HALLANDALE BEACH, Fla. — Just around the corner from the poker room and within shouting distance of the greyhound track here at the Mardi Gras Casino, the Amazing Spider-Man slot machine beckons gamblers with its spinning masked superhero and his nemesis the Green Goblin.

At the touch of a button, this Spider-Man can pay out fortunes or, more typically, deplete wallets.

But in the recently renewed battle over casino gambling in Florida this year, the popular Spider-Man slot machine delivers a different sort of jolt altogether. Spider-Man is one of a stable of Marvel superheroes that the Walt Disney Company acquired for $4 billion in 2009 and that continue to appear on slot machines, Internet slot machines and state lottery tickets. The lottery tickets have featured Iron Man and the Avengers.

Disney, a powerhouse in Florida because of its financial might and its sway over the tourism industry, has long led the fight against the expansion of casinos in the state, arguing successfully that gambling tarnishes Florida’s coveted family-friendly brand.

This year is no exception. For the second time in two years, state lawmakers are preparing to decide whether Las Vegas-style resort casinos should be allowed to open in Florida, a move that Disney hopes to thwart again. The company is so opposed to gambling that not even Disney cruise ships offer casinos, a mainstay of major cruise liners.