Three men have been charged with the murder of missing Melton mother-of-two Simone Quinlan.

Melton men Wayne Marmo, 24, Brendan Neil, 28, and Dylan Jez, 23, are accused of killing the 33-year-old at Bacchus Marsh on August 25.

Simone Quinlan.

Mr Jez and Mr Marmo appeared at the Melbourne's Magistrate's Court on Saturday morning. Mr Neil requested not to appear.

Mr Marmo, wearing a black top and sporting a dark beard, was using crutches after his femur was broken in a car accident on August 31, the court was told.