A prostitute linked to the overdose deaths of at least three men, including Cipriani Dolci head chef Andrea Zamperoni, pleaded not guilty Friday to causing the July 11 demise of a man who succumbed to a fentanyl overdose.

According to an indictment unsealed in Brooklyn federal court, prosecutors say Angelina Barini, 41, sold a fatal hit of fentanyl to a man identified in court papers as John Doe No. 1 in a motel room in Woodside, Queens.

So far, Barini has been formally charged only with the anonymous man’s overdose death, but prosecutors allege in court papers that she is linked to the overdose deaths of two more men.

While the John Doe victim has not been identified in court papers, the Office of the Chief Medical Examiner reported that Jean-Alessandro Silvero died on July 11 from fentanyl intoxication at a Woodside motel. Silvero’s mother was in attendance at Barini’s initial court appearance in August.

“In her course of business as a prostitute, it is alleged that Barini gave her client a fatal dose of fentanyl resulting in his death,” Peter Fitzhugh, special agent-in-charge of the New York office for Homeland Security Investigations, said in a news release.

Mildred Whalen, a public defender who appeared with Barini at the arraignment, said her office plans on “mounting a vigorous defense to the charges.”

Zamperoni was found dead on Aug. 21 inside the Kamway Lodge & Tavern in Elmhurst. The medical examiner’s office said on Friday that Zamperoni’s death was accidental and that GHB, alcohol and cocaine were found in his system.

According to court papers, authorities arrested Barini after she answered the door at room 15 at the motel and found Zamperoni’s dead body inside stuffed in a garbage can.

Following her arrest, Barini was charged at the time with a single count of fentanyl distribution, and has been locked up ever since.

NYPD Chief of Detectives Dermot Shea said on Sept. 6 that police are investigating a fourth overdose death that may be tied to Barini, and police sources told The Post they might be looking into a fifth victim.