The furore over toilet paper panic-buying has escalated with two women charged in Sydney following an alleged assault at a supermarket that was captured on video.

The women, aged 23 and 60, have been charged with affray over the alleged fight at a Chullora Woolworths store on Saturday morning, sparked by an apparent dispute over toilet paper.

Footage of the incident quickly circulated on social media on Saturday, showing staff intervening in a dispute between three women. The staff member can be heard urging those involved to "back off right now" from a trolley stacked with jumbo packs of toilet rolls.

"I just want one pack," one woman says to another at the helm of the overflowing trolley.