a, Statistical analysis of the average liver sizes in wild-type, capn3a−/−, wild-type embryos injected with ST-MO, capn3a-MO or wdr5-MO, and capn3a−/− embryos injected with ST-MO or wdr5-MO at 3.5 dpf. b, qRT–PCR analysis of capn8 and capn12 mRNA expression in wild-type and capn3a−/− embryos injected with ST-MO or wdr5-MO as shown. The injection of ST-MO was used as a negative control and given a value of one. c, WISH of capn8 in the wild-type and capn3a−/− embryos at around 1 dpf. Left, wild-type and capn3a−/− embryos without injection. Right, wild-type and capn3a−/− embryos with wdr5-MO injection. Red arrow denotes the region with upregulated capn8 expression; green arrow denotes the region with downregulated capn8 expression after wdr5-MO injection. d, Relative mRNA expression of capn8 in wild-type embryos injected with or without wdr5-MO, the wdr5-overexpression plasmid (wdr5-p), or wdr5-MO together with wdr5 plasmid, and in capn3a−/− embryos injected with or without wdr5-MO, the wdr5-overexpression plasmid (wdr5-p), or wdr5-MO together with wdr5 plasmid. e, Schematic diagram showing the genomic structure and a genetic mutation of the zebrafish wdr5 gene. ATG denotes the translation start codon; black lines denote introns; vertical bars denote exons (blue for UTR regions, black for the coding region). The red underlined sequence denotes the additional DNA (10 bp) in the wdr5 mutant; the numbers in the fifth and sixth panels denote the amino acid positions of one domain in zebrafish Wdr5 protein, and the length of predicted Wdr5 mutant protein (Wdr5i10). f, WISH was performed to assess the liver sizes in 3.5-dpf-old wild-type and capn3a−/− embryos injected with different reagents as indicated. Ctr, uninjected control. g, Statistical analysis of the average liver sizes in f under the different treatments as indicated. h, Relative mRNA expression of capn8 and capn12 in 1.5-dpf-old wild-type and capn3a−/− embryos with different treatments as indicated. The injection of ST-MO was used as a negative control and given a value of one. i–k, qRT–PCR analysis of capn8 and capn12 mRNA expression in wild-type and capn3a−/− embryos injected with ST-MO or setd1a-2#MO (i), rbbp5-2#MO (j), or ash2l-2#MO (k). The injection of ST-MO was used as a negative control and given a value of one. Each experiment was repeated three times with similar results, and a representative result is shown. n indicates the number of zebrafish embryos in each group. Data are mean ± s.d. from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed t-test.