The wife of retired Rangers legend Brian Leetch and her sister, Tracy Murphy, briefly appeared in court Monday to face charges that they roughed up a bartender and two others on a ladies’ night out in Manhattan.

In a new allegation leveled against Murphy, 44, prosecutors accuse her of kneeing the barkeep in the groin during the alleged May 12 hair-pulling, nail-scratching melee.

The brawl at Brandy’s Piano Bar on East 84th Street erupted after Mary Beth Leetch, 49, Murphy and another pal were asked to leave for singing too loudly, according to court papers.

Mary Beth allegedly charged at a female staffer, who was trying to record her, and yanked her hair while Murphy twisted her arm and scratched her.

The feisty duo set on the bartender next, ripping his shirt, hitting him on the head and leaving him with a gash on his forehead, the criminal complaint states.

The third victim was allegedly hit in the face when he tried to intervene.

The defendants are due back in court Nov. 7.