DOG CLONING AT SOOAM

Dogs have been domesticated for thousands of years. They have been our protectors and friends; however, an average lifespan of a dog is about 10~15 years, much shorter than that of a human being. Sooam Biotech Research Foundation is able to prolong the companionship with your dog by bringing back the memories that you have with your friend. Cloning technology is possible at Sooam for any dog no matter its age, size, and breed. Sooam not only performs dog cloning research, but we also heal the broken hearts.

When your dog has passed away, DO NOT place the cadaver inside the freezer.

Then, patiently follow these steps: 1. Wrap the entire body with wet bathing towels.

2. Place it in the fridge(not the freezer) to keep it cool.

* Please take into account that you have approximately 5 days to successfully extract and secure live cells.

* Please contact our specialists by filling out the service application form at www.notyoubutyou.com

Email : notyoubutyou@adbrf.org

Telephone : +82 70 7722 9354

- Biopsy Instructions For Veterinarians -

Each case is unique, please consult one of our specialists before consulting your veterinarian

1. Pet Preparation

Use appropriate anesthesia and/or sedation as required. Apply general anesthesia if the pet's health permits. In case of local anesthesia, please obtain the biopsy sample from the farthest possible site from the site of anesthesia application.

2. Aseptic Preparation of Biopsy Site

The biopsy area must be shaved and cleansed with a series of betadine, chlorhexidine and alcohol scrubs. THIS IS A VERY IMPORTANT STEP as contamination of the biopsy sample may lead to undesirable results. Hair can be a source of contamination in samples. The biopsy area should be covered with a sterile drape if applicable. Thoroughly sterilize all instruments necessary for the biopsy procedure.

3. Biopsy Procedure Guidelines

You will need 3 to 4 refrigerated vials for pre-mortem biopsy (6 to 8 for post-mortem biopsy). Remove vials from the refrigerator no more than half hour before surgery. You will also need one bag of Parafilm Sealing Tape strips for use after the samples are placed into the vials.

Biopsy samples should be obtained from the ventral abdomen in standard aseptic fashion If the dog is alive, 2 biopsy samples (about the size of an American Nickel coin) per vial should be prepared. Biopsy of the skin (epidermal tissue) is preferred. If the dog has passed away, 3-4 biopsy samples per vial should be prepared. Biopsies of the skin (epidermal tissue) as well as muscle tissue (in separate vials) are preferred. To clarify, in the case of a deceased companion (post-mortem), we recommend collection of 6-8 vials total: 3-4 vials containing epidermal tissues and 3-4 vials containing muscle tissues. Each vial contains 3-4 biopsy samples, or 'punches.' Please keep the body of the companion refrigerated (not freezing) until confirmation as additional biopsies may be necessary.

Store each biopsy sample in a separate sterile vial containing sterile saline (or PBS) containing 1% antibiotic solution (Pen-Strep or Gentamicin but not Baytril). Blood tubes without anticoagulant as well as 50 mL tubes and specimen cups can be used.

Store vials in the refrigerator but DO NOT FREEZE.

The biopsy site should be closed using appropriate suture materials.

4. Tools and Technique

Use a 8 mm biopsy punch tool for all samples. Please rinse the tool in alcohol between each biopsy.

The instrument is rotated on the surface of the skin until it cuts through all the layers, including the epidermis, dermis, and the most superficial parts of the subcutis. If biopsy remains in the tool, remove with sterile forceps. If biopsy fails to separate from the subcutis, remove with sterile forceps and scalpel.

If a 8 mm punch tool is not available, biopsy can also be performed using surgical scissors.

Appropriate suture material should be used to close each biopsy site.

5. Biopsy Handling