FRANKFURT — The United States assailed the European Union on Wednesday for adding American territories to its “dirty money” list, opening a new rift in a relationship that has grown increasingly fractious amid disputes over trade and Iran sanctions.

Four United States territories, including Puerto Rico, were added to a money laundering blacklist that will require European banks to apply greater scrutiny to transactions in those regions. That prompted an angry rebuke from the Trump administration, which criticized the methodology used by the bloc to add those regions and said American officials were not consulted ahead of their inclusion.

The blacklist lumped Puerto Rico, American Samoa, Guam and the United States Virgin Islands with the likes of North Korea, Libya and Yemen as havens where drug dealers, terrorists and corrupt dictators can launder ill-gotten gains.

Saudi Arabia, which leads the Riyadh-based Terrorist Financing Targeting Center with the United States, was also added to the list despite its efforts to demonstrate that it is cracking down on illicit financial activity. That decision could further complicate Europe’s relations with the kingdom, which already faces scrutiny for its role in the murder of a dissident Saudi journalist, Jamal Khashoggi. Steven Mnuchin, the Treasury secretary, has made combating illicit financial activity a priority and has twice visited the center since it opened in 2017.