The Upright Citizens Brigade Theatre will be moving out of its theater located in Manhattan’s Chelsea neighborhood, home to its brand of improvisational comedy for more than a decade, and relocating uptown to Hell’s Kitchen.

Upright Citizens Brigade, the comedy group co-founded by Amy Poehler, has acquired a lease for the former performance space of the Pearl Theatre Co., which filed for bankruptcy in June. The new theater is located at 555 W. 42nd St. The move is scheduled to occur by the end of November and will be commemorated with special performances to celebrate the change and bid farewell to the Chelsea venue, which the improv theater took over in 2003.

Located on West 26th Street in a basement below a Gristedes supermarket, the Chelsea theater has become hallowed ground in New York City’s comedy scene and served as a launchpad for the careers of numerous actors and writers. Live versions of the Comedy Central television shows “Broad City” and what became “The President Show” were performed in the Chelsea theater.

Upright Citizens Brigade had long been searching for a venue that complies with the Americans with Disabilities Act, said Shannon O’Neill, artistic director of Upright Citizens Brigade Theatre New York. The theater plans to begin performing at the new Hell’s Kitchen location at the beginning of December, she said.

“We have a very diverse group of students, performers and patrons and we want to be able to serve all of them,” Ms. O’Neill said. “Right now, the Chelsea theater doesn’t allow that.”