Prime Minister Malcolm Turnbull has called on independent MP Bob Katter to withdraw a controversial campaign ad apparently depicting him shooting his rivals.

The ad, posted online yesterday, shows two people wearing ALP and LNP shirts erecting an Australia "for sale" sign.

Mr Katter is then seen with a smoking gun and the two people lying on the ground behind him.

Mr Turnbull said Mr Katter should apologise.

"The advertisements were in the worst of taste and Mr Katter should apologise and withdraw them," he said.

Mr Katter has also faced a call to step down over the advertisement from LNP Senator Barry O'Sullivan, who said it was "abhorrent".

"You know, the time's up," Mr O'Sullivan said.

"Bob's done some crazy things over the years, but all of that pales into insignificance when he starts to promote the use of violence to murder unarmed political opponents and then declares his defence as he was having a bit of fun."

Mr Katter's gay half-brother said the video was highly inappropriate, given the recent mass shooting at the Pulse nightclub in Orlando.

"It's a total disregard for the loss of lives that we saw in Orlando recently, which is still having a huge impact on my community which is the LBGTI, but also the greater community," Carl Katter, the ALP's candidate in the Melbourne seat of Higgins, said.

Sorry, this video has expired Bob Katter defends gun advertisement

But Bob Katter told Channel Seven's Sunrise program this morning he did not have time to pay attention to the media headlines.

"I get in every night at midnight, and with all due respect, I watch a movie if I switch the television on," he said.

"I do not read the newspapers, I haven't read the newspapers in ages."

Yesterday, he told the ABC that he signed off on the video over a week ago and that he had "no idea when the thing went out".

"It most certainly wasn't under my control," he said.

Video 'just for humour value'

Carl Katter also said the advertisement did not reflect Australia's attitude to guns.

"Australians, they're very proud that we've taken [our] stance for gun laws, but to see this in our country is gross," he said.

"It's disgusting and I think a lot of people, everyone I've spoken to, finds it just disgusting."

But Mr Katter said that he was not being deliberately controversial.

"I think most people will enjoy the humour. I thought it was very funny, I must admit," he told the ABC.

"It was just for humour value. But it was humour getting a message across.

"What's not funny is the selling off of this country. We hate it and we're tenaciously opposed to it."

Mr Katter said the advertisement was "our idea" but that his party had help from satirical newspaper Betoota Advocate.

"Maybe you shouldn't be waving guns around several days after a horrific mass murder," one Twitter user wrote yesterday.

"About time a politician saw sense. Keep up the good work Bob," a Facebook user wrote.

Labor senator Penny Wong told 612 ABC Brisbane yesterday said she was "always uncomfortable with political ads which choose to, even in a satirical sense, make fun of violence".