Thursday a jury was shown a video timeline revealing Omar Mateen, 29, open fire at Pulse nightclub in Orlando, Florida in June 2016

Harrowing new footage of Pulse nightclub shooter Omar Mateen has been released, revealing the moment Omar walked past the front security guards and opened fire seconds later in the bar.

The graphic video timeline was played in court on Thursday by federal prosecutors in a case against Mateen's widow Noor Salman, who turned her head away from the monitors and began to cry.

Salman, 31, is accused of helping her husband carry out the mass shooting that killed 49 people and wounded 58 at the gay nightclub, and of lying to law enforcement afterwards.

The video, presented to a jury of 12, revealed surveillance footage of Mateen passing security guards, paying admission, and entering the club at 1:41am.

Just before 2am he leaves to move his rental van closer to the club. When he re-enters, he's armed with an automatic weapon, a Glock and a knife, immediately opening fire seconds later.

The surveillance video also sees Mateen reload his gun several times. At one point his semi-automatic jammed, but he had a hand gun holstered to his side.

The club's surveillance footage reveals Mateen reenter the club, pictured above passing the front desk, just before 2am armed with an automatic weapon, a Glock and a knife

Seconds after he passes the front desk Mateen opens fire in the main bar area, where victims began to immediately collapse or fall to the ground

The video footage at the bar saw him reload his weapon several times as he walked through the rooms of the gay nightclub

This is the moment police first entered the night club, passing the front desk at 2:09am

Police then made their way through the club looking for Mateen, pictured above at 2:11am

Police had their arms aimed at a door that led to the an upper level of the club at 2:47am

Helicopter footage also tracked the moment that police busted through the bathroom wall around 5:15am, where Mateen was hiding inside with hostages

The clip showed in court sees Mateen head to the bathroom where he fired at the stalls.

Victim Bobby Rodriguez was in that bathroom where she hid under a body draped on top of her.

Video footage recorded from her cellphone also played for the jury revealing her shallow breathing in the stall huddled on the floor.

'The noise of the gunshots was only matched by the moans and screams from the victims... One man who was badly injured rushed into the bathroom and crawled under her stall,' she said in court, according to the Orlando Sentinel.

When she saw the injured man, Rodriguez said she placed his body on top of her and 'played dead'.

Police arrived on the scene at 2:07am and are seen in the video entering the club. At that time Mateen was hiding in the bathroom with hostages, according to WFTV.

Helicopter footage also shows the moment police busted through the bathroom wall where they discovered Mateen and shot him around 5:15am, after a three-hour stand-off.

The jarring video was shown to show the jury the magnitude of the massacre.

A redacted version was released publicly Friday.

Prosecutors are trying to link Salman to her husband's shooting.

Noor Salman, the widow of Mateen, looked away from the monitors displaying the video in court and began to cry

Pictured in a brown ponytail, she turned her head away from the monitors that pictured Mateen reloading his gun and opening fire inside the nightclub

Pictured in court with her defense attorney Linda Moreno, she says she was home with the couple's three-year-old son during the time of the shooting

Prosecutors are accusing Salmon, pictured left with Mateen right, of knowing about her husband's plans for the shooting. If found guilty of aiding and abetting him, she could face life in prison

The case will depend on whether or not Salman knew of Mateen's plans for the June 12, 2016 shooting.

If she is found guilty of aiding and abetting Mateen, in addition to obstructing a federal investigation, she could face a lifetime prison sentence.

Prosecutors must first establish if the massacre was a terror act motivated by Islamic terrorism.

During the time of the shooting Salman was at home with the couple's three-year-old son.

On Wednesday during the trial prosecutors claimed Salman knew about Mateen's plans, claiming that she already admitted this to authorities.

Assistant US Attorney Jeff Mandolfo led the prosecution telling jurors that Salmon admitted to FBI she regretted not doing more and told the court that 'I'm sorry for what happened. I wish I wouldn't told'.

Salman initially told investigators that her husband acted without her knowledge.

However, she later acknowledged being aware that her husband was watching Islamic State recruitment videos.

He had also purchased an assault rifle and examined three possible attack locations, according to prosecutors.

Salman has pleaded not guilty.

The trial is expected to last for a month and will take place at the Orlando courthouse.