WASHINGTON — Pakistan’s top military spy agency has arrested some of the Pakistani informants who fed information to the Central Intelligence Agency in the months leading up to the raid that led to the death of Osama bin Laden, according to American officials.

Pakistan’s detention of five C.I.A. informants, including a Pakistani Army major who officials said copied the license plates of cars visiting Bin Laden’s compound in Abbottabad, Pakistan, in the weeks before the raid, is the latest evidence of the fractured relationship between the United States and Pakistan. It comes at a time when the Obama administration is seeking Pakistan’s support in brokering an endgame in the war in neighboring Afghanistan.

At a closed briefing last week, members of the Senate Intelligence Committee asked Michael J. Morell, the deputy C.I.A. director, to rate Pakistan’s cooperation with the United States on counterterrorism operations, on a scale of 1 to 10.

“Three,” Mr. Morell replied, according to officials familiar with the exchange.

The fate of the C.I.A. informants arrested in Pakistan is unclear, but American officials said that the C.I.A. director, Leon E. Panetta, raised the issue when he travelled to Islamabad last week to meet with Pakistani military and intelligence officers.