The Bourke Street Mall in Melbourne's CBD has been re-opened, after police responded to reports of a man moving along building awnings on the south side of the mall.

Police said the man was assisted off the roof and has been taken to hospital for a health check.

A section of the mall from Elizabeth Street to Little Collins Street was closed, and a number of fire trucks, ambulances and police officers were on the scene.

Yarra Trams said that trams 86 and 96 running along Bourke Street, and trams 55, 57, 59 and 19 on Elizabeth Street have resumed.

Police negotiate with a man on a building awning in Bourke Street Mall. ( Twitter: Bec F )

Police and firefighters on the scene at Bourke Street Mall. ( ABC News: Patrick Rocca )

Bourke Street Mall has been closed to trams and pedestrians. ( ABC News: Patrick Rocca )