Introduction Homophobic behavior and a negative attitude toward homosexuals are prevalent among the population. Despite this, few researches have investigated the psychologic aspects associated with homophobia, as psychopathologic symptoms, the defensive system, and attachment styles.

Aim The aim of this study was to investigate the psychologic factors mentioned earlier and their correlation with homophobia.

Methods Five hundred fifty‐one university students recruited, aged 18–30, were asked to complete several psychometric evaluation.

Main Outcome Measures In particular, Homophobia Scale (HS) was used to assess homophobia levels, the Symptoms Check List Revised (SCL‐90‐R) for the identification of psychopathologic symptoms, the Defence Style Questionnaire (DSQ‐40) for the evaluation of defense mechanisms and the Relationship Questionnaire (RQ) for attachment styles.

Results After a regression analysis, we found a significant predictive value of psychoticism (β = 0.142; P = 0.04) and of immature defense mechanisms (β = 0.257; P < 0.0001) for homophobia, while neurotic defense mechanisms (β = −0.123; P = 0.02) and depressive symptoms (β = −0.152; P = 0.04) have an opposite role. Moreover, categorical constructs of the RQ revealed a significant difference between secure and fearful attachments styles in levels of homophobia (secure = 22.09 ± 17.22 vs. fearful = 31.07 ± 25.09; P < 0.05). Finally, a gender difference to HS scores and a significant influence of male sex was found (β = 0.213; P < 0.0001).