Background Retinol is used to reduce symptoms of skin aging. It affects surface lipids and increases skin regeneration ability.

Aim The aim of our study was to investigate the effect of retinol peel on the face and neck skin lipids in women, aged 50–69.

Material and methods The level of secreted sebum was measured using Sebumeter SM15 (Courage & Khazaka, Germany) on the forehead, cheeks, nose, chin, and neck. The measurements were carried out before each of the 3 retinol peel treatments applied at 3‐week interval and 3 weeks after the last treatment.

Results A statistically significant increase of lipid film in both U‐zone and T‐zone and on the neck was observed in the study group.