Appalled at the images he has seen of abused and helpless puppies, Victorian Premier Ted Baillieu has vowed to put the bite on illegal puppy farms.

Speaking at the Lost Dogs' Home in North Melbourne on Sunday, Mr Baillieu outlined tough new penalties, massive fines for cruelty and equipping the RSPCA with new policing and confiscation powers to seize profits and assets from illegal puppy farms.

Legislation for the powers will be introduced in the Victorian parliament this week.

"We are not going to tolerate cruelty to animals," Mr Baillieu said.

"These are some of the strongest laws ever introduced to protect animals from abuse and neglect.