The December 9th, 2013, publication of the Curiosity team’s six papers in Science provides the basis for understanding a potentially habitable environment on ancient Mars. The search for habitable environments motivated building the rover, and to that end the Curiosity mission has accomplished its principal objective. This naturally leads to the questions of what’s next, and how we go about exploring for organic carbon?

To better understand where we’re coming from, it helps to break down these questions and analyze them separately. With future advocacy of missions to Mars so uncertain, and with difficult-to-grasp mission objectives located between "the search for water" (everyone got that) and "the search for life" (everyone wants NASA to get on with it), the "search for habitability" and the "search for carbon" are important intermediate steps. By focusing on them scientists can identify specific materials to study with more sophisticated future missions and instruments, or to select for sample return, or to be the target of life detection experiments.

Note: You can get access to all our papers here or here. The latter site also has the papers we published back in September. Science has a policy that allows us to post a "referrer link" to our home websites. This redirects the query to AAAS, where the paper can be downloaded without cost.

Habitability

Let’s start with "habitability." We reported the discovery of an ancient lake, and one that formed clay minerals. The presence of clays represents more benign environmental conditions than the acid sulfates found by Spirit and Opportunity. However, clays are not the only thing needed to demonstrate habitability. The bar is high: in brief, a mission needs to demonstrate the presence of water, key elements regarded as the building blocks of life (including carbon), and a source of energy. And you need to find them all together, and at the same instant in geologic time. In turn, each one of these must be characterized further to qualify an environment as having been habitable. Finally, it’s never black and white; understanding habitability is part of a broad continuum of environmental assessment, which is why orbiters and earlier rovers and landers are important assets in this process as well.

It is also important to define what group of organisms is being imagined to have inhabited the environments – their requirements will vary. Single-celled microorganisms are a great place to start based on our understanding of the early evolution of life on Earth, which was dominated by microbes for at least the first two billion years of the planet’s history. More specifically, the Curiosity team has been focusing on the conditions of habitability relevant to "chemolithotrophs," a group of microbes that feeds on chemical energy available in rocks.

Water. The water of a habitable environment should be relatively fresh, or at least not contain so much salt that the relative abundance of water is so low (what chemists call "water activity") that the osmotic pressure on cells would cause them to collapse. My favorite analog here is honey. Yes, it’s an aqueous environment but no, it’s not habitable: the sugar content is so high that microbes can’t live in it . This is why honey doesn’t spoil when not refrigerated. Salt serves the same role as sugar; too much salt inhibits life. Acidity is also important, although microbes have been shown to tolerate an extraordinary range of pH, including the very lowest values encountered in natural environments on Earth. However, more moderate pH favors a greater diversity of microorganisms, and thus more options to explore for emerging life forms. Finally, the water needs to last a long time on the surface; the longer, the better. A flow of water emerging on the surface of Mars from an underground source and boiling off in the presence of Mars’ modern low atmospheric pressure is not a good scenario for life. A stable source, such as a very ancient lake, with associated streams, and water flowing through the ground beneath it, is much better. We envision for the lake/stream/groundwater system that Curiosity discovered at Yellowknife Bay that the water could have existed for millions of years potentially. But even shorter periods are viable – the qualitative point here is that the rocks at Yellowknife Bay record more than a one-time event.

Key building blocks of life. A conventional list of key elements for life will include "CHNOPS" – carbon, hydrogen, nitrogen, oxygen, phosphorus, and sulfur. Previous orbiter and landed missions have provided ample evidence for H, O, and S via observations of sulfate and clay minerals, and P was measured by earlier rovers and landers. Curiosity has done the same. The tricky stuff is N and C and, along with P, they must all be “bioavailable”, which means to say they cannot be bound tightly within mineral structures that water and microbial chemical processes could not unlock. Ideally, we are looking for concentrated nitrogen- and phosphorous-bearing sedimentary rocks that would prove these elements were actually dissolved in the past water at some point, and therefore could have been available to enable microorganism metabolism. But in the interim Curiosity has been able to measure N as a volatile compound via pyrolysis (heating up rock powder in the SAM instrument), and P is observed in APXS data. We feel confident that N was available in the ancient environment, however we must infer that P was as well. Two of the Science papers, Grotzinger et al. and Ming et al., discuss this further. (You can download those and the other papers listed in this blog entry here.)

Carbon is the elephant in the room. We’ll discuss organic carbon further below, but here it’s important to make one very important point: organic carbon in rocks is not a hard-line requirement for habitability, since chemoautotrophs can make the organics they need to build cellular structures from metabolizing carbon dioxide (CO 2 ). These organisms take up inorganic carbon as CO 2 dissolved in water to build cellular structures. Organic carbon could serve as fuel if it was first oxidized to CO 2 , or could be used directly for biomass, or could be part of waste products. As applied to Mars it is therefore attractive to appeal directly to CO 2 , presumed to have been abundant in its early atmosphere. Curiosity does indeed see substantial carbon generated from the ancient lake deposits we drilled. The CO 2 that was measured is consistent with some small amount of mineral carbon present in those lake mudstones. These minerals would represent CO 2 in the ancient aqueous environment. Furthermore, it is possible that Martian organic sources have been mixed with inorganic sources of carbon in the mudstone; however, any organic contributions from the mudstone would be mixed with Earth-derived sources during analysis (see Ming et al. paper).

Energy. All organisms also require fuel to live and reproduce. Here it is essential to know which kind of microorganism we’re talking about, since there are myriad ways for them to harvest energy from the environment. Chemolithotrophs derive energy from chemical reactions, for example by oxidizing reduced chemical species like hydrogen sulfide or ferrous iron. That’s why Curiosity’s discovery of pyrite, pyrrhotite, and magnetite are so important (see Vaniman et al. and Ming et al. papers). They are all more chemically reduced than their counterparts discovered during earlier missions to Mars (for example, sulfate and hematite). Chemolithotrophic microbes, if they had been present on Mars at the time of this ancient environment, would have been able to tap the energy in these reduced chemicals (such as hydrogen sulfide, or reduced iron) to fuel their metabolism. If you are interested in more detail regarding these kinds of microbial processes I can strongly recommend Nealson and Conrad (2000) for a very readable summary of the subject.

The next section describes where I think we’re headed in the future. We’ll continue to explore for aqueous, habitable environments at Mt. Sharp, and along the way to Mt. Sharp. And if we discover any, they will serve as the starting point for seeing if any organic carbon is preserved and, if so, how it became preserved.