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Canon 6D, Canon EF 100-400L ƒ4.5-5.6 IS USM, at 400mm, ISO250, 1/800, ƒ/6.3, Flash Yongnuo YN-560II at 105mm, 1/1 fired.

The flash did give a nice sparkle in the eye and overall scene backlit, isolating the tit itself.