It does not take long to recognize that the B110 bus in Brooklyn is not like others in the city.

The exterior colors are different: red, white and blue. The price for a single ride is the same, $2.50, but MetroCards are not accepted. The bus does not run Friday night or most of Saturday.

But the most obvious sign that the B110 is different was demonstrated Wednesday by Gitty Green, a 30-year-old mother who boarded the bus on Wednesday with her three children and a stroller and headed straight to the back.

As her two older sons perched on the seats behind her, she looked ahead at the men seated in front, mostly Hasidic Jews in wide-brimmed hats, and said, because her religion dictates the separation of the sexes, she never wondered what it would be like to sit with them.

“It’s such a normal thing for us that women and men are separate,” she said. “Most of the ladies go to the back.”