The conservative firebrand credited with pressuring then-Speaker John Boehner John Andrew BoehnerLongtime House parliamentarian to step down Five things we learned from this year's primaries Bad blood between Pelosi, Meadows complicates coronavirus talks MORE (R-Ohio) to resign in 2015 issued a warning Thursday to Senate Majority Leader Mitch McConnell Addison (Mitch) Mitchell McConnellMomentum growing among Republicans for Supreme Court vote before Election Day Trump expects to nominate woman to replace Ginsburg next week Video of Lindsey Graham arguing against nominating a Supreme Court justice in an election year goes viral MORE (R-Ky.) and his establishment allies.

“Anytime leadership goes after Freedom Caucus members, it’s not the prudent thing to do,” Rep. Mark Meadows (R-N.C.), chairman of the far-right Freedom Caucus, told reporters in the Speaker’s lobby.

Meadows was referring to a television ad funded by the McConnell-linked Senate Leadership Fund (SLF), that attacked Freedom Caucus member Rep. Mo Brooks Morris (Mo) Jackson BrooksOvernight Defense: Trump hosts Israel, UAE, Bahrain for historic signing l Air Force reveals it secretly built and flew new fighter jet l Coronavirus creates delay in Pentagon research for alternative to 'forever chemicals' OVERNIGHT ENERGY: House Democrats tee up vote on climate-focused energy bill next week | EPA reappoints controversial leader to air quality advisory committee | Coronavirus creates delay in Pentagon research for alternative to 'forever chemicals' Coronavirus creates delay in Pentagon research for alternative to 'forever chemicals,' official says MORE (R-Ala.) as a “career politician” who has teamed up with liberals Rep. Nancy Pelosi (D-Calif.) and Sen. Elizabeth Warren Elizabeth WarrenBiden's fiscal program: What is the likely market impact? Warren, Schumer introduce plan for next president to cancel ,000 in student debt The Hill's 12:30 Report - Presented by Facebook - Don't expect a government check anytime soon MORE (D-Mass.) in attacking President Trump Donald John TrumpUS reimposes UN sanctions on Iran amid increasing tensions Jeff Flake: Republicans 'should hold the same position' on SCOTUS vacancy as 2016 Trump supporters chant 'Fill that seat' at North Carolina rally MORE.

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The ad from the McConnell-aligned super PAC uses video clips of Brooks criticizing Trump during the 2016 presidential primary.

“I don’t think you can trust Donald Trump with anything he says,” the congressman says in the ad, adding that those who vote for Trump will regret their decision.

Brooks, regarded as one of the most conservative House members, is challenging new GOP Sen. Luther Strange, the former Alabama attorney general who was appointed in February to replace Jeff Sessions after he was confirmed as Trump’s attorney general.

McConnell and his allies, including the SLF, are backing Strange in the Aug. 15 special Senate primary. And they believe Brooks’ past criticisms of Trump, who’s enormously popular in Alabama, will severely harm his Senate ambitions.

But Meadows suggested the SLF is attacking Brooks so aggressively in ads and news releases because the congressman is rising in the polls.

“Of course he’s a viable candidate. I think he wins,” Meadows predicted.

Two years ago this month, the North Carolina conservative stunned Washington by filing a “motion to vacate” resolution to try to oust Boehner John Andrew BoehnerLongtime House parliamentarian to step down Five things we learned from this year's primaries Bad blood between Pelosi, Meadows complicates coronavirus talks MORE from the Speakership. That move, on Meadows’ birthday, came after Boehner had retaliated against several Freedom Caucus members, and it triggered a series of actions that led to Boehner’s resignation just two months later, in the middle of his third term as Speaker.

When reporters jokingly suggested he could present a "motion to vacate" in the Senate, Meadows replied that there is no procedural maneuver to remove a sitting Senate majority leader.

“It’s not fun to joke about those things in July, but I do know there is no vehicle over there,” Meadows said.