Embattled One Nation senator Rod Culleton has given the strongest indication yet he will quit Pauline Hanson's One Nation party if he survives a High Court challenge to his eligibility to serve in Parliament.

He said the party was not performing; had left farmers "bitterly disappointed"; had a toxic culture, and that he was evaluating his future, regardless of the High Court decision.

"This is about considering my position now. The real culture is starting to come through and it's not a culture that I'm in favour of," Senator Culleton told Fairfax Media in his Parliament House office.

"There are certain people in the party that I just don't think I could ever work with, to be honest."