The molecular components of the ligand-inducible gene circuit were kindly provided by the Doktycz lab. The plasmid pSB3C5-proD-B0032-E0051 was a gift from J. Davis and R. Sauer (Addgene plasmid no. 107241). J.B.C. was funded by an Ontario Graduate Scholarship. This work was supported by the NSERC Discovery Grants Program (RGPIN-2016-06352), the CIHR Foundation Grant Program (201610FDN-375469), The University of Toronto’s Connaught New Research Award and the CIHR Canada Research Chair Program (950-231075) to K.P.; the University of Toronto’s Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund (C1TPA-2016-06), and the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R21AI136571 (the content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health) to K.P. and S.O.K.; an NIH Director’s New Innovator Award (1DP2GM126892), an Arizona Biomedical Research Commission New Investigator Award (ADHS16-162400), an Alfred P. Sloan Research Fellowship (FG-2017-9108), Gates Foundation funds (OPP1160667), and Gordon and Betty Moore Foundation funds (no. 6984) to A.A.G. We thank S. Cicek for her help enhancing the high-throughput data analysis. We thank M. Labib and C. Nemr for their advice and support of the project.