Roll Out Solar Array (ROSA): On Sunday, three attempts to retract ROSA solar array were unsuccessful. Imagery showed the array was not lining up correctly (“telescoping”), preventing the locking of ROSA’s latches which is required for return in Dragon. Teams met and agreed to stand down on further retraction attempts due to the risk of getting stuck in an off nominal configuration with a partially retracted array. ROSA was left fully deployed following the last attempt and jettison was approved which was completed today at 4:15PM CDT to the nadir and aft of ISS.

Seedling Growth 3: The crew completed the second of two six-day growth sessions for Seedling Growth 3 today. They removed Experiment Containers from the European Modular Cultivation System (EMCS), processed the samples, and stowed them in the Biolab Thermal Control Unit. The samples from this and the previous run will be returned on SpaceX-11. This is the third part of the Seedling Growth Experiment series using the small flowering plant Arabidopsis thaliana to investigate the effects of gravity on the cellular signaling mechanisms of light sensing in plants (phototropism), and to investigate cell growth and proliferation responses to light stimulation under microgravity conditions.

Microbial Tracking-2 (MT-2): Yesterday the crew collected saliva samples for the Microbial Tracking-2 investigation and placed them in a Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). MT-2 monitors the different types of microbes that are present on ISS over a 1 year period. After the samples are returned to Earth, a molecular analysis of the RNA and DNA will be conducted to identify the specific microbes that are present on ISS in order to understand the microbial flora diversity on the ISS and how it changes over time.

Human Research Program (HRP): A crewmember collected Flight Day 60 urine and blood samples yesterday and today for Biochem Profile and blood and air samples for Marrow.

The Biochem Profile experiment tests blood and urine samples obtained from astronauts before, during and after spaceflight. Specific proteins and chemicals in the samples are used as biomarkers, or indicators of health. Post-flight analysis yields a database of samples and test results which scientists can use in their study of the effects of spaceflight on the body.

The Marrow investigation identifies the effect of microgravity on bone marrow. It is believed that microgravity, like long-duration bed rest on Earth, has a negative effect on blood cell production in bone marrow.

Today’s Planned Activities

All activities were completed unless otherwise noted.

Test Video Recording for Russia Today TV Channel

Preparation of Reports for Roscosmos Web Site and Social Media

URAGAN. Observation and Photography

ECON-M. Observation and Photography

Marrow Breath And Ambient Air Sample Collection – Subject

Reminder HRF Generic Frozen Blood Collection

MO-8. Hardware setup

Reminder HRF Generic Urine Collection End

Body Mass Measurement – BMMD

HRF Generic Frozen Blood Collection Double Spin Collection – Subject

Body Mass Measurement – BMMD

Body Mass Measurement – BMMD

HRF Generic Frozen Blood Collection Double Spin Collection – Operator

MO-8 Concluding ops

HRF Generic Refrigerated Centrifuge Double Spin Configuration 1

HRF Generic Urine Collection Male – Subject

HRF Generic Sample MELFI Insertion Operations

HRF Generic Refrigerated Centrifuge Double Spin Configuration 2

HRF Generic Sample MELFI Insertion

HRF Generic Refrigerated Centrifuge Spin Conclude

Early unstow, priority, and US cargo transfers from Progress 436 (SM Aft) and IMS Ops

HRF Generic Frozen Blood Collection Conclude Stow

JEM Camera Robot Activation

HRF Generic Urine Collection Stow

Cargo Transfer to Dragon

Cell Biology Experiment Facility (CBEF) and Video Compression and Recording Unit 2 (VRU2) Cable Reconfiguration 2

Examination of bio electrical activity of heart at rest

Multipurpose Small Payload Rack (MSPR) Experiment Laptop Terminal (ELT) Cables Connection

Seedling Growth-3 Maintenance Work Area Preparation.

Removal of 800А (А105) battery

Multipurpose Small Payload Rack (MSPR) Experiment Laptop Terminal (ELT) RS485 Converter Connection

Seedling Growth-3 Maintenance Work Area Configuration.

JEM Camera Robot Deactivation

Dragon Locker Remove 2

Removal of 800А (А101) battery

Re-install 800А battery from А105 slot to А101 slot

Seedling Growth 3 TCU removal

Advanced Resistive Exercise Device (ARED) Cylinder Flywheel Evacuation

Countermeasures System (CMS) Treadmill 2 System (T2) Monthly Inspection

SPHERES USB Stow

EML Gas Valves Opening

Collecting SM and FGB Air Samples Using АК-1М Sampler

Seedling Growth-3 Camcorder Setup

Seedling Growth-3 Run 2 EC Removal from EMCS Rotor B

Request to RS1 with variable

Seedling Growth-3 Sample Processing of ECs Rotor B Run 2

Seedling Growth-3 Run 2 EC Removal from EMCS Rotor A

Seedling Growth-3 EMCS Cold Stowage Bag MELFI Insertion

Seedling Growth-3 Sample Processing of ECs from Rotor A Run 2

Seedling Growth-3 Sample Processing with FixBox Rotor A Run 2

Tightening of DC1 – Progress 435 QD screw clamps

Seedling Growth-3 EMCS Reference Experiment Container Insertion

Telescience Resource Kit Laptop Setup

Environmental Health System (EHS) – Surface Sampler Kit (SSK) and Microbial Air Sampler (MAS) Analysis T + 5

Dragon Cargo Operations Conference

Seedling Growth 3 TCU insertion

Seedling Growth-3 Post-Sample Processing Cleanup after Run 2.

Evening Preparation Work

Habitability Narrated Task Video Setup – Subject

Seedling Growth-3 MWA Deconfigure.

Habitability Narrated Task Video End – Subject

Completed Task List Items

Columbus PK4 Hard Drive Pouch Trash [Completed GMT 176]

Crew Medical Officer (CMO) Proficiency Training [Completed GMT 176]

Empty three Double Cold bags and prepare them for SpX-11 return [Completed GMT 176]

Ground Activities

All activities were completed unless otherwise noted.

P1UPOB ETVCG troubleshooting

Seedling Growth 3 Ops

Three-Day Look Ahead:

Tuesday, 06/27: StemCell media changeout, OBT CMO, Dragon LiOH filter bag R&R

Wednesday, 06/28: ELF sample holder exchange, sample transfer from MELFI to POLAR, WHC pre-treat tank R&R, Rodent Habitat restock

Thursday, 06/29: NanoRacks Module 48 ops, Rodent Research, Dragon departure OBT

QUICK ISS Status – Environmental Control Group: