Since this question came up again today, I figure it was worth sharing in case others also had trouble using the vSphere Guest Operations API in VMware Cloud on AWS (VMC), which includes PowerCLI's Invoke-VMScript and Copy-VMGuestFile cmdlet. There are a couple of requirements that you must satisfy both in the GuestOS as well as between your on-prem vSphere environment and VMC.

VMware Tools installed and running, it may seem obvious, but I have had customers trying to use various scripts without realizing this was a requirement. You should also ensure that you are running the latest version of VMware Tools, especially as there bugfixes that may impact Guest Operations APIs. VPN or Direct Connect (DX) configured between your on-prem vSphere environment and VMC, this is required as you will need access to ESXi hosts which is only available through a VPN or DX Create a VMC firewall rule to allow access from your on-prem network to VMC's ESXi hosts on port 443 which is used for Guest Operations access including transferring files to and from the GuestOS



The VMC firewall rule is usually the thing that most folks forget about and this simply because for most on-prem environment, access to ESXi over 443 is just sort of a default.

Once you have configured the VMC firewall to allow 443 to ESXi hosts, you will be able to use the Guest Operations API including Invoke-VMScript and Copy-VMGuestFile to a VM running in VMC