The 16-year-old performer who sang the national anthem at Donald Trump's inauguration has spoken out against the president's decision to repeal an Obama directive on transgender bathrooms.

Trump's administration announced on Wednesday evening it has lifted federal guidelines that said transgender students should be allowed to use public school bathrooms and locker rooms matching their chosen gender identity.

It is a reversal of an Obama-era directive issued in May. It will now be up to states and school districts to interpret whether federal sex discrimination law applies to gender identity.

Jackie Evancho, the 16-year-old who performed at Trump's inauguration (pictured), has spoken out against the president's decision to repeal an Obama directive on transgender bathrooms

Just hours before the announcement was made, Jackie Evancho pleaded with the president to have a discussion about transgender rights.

'I am obviously disappointed in the @POTUS decision to send the #transgender bathroom issue to the states to decide. #sisterlove,' she wrote.

'@realDonaldTrump u gave me the honor to sing at your inauguration. Pls give me & my sis the honor 2 meet with u to talk transgender rights.'

Evancho's older sister, Juliet, is transgender.

Evancho sent these two tweets on Wednesday just hours before Trump's decision was announced

'I am obviously disappointed in the @POTUS decision to send the #transgender bathroom issue to the states to decide. #sisterlove,' Evancho (pictured at the inauguration) wrote

But it was for nothing, as Trump's team formalized the decision on Wednesday by sending a letter to schools nationwide by the Justice and Education departments.

The letter claimed the previous protections caused confusion and lawsuits over how it should be applied.

Although the Obama guidance was not legally binding, transgender rights advocates say it was necessary to protect students from discrimination.

Opponents argued it was federal overreach.

Jackie (left) and Juliet (right) Evancho are seen together in October 2015 in New York City

Pictured is the letter that was sent to schools across the country announcing the decision on Wednesday

Donald Trump discusses the federal budget in the Roosevelt Room of the White House on February 22, 2017 in Washington

Backlash against Trump's decision came quickly on Wednesday night, with many blasting the reversal on social media.

'Trump's cowardice is markedly on show this evening. Transgender kids are braver than he and the rest of us put together,' Stephen Brown wrote.

'The Trump administration rolling back protections for transgender students is inhumane. Trans communities deserve our respect,' youth group, United We Dream, said.

'Trump's action sends a terrible message to transgender students. But these students should know the law is on their side,' the ACLU tweeted.

'If there is a single consistent thing that defines Trump, it's his penchant for cruelty. Why make hurting transgender kids a priority?,' Lily Mazahery wrote.

'80% of transgender K-12 students are bullied. Trump's cruel removal of protections for trans kids puts the government on the side of bullies,' Duncan Hoosie tweeted.

'That didn't take long. Such a sneaky way to go back on his promise to the LGTBQ community,' another person said.

'Sad -- why? These kids are not hurting anyone - pathetic pandering to base!' another tweeted.

'Ok, who exactly does it hurt to protect transgender students? Oh, that's right - no one,' Sue Larson said.

'Man, repealing those laws protecting transgender kids is gonna create SO many jobs,' Russ Bengston added.