House Speaker Paul Ryan told his Republican colleagues in October that he was cutting ties with Donald Trump, saying he would defend the presidential candidate “not now, not in the future,” according to an audiotape of the call released on Monday by Breitbart.

“There are basically two things that I want to make really clear, as for myself as your speaker. I am not going to defend Donald Trump — not now, not in the future,” Ryan said on the Oct. 10 conference call to Republican members, according to the tape. “As you probably heard, I disinvited him from my first congressional district GOP event this weekend — a thing I do every year. And I’m not going to be campaigning with him over the next 30 days.”

Ryan’s harsh comments came just three days after footage from Trump’s 2005 appearance on “Access Hollywood” revealed him making vulgar comments about sexually assaulting women.

The thrust of Ryan’s comments was widely reported at the time, but the audio marks the first time his precise words were made public.

Then-candidate Trump lashed out at Ryan on Twitter for the remarks.

“Paul Ryan should spend more time on balancing the budget, jobs and illegal immigration and not waste his time on fighting Republican nominee,” Trump wrote in October.

It’s unclear why the tape of Ryan, who is now helping Trump shepherd the repeal and replacement of ObamaCare through the House, was released Monday by Breitbart, which used to be run by top White House adviser Steve Bannon.

But more-conservative Republicans, especially those in the Tea Party-influenced Freedom Caucus, have trashed the GOP health insurance bill, calling it “ObamaCare 2.0” and “ObamaCare Lite.”

They believe President Obama’s health care plan should be repealed outright.

Breitbart may have provided insight on the timing of the tape’s release in commentary in the story.

“This is the first major initiative that Trump has worked on with Ryan — and the fact it is going so poorly calls into question whether Speaker Ryan, the GOP’s failed 2012 vice presidential nominee who barely supported Trump at all in 2016, really understands how Trump won and how to win in general,” Breitbart wrote.

Ryan spokesperson Brendan Buck said the revelations are nothing new. “The world is aware of this history,” he told the conservative website. “And obviously a lot has happened since then. As everyone knows.”