Jo-Jo's restaurant, a Queen Street Mall landmark for 37 years, will close its doors next month, its flamboyant owner Stefan Ackerie announced on Wednesday.

Having originally opened as a small cafe in 1980 to service clients of Mr Ackerie's hair salon, Jo-Jo's grew rapidly to become one of the CBD's best-known and long-lasting eateries.

Jo-Jo's has been a mainstay of the Queen Street Mall for decades. Credit:Cameron Atfield

That is to end on March 5, when the rainbow-themed entrance will close for the final time.

But Mr Ackerie said the brand would live on at Stefan headquarters at South Brisbane.