Lab Carbon Dioxide Removal Assembly (CDRA) Maintenance: Yesterday the crew installed the newer generation beds, like those in Node 3 CDRA, into Lab CDRA. Today, the crew repaired the CDRA Valve Power Cable W9P12 by splicing in two new wire segments and performing a 120V power check of the connector prior to installing CDRA into the LAB AR Rack. Initial CDRA power and data checkout was nominal. The full Lab CDRA checkout is in work.

Pressurized Mating Adaptor (PMA) 3 Ingress Prep: The crew is scheduled to ingress the PMA3 tomorrow morning. Today the crew reviewed the PMA3 ingress procedure and gathered required hardware for tomorrow’s activities.

Energy Requirements for Long-Term Space Flight (Energy): Today is Day 1 for the Energy investigation; yesterday the subject began the 11 day diet and today began the urine collection for both the subject and control subject. This morning, the subject configured the Pulmonary Function System (PFS) and performed a series of Oxygen Uptake Measurements (OUMs) after prescribed meals and scheduled fluid collections. The Energy investigation measures adaptations in the components of total energy expenditure of crewmembers, so that an equation to determine the energy requirements of crewmembers during space flight may be derived. Such knowledge of energy requirements is of a great importance to ensure health, good performance and the overall success of a mission, and also contributes to ensure adequate exercise load and cargo allotments for food during space flight.

Advanced Plant Experiments (APEX)-04 Photo: The crew retrieved one of the APEX-04 petri plates and photographed it on the Advanced Biology Research Facility (ABRS) photogrid. The picture will be downlinked for ground teams to evaluate the growth status. The APEX-04 (Epigenetic change in Arabidopsis thaliana in response to spaceflight – differential cytosine DNA methylation of plants on the ISS) experiment investigates how spaceflight can affect an organism on a molecular level without altering its outward appearance. Previous research has demonstrated that large numbers of plant genes are expressed differently in space compared to plants on Earth, which can alter how plants grow. This investigation studies the entire genome of thale cress plants grown in space, creating maps of spaceflight-specific changes in certain groups of genes. Results give new insight into plants’ molecular responses to spaceflight, which benefits efforts to grow plants in space for food and oxygen.

Microgravity Expanded Stem Cells (MESC) Microscope Operations: The crew removed a BioCell from the Space Automated Bioproduct Laboratory-2 (SABL2) and placed it on the microscope stage configured outside the Microgravity Science Glovebox (MSG). The crew worked with ground specialist to image areas of the BioCell plate. Following the focused imaging, the crew reinserted the BioCell back into the SABL. MESC will aid researches determine the efficiency of using a microgravity environment to accelerate expansion (replication) of stem cells for use in terrestrial clinical trials for treatment of disease.

ISS Reboost: Last night the ISS performed a nominal reboost using the SM main engines, TIG 061/03:10 GMT burn duration 43 seconds. The reboost set up the planned conditions for the upcoming landing of 48S on 10-April and launch of 50S on 20-April 20. The 50S launch will utilize the 4-orbit rendezvous.

Mobile Servicing System (MSS) Operations: Robotics operations for Stratospheric Aerosol and Gas Experiment (SAGE) Instrument Panel (IP) extraction from the SpX-10 Dragon Trunk have begun.

Today’s Planned Activities

All activities were completed unless otherwise noted.

Energy PFS Powerup

Energy Urine Sample Collection

Regeneration of БМП Ф1 Micropurification Cartridge (start)

Energy PFS Instrument Check

ENERGY Double Labeled Water Intake

In Flight Maintenance Carbon Dioxide Removal Assembly (CDRA) Preparation.

Energy Oxygen Uptake – Part 1

Pressurized Mating Adapter 3 Big Picture Word Review

IDENTIFICATION. Copy ИМУ-Ц micro-accelerometer data to laptop

Electrostatic Levitation Furnace(ELF) sample holder packing for SpX-10 early destow

Water Recovery System Waste Water Tank Drain Termination

In Flight Maintenance Carbon Dioxide Removal Assembly (CDRA) Preparation.

Standardized Breakfast

Replacement of БД-2 Treadmill Belt.

Energy Oxygen Uptake – Part 2

In-flight Maintenance (IFM) Carbon Dioxide Removal Assembly (CDRA) Chassis Installation

Empty bladder for ENERGY

Energy Oxygen Uptake – Part 3

Start transfer from CWC-I No.2379 (NODE1; 20.4 L) to EDV No.1234 (00068255R, ФГБ1ПГО1_109)

СОЖ maintenance

Terminate water transfer from CWC-I to ЕДВ

Progress 435 (DC1) Cargo Transfers and IMS Ops

Energy Urine Sample Collection

Energy Oxygen Uptake – Part 4

BIOPLENKA. Removal of Samples from the Thermostat, fixation of Constanta cassette No.1-6 biofilm.

Energy Urine Sample Collection

Lab Carbon Dioxide Removal Assembly (CDRA) Bed Equipment Stow

Energy Urine Sample Collection

BIOPLENKA. Removal of Cryogem-03 after Completion of the Experiment

APEX-04 Petri Plate Photo

Meteor Shutter Open

Rodent Research Access Unit Clean

Energy PFS Conclude

Progress 435 (DC1) Cargo Transfers and IMS Ops

Pressurized Mating Adapter 3 (PMA 3) Hardware Gather

ENERGY Diet Log of Lunch

Columbus Video Camera Assembly 1 Adjustment

Preparing For Upcoming MagVector Science Run

ESA Weekly crew conference

RS remote laptop testing after BRI cleaning.

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

Inventory Management System (IMS) Conference

Public Affairs Office (PAO) High Definition (HD) Config LAB Setup

Microscope Hardware Unstow

PAO Preparation

Delta file prep

Public Affairs Office (PAO) Event in High Definition (HD) – Lab

Dose Tracker Data Entry Subject

Micrgravity Expanded Stem Cells Microscope Ops

Activation/Deactivation of MPEG2 Multicast TV Data Monitoring a (Ku-band)

Cleaning dust filter cartridges ПФ1-4 in SM

Fluid Shifts Dilution Measures Setup

Download of BRI log from RSS1

CONTENT. Experiment Ops

JEM System Laptop Terminal Reboot

Flight Director/ISS CREW CONFERENCE

Microscope Hardware Temporary Stowage

Completed Task List Items

None

Ground Activities

All activities were completed unless otherwise noted.

Lab AR Rack Activation

Lab CDRA Checkout

SAGE IP Extract

Three-Day Look Ahead:

Friday, 03/03: Node 2 Power Cable (HMU 220) Rerouting, PMA3 Ingress, ESA Energy, Node 3 IMV Valve Scavenge

Saturday, 03/04: Weekly Housekeeping, Microgravity Expanded Stem Cells (MESC) Session

Sunday, 03/05: Microgravity Expanded Stem Cells (MESC) Session

QUICK ISS Status – Environmental Control Group: