The lineup for the first Democratic presidential debate is set.

After much speculation, NBC announced on Friday that former Vice President Joe Biden Joe BidenPelosi slams Trump executive order on pre-existing conditions: It 'isn't worth the paper it's signed on' Hillicon Valley: Subpoenas for Facebook, Google and Twitter on the cards | Wray rebuffs mail-in voting conspiracies | Reps. raise mass surveillance concerns Fox News poll: Biden ahead of Trump in Nevada, Pennsylvania and Ohio MORE will share the stage with Sen. Bernie Sanders Bernie SandersThe Hill's Campaign Report: Trump faces backlash after not committing to peaceful transition of power Bernie Sanders: 'This is an election between Donald Trump and democracy' The Hill's 12:30 Report: Trump stokes fears over November election outcome MORE (I-Vt.), South Bend, Ind., Mayor Pete Buttigieg Pete ButtigiegBillionaire who donated to Trump in 2016 donates to Biden The Hill's Morning Report - Sponsored by Facebook - GOP closes ranks to fill SCOTUS vacancy by November Buttigieg stands in as Pence for Harris's debate practice MORE and Sen. Kamala Harris Kamala HarrisHundreds of lawyers from nation's oldest African American sorority join effort to fight voter suppression Biden picks up endorsement from progressive climate group 350 Action 3 reasons why Biden is misreading the politics of court packing MORE (D-Calif.) on June 27.

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Meanwhile, Sen. Elizabeth Warren Elizabeth WarrenHillicon Valley: Subpoenas for Facebook, Google and Twitter on the cards | Wray rebuffs mail-in voting conspiracies | Reps. raise mass surveillance concerns On The Money: Anxious Democrats push for vote on COVID-19 aid | Pelosi, Mnuchin ready to restart talks | Weekly jobless claims increase | Senate treads close to shutdown deadline Democratic senators ask inspector general to investigate IRS use of location tracking service MORE (D-Mass.) will find herself separated from the other top-tier candidates when she takes the stage on June 26.

Here are five takeaways from the debate lineup:

1. Warren stands apart

Warren’s frequent policy rollouts and dogged campaigning has helped propel her to top-tier status in recent weeks, with several surveys showing her either statistically tied with or even overtaking Sanders in key early primary and caucus states.

But when she heads to Miami later this month for the first Democratic presidential debate, she’ll find herself as the only top-tier candidate on stage. The Massachusetts Democrat is slated to debate on June 26, while her chief rivals, Biden and Sanders, won’t appear until June 27.

That arrangement denies Warren a rare opportunity to directly challenge her fellow top-tier contenders, and consequently a chance to further boost her standing in the primary field.

But Warren’s separation from the rest of the top-tier contenders may not be a bad thing for her. That’s because she won’t have to compete with other top-tier candidates for valuable attention, while also having an opportunity to dominate the stage on June 26.

2. Biden and Sanders will have a chance to go at it

Few candidates are as different as Biden and Sanders. One has cast his campaign as a blunt effort to defeat President Trump Donald John TrumpSteele Dossier sub-source was subject of FBI counterintelligence probe Pelosi slams Trump executive order on pre-existing conditions: It 'isn't worth the paper it's signed on' Trump 'no longer angry' at Romney because of Supreme Court stance MORE and restore the political norms that preceded his administration, while the other has called for democratic socialism and political revolution.

On June 27, they’ll go head-to-head for the first time.

For Sanders, the debate presents an opportunity to broaden his base of support and potentially cut into Biden’s wide lead in the polls. Biden, on the other hand, will have a chance to challenge Sanders’s progressivism and make his case for a more centrist-minded approach to governing.

Sanders hasn’t directly attacked Biden yet. But he has delivered a few veiled swipes at the former vice president. During a speech at the California Democratic Party convention earlier this month, Sanders criticized the idea of a “middle ground” approach to politics, an unmistakable dig at Biden’s reputation as a moderate.

Biden took an implied shot at Sanders this week, saying at a fundraising event in Chicago that while the country is in need of change, “socialism” is not the answer.

3. The second night is stacked

With four of the primary contest’s top five contenders taking the stage on June 27, it’s clear that the second night of the debate carries extra weight.

Biden, Sanders, Buttigieg and Harris will share the stage that night, while a fifth top-tier candidate, Warren, will find herself separated from the pack on June 26.

That lineup raises the question of whether the second night may garner more attention than the first. But it also puts pressure on the leading candidates to stand out.

Unlike Warren, who has the opportunity to command the stage the first night, the four other top-tier candidates will have to jockey for attention. Still, that leaves each of them with a valuable opportunity to show off their debate chops.

4. Lower tier candidates have a couple paths

For candidates who have so far struggled to break out, the debate may be the opportunity they need to turn their fortunes around.

Best-selling author Marianne Williamson Marianne WilliamsonMarianne Williamson discusses speaking at People's Party Convention Fewer people watched opening night of Democratic convention compared to 2016 Marianne Williamson: Democratic convention 'like binge watching a Marriott commercial' MORE and tech entrepreneur Andrew Yang Andrew YangDoctor who allegedly assaulted Evelyn Yang arrested on federal charges The Hill's Campaign Report: Biden weighs in on police shootings | Who's moderating the debates | Trump trails in post-convention polls Buttigieg launches his own podcast MORE, the clearest political outsiders in the race, will both share the stage with Biden, Sanders, Buttigieg and Harris on June 27, as will other long shot candidates like former Rep. John Delaney John DelaneyCoronavirus Report: The Hill's Steve Clemons interviews Rep. Rodney Davis Eurasia Group founder Ian Bremmer says Trump right on China but wrong on WHO; CDC issues new guidance for large gatherings The Hill's Coronavirus Report: Kansas City Mayor Quinton Lucas says country needs to rethink what 'policing' means; US cases surpass 2 million with no end to pandemic in sight MORE (D-Md.), who announced his presidential bid nearly two years ago.

A standout performance in the presence of four top-tier candidates could give the lower-tier hopefuls a much-needed boost. But those second-night slots also carry the risk of lesser-known contenders being drowned out by more established candidates.

With Warren being the only breakout candidate on stage on June 26, her opponents may have a better chance to shine. Beyond Warren, however, that stage will still include high-profile Democrats like Sen. Cory Booker Cory Anthony Booker3 reasons why Biden is misreading the politics of court packing Bipartisan praise pours in after Ginsburg's death DHS opens probe into allegations at Georgia ICE facility MORE (D-N.J.) and former Rep. Beto O’Rourke (D-Texas), both of whom have proven adept at commanding crowds.

5. NBC, DNC will face a highwire act

Five hosts will moderate the debates, and they’re sure to face close scrutiny over everything from the questions they ask to their handling of candidate responses.

And with so many candidates on the stage at once, the moderators will be under intense pressure to stick to rigid time limits while ensuring that each contender gets a fair shake.

Democratic National Committee (DNC) Chairman Tom Perez Thomas Edward PerezClinton’s top five vice presidential picks Government social programs: Triumph of hope over evidence Labor’s 'wasteful spending and mismanagement” at Workers’ Comp MORE acknowledged the time constraints on Friday, conceding in an interview with MSNBC that the first debate may not offer candidates the chance to do the kind of “deep dives” on policy issues that they may be hoping for.

At the same time, the DNC itself will also find itself on the hook for any successes or failures in the first debate. After facing criticism for its handling of primary debates in 2016, the committee sought to open up the process, setting easy-to-meet requirements for candidates to appear on the debate stage.

Some candidates, however, have already raised concern about the debate.

Biden said this week that the crowded stages mean that candidates won’t be able to address questions with “any real depth.”

And Montana Gov. Steve Bullock Steve BullockMcConnell locks down key GOP votes in Supreme Court fight Senate Democrats demand White House fire controversial head of public lands agency Pence seeks to boost Daines in critical Montana Senate race MORE, who was among the four candidates who failed to make the debate stage, has complained that the DNC’s decision to omit one of his polls from its qualifying criteria unfairly shut him out of the event.