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Embattled Manhattan District Attorney Cyrus Vance​ Jr.​’s top aide won’t take part in the rape prosecution of Harvey Weinstein because her husband works for the disgraced movie mogul’s new defense lawyer, a spokeswoman said Thursday.

Chief ADA Karen Friedman Agnifilo — who last month publicly defended Vance’s decision not to prosecute Weinstein in an alleged 2015 groping incident — recused herself from anything involving the recent flood of accusations against the perverted producer, Vance spokeswoman Joan Vollero said.

On Wednesday, Weinstein added high-p​rofile Manhattan lawyer Ben Brafman to his legal team, following last week’s revelation by the NYPD that “Boardwalk Empire” actress Paz de la Huerta made “credible and detailed” claims that Weinstein raped her seven years ago.

Vance could seek an indictment of Weinstein as early as next week, law enforcement sources have told The Post.

Friedman Agnifilo’s hubby, Marc Agnifilo, is senior trial counsel at Brafman’s law firm, and she has had to recuse herself from dozens of criminal cases due to their marriage, most notably Vance’s botched prosecution of French pol and Brafman client Dominique Strauss-Kahn in 2011.

Strauss-Kahn, then the head of the International Monetary Fund, was charged with sexually assaulting a Midtown hotel maid, but the case was dropped when prosecutors said the accuser had repeatedly lied about her background and “the circumstances of the incident.”

Vance came under fire last month following the New Yorker magazine’s release of a secretly made NYPD recording in which Weinstein apologized to model Ambra Battilana Gutierriez for grabbing her breast during a March 2015 meeting in his Tribeca office.

Vance had declined to prosecute that case, and Friedman Agnifilo issued a statement that blamed cops for making the recording before “seasoned prosecutors” could tell them “what was necessary to capture in order to prove a misdemeanor sex crime.”

That scandal erupted just days after it was revealed that Vance returned a $31,000 political contribution from President Trump’s personal lawyer in response to questions from reporters about his decision to drop a real estate fraud investigation involving the Trump SoHo development in 2012.

Vance, a Democrat, was re-elected without opposition to a third term Tuesday.

Weinstein has denied engaging in “non-consensual sex” with any of his accusers.