Amsterdam, February 10, 2016 - European honeybees are being poisoned with up to 57 different pesticides, according to new research published in the Journal of Chromatography A. A new method for detecting a whole range of pesticides in bees could help unravel the mystery behind the widespread decline of honeybees in recent years, and help develop an approach to saving them.

Honeybees are under threat globally: in the US, dramatic declines in bee populations due to a condition called colony collapse disorder (CCD) continues to put crops at risk an farmers out of business. Several studies have shown a link between pesticide use and bee deaths and the European Union has banned the use of neonicotinoid pesticides.

But it's not as simple as banning one pesticide that's killing bees; the relationship between pesticide use and bee death is complex and scientists are still trying to figure out exactly what's happening. In the new study, researchers from the National Veterinary Research Institute in Poland have developed a method for analyzing 200 pesticides at the same time, to figure out what's really putting honeybees at risk.

"Bee health is a matter of public concern -- bees are considered critically important for the environment and agriculture by pollinating more than 80% of crops and wild plants in Europe," said Tomasz Kiljanek, lead author of the study from the National Veterinary Research Institute in Poland. "We wanted to develop a test for a large number of pesticides currently approved for use in the European Union to see what is poisoning the bees."

With so many pesticides currently in use, it's difficult to work out which ones are harming the bees. Certain combinations of pesticides, or their use over time, could affect honeybees in different ways. In order to understand what's really going on, we need to know which pesticides and at what concentration levels are present in honeybees.

Kiljanek and the team used a method called QuEChERS, which is currently used to detect pesticides in food. With this analysis, they could test poisoned bees for 200 different pesticides simultaneously, as well as several additional compounds created when the pesticides are broken down. About 98% of the pesticides they tested for are approved for use in the European Union.

The team used the method to investigate more than 70 honeybee poisoning incidents. Their findings revealed 57 different pesticides present in the bees -- it's a toxic puzzle they hope their new method will help solve.

"This is just the beginning of our research on the impact of pesticides on honeybee health," said Kiljanek. "Honeybee poisoning incidents are the tip of the iceberg. Even at very low levels, pesticides can weaken bees' defense systems, allowing parasites or viruses to kill the colony. Our results will help expand our knowledge about the influence of pesticides on honeybee health, and will provide important information for other researchers to better assess the risk connected with the mix of current used pesticides."

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Article details

"Multi-residue method for the determination of pesticides and pesticide metabolites in honeybees by liquid and gas chromatography coupled with tandem mass spectrometry -- Honeybee poisoning incidents" by Tomasz Kiljanek, Alicja Niewiadowska, Stanis?aw Semeniuk, Marta Gawe? and Milena Borz?cka, Andrzej Posyniak (doi: 10.1016/j.chroma.2016.01.045). The article appears in Journal of Chromatography A, Volume 1435 (February 2016), published by Elsevier.

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About Journal of Chromatography A

The Journal of Chromatography A publishes original research and critical reviews on all aspects of fundamental and applied separation science. It covers chromatography and related techniques, electromigration techniques (for example, electrophoresis and electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Most research papers deal with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.

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