One of Melbourne's most recognisable and enduring late-night venues has warned patrons caught using the party drug GHB will be banned for life.

Revolver Upstairs, which has built a reputation for its night-into-day weekend sessions, posted to its Facebook page on Wednesday it was "extremely concerned about a noted increase" in the use of the potentially deadly drug.

The Revolver nightclub. Credit:Joe Armao

"GHB can cause severe respiratory issues and may result in death and is particularly risky when consumed with alcohol," the post read.

"Revolver Upstairs is committed to safety and harm reduction and for this reason maintains a strict policy of issuing lifetime bans for anyone found to use GHB.