The prosecution case against a Victorian police officer charged with the murder of a motorist in Windsor two-and-a-half years ago will rely on audio and video recordings from the accused officer's car.

Victoria Police senior constable Timothy Baker appeared before the Melbourne Magistrates' Court charged with one count of shooting Vlado Micetic dead.

The 45-year-old is alleged to have pulled Micetic over late on the evening of August 25, 2013, on Union Street in Windsor.

The court heard the prosecution intends to play audio and video of the incident recorded in Baker's police car.

The prosecution is expected to call more than 20 witnesses - including police officers, DNA experts, the passenger who was in the car at the time of the shooting and members of Mr Micetic's family.

Mental health reports are expected to be used for both Baker and Mr Micetic.

Baker has been suspended from Victoria Police while the case is ongoing.

He was charged in October and is currently on bail.

The committal hearing is expected to begin on August 1 and has been set down for seven days.