Vegan protesters responsible for bringing Melbourne's CBD to a standstill earlier this year have been stripped of their right to claim federal tax concessions.

The Australian Charities and Not-for-profits Commission (ACNC) stripped Aussie Farms of its charitable status late on Monday.

Vegan protesters in Melbourne earlier this year.

Aussie Farms registered as a charity in January 2018 and has since been the subject of intense criticism, including from federal ministers, for its online map and directory of slaughter houses and farms across Australia. Agriculture Minister David Littleproud led the attack against the group, slamming the directory as an "attack map" designed to encourage vegan vigilantism.

Commissioner Gary Johns said the commission revoked an organisation's charitable status only in the "most serious of cases ". He cited media coverage of the organisation's protests and break-ins at farms and abattoirs but said the commission's reasons for its ruling would remain secret.