Chiellini had reportedly been struggling with a calf problem on Wednesday, but it was not initially thought to be serious.

But on Thursday Juventus confirmed that a scan had revealed a first grade tear to the player's soleus muscle in his left leg, which rules him out of contention for the match.

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The 30-year-old centre-back has been one of Juventus's key players this season, with some suggesting he is the key to stopping Barcelona's mighty strike force of Leo Messi, Neymar and Luis Suarez..

Juventus were already a long shot before Chiellini's injury, with bookmakers pricing them at 5-1 to win the match in 90 minutes and 12-5 to lift the trophy, against Barcelona at 13-20 and 4-11 respectively.

But without the Italian international, Juventus's hopes of winning the tournament - and with it, a historic treble - will lengthen further.

Juventus squad to face Barcelona: Buffon, Ogbonna, Pogba, Pepe, Marchisio, Morata, Tevez, Coman, Llorente, Barzagli, De Ceglie, Bonucci, Padoin, Pirlo, Vidal, Lichtsteiner, Sturaro, Storari, Matri, Evra, Rubinho, Pereyra.

WHO WILL REPLACE HIM?

Chiellini's place in the starting line-up will undoubtedly be taken by Andrea Barzagli, who has been in and out of the side since returning from a serious ankle injury just a few months ago.

Since his return the 34-year-old Italy international has played a key role, either at the heart of a back-four or on the right of a back three, producing solid defensive performances in key matches including the Serie A-clinching victory away at Sampdoria at the beginning of May.

OUR VIEW

As our article on Wednesday argued, Chiellini's ability to start an attacking move with a well-played pass from the centre of defence will be missed. But more important than that will be the psychological effect of heading to Berlin without the same back four that famously withstood Real Madrid's semi-final second-leg onslaught at the Bernabeu, where the likes of Cristiano Ronaldo and Gareth Bale were rendered completely ineffective. Feeding off the positive energy from that giant-slaying was Juve's best hope against a side in devastating form - now, it will be that much harder for them to do so.

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