Yes! Just drag your file over the input box and drop it. CyberChef can handle files up to around 2GB (depending on your browser), however some of the operations may take a very long time to run over this much data. If the output is larger than a certain threshold (default 1MiB), it will be presented to you as a file available for download. Slices of the file can be viewed in the output if you need to inspect them.

Maybe you have 10 timestamps that you want to parse or 16 encoded strings that all have the same key. The 'Fork' operation (found in the 'Flow control' category) splits up the input line by line and runs all subsequent operations on each line separately. Each output is then displayed on a separate line. These delimiters can be changed, so if your inputs are separated by commas, you can change the split delimiter to a comma instead. Click here for an example.