Miniature Exercise Device (MED-2): The crew set up cameras in Node 3 and Cupola to capture video from multiple views of the Advanced Resistive Exercise Device (ARED) and MED-2 hardware, applied body markers, performed exercises and transferred video for downlink. The microgravity environment of space weakens muscle and bone, so orbiting crew members spend significant amounts of time exercising with equipment that is large and bulky. MED-2 aims to demonstrate that small robotic actuators can provide motion and resistance for crew workout sessions during long-duration space missions with exercise equipment that is smaller in size and mass that equipment currently being used on the ISS.

ADvanced Space Experiment Processor (ADSEP): The crew will removed Cell Culturing (CellCult) cassettes from ADSEP, inserted cells into each cassette and reinserted them into ADSEP. ADSEP is a thermally controlled facility that accommodates up to three cassette-based experiments that can be independently operated. A collection of experiment cassettes are used to accommodate experiments in cell technology, multiphase fluids, solution chemistry, separation science, microencapsulation, and crystal growth. For CellCult investigations, each cassette contains a rotating filtered bioreactor, a reservoir for fresh media, two peristaltic pumps, a waste reservoir, and up to 6 sample-collection or reagent containers connected by a manifold to the reactor. Cultures can be operated in continuous perfusion, batch fed, static, or sampling modes. The removal of samples and the addition of additives to the reactor volume can be programmed or teleoperated.

Microbial Tracking-2 (MT-2): The crew collected saliva samples for the Microbial Tracking-2 investigation and placed them in a Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). After the samples are returned to Earth, a molecular analysis of the RNA and DNA will be conducted to identify the specific microbes that are present on ISS. MT-2 monitors the different types of microbes on ISS over a 1-year period and how they change over time.

Vascular Echo Ultrasound: With guidance from a ground expert, a crewmember performed an ultrasound of the femoral artery on their right leg after a one minute light leg exercise. The Canadian Space Agency (CSA) Vascular Echo investigation examines changes in blood vessels and the heart while the crew members are in space, and then upon their return to Earth. The results could provide insight into potential countermeasures to help maintain crew member health.

Agenzia Spaziale Italiana (ASI) Biomission: The crew deinstalled Biomission experiment containers from the Kubik 5 facility in the Columbus module, completing the fifth run. Each of the containers will be placed into a Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) in order to preserve the scientific samples. Kubik 5 supports Biomission investigations by providing a small controlled-temperature incubator / cooler for the study of biological samples in a microgravity environment. Kubik is equipped with removable inserts designed for self-contained, automatic experiments using seeds, cells, and small animals.

Lighting Effects: This morning a crewmember provided a sleep log entry for the Lighting Effects investigation. The light bulbs on the ISS are being replaced with a new system designed for improved crew health and wellness. Fluorescent bulbs are being replaced with solid-state light-emitting diodes (LEDs) that have adjustable intensity and color. Investigators will determine if the new lights improve crew circadian rhythms, sleep, and cognitive performance. Results from this investigation also have implications for people on Earth who use electric lights.

Today’s Planned Activities

All activities were completed unless otherwise noted.

Lighting Effects Sleep Log Entry – Subject

Columbus Circadian Rhythms items repack

Video for episode 5 of “Space Yura and Nura” cartoon

DUBRAVA. Observation and photography using Photo Equipment

Preparation of Reports for Roscosmos web site and social media

Recording Greetings Video

ECON-M. Observation and photography

Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Drain

Miniature Exercise Device Hardware Gather

Terminate discharge of the 3rd Orlan 825М Battery Pack (Stow in spares kit ФГБ1ПГО_2_221_1)

Soyuz 735 Angular Rate Integration Unit (БИУС) telemetry setup

JEM System Laptop Terminal Reboot

Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Drain Part 2

Teardown of charger [ЗУ-С] setup, start

Miniature Exercise Device ARED Session 1st Crew

Bone Densitometer Express Rack Relocation

JEM Camera Robot Activation

On MCC GO Demating TLM connectors from ЗУ-С

Health Maintenance System (HMS) OCT Setup

JEM Camera Robot Shooting

Teardown of battery charger [ЗУ-С] setup, end

Hardware prepack for return and disposal on Soyuz 735

Miniature Exercise Device ARED Session 2nd Crew

JEM Camera Robot Deactivation

Temperature and Humidity Control (THC) Intermodule Ventilation (IMV) Flow Measurement Survey

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

DAN. Experiment Session

Health Maintenance System (HMS) – OCT Exam

PROBIOVIT. Insertion of Probiotic kit No.1 in Glovebox-S and turning the light on

Health Maintenance System (HMS) OCT Stow

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

Crew Departure Preparations for Return to Earth

PROBIOVIT. Filling Kit No. 1 Water container from СРВК

In Flight Maintenance (IFM) Waste and Hygiene Compartment (WHC) Full Fill

Space Technology and Advanced Research Systems Locker Installation

PROBIOVIT. Photography of water filling of the container from [СРВК]

PROBIOVIT. Filling Kit No. 1 Product container in Glovebox-S

PROBIOVIT. Photography during filling in the Glovebox

In Flight Maintenance (IFM) Waste and Hygiene Compartment (WHC) Full Fill

PROBIOVIT. Kite retrieval from Glovebox-S, Probiotic No.1 kit transfer and setup in ТБУ-В No.02 at +37 deg C /

Verification of ИП-1 Flow Sensor Position

ADSEP MELFI Removal #3

Crew Departure Preparations for Return to Earth

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

Advanced Space Experiment Processor Cell Collection

Filling (separation) of ЕДВ (КОВ) for Elektron or ЕДВ-СВ. Fill ЕДВ-КОВ cover No.1201 (00068432R, ФГБ1ПГО_1_110) from ЕДВ cover No.1296 (00071688R, СМ1ПрК)

Vascular Echo Exercise PDOP Measurement

PROBIOVIT. Setup of Probiotic kit No.2 in Glovebox-S

Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Fill Part 3

Environmental Health System (EHS) Grab Sample Container (GSC) Sampling Operations

PROBIOVIT. Filling Kit No.2 Water container from [СРВК]

PROBIOVIT. Filling Kit No. 1 Product container in Glovebox-S

Hardware prepack for return and disposal on Soyuz 735

PROBIOVIT. Kit retrieval from Glovebox-S, Probiotic No.2 kit transfer and setup in ТБУ-В No.2 at +37 deg C

PROBIOVIT. Photography of Probiotic kit No.2 in ТБУ-В No.02 at +37°С

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

PROBIOVIT. Dismantling Glovebox-S Hardware

Pre-pack cargo items for return or disposal on Soyuz 735

Probiotics Item Gathering

Experiment Container deinstallation

ADSEP MELFI Sample Insertion #2

ASI Experiment Container insertion into MELFI

Microbial Tracking-2 Saliva Setup

Health Maintenance System (HMS) Eye Exams

Completed Task List Activities

SABL2 Door Open – Morning

50S US Prepack

Ground Activities

All activities were completed unless otherwise noted.

ADSEP cell collection

THC IMV flow measure

Three-Day Look Ahead:

Friday, 08/25: Genes In Space, RR habitat restock, 50S prepack, WHC maintenance, Dragon transfer ops

Saturday, 08/26: Crew off duty, Mouse Habitat maintenance, ADSEP cell collection

Sunday, 08/27: Crew off duty, Marrow sampling

QUICK ISS Status – Environmental Control Group: