Controversial AFL Footy Show host Sam Newman has again been slammed for making homophobic comments after he accused American footballer Michael Sam of an "annoyingly gratuitous act" by kissing his boyfriend on TV.

While it was a gay activist who fought Newman's anti-gay comments a decade ago, this time it was co-hosts Garry Lyon and James Brayshaw who told the 68-year-old that he was "homophobic" and "off the pace".

Several in the audience of Wednesday night's show also booed Newman, who insisted that "no heterosexuals do that when they're drafted".

Lyon disagreed. "You always see shots of players drafted in basketball or footy, whether they're with their girlfriends, wives, partners — of course they embrace and kiss," he said.