Division I (19 teams, entering in the Fourth Round) - Major League Soccer: Atlanta United FC, Chicago Fire, Colorado Rapids, Columbus Crew SC, D.C. United, FC Dallas, Houston Dynamo, LA Galaxy, Minnesota United FC, New England Revolution, New York City FC, New York Red Bulls, Orlando City SC, Philadelphia Union, Portland Timbers, Real Salt Lake, San Jose Earthquakes, Seattle Sounders FC, Sporting Kansas City

Division II (24 teams, entering in the Second Round) - North American Soccer League (6): Indy Eleven, Jacksonville Armada, Miami FC, New York Cosmos, North Carolina FC (formerly Carolina RailHawks), San Francisco Deltas; United Soccer League (18 teams): Charleston Battery, Charlotte Independence, Colorado Springs Switchbacks FC, FC Cincinnati, Harrisburg City Islanders, Louisville City FC, OKC Energy FC, Orange County SC (formerly Orange County Blues), Phoenix Rising FC (formerly Arizona United), Pittsburgh Riverhounds, Reno 1868 FC, Richmond Kickers, Rochester Rhinos, Sacramento Republic FC, Saint Louis FC, San Antonio FC, Tampa Bay Rowdies, Tulsa Roughnecks FC

Open Division Teams (56 total, all entering in the First Round):

Local Qualifiers (17 places, listed alphabetically by state): California (6): Chula Vista FC, El Farolito, La Máquina*, LA Wolves FC*, Moreno Valley Fútbol Club, Outbreak FC*; Colorado (2): Azteca FC, Colorado Rush; Florida (2): Boca Raton Football Club*, Red Force FC; Maryland (1): Christos FC; Massachusetts (1): GPS Omens; Nevada (1): Anahuac FC; New Jersey (1): FC Motown; Pennsylvania (2): Junior Lone Star FC, Tartan Devils Oak Avalon; Texas (1): NTX Rayados

Premier Development League (21 places): Division Winners: Charlotte Eagles* (N.C.), Des Moines Menace* (Iowa), FC Tucson* (Ariz.), Fresno Fuego (Calif.), GPS Portland Phoenix* (Maine), Michigan Bucks*, OKC Energy U23 (Okla.), Reading United AC* (Pa.), The Villages SC* (Fla.); At-Large Berths: Burlingame Dragons* (Calif.), Carolina Dynamo* (N.C.), Chicago FC United (formerly Chicago Fire U-23), Derby City Rovers (Ky.), FC Golden State Force (Calif.), Ocean City Nor’easters (N.J.), San Diego Zest (Calif.), SC United Bantams (S.C.), Sounders FC U-23* (Wash.), South Florida Surf, Ventura County Fusion* (Calif.), Western Mass Pioneers. The PDL is a nationwide league affiliated with the U.S. Adult Soccer Association and opted to use 2016 league results to determine its qualifiers for the 2017 Open Cup.

National Premier Soccer League (18 places): Qualified via 2016 NPSL playoffs: AFC Cleveland* (Ohio), Albion SC Pros (Calif.), Chattanooga FC* (Tenn.), Clarkstown SC Eagles* (N.Y.), Grand Rapids FC (Mich.), Miami United FC (Fla.), New Jersey Copa FC (N.J.), Sonoma County Sol (Calif.); At-Large Berths: AFC Ann Arbor (Mich.), Atlanta Silverbacks*, Boston City FC (Mass.), Dutch Lions FC (Texas), FC Wichita* (Kan.), Fredericksburg FC* (Va.), Jacksonville Armada U-23 (Fla.), Legacy 76 (Va.), OSA FC (Wash.) Tulsa Athletics (Okla). The NPSL is a nationwide league affiliated with the U.S. Adult Soccer Association and opted to use 2016 league results to determine its qualifiers for the 2017 Open Cup.