CHARLOTTE, N.C. — Wine turned to water, Technicolor faded to black-and-white and the carriage assumed its former state as a mere pumpkin: The University of Maryland, Baltimore County, lost its second-round N.C.A.A. tournament game on Sunday to Kansas State, 50-43.

Thanks to U.M.B.C., No. 16 seeds’ records against No. 1 seeds in the Division I men’s tournament is now 1-135. Against No. 9 seeds, their record is now 0-1.

Having become the first No. 16 seed ever to defeat a No. 1 when they beat top overall seed Virginia on Friday, the Retrievers, which had won the America East Conference title, succumbed to a squad that had been no slouch itself. Kansas State had finished fourth in college basketball’s best conference, the Big 12. The Wildcats were athletic and big, with a defense U.M.B.C.’s Daniel Akin described as “more intense than Virginia’s.”

The Wildcats were also very aware of their place in this tale.

“We know that everyone is kind of looking for that Cinderella story,” Wildcats guard Barry Brown Jr. acknowledged a day before scoring a game-high 18 points to close the book on that story.