The owner of an illegal puppy farm will be prosecuted after authorities seized more than a dozen dogs from "appalling" conditions on the outskirts of Melbourne.

Police and Wyndham Council raided one of the state's longest-running puppy farms on Tuesday after receiving a tip-off from animal rights activists.

The puppy farm in Little River raided by police and council officers.

The raid followed a complaint on Tuesday morning from Oscar's Law founder Debra Tranter, who said dogs were still being bred in horrific conditions at the property despite a council investigation into the puppy farm earlier this year.