Jameis Winston has filed counterclaims against the woman who accused him of rape, responding to her lawsuit Friday in federal court in Orlando, Fla., by denying the accusations she lodged last month and accusing her of defaming him and attempting to hinder his career.

The woman, Erica Kinsman, who identified herself in the recent documentary “The Hunting Ground,” said Winston raped her in December 2012. Winston was never questioned during an investigation by the police in Tallahassee, Fla., and local prosecutors declined to charge him.

At a hearing about the allegation earlier this year at Florida State, where Winston was a Heisman Trophy-winning quarterback, a former Florida Supreme Court chief justice found that the evidence did not show that Winston had violated the university’s code of conduct. The decision was sustained on appeal.

Winston, who led the Seminoles to the national championship in the 2013 season, was picked No. 1 by the Tampa Bay Buccaneers in this year’s N.F.L. draft and signed a four-year deal reportedly worth $23.35 million. His case has become a lightning rod in an already tense national discussion over sexual violence on campuses. Winston has maintained that his sexual encounter with Kinsman was consensual.