(CNN) The dead submarine crew hadn't moved from their stations for nearly 150 years when the vessel was raised from the ocean in 2000. Whatever killed them happened so suddenly that they never made a run for the escape hatch. What's more, they had no obvious physical injuries.

There was no major damage to the hull that could be definitively traced back to the day the H.L. Hunley, a 40-foot-long Confederate submarine, sank to the ocean floor off Charleston, South Carolina, on February 17, 1864.

Researchers had unsealed the crew compartment of the submarine, but they have yet to find conclusive evidence of how the eight men aboard died.

But in research published Wednesday in the journal Plos One one group of scientists thinks they've finally cracked the case of what killed the crew so swiftly.

Shrinking down, blowing up

The Hunley became the first sub to sink an enemy ship in battle: the USS Housatonic. But sometime after, it went down, too.

It sank the enemy ship with a 135-pound torpedo, which was filled with black powder and attached to a pole 16 feet from the ship's hull. The study authors say the torpedo is the key -- but many have wondered how an explosion could've killed the entire crew without leaving a trace.

Rachel Lance set off explosions near a miniature replica of a Civil War submarine.

To answer this question, biomechanist Rachel Lance designed a model of the Hunley, one-sixth the length of the 40-foot-long submarine. The model, built by Durham-based sculptor Tripp Jarvis, was christened the CSS Tiny.

Lance, then a graduate student at Duke University and an engineer with the Naval Surface Warfare Center , decided she would set off test explosions next to the model submarine. So she found an eight-acre pond on a family-run farm in St. Louis, North Carolina. Bert Pitt of Pitt Family Farms agreed to let Lance use the pond to conduct her experiments.

"Initially, when she was talking about blasting, I was a little concerned," said Pitt, 65, a sixth-generation family farmer, whose grandchildren now make eight generations.

Pitt recalled the wires snaking into the lake and the charges that detonated beneath the surface, splashing water into the air like a large firecracker, he said. One of his grandkids got to press the button.

"It had a little geyser to it," he said. "It was neat to see."

Pitt, a self-proclaimed history buff, had always been interested in the Civil War. He has ancestors who were in the North Carolina Regiments, and at least one of them is buried in their own family graveyard. The house he lives in was built in 1830, before the Hunley sank.

He keenly eyed reports about the Hunley on the History Channel and the National Geographic Channel.

"They were sitting perfectly still in that submarine," Pitt said. "I think people would like to know what did happen to the crew. Everything about the story is intriguing."

Without a trace

Suspended inside the CSS Tiny was a small pressure gauge, which revealed how the sub's own torpedo blast could have killed the Hunley crew without leaving a lasting mark: the shock wave created by the blast.

The shock wave hit the Hunley's hull, which was less than an inch thick, said Lance, lead author of the new study. The metal bent ever so slightly but fast enough to transfer the blast wave to the inside of the cabin.

That wave then traveled through the cabin, hitting each of the eight crewmembers, traveling through their bodies. But the real damage, Lance said, probably occurred when the pressure wave reached their lungs.

"The issue is when it's passing through (the tissues) and it suddenly hits air," she said.

Shock waves, like sound waves, travel quickly in water and solids but not air. The wave slows as it hits the lung, Lance said, and "that energy has to transmit somewhere."

The end result: The blood vessels in the lungs can rupture, known as a pulmonary hemorrhage.

"It was ... noted that men could be killed or disabled at considerable distance" from an explosive, Dr. Thomas Chiffelle, a pathologist from Albuquerque, New Mexico, wrote in a 1966 report for the US Department of Defense. "The man or animal may be killed outright, without external signs of injury, but often with blood-tinged froth or frank blood appearing in the nose and mouth."

It is possible to survive a blast wave from far enough, according to Chiffelle's accounts. Witness accounts from the night of the Hunley's sinking claimed that there was a blue light coming from the ocean. Some speculated that it was the Hunley crew signaling that they'd accomplished their mission.

Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Since 2000, scientists, historians and a genealogist have studied the first submarine to sink an enemy vessel. The H.L. Hunley did just that more than 150 years ago, on February 17, 1864, during the American Civil War. Hide Caption 1 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – The 40-foot submarine was brought up amid much fanfare off Charleston, South Carolina, in August 2000. Author Clive Cussler and a team discovered the Hunley five years earlier, buried in the sand more than 100 yards beyond its target, the USS Housatonic. Hide Caption 2 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Conservators later began a new process -- filling the tank that holds the Hunley with chemicals that helped strip away what is called "concretion": organic material that has coated the hull and interior. Hide Caption 3 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Visitors to a laboratory and exhibit hall in North Charleston, South Carolina, can gaze down on the Hunley on weekends. The large tank is empty when scientists in the Hunley Project are at work. Hide Caption 4 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – The hull is in pretty good shape, despite exposure to sea currents and elements for decades. The eight-member Confederate crew, sitting on the port side, turned a propeller by hand. Hide Caption 5 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Several years ago, then-senior archaeologist Maria Jacobsen found a gold coin that belonged to submarine commander Lt. George Dixon. Hide Caption 6 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Lt. George Dixon, according to legend, received this coin as a good-luck charm from his beloved, said to be from Mobile, Alabama. It was bent by a bullet when he was wounded at the Battle of Shiloh, two years before the Hunley made history. He had it engraved and carried it the rest of his life. It was found with his remains by Hunley Project scientists. Hide Caption 7 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Paul Mardikian, left, senior conservator, and Philippe de Vivies remove material from a piece of the submarine. Hide Caption 8 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – One of the personal belongings found inside the Hunley, a watch belonging to Lt. George Dixon. Hide Caption 9 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – A wallet found in the shipwreck helps tells the story of ordinary life onshore. Hide Caption 10 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Crew member James Wicks' bandana after conservation. Hide Caption 11 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – This burnt matchstick was found buried in the sediment-filled interior of the H.L. Hunley. Hide Caption 12 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – Experts believe this lantern was used as a flashlight by the submarine's commander. Hide Caption 13 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – This conserved oil can almost looks good as new. Hide Caption 14 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Confederate submarine H. L. Hunley – The bowl of a pipe belonging to Confederate sailor Joseph Ridgaway, the only crew member positively identified through DNA. Hide Caption 15 of 16 Photos: Delving into mysteries of submarine H.L. Hunley Delving into mysteries of submarine H.L. Hunley – Experts examine a shoe found in the H.L. Hunley. Hide Caption 16 of 16

But Lance, who is working on a book about the Hunley, said that she has doubts about inconsistencies in these testimonies.

It is virtually impossible to know how powerful the Hunley's torpedo blast was, even with the amount of black powder used. The blast can also change with how tightly the powder is packed and how fine the grains are, according to the Naval History and Heritage Command. Replicating the black powder explosion, Lance said, was the trickiest part of the experiment.

So Lance lowballed it, testing several blasts in the process. She concluded that the shock wave would have instantly killed those aboard the Hunley, based on her calculations and a wealth of prior air blast experiments on large animals.

"Any explosive we've seen in the field ... would definitely create a lethal wave," Lance said.

"These types of injuries are not subtle," she added. "The damage is immediate."

Tick tock

There was another piece of evidence that stood in her favor: a gold pocket watch that belonged to the Hunley's captain, Lt. George Dixon.

The watch had stopped at 8:23, about the time of the Hunley's attack, historians believe.

"Most importantly, it appears it didn't wind down naturally," according to a 2007 update by a research partnership known as the Hunley Project. "Something traumatic -- perhaps water, a shock wave, or some other intervening force -- caused it to stop at that precise time."

Friends of the Hunley -- part of the Hunley Project, which was not involved in the new research -- declined to comment on the research. The organization maintains and researches the original submarine.

Prior naval research has concluded that "neither phase of the explosion was severe enough ... to have significantly impacted Hunley."

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"We had a lot of submariners survive being depth-charged at very close quarters during WWII," said Paul Taylor, a spokesman at Naval History and Heritage Command. "You sort of wonder how they did OK, but supposedly the folks in the Hunley didn't."

The Navy researchers who have been examining the Hunley for over a decade declined to comment on Lance's study while their own research on the crew deaths is ongoing.

But Lance, for one, said she feels like this part of the mystery has been solved.

"This project was originally intended to be a side project, and then it spiraled out of control when we realized we could do actually do it," she said.