Marrow: Upon wakeup a 53S crewmember collected breath and ambient air samples. With operator assistance, the subject collected blood samples to support the Marrow investigation. The blood samples were processed in the centrifuge and placed in the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). The Marrow investigation looks at the effect of microgravity on bone marrow. It is believed that microgravity, like long-duration bed rest on Earth, has a negative effect on the bone marrow and the blood cells that are produced in the bone marrow.

Synthetic Bone BioCell Operations: During yesterday’s scheduled microscope operations for BioCell Habitats A and B, microscope issues inside the Microgravity Science Glovebox (MSG) prevented ground teams from viewing video footage of the growing bone cells from the BioCells. With the deferment of the fixation of BioCell Habitat A, the crew was able to complete one of today’s media exchanges during yesterday’s operations. Ground teams worked to resolve the issue with the microscopy video downlink and rescheduled today’s activities. This morning, using the MSG laptop screen as a workaround for the non-functional video monitor, the crew successfully completed the first microscopy of four Synthetic Bone Biocells, two each from Habitats A and B. Additionally, fixation of both Biocells from Habitat A was completed. The two Biocells from Habitat B were re-inserted into SABL for continued incubation and growth. The crew later continued Synthetic Bone operations by performing a media change for Habitat B. Synthetic Bone uses BioCell habitats A, B, and C. The BioCell habitats will be used to test the functionality and effectiveness of new material that can assist in recovery from bone injuries or dental work during long-term space travel. Determining how well Tetranite integrates with bone cell cultures can also inform general strategies for addressing bone loss in space. Synthetic Bone examines the cellular response to a new type of bone adhesive in the microgravity environment of space. This experiment uses facilities aboard the International Space Station (ISS) to grow bone cells in the presence of a commercially available bone adhesive, and a new product called Tetranite. Sets of bone cell cultures grow with the different adhesives for 20 days and are then fixed, frozen, and returned to Earth for detailed analysis in a fully equipped biological laboratory.

MagVector: Today the crew performed setup activities and began the 7-day MagVector #15 experiment run. The European Space Agency (ESA) MagVector investigation studies how Earth’s magnetic field interacts with an electrical conductor. Using extremely sensitive magnetic sensors placed around and above a conductor, researchers can gain insight into ways that the magnetic field influences how conductors work. This research not only helps improve future International Space Station experiments and electrical experiments, but it could offer insights into how magnetic fields influence electrical conductors in general, the backbone of our technology.

Zebrafish Muscle 2: After completing yesterday’s fixation operations for the experiment units (EUs) from Groups B and C, today the crew transferred the EUs from the refrigerator to the freezer of the MELFI. The EUs will remain in the MELFI freezer until they are returned on SpaceX-13. The results from Zebrafish Muscle 2 operations will help to determine whether atrophy of muscles under microgravity also occurs in zebrafish, and why that muscle atrophy occurs in microgravity. Physical exercise and control of posture are important for maintaining muscle mass and strength. In microgravity conditions, the postural, known as anti-gravity muscles, undergo atrophy because of prominent decrease in their gravity-dependent activity.

APEX-05 Operations: Following yesterday’s installation of twenty APEX-05 petri plates into the VEGGIE facility to initiate the growth process, today the crew photographed the four spare petri plates that are secured to the Advanced Biology Research Facility (ABRS) photo grid on the maintenance work area. When plants are grown in the confines of the International Space Station (ISS), they do not seem to get enough air and as a result, exhibit a stress response in their genes and proteins. The Spaceflight-induced Hypoxic/ROS Signaling (APEX-05) experiment grows different wild and mutant varieties of Arabidopsis thaliana in order to understand how their genetic and molecular stress response systems work in space. The plants grow from seeds in the Veggie plant growth facility aboard the ISS, are frozen, and returned to Earth for detailed laboratory analysis.

NanoRacks External Platform (NREP) transfer to JEM Airlock (JEM A/L): Overnight, JAXA ground teams removed NREP from JEM External Facility (JEM EF) Exposed Facility Unit (EFU) site 4 to the JEM A/L. In early January, the crew will remove NREP from the JEM A/L, exchange samples on the platform, and then work with ground controllers to return NREP to EFU #4.

SSRMS Latching End Effector (LEE) Survey: After some anomalous data was observed during last weekend’s Dragon capture, Robotics ground controllers and the crew worked together to inspect the LEE for any potential damage induced. Later in the day, ground controllers maneuvered the SSRMS to grapple the fixture on the PMM to gather loads data for further analysis. Results are pending.

Today’s Planned Activities

All activities are complete unless otherwise noted.



Marrow Breath And Ambient Air Sample Collection – Subject

CSA Generic Frozen Blood Collection 25 Minutes – Subject

CSA Generic Frozen Blood Collection – Operator

CSA Generic Refrigerated Centrifuge Configure

SSRMS LEE Survey

CSA Generic Refrigerated Centrifuge Spin Conclude

On-Board Training (OBT) Dragon Emergency Review

CSA Generic Sample MELFI Insertion

Preparation and setup of КСПЭ Equipment for MPEG2 video recording via Ku-band.

Health Maintenance System (HMS) ISS Food Intake Tracker (ISS FIT)

ISS Crew Adaptation

Synthetic Bone Microscope Operations

Replacement of БД-2 Treadmill belt. Tagup with specialists

ISS HAM Service Module Pass

ISS Crew Orientation

Columbus SUP Ground Fault Interrupt Test preparation

Columbus SUP Ground Fault Interrupt Test

Synthetic Bone Biocells Fixation

Preparing For Upcoming MagVector Science Run

Personal CO2 Monitor iPad Data Collection

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

Columbus GFI Test – Inverter reconfiguration

In Flight Maintenance (IFM) Waste and Hygiene Compartment (WHC) Full Fill

Columbus SUP Ground Fault Interrupt Test closeout.

CSA Generic Frozen Blood Collection Conclude And Stow

MELFI Sample Return Bag Insertion

MELFI BioCell Habitat Insertion

CEVIS Handover Video Review

MELFI Fixative Return Bag Insertion

CEVIS Crew Handover

Made In Space Fiber Optics Locker and Cable Setup

КСПЭ Hardware Deactivation after video recording

Glacier Media Kit Remove

Emergency Roles and Responsibilities Review

CFE Generic Frozen Blood Collection Setup

Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Drain

Synthetic Bone Biocells Media Ops

APEX-05 MWA Preparation

On MCC GO Filling up Progress 436 [AO] СрПК Section 2 with N2

Zebrafish Muscle 2 Temp Logger Data Save 2

On-Orbit Hearing Assessment (O-OHA) with EarQ Software Setup and Test

APEX-05 Spare Petri Plate Photo Day 1

Crew Medical Officer (CMO) Proficiency Training

Area Dosimeter Installation

Inventory Management System (IMS) Conference

COSMOCARD. Setup. Starting 24-hr ECG Recording

Rood Growth Camera Setup

Root Growth Experiment Activation

Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Remove and Replace

Health Maintenance System (HMS) Automated External Defibrillator (AED) Inspection

Polar 1 Crew Preference Insert

Transfer 2 Fish Fixation Apparatus from MELFI (+2C) to MELFI (-95C).

Space Headaches – Daily Questionnaire

Early Mission Debrief Conference

MELFI Sample Bag Insertion

Zebrafish Muscle 2 Temp Logger Data Setting

CB/ISS CREW CONFERENCE

iPad Air 2 Charge

Flight Director/ISS CREW CONFERENCE

Completed Task List Activities

Glacier Media Kit Remove

JAXA photo taking and report writing

MELFI Sample Bag Insertion

Synthetic Bone Biocells Media Ops

Ground Activities

All activities are complete unless otherwise noted.



Standard commanding

SSRMS support for LEE inspection

Three-Day Look Ahead:

Friday, 12/22: IMV Flow Measurement, FIR maintenance, Emer H/W OBT, CBM N2N Bolt Inspect, Cell Science Validation, JPM gas trap reconfig, JAXA Camera Robot, Blood Collection, APEX-5, STP-H5 ICE Photos, Space Headaches

Saturday, 12/23: Off duty

Sunday, 12/24: Off duty, APEX-5

QUICK ISS Status – Environmental Control Group: