Margaret Sanger's birth control clinic was located at 46 Amboy St. in Brownsville, Brooklyn. View Full Caption Alexander Sanger

On the morning of Oct. 16, 1916, a long line of Brooklyn residents and their baby carriages formed outside a Brownsville brownstone.

"Can you afford to have a large family?" read the leaflets that summoned them in English, Italian and Yiddish.

"Do you want any more children? If not, why do you have them? Don’t kill, don’t take life, but prevent. Safe and harmless information can be obtained from trained nurses at 46 Amboy Street."

Almost 500 women from the Italian and Jewish immigrant families of the neighborhood visited the two-room clinic for advice on and demonstrations of contraceptive methods — before the police shut the operation down and arrested staff for violating a New York State law that banned birth control on the grounds of obscenity.

The backlash was just what clinic founder Margaret Sanger, a former obstetric nurse working on the Lower East Side, expected.

After touring the country with the message of contraception as a means of family planning for all Americans, the activist resettled in New York City with a plan to challenge state law and kindle public support for her cause.

Enlisting the help of a registered nurse, her sister Ethyl Byrne, and an interpreter, Fania Mindell, Sanger provided services that defiantly championed a woman's right to control her own fertility.

"I absolutely believe she was doing it as a statement to violate the law and get herself arrested," said the activist's grandson and former president of Planned Parenthood of New York City, Alexander Sanger.

"She knew to change the law she had to break it."

Living in the West Village, Margaret Sanger drew inspiration from the city's liberal intellectual circles of socialists, anarchists and labor unionists.

"In this early progressive era, there was a lot of optimism about how one could bring about change in the world," said her grandson, who still lives in Manhattan.

Sanger's clinic remains a "very radical statement," said Joan Malin, the current president and CEO of Planned Parenthood of New York City. "Here we are in 2016 and we're still fighting that [birth control] battle."

Tracing its roots back to the Brownsville clinic, 100 years on Planned Parenthood now serves 2.5 million patients in about 600 centers across the U.S.

"We're the only national network of [women's] health care services and it all started with Margaret Sanger's clinic in Brooklyn," Malin said.

Trace the origin story of the Brownsville clinic through our timeline below:

Timeline sources include the Margaret Sanger Papers Project, a Planned Parenthood report, and an interview with Alexander Sanger.