Residents whose homes will be badly affected by a new bridge unlocking one of Melbourne's worst traffic chokepoints say the Andrews government is trying to turn their fight to protect their houses into a battle between them and Guide Dogs Victoria.

Guide Dogs Victoria's headquarters have been in Kew, next to the Yarra River, since 1962.

Residents protest VicRoads' plans for the Chandler Highway bridge. Credit:James Boddington

More than 200 properties stand on the other side of the river, in a small pocket of Alphington next to the busy Chandler Highway.

Between them lies the 124-year-old, two-lane Chandler Highway Bridge which VicRoads wants to be expanded to six lanes to resolve the traffic bottleneck.