OsteoOmics Investigation: In preparation for OsteoOmic investigation activities planned this week, the crew configured OsteoOmics hardware and the Microgravity Science Glovebox (MSG), reviewed procedures, conferred with ground experts and completed an OsteoOmics practice session. Samples were retrieved from Minus Eighty Laboratory Freezer for ISS (MELFI) and General Laboratory Active Cryogenic ISS Experiment Refrigerator (GLACIER) for the investigation and temporarily stowed them for tomorrow’s operations. Crewmembers experience bone loss in orbit due to lack of gravity acting on their bones. OsteoOmics investigates the molecular mechanisms that dictate this bone loss by examining osteoblasts, which form bone, and osteoclasts, which dissolve bone. Improved understanding of these mechanisms could lead to more effective countermeasures to prevent bone loss during space missions and in a wide range of disorders on Earth. This may lead to better preventative care or therapeutic treatments for people suffering bone loss as a result of bone diseases like osteopenia and osteoporosis, or for patients on prolonged bed rest.

Japanese Experiment Module (JEM) Airlock (JEMAL) Operations: In preparation for the deploy of NanoRack Cubesat Deployer (NRCSD) #11 satellites planned for May 15, the crew opened the inner hatch of the JEMAL, extended the airlock Slide Table (ST) into the JEM Pressurized Module (JPM), mounted the Small Fine Arm Attachment Mechanism (SAM) and Multi-Purpose Experiment Platform (MPEP) facilities to the ST and returned the table into the JEMAL.

Human Research Program (HRP) Collections and Operations: A 50S crewmember collected Flight Day (FD) 15 blood and urine samples yesterday and today for Biochem Profile, Repository, and Marrow investigations and air samples for Marrow.

The Biochem Profile experiment tests blood and urine samples obtained from astronauts before, during and after spaceflight. Specific proteins and chemicals in the samples are used as biomarkers, or indicators of health. Post-flight analysis yields a database of samples and test results, which scientists can use to study the effects of spaceflight on the body.

Repository is a storage bank that is used to maintain biological specimens over extended periods of time under controlled conditions. This archive of biosamples will be used as a resource for future spaceflight related research.

The Marrow investigation identifies the effect of microgravity on bone marrow. It is believed that microgravity, like long-duration bed rest on Earth, has a negative effect on the bone marrow and the blood cells that are produced in the bone marrow.

SkinSuit Investigation: Over the weekend the crew performed two days of Skinsuit activities. With assistance from a crewmember acting as operator they took measurements after donning the suit in the morning and again before doffing in the afternoon. The Skinsuit is a tailor-made overall with a bi-directional weave specially designed to counteract the lack of gravity by squeezing the body from the shoulders to the feet, with a force similar to that felt on Earth. The subjects perform an evaluation of the efficacy of the Skinsuit in reducing or preventing lower back pain and preventing spine elongation. They measure the gravitational load provided by Skinsuit and evaluate operational considerations, in particular hygiene, microbiology, comfort, thermoregulation, donning and doffing, impingement and range of motion. Results from this investigation will be used to prepare for long duration missions.

Extravehicular Activity (EVA) Preparations: The EVA crew completed the following in preparation for the EXPRESS Pallet Controller Assembly (ExPCA) EVA currently planned for May 12.

Unstowed hardware from the Airlock not needed for the EVA and prepared the Airlock to support EVA operations.

Reviewed a briefing package including hardware overview/tool config, detailed timeline, and expected Caution and Warnings.

Compound Specific Analyzer-Combustion (CSA-CP) Checkout: The crew changed the batteries in 2 newly resupplied CSA-CP units and updated internal clock settings. The units will remain deactivated with new batteries for at least 24 hours for sensors to re-establish bias voltages.

Mobile Service System (MSS) Operations: Last Friday night, Robotics Ground Controllers powered up the MSS and used the Space Station Remote Manipulator System (SSRMS) and Special Purpose Dexterous Manipulator (SPDM) Arm 2 to extract the S11A-C Remote Power Control Module (RPCM). They temporarily stowed this RPCM in the S14B-H empty slot. Next, controllers extracted the S14B-G RPCM and installed it in the in S11A-C slot. Finally the RPCM in the S14B-H slot (old S11A-C) was extracted and installed in the S14B-G slot and the MSS was configured for park.

Cygnus Cargo Operations Status: The crew completed Message #2 over the weekend. Teams are working to complete Message #3 but at present, there are no cargo operations planned for this week.

Today’s Planned Activities

All activities were completed unless otherwise noted.

Marrow Breath And Ambient Air Sample Collection – Subject

Reminder HRF Generic Frozen Blood Collection Double Spin

Reminder HRF Generic Urine Collection End

HRF Generic Frozen Blood Collection Double Spin Collection – Subject

HRF Generic Refrigerated Centrifuge Double Spin Configuration 1

HRF Generic Urine Collection Male – Subject

HRF Generic Refrigerated Centrifuge Double Spin Configuration 2

HRF Generic Sample MELFI Insertion

Combustion Integrated Rack Alignment Guide Removal

HRF Generic Refrigerated Centrifuge Spin Conclude

HRF Generic Sample MELFI Insertion

Preventive Maintenance of FS1 Laptop (Cleaning and rebooting)

VIZIR. Start charging СКПИ Infrared Receiver (ИКП) battery

HRF Generic Frozen Blood Collection Conclude Stow

JEM Internal Port Camera HD Status Check

Atmospheric Control System (ACS) NORS Oxygen Transfer to Low Pressure O2 Tank

NAPOR-mini RSA. Cleaning Onboard Memory Storage БЗУ-М vents

Monthly Smart Switch Router (БРИ)

HRF Generic Urine Collection Stow

OsteoOmics SABL CO2 Valve Open

XF305 Camcorder Setup

Comm config to support tagup on S/G2 from MRM2

JEM Airlock Slide Table (ST) Extension to JPM Side

RSE-2 laptop install in MRM2 Test activation

Active Mechanism Tether Loop Removal from JEM Airlock Slide Table

Small Fine Arm (SFA) Airlock Attachment Mechanism (SAM) installation To JEM Airlock (AL) Slide Table

Extravehicular Activity (EVA) Airlock Unstow

Photo/TV Camcorder Setup Verification

OsteoOmics Bag Retrieval

MRM2 Comm Reconfig for Nominal Ops

OsteoOmics MWA Prep

EVA Safing for Thermal Painted Surfaces on ExPCA ORU

Extravehicular Activity (EVA) Tool Configuring

СКПФ1, СКПФ2 Dust Collector and MRM1 ГЖТ Cleaning with Vacuum Cleaner

JEM Airlock Capture Mechanism Release

Photo/TV Camcorder Setup Verification

JEMRMS Multi-Purpose Experiment Platform Installation to Small Fine Arm Attachment Mechanism Part1.

JEM Airlock Capture Mechanism Capturing

JEMRMS Multi-Purpose Experiment Platform Installation to Small Fine Arm Attachment Mechanism Part2.

Changeout of SM ПФ1-4 dust filter cartridge

JEM Airlock Slide Table (ST) Retraction from JPM Side

EVA Safing and Cleanup for Thermal Painted Surfaces on ExPCA ORU

CASKAD. Manual Mixing in Bioreactor

VIZIR. CКПИ Infrared Receiver battery charge

OsteoOmics Operations Practice

OsteoOmics Operations Review 1

ISS Crew Orientation

RS2 and RS3 laptops inspection and cleaning

OsteoOmics BioCell Split

OsteoOmics Payload Crew Conference

Auxiliary Laptop Computer System Virus Definition File Update

Environmental Health System (EHS) – Compound Specific Analyzer-Combustion (CSA-CP) Checkout

OsteoOmics MSG Setup

RS1 laptop inspection and cleaning

В3 fan grill cleaning in DC1

Public Affairs Office (PAO) High Definition (HD) Config for JEM Event

PAO Preparation

Public Affairs Office (PAO) Event in High Definition (HD) JEM

Crew Medical Officer (CMO) Proficiency Training

Exercise Data Downlink via OCA

Atmospheric Control System (ACS) Nitrogen Oxygen Recharge System(NORS) Oxygen Transfer Termination

VIZIR. CКПИ Infrared Receiver battery charge

SM Ventilation Subsystem Preventive Maintenance. SM Ventilation Subsystem Preventive Maintenance. Group В1

СОЖ (SOZH) maintenance

Combustion Integrated Rack Alignment Guide Installation

OsteoOmics MERLIN Removal

Completed Task List Items

EVA tool config

ESA PAO daily activities

N2P knob tightening

Everywear Smartshirt setup

HRF generic frozen blood collection double spin overview

VEG-03 consumption harvest #1

VEG-03 plant water/photo

Cygnus cargo ops

IFM Robotic Hand Controller

EMER CMS Battery Charging

JSL Router Cable T/S

ARED Slider Inspection

Extremo Session A

CD Library Audit

IFM Light Lab1Op2 R&R

Wanted Docking Accessory Kit

Ground Activities

All activities were completed unless otherwise noted.

OsteoOmics support

EVA prep support

Three-Day Look Ahead:

Tuesday, 05/02: OsteoOmics media change, EVA loop scrub, Fluidics

Wednesday, 05/03: OsteoOmics media change, Sprint Ultrasound, OBT ISS emergency hardware familiarization

Thursday, 05/04: OsteoOmics media change, EVA tool config/SAFER checkout, CEVIS portable PFS setup

QUICK ISS Status – Environmental Control Group: