"Enzyme That Makes You Cry–Crystal Structure of Lachrymatory Factor Synthase from Allium cepa"

ACS Chemical Biology

Adding onions to a recipe can make a meal taste rich and savory, but cutting up the onion can be brutal. Onions release a compound called lachrymatory factor (LF), which makes the eyes sting and water. Scientists know that a certain enzyme causes this irritating compound to form but precisely how it helps LF form in the onion remained an open question. Now, one group reports in ACS Chemical Biology that they have the answer.

According to the National Onion Association, the average American consumes 20 pounds of onions each year. When an onion is cut, it has a natural defense mechanism that springs into action, producing LF. This kind of compound is rare — only four known natural types exist. An enzyme in the onion known as lachrymatory factor synthase (LFS) spurs production of LF in the onion. “Tearless” onions, sold exclusively in Japan for a hefty price, don’t make LFS so they also don’t produce the irritant LF. But scientists have been at a loss to explain exactly how LFS helps LF form. That’s because it is extremely reactive, and LF evaporates or breaks down easily. Marcin Golczak and colleagues wanted to take a different approach to solve this mystery once and for all.

The team determined the crystal structure of LFS and analyzed it. With the crystal structure, they could finally see the architecture of the enzyme as a whole and its active site as it bound to another compound. By combining this information with known information about similar proteins, the group developed a detailed chemical mechanism that could explain the precise steps involved in LF synthesis—and hence, why people wind up crying when they chop an onion.

The authors acknowledge funding from the National Eye Institute of the National Institutes of Health , the U.S. Department of Veterans Affairs , and the American Society for Pharmacology and Experimental Therapeutics .

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