Uniformed police marched in Brisbane's annual Pride Festival parade for the first time in its 25-year history after Commissioner Ian Stewart lifted an earlier ban.

Hundreds of people gathered in Fortitude Valley on Saturday morning wearing a multitude of costumes and rainbow colours to support Queensland's lesbian, gay, bisexual, transgender, intersex and queer (LGBTIQ) community.

Organisers said a record crowd of 1,500 took part in the march in Brisbane, with Dykes on Bikes leading the parade from Fortitude Valley down to the rainbow planted arbour of New Farm Park.

Paramedics in uniform also marched in support of the festival, set up to oppose any prejudice, discrimination and stigma against the LGBTIQ community.

Queensland Police Constable Reis Maher said it had been great to march and be in uniform.

"I marched down in Mardi Gras last year with my partner, who was in the Navy at the time, which was a fantastic experience," he said.

"While I was there I really thought it would be really, really great to have to be able to march in my uniform one day ... and then 2015, and we are."

Queensland Ambulance Service worker Drew Hebbron joined colleagues at the parade.

"There's over 50 of us here, which is incredible," he said.

Pride 'about living in an inclusive community'

People of all ages turned out in support of Queensland's LGBTIQ community. ( ABC News: Andree Withey )

More than 20 politicians from all tiers and sides of government also joined the activities to highlight and celebrate the diversity of community.

Premier Annastacia Palaszczuk said it was fantastic to see so many families at the festival.

"This is about living in an inclusive community and I'm proud to be the leader of a state where we value and respect people," she said.

"We need to be a tolerant society but we also need to be respectful."

Deputy Premier Jackie Trad agreed.

"It's fantastic - it's a great indication of the level of community support and how big the LGTIQ community is in Queensland," she said.