Revolution OS is a documentary which traces the history of GNU, Linux, and the open source and free software movements. It features several interviews with prominent hackers and entrepreneurs (and hackers-cum-entrepreneurs), including Richard Stallman, Michael Tiemann, Linus Torvalds, Larry Augustin, Eric S. Raymond, Bruce Perens, Frank Hecker and Brian Behlendorf.

The film begins in medias res with an IPO, and then sets the historical stage by showing the beginnings of software development back in the day when software was shared on paper tape for the price of the paper itself. It then segues to Bill Gates's Open Letter to Hobbyists in which he asks Computer Hobbyists to not share, but to buy software. (This letter was written by Gates when Microsoft was still based in Arizona and spelled "Micro-Soft".)

Richard Stallman then explains how and why he left the MIT Lab for Artificial Intelligence in order to devote his life to the development of free software, as well as how he started with the GNU project. Linus Torvalds is interviewed on his development of the Linux kernel as well as on the GNU/Linux naming controversy and Linux's further evolution, including its commercialization. Richard Stallman remarks on some of the ideological aspects of open source vis-á-vis Communism and capitalism and well as on several aspects of the development of GNU/Linux.

Michael Tiemann (interviewed in a desert) tells how he met Stallman and got an early version of Stallman's GCC and founded Cygnus Solutions. Larry Augustin tells how he combined the resulting GNU software and a normal PC to create a UNIX-like Workstation which cost one third the price of a workstation by Sun Microsystems even though it was three times as powerful.

His narrative includes his early dealings with venture capitalists, the eventual capitalization and commodification of Linux for his own company, VA Linux, and ends with its IPO. Frank Hecker of Netscape tells how Netscape executives released the source code for Netscape's browser, one of the signal events which made Open Source a force to be reckoned with by business executives, the mainstream media, and the public at large.