a, Gating strategy for CD24+ cancer cells and Siglec-10+ TAMs in primary human tumours; after debris and doublet removal, cancer cells were assessed as DAPI−CD14−EpCAM+ and TAMs were assessed as DAPI−EpCAM−CD14+CD11b+. Plots are representative of six experimental replicates. b, Left, representative flow-cytometry histogram measuring the expression of Siglec-10 (blue shaded curves) versus isotype control (black lines) by non-cancerous peritoneal macrophages; numbers above bracketed line indicate the percentage of macrophages positive for expression of Siglec-10. Right, frequency of peritoneal macrophages positive for Siglec-10 among all peritoneal macrophages as defined by isotype controls (n = 9 donors). c, Gating strategy for CD24+ cells and Siglec-10+ cells among PBMC cell types; after debris and doublet removal, monocytes were assessed as DAPI−CD3−CD14+; T cells were assessed as DAPI−CD14−CD3+; natural killer (NK) cells were assessed as DAPI−CD14−CD3−CD56+; B cells were assessed as DAPI−CD56−CD14−CD3−CD19+. Plots are representative of two experimental replicates. d, Frequency of PBMC cell types positive for Siglec-10 (blue shaded bars) or CD24 (red shaded bars) out of total cell type (n = 3 donors). e, Left, flow-cytometry-based measurement of the surface expression of Siglec-10 on primary human donor-derived macrophages either unstimulated (top) or after stimulation with M2-polarizing cytokines TGFβ1 and IL-10 (bottom), numbers above bracketed line indicate the per cent of CD11b+ macrophages positive for expression of Siglec-10. Right, frequency of primary human donor-derived macrophages positive for Siglec-10 either without stimulation (unstimulated, M0) or following stimulation with TGFβ1 and IL-10 (stimulated, M2-like) (n = 30 unstimulated donors, 33 stimulated donors; unpaired, two-tailed Student’s t-test, ****P < 0.0001, data are mean ± s.e.m.). f, Flow-cytometry-based measurement of phagocytosis of MCF-7 cells by unstimulated donor-derived macrophages (white data points) versus TGFβ1 and IL-10-stimulated donor-derived macrophages (n = 3 donors, unpaired, one-tailed t-test, *P = 0.0168). g, Left, flow-cytometry-based measurement of the surface expression of Siglec-10 on matched, primary donor-derived macrophages either unstimulated (grey shaded curve), or after stimulation with TGFβ1 and IL-10 (blue line), or IL-4 (green line). Right, frequency of matched, human donor-derived macrophages positive for Siglec-10 either without stimulation (unstimulated, M0), or after stimulation with TGFβ1 and IL-10 (blue dots), or stimulated with IL-4 (n = 4 donors). Data are mean ± s.e.m. Source data