An anti-vaccine campaigner accused of impersonating a government official at a Melbourne medical clinic could face criminal action.

Outspoken activist Jeff O'Toole confronted receptionists at a doctor's surgery in Coburg over vaccination posters they had put in the reception area.

In a seven-minute video posted online, Mr O'Toole can be heard telling staff they need to be removed.

Outspoken activist Jeff O'Toole confronted receptionists at a doctor's surgery in Coburg over vaccination posters they had put in the reception area. (9News)

During the exchange, Mr O'Toole handed over a copy of the TGA guidelines to the clearly confused staff member (9News)

"My name's Jeff, I'm here to inform you that this surgery is in breach of Therapeutic Goods Administration (TGA) Guidelines," he said in the clip.

"Advertising vaccine posters is a violation of their rules and regulations, so I'm required to basically come in here and inform you of that."

During the exchange, Mr O'Toole handed over a copy of the TGA guidelines to the clearly confused staff member.

He also asked to see a doctor before demanding the posters be removed.

"I require these posters to be taken down immediately, if not, further action will be taken," he can be heard saying.

Outspoken activist Jeff O'Toole confronted receptionists at a doctor's surgery in Coburg over vaccination posters they had put in the reception area. (9News)

But the TGA has defended the clinic, telling 9News it did not breach any guidelines.

"In terms of the regulation of advertising they were not breaking the law," Acting Chief Medical Officer Professor Paul Kelly told 9News.

"This is someone that appears at least to a casual observer to be purporting to be from the TGA and this is clearly not the case."

The TGA has asked YouTube to remove the video.

Mr O'Toole declined a request for interview but denied he impersonated any government official, saying he never explicitly said he was from the TGA.

TGA's Acting Chief Medical Officer Professor Paul Kelly told 9News the clinic didn't breach any guidelines (9News)