@biostudent84 : Thank you for your reply.

This project is part of my PhD work, which I have just started a few weeks ago in Stuttgart (Germany). I asked that question to nearly all people I know, but nobody seems to know why protoplast lysis works better with EDTA than without. Seems that some metals, if not complexed by EDTA, have some effect on the lysis procedure. But I cannot imagine which exact mechanism is responsible for that, to avoid this problem…

So, still the same question: if anybody can tell me, I would be glad!!

I also checked lots and lots of publications. Either I am to stupid to find this 😳 or nobody published something about it, or it is so simple that everybody thinks it is not worth mentioning… ❓