Airway Monitoring Measurement Operations: Using the Portable Pulmonary Function System (PPFS) for guidance, today two crewmembers performed two different measurement protocols; the low Nitric Oxide (NO) protocol which determines how much NO is exhaled with the respiration, and the high NO protocol, which determines how much NO is diffused into the blood. The protocols were initially performed in the Airlock at an ambient pressure and then repeated at a low pressure (10.2 psi). At the low pressure, oxygen concentration in the Airlock is increased to 27.5%. The primary goal of the Airway Monitoring experiment is to determine how gravity and microgravity influences the turnover of Nitric Oxide in the lungs. During future manned missions to the Moon and to Mars, airway inflammation due to toxic dust inhalation is a risk factor. Since dust may cause airway inflammation and since such inflammation can be monitored by exhaled Nitric Oxide analysis, the present study is highly relevant for astronaut health in future space programs. The US Airlock, for this experiment is used as a hypobaric facility for performing science. Utilizing the US Airlock will allow unique opportunities for the study of gravity, ambient pressure interactions, and their effect on the human body.

Arthrospira-B (Batch Culture) Assembly: The crew removed four Arthrospira experiment containers from the Biolab Incubator to exchange the reservoirs inside the Biolab. Following the exchange of the reservoirs, the ECs were reinstalled back onto Biolab Incubator. The Arthrospira B experiment is an important step in making improvements in the area of closed regenerative life support systems in space which will help in making future human exploration missions beyond low Earth orbit become a reality.

Human Research Program (HRP) Collections (Biochemical Profile, Repository): Today a 53S subject concluded his Flight Day (FD) 30 urine sample collection that began yesterday to support the Biochemical Profile and Repository experiments. The samples were placed in the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI).

Spaceflight-induced Hypoxic/ROS Signaling (APEX-05) Operations: The crew inserted two APEX-05 RNAlater Kennedy Space Center (KSC) Fixation Tubes (KFT)s into the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) prior to return on SpaceX-13 (SpX-13). The APEX-05 experiment grows different wild and mutant varieties of Arabidopsis thaliana, in order to understand how their genetic and molecular stress response systems work in space. The plants grow from seeds in the Veggie plant growth facility aboard the ISS, are frozen, and returned to Earth for detailed laboratory analysis.

Rodent Research 6 (RR-6) Transporter Setup: To prepare for the return of two of the four animal transporters on SpaceX-13, the crew reviewed reference material and participated in a conference about live animal return operations. The crew also set up and conducted checkout activities on the live animal transporter. The Rodent Research-6 (RR-6) mission uses mice flown aboard the International Space Station (ISS) and maintained on Earth to test drug delivery systems for combatting muscular breakdown in space or during disuse conditions. RR-6 includes several groups of mice selectively treated with a placebo or implanted with a nanochannel drug delivery chip that administers compounds meant to maintain muscle in low gravity/disuse conditions.

NanoRacks External Platform (NREP) Operations: Ground teams maneuvered the Japanese Experiment Module) Remote Manipulator System (JEMRMS) to transfer NREP from the Japanese Experiment Module Airlock (JEMAL) slide table to the Exposed Facility Unit (EFU)-4. After transfer operations were complete, the JEMRMS was maneuvered back to a stowed position and NREP was activated via ground command. NREP represents the first external commercial research capability for testing in support of scientific investigations, sensors, and electronic components in space.

Dragon Release On Board Training (OBT): This morning, the crew performed onboard training for this weekend’s upcoming release of the SpaceX-13 Dragon capsule. The training included the use of the Robotics On-Board Trainer (ROBoT) and a private conference with ground-based trainers.

Today’s Planned Activities

All activities are complete unless otherwise noted.



HRF Generic Urine Collection Male – Subject

Hematocrit Test

HRF Generic Sample MELFI Insertion Operations

Hematocrit Hardware Stowage

Regeneration of БМП Ф2 Micropurification Cartridge (start)

ISS CREW/SSIPC CONFERENCE

AIRMON Power On

On-Board Training (OBT) Dragon Release Review

Verification of ИП-1 Flow Sensor Position

Onboard Training (OBT) Dragon Release Conference

Manufacturing Device ABS Print Removal, Clean and Stow

СОЖ maintenance

Replacement of SSС2 and RSE-1 laptop network cable connectors.

LIOH and PFA Setup

On-board Training (OBT) Dragon Robotics Onboard Trainer (ROBoT) Release

24-hour ECG Recording (termination)

AIRMON preparation and First Low NO measurement

24-hour BP recording (terminate)

Prep for FGB СПОПТ ИДЭ-3 Smoke Detector needle cleaning (r/g review, equipment gathering).

Photo T/V (P/TV) Advanced Resistive Exercise Device (ARED) Exercise Video Setup

AIRMON Calibrations, Low NO and High NO measurements

Arthrospira EC Reservoir Exchange

Dust collectors ПC1, ПС2 filter cleaning by vacuum cleaner in FGB

24-hour ECG Recording (start)

Photo/TV Camcorder Setup Verification

AIRMON High NO measurement

Rodent Research Live Animal Return Review

Rodent Research Crew Conference

Polar Transfer To Dragon Overview Review

Preparations for and depressurization- Airway Monitoring

Polar Sample Transfer From MELFI

Comm Subsystem [СТТС] routine maintenance ops (LF path and VHF receiver test). Headset Audit.

AIRMON Low NO measurement at low pressure

TangoLab-2 Card Cube Remove

HRF Generic Urine Collection Stow

USB Jumpdrive Return and PPS Reconfiguration

AIRMON Low & High measurements at reduced pressure

Rodent Research Transporter Setup

Crew Wait for other crew

AIRMON High NO measurement at reduced pressure

Countermeasures System (CMS) VELO Exercise session Day 1

Airlock repressurization

Public Affairs Office (PAO) Event in High Definition (HD) – JEM

Dragon Cable Prep

VHF2d test from SM using primary СТТС sets

Power cycle LAB AVN443 HD Encoder

КСПЭ Camcorder setup and configuring in MRM2 (for mpeg2 TV coverage of High Gain Antenna boom translation)

TangoLab-1 Card Cube Remove

CONTENT. Experiment Ops

Acoustic Monitor Data Transfer and Stow

APEX-05 MELFI Sample Insertion #2

AIRMON Power Off

Micropurification Unit (БМП) Ф2 Absorption Cartridge (end)

Completed Task List Activities

EVA Procedure Review

Ground Activities

All activities are complete unless otherwise noted.



NREP transfer.

Standard commanding and payload support.

Three-Day Look Ahead:

Thursday, 01/11: Airways Monitoring, RR-6, Spheres Zero Robotics, POLAR Transfer, Petri Plate, Dragon cargo ops, Manufacturing Device Stow, Double Coldbag Pack Review

Friday, 01/12: Double Coldbag Pack, Dragon cargo ops, Dragon Unberth (Egress, N2 vestibule ops, depress, unberth), Lighting Effects, LMM Petri Plate, HRF fluid collections.

Saturday, 01/13: Dragon release, CUCU deact.

QUICK ISS Status – Environmental Control Group: