1 Pan Y

Zhang D

Yang P

Poon LLM

Wang Q Viral load of SARS-CoV-2 in clinical samples. 2 Ye G

Lin H

Chen L

et al. Environmental contamination of the SARS-CoV-2 in healthcare premises: an urgent call for protection for healthcare workers. We previously reported the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in different clinical samples.This virus can be detected on different surfaces in a contaminated site.Here, we report the stability of SARS-CoV-2 in different environmental conditions.

50 ] per mL) was incubated for up to 14 days and then tested for its infectivity ( We first measured the stability of SARS-CoV-2 at different temperatures. SARS-CoV-2 in virus transport medium (final concentration ∼6·8 log unit of 50% tissue culture infectious dose [TCID] per mL) was incubated for up to 14 days and then tested for its infectivity ( appendix p 1 ). The virus is highly stable at 4°C, but sensitive to heat. At 4°C, there was only around a 0·7 log-unit reduction of infectious titre on day 14. With the incubation temperature increased to 70°C, the time for virus inactivation was reduced to 5 mins.

50 per mL) was pipetted on a surface (2 per piece) and left at room temperature (22°C) with a relative humidity of around 65%. The inoculated objects retrieved at desired time-points were immediately soaked with 200 μL of virus transport medium for 30 mins to elute the virus. Therefore, this recovery of virus does not necessarily reflect the potential to pick up the virus from casual contact. No infectious virus could be recovered from printing and tissue papers after a 3-hour incubation, whereas no infectious virus could be detected from treated wood and cloth on day 2. By contrast, SARS-CoV-2 was more stable on smooth surfaces. No infectious virus could be detected from treated smooth surfaces on day 4 (glass and banknote) or day 7 (stainless steel and plastic). Strikingly, a detectable level of infectious virus could still be present on the outer layer of a surgical mask on day 7 (∼0·1% of the original inoculum). Interestingly, a biphasic decay of infectious SARS-CoV-2 could be found in samples recovered from these smooth surfaces ( 3 Chu DKW

Pan Y

Cheng SMS

et al. Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. We further investigated the stability of this virus on different surfaces. Briefly, a 5 μL droplet of virus culture (∼7·8 log unit of TCIDper mL) was pipetted on a surface ( appendix p 1 ; ∼cmper piece) and left at room temperature (22°C) with a relative humidity of around 65%. The inoculated objects retrieved at desired time-points were immediately soaked with 200 μL of virus transport medium for 30 mins to elute the virus. Therefore, this recovery of virus does not necessarily reflect the potential to pick up the virus from casual contact. No infectious virus could be recovered from printing and tissue papers after a 3-hour incubation, whereas no infectious virus could be detected from treated wood and cloth on day 2. By contrast, SARS-CoV-2 was more stable on smooth surfaces. No infectious virus could be detected from treated smooth surfaces on day 4 (glass and banknote) or day 7 (stainless steel and plastic). Strikingly, a detectable level of infectious virus could still be present on the outer layer of a surgical mask on day 7 (∼0·1% of the original inoculum). Interestingly, a biphasic decay of infectious SARS-CoV-2 could be found in samples recovered from these smooth surfaces ( appendix pp 2–7 ). 39 representative non-infectious samples tested positive by RT-PCR(data not shown), showing that non-infectious viruses could still be recovered by the eluents.

50 per mL) to 135 μL of various disinfectants at working concentration ( 4 van Doremalen N

Bushmaker T

Morris DH

et al. Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. We also tested the virucidal effects of disinfectants by adding 15 μL of SARS-CoV-2 culture (∼7·8 log unit of TCIDper mL) to 135 μL of various disinfectants at working concentration ( appendix p 1 ). With the exception of a 5-min incubation with hand soap, no infectious virus could be detected after a 5-min incubation at room temperature (22°C). Additionally, we also found that SARS-CoV-2 is extremely stable in a wide range of pH values at room temperature (pH 3–10; appendix p 1 ). Overall, SARS-CoV-2 can be highly stable in a favourable environment,but it is also susceptible to standard disinfection methods.

This work was supported by National Institute of Allergy and Infectious Diseases, National Institutes of Health (contract HHSN272201400006C ). LLMP was supported by the Croucher Foundation. We declare no competing interests.

Supplementary Material Supplementary appendix