Fruit and vegetables are running out at some Coles stores across Victoria, after hundreds of warehouse workers walked off the job and shut down the supermarket giant's primary cold storage facility.

On the third day of industrial strife at Polar Fresh, which stores all chilled food for Coles statewide, the effect of union members' strike action and blockades became apparent as shoppers found fresh produce shelves empty.

Workers picket a cold storage warehouse that supplies Coles.

"Unfortunately, some of our deliveries to Victorian stores have been disrupted due to union industrial action at Polar Fresh, which provides warehousing facilities for Coles in Victoria," a Coles spokesman said on Friday.

"Deliveries are now flowing through to stores and we are working hard to ensure all products are on shelf as soon as possible. We apologise to our customers for any inconvenience."