Jonathan Groff has never been in a production of “Little Shop of Horrors.” Unless, that is, you count the hours in the kitchen of his childhood home, belting out “Skid Row.”

Now the two-time Tony nominee, a star of the television shows “Mindhunter” and “Looking,” is going to lead a revival of the musical in a 270-seat Off Broadway theater in Hell’s Kitchen.

“It’s a scary, funny, genius show, and I’m excited to celebrate it,” Mr. Groff said in an interview. “And to do it in that small space — it seems like an illegal amount of fun.”

The production, which is scheduled to begin previews Sept. 17 and to open Oct. 17 at the Westside Theater, will reunite Mr. Groff with the director Michael Mayer; they previously collaborated on “Spring Awakening,” the show that brought the actor his first Tony nomination. Mr. Groff returned to Broadway as King George in “Hamilton,” and that role brought him his second nomination. His other familiar credits: He voices Kristoff in the “Frozen” films, and he had a recurring role on “Glee.”