WASHINGTON — Vice President Mike Pence responded aggressively on Monday to the decision by President Vladimir V. Putin of Russia to order the shedding of 755 positions at the United States Embassy in Moscow, even as President Trump remained silent on the escalating diplomatic row.

Traveling in Eastern Europe, Mr. Pence delivered a blunt message for Mr. Putin, noting that “recent diplomatic actions taken by Moscow will not deter the commitment of the United States to our security, that of our allies and to freedom-loving nations around the world.”

Mr. Pence said that while Mr. Trump remains “open to a better relationship” with Russia, he warned that “the United States rejects any attempt to use force, threats, intimidation or malign influence in the Baltic States or against any of our treaty allies.”

Mr. Putin over the weekend ordered that the embassy work force be trimmed by Sept. 1, responding to legislation approved last week by Congress to impose new sanctions on Russia for its meddling in the 2016 presidential election. The legislation also imposes additional sanctions on Iran and North Korea.