Background: Monoclonal antibody F77 raised against the PC3 cells recognizes human prostate cancers.

Results: Co-transfections with glycosyltransferase genes showed FUT1 and one of GCNT1, GCNT2 or GCNT3 is necessary for F77 antigen expression in mammalian cells.

Conclusion: F77 antigen is expressed on glycan structures composed of Fucα1→2Galβ1→4GlcNAcβ1→6Gal/GalNAc.

Significance: Defining F77 epitope provides a basis for clinical applications of this antibody.