“Emotional Creature,” a new play by Eve Ensler (“The Vagina Monologues”) about the inner lives of girls across the world, will have its Off Broadway premiere this fall at Signature Center, the show’s producers said on Wednesday.

An ensemble of six young actresses, including Joaquina Kalukango (who played the teenager Cookie in Signature’s “Hurt Village” last winter), will perform monologues, stories, and songs that render the pressures and rites of passages of growing up female in disparate cultures. The director will be Jo Bonney (“By the Way, Meet Vera Stark”), who mounted the play for its ongoing world premiere at Berkeley Repertory Theater.

The New York production is scheduled to begin preview performances on Oct. 26 at Signature’s Romulus Linney Courtyard Theater, and open on Nov. 12.

The show, which is expected to run at least through mid-January, is not part of Signature’s subscription season; rather, the producers are renting the Linney, an income stream that is crucial to the nonprofit Signature as it transitions from its former single-theater home to the three stages in the new Signature Center.

Ms. Ensler, whose last play to run Off Broadway was “The Treatment” in 2006, adapted the new play from her 2010 book, “I Am An Emotional Creature: The Secret Life of Girls Around The World.”