Nanomaterials can enable plants with novel and augmented functions. This Arabidopsis plant with carbon nanotubes inside its leaves has augmented light energy capture and could act as a photonic biochemical detector. Credit: Juan Pablo Giraldo

Plants have many valuable functions: They provide food and fuel, release the oxygen that we breathe, and add beauty to our surroundings. Now, a team of MIT researchers wants to make plants even more useful by augmenting them with nanomaterials that could enhance their energy production and give them completely new functions, such as monitoring environmental pollutants.

In a new Nature Materials paper, the researchers report boosting plants' ability to capture light energy by 30 percent by embedding carbon nanotubes in the chloroplast, the plant organelle where photosynthesis takes place. Using another type of carbon nanotube, they also modified plants to detect the gas nitric oxide.

Together, these represent the first steps in launching a scientific field the researchers have dubbed "plant nanobionics."

"Plants are very attractive as a technology platform," says Michael Strano, the Carbon P. Dubbs Professor of Chemical Engineering and leader of the MIT research team. "They repair themselves, they're environmentally stable outside, they survive in harsh environments, and they provide their own power source and water distribution."

Strano and the paper's lead author, postdoc and plant biologist Juan Pablo Giraldo, envision turning plants into self-powered, photonic devices such as detectors for explosives or chemical weapons. The researchers are also working on incorporating electronic devices into plants. "The potential is really endless," Strano says.

Supercharged photosynthesis

The idea for nanobionic plants grew out of a project in Strano's lab to build self-repairing solar cells modeled on plant cells. As a next step, the researchers wanted to try enhancing the photosynthetic function of chloroplasts isolated from plants, for possible use in solar cells.

Chloroplasts host all of the machinery needed for photosynthesis, which occurs in two stages. During the first stage, pigments such as chlorophyll absorb light, which excites electrons that flow through the thylakoid membranes of the chloroplast. The plant captures this electrical energy and uses it to power the second stage of photosynthesis—building sugars.

Near infrared fluorescence of carbon nanotubes (orange) infiltrated inside leaves (green) could boost photosynthesis and enable the detection of biochemicals and pollutants. Credit: Juan Pablo Giraldo and Nicole M. Iverson

Chloroplasts can still perform these reactions when removed from plants, but after a few hours, they start to break down because light and oxygen damage the photosynthetic proteins. Usually plants can completely repair this kind of damage, but extracted chloroplasts can't do it on their own.

To prolong the chloroplasts' productivity, the researchers embedded them with cerium oxide nanoparticles, also known as nanoceria. These particles are very strong antioxidants that scavenge oxygen radicals and other highly reactive molecules produced by light and oxygen, protecting the chloroplasts from damage.

The researchers delivered nanoceria into the chloroplasts using a new technique they developed called lipid exchange envelope penetration, or LEEP. Wrapping the particles in polyacrylic acid, a highly charged molecule, allows the particles to penetrate the fatty, hydrophobic membranes that surrounds chloroplasts. In these chloroplasts, levels of damaging molecules dropped dramatically.

Using the same delivery technique, the researchers also embedded semiconducting carbon nanotubes, coated in negatively charged DNA, into the chloroplasts. Plants typically make use of only about 10 percent of the sunlight available to them, but carbon nanotubes could act as artificial antennae that allow chloroplasts to capture wavelengths of light not in their normal range, such as ultraviolet, green, and near-infrared.

With carbon nanotubes appearing to act as a "prosthetic photoabsorber," photosynthetic activity—measured by the rate of electron flow through the thylakoid membranes—was 49 percent greater than that in isolated chloroplasts without embedded nanotubes. When nanoceria and carbon nanotubes were delivered together, the chloroplasts remained active for a few extra hours.

The researchers then turned to living plants and used a technique called vascular infusion to deliver nanoparticles into Arabidopsis thaliana, a small flowering plant. Using this method, the researchers applied a solution of nanoparticles to the underside of the leaf, where it penetrated tiny pores known as stomata, which normally allow carbon dioxide to flow in and oxygen to flow out. In these plants, the nanotubes moved into the chloroplast and boosted photosynthetic electron flow by about 30 percent.

Yet to be discovered is how that extra electron flow influences the plants' sugar production. "This is a question that we are still trying to answer in the lab: What is the impact of nanoparticles on the production of chemical fuels like glucose?" Giraldo says.

Imaging the fluorescence of carbon nanotubes inside leaves of an Arabidopsis plant using a single particle near infrared microscope. Credit: Bryce Vickmark

Lean green machines

The researchers also showed that they could turn Arabidopsis thaliana plants into chemical sensors by delivering carbon nanotubes that detect the gas nitric oxide, an environmental pollutant produced by combustion.

Strano's lab has previously developed carbon nanotube sensors for many different chemicals, including hydrogen peroxide, the explosive TNT, and the nerve gas sarin. When the target molecule binds to a polymer wrapped around the nanotube, it alters the tube's fluorescence.

"We could someday use these carbon nanotubes to make sensors that detect in real time, at the single-particle level, free radicals or signaling molecules that are at very low-concentration and difficult to detect," Giraldo says.

By adapting the sensors to different targets, the researchers hope to develop plants that could be used to monitor environmental pollution, pesticides, fungal infections, or exposure to bacterial toxins. They are also working on incorporating electronic nanomaterials, such as graphene, into plants.

"Right now, almost no one is working in this emerging field," Giraldo says. "It's an opportunity for people from plant biology and the chemical engineering nanotechnology community to work together in an area that has a large potential."

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