GARY Ablett and Scott Pendlebury will measure their fitness levels against some of the world's biggest sports stars in the United States next week.

The two AFL guns will take a one-week break from their pre-season schedules to put their physical capabilities under the microscope for sports drink giant Gatorade at their world-leading IMG academy in Florida.

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The tour, which has been fully endorsed by the AFL and their clubs, will also include corporate box seats at the NFL Super Bowl and an NBA game.

Joined by Collingwood fitness chief Bill Davoren, Gold Coast's Ablett and Collingwood's Pendlebury will undergo rigorous testing over two days, designed to help the company's research into athletes' hydration needs.

Hoping @carmeloanthony puts on a show like this for me and @GaryAblettJnr next week when I'm over watching them play the Heat — Scott Pendlebury (@SP_10) January 25, 2014

Their results are then compared against some of the biggest names in world sport, such as NFL quarterback Eli Manning, LA Lakers Kobe Bryant and tennis champion Serena Williams.

Strategic Management boss Liam Pickering, who manages Ablett, 29, and Pendlebury, 25, said he expected their fitness levels to compare favourably with other sporting giants.

"It will be interesting to see when they are being compared to guys like (sprint king) Usain Bolt and I think the Brazilian soccer team," Pickering said.

"I think it will show for sure that they are right at the very top," Pickering said.

Ablett and Pendlebury are ambassadors for Gatorade.