiratory illness (temperature >38 °C/100.3 °F) with known H5 exposure in the 7 days preceding symptom onset and have molecular confirmation of H5 infection by a WHO-approved laboratory (e.g., virus isolation, PCR, serology) (29). This definition does not allow for asymptomatic infections and essentially requires that a person actively seek medical help at a hospital that is equipped to draw samples and ship them to an approved laboratory. Given that rural populations in developing countries are most commonly affected by H5 viruses, it seems unlikely that even a small fraction of the total number of infected cases has been accounted for under the WHO surveillance system. Also, the fatal cases that have been reported are most likely caused by mega-doses of H5 virus inhaled by the patients, who are living in very close contact with infected poultry. Transmission involving small doses of virus (as observed under regular aerosol transmission conditions) may not result in overt disease. Of the 10 largest studies of which we are aware (N ≥ 500) (30⇓⇓⇓⇓⇓⇓⇓⇓–39), which document seroevidence in humans for H5 infections, two studies report no seropositivity and the other eight report rates ranging from 0.2% to 5.6%; the studies are mostly conducted in rural areas where H5 infections have been previously documented (30⇓⇓⇓⇓⇓⇓⇓⇓–39). Even if only a low percentage of the rural population is asymptomatically/subclinically infected, the case fatality rate that is offered by the WHO—and that is driving this controversy—is likely orders of magnitude too high. This suggests that more work should be done to determine an accurate case fatality rate for H5 infections, which takes into account mild and asymptomatic cases.

Why These Papers, Why Now? The type of work performed in the studies under evaluation is quite common in virology because it is a useful way to study molecular determinants of virus adaptation, pathogenesis, and transmission. The reasoning behind the selection by the National Science Advisory Board for Biosecurity of these specific papers for redaction/censorship seems somewhat arbitrary relative to what has previously been published in the field of influenza. In 2005, the complete sequences for the 1918 pandemic influenza virus were published in Nature and methods describing the rescue of the 1918 virus were published in Science (40, 41). In 2006, both Science and Nature published reports of specific mutations that enable the H5 viral hemagglutinin to bind human, rather than avian, tissues (42, 43). In 2012, a report from the Centers for Disease Control and Prevention that bears striking resemblance, in principle, to the works by Fouchier and Kawaoka was already published in Virology; it describes mutations in an H5N1 virus that confer airborne transmissibility between ferrets (44). Studies have also been published recently that describe mutations enabling other potential pandemic strains to transmit between ferrets (45, 46) or that make currently circulating virus strains more virulent (47). These are a small fraction of studies published during the past decade that could have been considered controversial (some were controversial at the time, but all were published in full). We know from studies like these that, for example, the 2009 pandemic vaccine protects against the 1918 pandemic virus and thus the 1918 strain is not a pandemic threat at this time (48), nor would it be of any advantage for a bioterrorist to lay his/her hands on it. Other striking examples of achievements based on the free exchange of potentially controversial information are the elimination of SARS corona virus in only 6 months and the fact that the dangers of HIV have been dramatically reduced by the development of effective antiviral treatments. None of this would have been possible without the uncensored exchange of scientific data.