Kellyanne Conway has accused CNN anchor Anderson Cooper of sexism after he rolled his eyes during an interview with her this week.

The senior adviser to Donald Trump was defending the president's firing of FBI Director James Comey on Tuesday when the veteran broadcaster produced an exaggerated eye-roll that quickly went viral.

Drawing parallels between herself and Democratic presidential nominee Hillary Clinton, Ms Conway told “Fox and Friends”: “Let me tell you something: Hillary Clinton is in search of sexism as a lame excuse for why her disastrous candidacy and campaign lost six months ago.

"I face sexism a lot of times when I show up for interviews like that. Could you imagine rolling your eyes, having a male anchor on a network roll eyes at Hillary Clinton ... a female representative/spokesperson for President Obama or President Bill Clinton?”