As "Marvel’s Agents of S.H.I.E.L.D." heads into Season 6 on May 10, the cast discussed their most memorable moments over the years. The characters in the series have been through many ups and downs, and the answers brought about both nostalgic and emotional moments.

For Tancharoen, how her own life intertwined with the show was the most meaningful. “For Jed and I, it was our first show together, and it was sort of like our first child,” she revealed. “We were very precious about it. We started Season 1 and became a family with all these people, and then we started our own family. For me, the birth of my daughter during the show was a wonderful moment for me.”

That familial feeling was key for Bell as well, as he looked all the way back to Season 1 when Clark Gregg was on set for the final shot. “It was about 10 o’clock at night,” remembered Bell, “And all the actors, who didn’t have to be there, all came back. We wrapped the season together. That kind of camaraderie has carried over for all these years.”

That commitment is a huge part of the fandom too; the dedicated “Marvel’s Agents of S.H.I.E.L.D.” fans are why we have Agent Phil Coulson today. “To have people at cons and online, say, ‘No. Coulson Lives.’ And here we are seven years later,” Gregg explained as his most memorable. And the family atmosphere wasn't lost on him either: “To grow so close with this incredible bunch of people. To have that experience, and to have that be birthed by a connection the fans had with a character who I think represents them, is probably the greatest thing that will ever happen to me.”

Some of Natalia Cordova-Buckley’s favorite moments were also due to the series’ fans. “I had never been on a show with this kind of fandom—an external family to our internal family,” she said. “It’s been really special to be a part of something that has such a following, and such support and love. Meeting [all] the fans is a really wonderful memory.”

The first time on set was a key moment for Henry Simmons. “Quite honestly, I can’t say it’s one scene or one episode because it just keeps getting better and better,” he reflected. “I love what [the writers] keep giving me, the wonderful experiences keep outmatching themselves.”

And Jeff Ward agreed that his first day on set was incredibly special. “I remember thinking, ‘this is a fun crew'," he explained. "When I got the call that they wanted to make me a series regular, it was very surreal. I didn’t think that was going to happen.”

What Elizabeth Henstridge remembered most is being able to work with an icon. “Getting to do a scene with Stan Lee. It was wonderful and crazy, and something that was beyond special.”