(a) Numbers of Lin−CD45+ small intestine lymphocytes from naïve R26DTA/+Ncr1-iCre and their respective Ncr1-iCre controls. Counts were obtained from flow cytometry plots showing intracellular RORγt and surface NKp46 expression. Numbers show cell counts within the indicated gate. Data were pooled from at least three independent experiments (n=2-4 mice per group in each) and are shown as the mean ± s.d. Statistical analyses were performed with unpaired Student’s t-test. **P < 0.01. (b) Characterization of colon ILCs in R26DTA/+Ncr1-iCre mice at steady state. Flow cytometry analyses (left panels) showing expression of RORγt and NKp46 within Lin−CD45.2+ cells from the colons of the indicated mice. Representative profiles show the frequency of the indicated populations (right panel). Data are pooled from two independent experiments (n=6 mice per group in total) and show the mean. Statistical analyses were carried out with unpaired Student’s t-test, ****P < 0.0001. (c-f) R26DTA/+Ncr1-iCre and Ncr1-iCre control mice were infected with 1010 CFU of C. rodentium. (c) The percentages of several myeloid and lymphoid populations were assessed in the colonic lamina propria, draining mesenteric lymph nodes and spleen from R26DTA/+Ncr1-iCre and Ncr1-iCre control mice, 12 days after infection. (d,e) Total cells were restimulated with PMA/ionomycin for 4 hours in the presence of brefeldin, to assess the percentage of IFN-γ- and IL-17A-producing CD4+ and CD8+ T cells, as indicated. Data shown are representative of two independent experiments. (f) Explants of distal colon were weighed, homogenized and incubated for 24 hours in complete RPMI. Cytokine levels were determined in the supernatants. Data shown are one of two independent experiments (n=6-7 mice per group in each) and show the mean.