Australian Anna Meares has again proved she is the queen of track cycling, breaking her own 500-metre time trial world record.

Meares became the first woman to go under 33 seconds for the event on Saturday when she made the most of the high altitude at the Mexico World Cup round.

Anna Meares. Credit:Reuters

The 30-year-old clocked 32.836 seconds to break her own mark of 33.010, set at last year's world titles in Melbourne.

She led an outstanding Australian performance on day two of the World Cup round, with Matthew Glaetzer scoring a breakthrough gold medal in the men's sprint.