Victoria Police says it will look into claims that a senior detective was pressured to drop a 2004 rape case against St Kilda footballer Stephen Milne.

Former detective Scott Gladman has told Channel Nine he was intimidated and threatened by other officers to "do the right thing".

He says he was told he would be looked after if the case was not pursued.

Milne was accused of raping a woman at the home of his team-mate, Leigh Montagna, in March 2004.

Both men were interviewed by police but were not charged and have always denied the allegations.

After a two-month investigation, Victoria's Director of Public Prosecutions declined to prosecute Milne, concluding there was insufficient evidence for charges to be laid.

Mr Gladman says Milne is innocent until proven guilty in a court of law, but claims the case never got that far because the natural flow of justice was interrupted.

Mr Gladman says one senior police officer, who was a passionate St Kilda fan, called him to issue a warning.

"[He said] 'It didn't happen the way she said it happened. She's just one of these footy s***s that runs around looking for footballers to f***', was the words," he said.

"You better make this go away. You better do the right thing. You better make sure that this is done properly."

Mr Gladman claims the police officer called him twice more.

"I was left [with] no doubt in my mind that people have long memories in the police force and that if I want to go far, or want to get on, then I have to do the right thing here," he said.

Mr Gladman says parts of his brief of evidence went missing, sometimes for several hours, including the statement by the woman who made the rape allegation.

"So the football club would have been aware of the allegations in a statement form, the victim's names and the witness's names, within days of it happening," he said.

"I was riding home at night time with four folders in my backpack, an extra 10-15 kilos, because I couldn't leave the briefs at work."

Victoria Police says the allegations have not been raised with the Police Ethical Standards department but that Victoria Police will now take a look at what has been said and decide what further actions might need to be taken.

Acting Deputy Commissioner Steve Fontana says the allegations are extremely serious and will be thoroughly investigated.

"We certainly would like to speak to Scott Gladman in relation to the allegations and also pulling out the file, the brief of evidence," he said.

"We need to speak to all the parties involved, we need to speak to the victim in this matter just to keep her informed about the progress of the investigation."

There is no suggestion that Milne or Montagna were involved in the alleged interference with the police investigation.