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Donald J. Trump‘s sniffles have returned.

During the second presidential debate, social media users pointed out the Republican nominee appeared to sniffle loudly as he and Democratic nominee Hillary Clinton squared-off on a number of explosive topics that included the political fallout following the release of a video tape that shows him making obscene and sexually aggressive comments about women.

WATCH: Donald Trump responds to leaked tape calling it just “locker room talk”

1:56 Presidential debate: Donald Trump responds to leaked tape calling it just “locker room talk” Presidential debate: Donald Trump responds to leaked tape calling it just “locker room talk”

Trump’s sniffling was a popular topic on social media during the first debate and sparked a new round of jokes on Sunday, with many using the hashtag #Sniffle on Twitter.

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Sniff… sniff… — Al Giordano (@AlGiordano) October 10, 2016

Is every sniff a lie? #debate — Maureen Johnson (@maureenjohnson) October 10, 2016

Looks like Trumps mic is broken again? #sniffle #Debates2016 — Carmen Angelica (@Carmesancheeses) October 10, 2016

Obamacare can help that #sniffle — Joey (@oxkidox) October 10, 2016

It's locker room sniffing — Keith Olbermann (@KeithOlbermann) October 10, 2016

The billionaire businessman also sniffed as he responded to questions about the leaked 2005 video showing Trump bragging to Access Hollywood host Billy Bush about sexually assaulting women.

Trump’s heavy breathing became a hot topic in the previous debate and when he was asked about it the next morning, Trump denied he’d been sniffling.

“No, no sniffles,” he said on “Fox & Friends.” ”No sniffles, no cold.” Tweet This

The fiery second presidential debate began with Hillary Clinton and Donald Trump refusing to shake hands as they entered the debate hall in St. Louis.

The two candidates exchanged several blows on the Trump tapes, the Clinton email scandal and the U.S. affordable care act.