

This is how I have inoculated my jars for years. I have never run into any contam problems unless it was a bad source(Syringe, print, LC). I do this in open air, and it takes me about a minute to do a set of 12 jars.





Here are the materials needed. I am sure you can make everything out, the little orange square is a piece of sponge that I cut.







To start with, drop your piece of sponge into a container of alcohol.







For the next step, simply flame your needle, and insert it into the sponge.







Finally, push through your inoculation point, squirt however many cc's you wish to use, and pull the needle back out.







I hate the time it use to take me inoculating jars. I do this in open air right on my kitchen table. No need to mess with the tight quarters of a glove box, or building a flow hood. This process goes very quickly if you have a self healing lid. If you do not, then I use to just cover up the inoculation hole with a small band-aid. Instead of pulling the sponge off the top of the lid, along with the needle, just pull the needle out of the sponge, leaving the sponge to cover the whole while you reach for your band-aid or whatever you use.



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Self Healing lid tek ** Update 10.17.17 **

Mini casing pictures: Pins to harvest

Cup O' Shrooms

Magash: I noticed my contams were in the shape of fingers

Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger.



My final Grow!





Edited by Citric (07/07/09 05:35 PM)



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Quote:

Citric said:

Quote:

Lennyk said:

That is cool, although it has been done before in one form or another, like agar did it for one.









I posted it years ago as well, but never included pictures or nothing. Figured I'd make a post with one.







Sorry to mess up the quote, I changed what I said, but anyway I like the method for how simple it is, but I found it too risky if one didn't have self healing ports. It ended up having problems even though there was only a split moment of being exposed.



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do you just use rvt silicon for the ports?



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Quote:

Lennyk said:

I like the method for how simple it is, but I found it too risky if one didn't have self healing ports. It ended up having problems even though there was only a split moment of being exposed.







I have done a lot of jars before I used the silicone port. Rather then keeping the needle inside the sponge, I would hold the sponge on top of the lid, get the band-aid, then move and place it on. There was maybe a 1/2 second that went by that the 'hole' was exposed. Never ran into issues.



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Self Healing lid tek ** Update 10.17.17 **

Mini casing pictures: Pins to harvest

Cup O' Shrooms

Magash: I noticed my contams were in the shape of fingers

Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger.



My final Grow!





Edited by Citric (07/07/09 05:36 PM)



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Quote:

Citric said:

Quote:

Lennyk said:

I like the method for how simple it is, but I found it too risky if one didn't have self healing ports. It ended up having problems even though there was only a split moment of being exposed.







I have done a lot of jars before I used the silicone port. Rather then keeping the needle inside the sponge, I would hold the sponge on top of the lid, get the band-aid, then move and place it on. There was maybe a 1/2 second that went by that the 'hole' was exposed. Never ran into issues.









Maybe my house just sucks, never had good luck with open air outside the first pf attempt which went fine, everything open outside a glovebox always gets too iffy, although the method is pretty sound.



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A sponge of course! Great idea !



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Fine line between fishing, and standing on the shore with a pole in your hand.



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Quote:

Lennyk said:

Quote:

Citric said:

Quote:

Lennyk said:

I like the method for how simple it is, but I found it too risky if one didn't have self healing ports. It ended up having problems even though there was only a split moment of being exposed.







I have done a lot of jars before I used the silicone port. Rather then keeping the needle inside the sponge, I would hold the sponge on top of the lid, get the band-aid, then move and place it on. There was maybe a 1/2 second that went by that the 'hole' was exposed. Never ran into issues.









Maybe my house just sucks, never had good luck with open air outside the first pf attempt which went fine, everything open outside a glovebox always gets too iffy, although the method is pretty sound.









That sucks!



Why not make some self healing lids? I use to hate inoculating without them, it was so tedious!



--------------------

Self Healing lid tek ** Update 10.17.17 **

Mini casing pictures: Pins to harvest

Cup O' Shrooms

Magash: I noticed my contams were in the shape of fingers

Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger.



My final Grow!





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Quote:

Lennyk said:

Maybe my house just sucks, never had good luck with open air outside the first pf attempt which went fine, everything open outside a glovebox always gets too iffy, although the method is pretty sound.







Yeah... Your method is great! Living in Hawaii, it's a perfect temp for active mold. I've always had contam problems with my inocs (close to 90% of all jars) if I inoced with just the still air method. I always needed a hepa filter to get those things out.



But your method really saves money. So far I've not have had no contams with your method. So for those who can't really afford a blower/hepa filter setup, and their environment makes their contam rate high, this is a great method.



Edited by ccapistrano (07/07/09 06:18 PM)



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I inoculate almost the same way except I use those disposable alcohol wipes.



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Oh, welcome to this world of fools

Of pink champagne and swimming pools





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how long does it take for the rvt to dry



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nice and simple, fool proof



i never messed with silicon yet, must give it a go sometime.



micropore tape and gloves is troublesome.



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Quote:

proudabu said:

how long does it take for the rvt to dry







you can pc it after about 30min(once it is dry enough not to stick to the foil) it will cure in the pc.



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Oh, welcome to this world of fools

Of pink champagne and swimming pools





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I'm going to give this method a shot. Sounds fantastic. Would also like to mention that you can boil the sponge to kill off anything else that may be residing on it before you soak it in alcohol.



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My first harvest:







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