Alkyl dimethyl benzyl ammonium chloride (ADBAC) and didecyl dimethyl ammonium chloride (DDAC) are common ingredients in household bathroom and kitchen cleaning sprays. ADBAC + DDAC cause reproductive toxicity in mice. The aim of the present study was to investigate gender-specific reproductive effects from ADBAC + DDAC. Female reproduction was assessed through ovulation, oocyte implantation, and estrus cycling. Male reproductive function was assessed by sperm concentration, motility, and viability. Numbers of corpora lutea were not different after 2 weeks, but decreased after 8 weeks of ADBAC + DDAC exposure. Dams exposed for 5 weeks to ADBAC + DDAC spent significantly less time in estrus. ADBAC + DDAC exposed males exhibited declines in both sperm concentration and motility, but not sperm viability. Subfertility in mice from ADBAC + DDAC exposure is, therefore, mediated through reproductive disturbances in both females and males. While the effect of ADBAC + DDAC exposure on human health is unclear, widespread exposure necessitates further consideration of their potential reproductive toxicity.