President Trump Donald John TrumpFederal prosecutor speaks out, says Barr 'has brought shame' on Justice Dept. Former Pence aide: White House staffers discussed Trump refusing to leave office Progressive group buys domain name of Trump's No. 1 Supreme Court pick MORE went after former Vice President Joe Biden Joe BidenFormer Pence aide: White House staffers discussed Trump refusing to leave office Progressive group buys domain name of Trump's No. 1 Supreme Court pick Bloomberg rolls out M ad buy to boost Biden in Florida MORE and lashed out at the Democratic presidential candidate's son Hunter Biden during his rally Thursday night.

Trump accused Biden of being a good vice president because he "understood how to kiss Barack Obama Barack Hussein ObamaDemocrats ramp up pressure on Lieberman to drop out of Georgia Senate race The Hill's Campaign Report: Biden on Trump: 'He'll leave' l GOP laywers brush off Trump's election remarks l Obama's endorsements Trump pledges to make Juneteenth a federal holiday, designate KKK a terrorist group in pitch to Black voters MORE's ass," sparking sustained cheers at the event center in Minneapolis, Minn.

Some of Trump's sharpest attack lines were directed at Biden during his sprawling two-hour remarks, with the president repeatedly asking where the younger Biden was.

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”Joe’s son Hunter got thrown out of the Navy and then he became a genius on Wall Street in about two days,” Trump said while targeting the Democratic presidential candidate's son.

“Whatever happened to Hunter? Where the hell is he?” he added. “Where’s Hunter?”

“Hey fellas, I have an idea for a new T-shirt,” the president continued, turning to the audience. "Let's do another T-shirt: 'Where's Hunter?'"

Earlier in the rally, the president's son Eric Trump Eric Frederick TrumpJudge orders Eric Trump to comply with New York AG's subpoena before Election Day Eric Trump uses misleading clip to blast Biden for using teleprompter Melania Trump: Ginsburg's 'spirit will live on in all she has inspired' MORE also went after Hunter Biden while overseeing a chant of "lock him up" when attacking the former vice president.

Biden, who had joined eight other candidates for an LGBT forum hosted by CNN and the Human Rights Campaign (HRC) on Thursday night, slammed Trump later in the evening on Twitter following the rally.

"I spent my night at the HRC forum talking about the fundamental respect every human being deserves. You spent yours showing how little respect for anyone else you have. America is so much stronger than your weakness, @realDonaldTrump," Biden tweeted.

I spent my night at the HRC forum talking about the fundamental respect every human being deserves. You spent yours showing how little respect for anyone else you have. America is so much stronger than your weakness, @realDonaldTrump. — Joe Biden (@JoeBiden) October 11, 2019

President Trump in recent days has pushed unsubstantiated allegations that the Bidens acted in a corrupt manner when dealing with Ukraine, focusing on Hunter Biden's role on the board of a Ukrainian energy company that had faced scrutiny in the country.

Trump has focused on Biden's efforts as vice president in 2016 to push for Ukraine to fire a prosecutor who had looked into the company, though no evidence has emerged that Biden acted with his son's interests in mind and both Bidens have denied any wrongdoing.

House Democrats have launched an impeachment inquiry into the president after revelations that Trump asked Ukrainian President Volodymyr Zelensky to look into Joe Biden during a July 25 phone call.

Trump has denied wrongdoing and has since publicly encouraged Ukraine and China to probe the former vice president.

Hunter Biden was discharged from the Navy Reserve in 2014 after reportedly testing positive for cocaine.

The Hill has reached out to the Biden campaign for comment on Trump's Thursday night remarks.

Updated: Oct. 11 at 12:40 a.m.