A row has broken out over a new Liberal National Party advertisement highlighting union links to bikie gangs, as claims emerge that outlaw motorcycle clubs discussed donating to the LNP.

Queensland Premier Campbell Newman said criminal motorcycle gangs were backing Labor because Labor had opposed the state's Vicious Lawless Association Disestablishment (VLAD) laws.

At the leaders' debate on Friday night, Mr Newman suggested "dirty" money from criminal gangs could be financing Labor's election campaign though its union donors, specifically naming the Construction, Forestry, Mining and Energy Union (CFMEU).

The union has rubbished the Premier's statement as "ridiculous and untrue".

The LNP's new 40-second advertisement, featuring Peter Simpson from the Electrical Trade Union (ETU) addressing a rally outside State Parliament, was posted on the LNP's Facebook and YouTube accounts on Sunday morning.

The advertisement claimed unions were proud associates of bikie gangs, who brought "wads of cash" to support them.

"Some people are a bit nervous about being associated with bikies, I'm proud to say the Electrical Trade Union aren't," Mr Simpson said in the video, which was originally posted online in full in 2013.

"So stand tall, do more of this s**t and let's get out on the streets and show them."

ETU communications officer Andrew Irvine released a written statement saying Mr Newman's allegations were "getting more bizarre and more desperate".

"If Mr Newman has any evidence, he should make a police complaint immediately," he said.

"The ETU's independently audited accounts are registered with the Fair Work Commission and all of our political donations are disclosed to the ECQ.

"Obviously, Newman's smear against the CFMEU is baseless and he is now searching around for a new target for his smear."

The video also stated the ETU gave $206,374.50 to Queensland Labor, which Mr Irvine disputed.

"It should also be noted that the $200,000 donation to the ALP is a national figure not a state one," the statement said.

In a written statement, a CFMEU spokeswoman said Mr Newman's claims were "ridiculous and untrue".

"The CFMEU's account and donations are independently audited and disclosed on a regular basis," the spokeswoman said.

"No allegations of this nature have been made in Queensland."

Queensland Labor's campaign director Anthony Chisholm said Mr Newman needed to back up his claims immediately.

"Queenslanders are seeing an increasingly desperate Premier," Mr Chisholm said.

"If Mr Newman can't back it up today then I am calling on Mr Newman to withdraw the TV ad they are running which makes this claim."

Public raising issue of bikie links to Labor: Newman

On Saturday afternoon, Mr Newman suggested people "google it" to find evidence of bikie links.

Sorry, this video has expired Campbell Newman stands by LNP allegations of union, bikie links

At a press conference on Sunday, Mr Newman said he was basing his claims on conversations with the general public.

"As I go around the state, people say to me 'Campbell, we know that the unions and criminal gangs have a close association'," Mr Newman said on Sunday.

"They've seen that and they know that and they also know that bikies are backing the Labor Party and they're saying to me, 'be strong'."

Opposition Leader Annastacia Palaszczuk has hit back, saying if there was any evidence, Mr Newman should refer it to the police.

"This is a Premier who said he would not get into the gutter. Well, today this is an all-time low," she said.

"He just wants to resort to whatever smear campaign he can possibly come up with in the week before an election."

She said it was up to individual unions to decide if patched members should be banned from their organisation.

Bikies 'discussed donating to LNP campaign'

The row came as a former member of an outlaw motorcycle club said he would not be surprised if the LNP had received modest campaign donations from bikie members, and another man claiming to be a bikie had his donation to the LNP refunded.

Sorry, this video has expired Annastacia Palaszczuk slams Labor, bikie gang link allegation

Russell Wattie, who is standing as an independent candidate in Maryborough, said the LNP should check its books before accusing the Labor Party of accepting donations from bikies.

Mr Wattie said the issue of donating to the LNP was discussed by patched bikie clubs and members several weeks ago.

"They should definitely take a look at their own backyard," Mr Wattie said.

"I know for a fact that it was suggested three weeks ago by a meeting of clubs to support the LNP, with the statement being, better the devil you know than the one you don't.

"To point the finger at the Labor Party and to say they have got funds coming towards them — he should probably check his own party donors first and see where the money has come from."

Meanwhile, Angelo Garozzo shared a post on Facebook with an image of what appeared to be a thank-you message from the LNP for a donation.

"I just donated money to Campbell Newman, so he just accepted money from a bikie," Mr Garozzo wrote.

LNP state director Brad Henderson, however, said the money was sent back.

"The party is unaware of any donation from bikies apart from one who admitted it was a deliberate stunt to distract from the established links between bikies and some unions," Mr Henderson said.

"The LNP's tough stance against criminal motorcycle gangs to make Queensland safer is well known.

"That donation has been refunded."

Mr Henderson said the stunt was further evidence that bikies want "a weak Labor government in power to repeal the tough LNP laws cracking down on criminal gangs".

In a follow-up post on his Facebook page, Mr Garozzo said "of course it's a bloody stunt".

"Just like new man's [sic] stunt accusing Labor of accepting bikie money."