GREENVILLE, S.C. — Tensions among three of the Republican presidential hopefuls vying for support from the establishment wing of the party burst into public view this week, suggesting a new, more combative phase of a campaign that has been dominated by attacks between Donald J. Trump and nearly all of his rivals.

The crossfire began on Wednesday in New Hampshire when Jeb Bush compared Senator Marco Rubio’s message of generational change to that of President Obama’s. And it escalated on Thursday in Iowa, where Mr. Rubio’s staff ejected a videographer working for a “super PAC” supporting Mr. Bush from a campaign event — and then took a more personal turn on Friday when Gov. John R. Kasich of Ohio mocked Mr. Bush’s decline in the polls.

With Mr. Trump still at the top of the polls as the calendar moves to October, the other candidates feel a growing urgency to stand out as more voters begin paying attention to the Republican contest and the bombastic casino magnate continues to command much of the media coverage of the race. Mr. Bush, especially, is campaigning more aggressively as he slips in many national and state polls.

Introducing himself at a policy forum on the campus of Furman University here Friday, Mr. Bush said that in his time as the governor of Florida he “disrupted the old order” and “turned the place upside down.”