At least 10 panthers and bobcats in Florida are having (or had) trouble walking, and wildlife experts don’t know why. Two cats died recently from other causes, but scientists confirmed they, too, were suffering from whatever is going around. The Florida Fish and Wildlife Conservation Commission (FWC) is monitoring the status of the cats on trail cameras.

The list of possible panther and bobcat afflictions is long. Researchers are testing for infectious diseases, nutritional deficiencies, exposure to heavy metals and multiple toxins including rat poison and toxic algae.

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The FWC always completes necropsies — autopsies for animals — even if they already know the cause of the panther’s death, said Mark Cunningham, a veterinarian at the commission. On Wednesday, researchers examined the two dead cats and tested for toxins and contaminants. The bobcat was injured during a fight and subsequently was hit by a vehicle. The panther was euthanized after she was injured by a vehicle and contracted an infection, Cunningham said.

The scientists took tissue samples from the deceased panther and bobcat, to better understand what might have caused the damage in their nervous systems.

“Our veterinarian staff have submitted samples to specialized laboratories,” said Michelle Kerr, a spokeswoman at the FWC’s Fish and Wildlife Research Institute. Neither of the animals tested positive for feline leukemia, Kerr said, a disease that has killed the endangered species previously.

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“Affected animals tested negative for multiple infectious diseases, but we wouldn’t say infectious diseases are ruled out completely,” Kerr said.

In addition to the deceased cats, trail camera footage revealed eight other panthers, most of them kittens, and an adult bobcat showing similar symptoms. So far, the affected cats have been observed in Collier, Lee and Sarasota counties. At least one potentially affected panther was photographed in Charlotte County.

Florida panthers are an endangered species. They’ve slowly come back after development and habitat destruction cut their numbers down to 20 wild panthers at the end of the 20th century. Today, FWC estimates that there are between 120 and 230 adult panthers living in southern Florida.

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Samantha Wisely, a professor of wildlife ecology at the University of Florida, who is not involved with the research on the affected bobcats and panthers, said big cats can pick up infectious diseases in a variety of ways.

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Panthers can get diseases from preying on other animals that might have a disease, or they can drink contaminated water. But since the causes of the disease could be numerous, Wisely said it’s important to look at multiple possibilities.

“When you don’t have a good sense of what it is, you really want to cast your net widely,” she said.

The scientists at FWC are also looking at whether a common rat poison, bromethalin, might have played a role in the neurological damage. The Environmental Protection Agency advises that individuals should always remove the dead rodents right away after they are killed by rodenticides.

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Another cause of the cat’s condition might be toxic algae, according to the FWC. Earlier this summer, toxic algae killed three dogs after they played in a North Carolina pond. The toxins released by the algae have recently led to the deaths of multiple marine animals in Florida and elsewhere along the Gulf Coast.

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“Numerous diseases and possible causes have been ruled out; a definitive cause has not yet been determined,” Gil McRae, director of the Fish and Wildlife Research Institute, said in a statement. “We’re working with the U.S. Fish and Wildlife Service and a wide array of experts from around the world to determine what is causing this condition.”

Researchers are calling on the public to help identify more panthers that might be potentially showing signs of the condition. The FWC is asking people to upload trail camera videos that show any cat activity, and to call and report any that are seen injured or dead.