Men whose female partners had unprotected sex with a HIV-positive circus acrobat have come forward following a nationwide appeal.

Two more possible victims of Godfrey Zaburoni, who allegedly had sex with multiple women while knowing he was carrying the virus, called the Queensland Health Hotline today.

HIV positive ... Godfrey Zaburoni

The pair raised their concerns, while a further 18 people called the hotline yesterday.

Queensland Health today confirmed men who had relations with women Mr Zaburoni had unprotected sex with had come forward for testing.