The Kyle and Jackie O radio show has been suspended indefinitely after last week's lie detector stunt, with host Kyle Sandilands declaring he is unable to go on air.

There have been calls for 2Day FM's Kyle and Jackie O breakfast show to be axed by the station's owners, Austereo, since Wednesday when a 14-year-old girl was strapped to a lie-detector and asked on air about her sexual history.

When her mother asked if she was sexually active the girl replied: "I've already told you the story about this ... and don't look at me and smile because it's not funny."