Police have charged Gold Coast millionaire John Chardon, 69, with the alleged murder of his 34-year-old wife Novy, who disappeared more than three years ago from the Mt Nathan mansion they shared.

Chardon, a multi-millionaire industrial lubricants manufacturer, was handcuffed and taken into police custody after an investigation which included two separate searches for his fifth wife's remains.

He was charged with the murder of the mother of two and was to face the Brisbane Magistrates Court later on Friday.

Detectives and forensic officers searched the Chardon mansion in the Gold Coast hinterland following her February 2013 disappearance and the publication of her last sighting, on CCTV in a Gold Coast service station ten minutes from her home.

The search including unearthing a white vehicle police feared may have been buried below ground with the entombed woman's remains.

In January last year, police divers searched dams on a Gold Coast property at Advancetown belonging to the mother of pop star Rikki Lee Coulter for the body of Ms Chardon.

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Murder charge: Gold Coast millionaire John Chardon, pictured handcuffed in a police car, has been arrested and charged with the murder of his fifth wife, Novy

Indonesian born Novy Chardon, 34, (pictured) disappeared from the Gold Coast mansion she ahred with her multi-millionaire husband John Chardon in January 2013 and has never been seen again

Multi millionaire John Chardon, pictured in a police car after being taken into custody for his wife's alleged murder, had argued with her over property and a divorce prior to her disappearance, the missing woman's mother claimed

Novy Chardon, 34, (pictured, left) vanished in early 2013 from the Gold Coast property owned by her millionaire husband John (right), who has now been arrested and charged with killing her

During the first search of the Mt Nathan property, John Chardon was absent and overseas.

Just two days after his wife disappeared, Chardon took their two young children on a pre-planned business trip to Indonesia. He has always maintained his innocence of her murder.

He remained overseas for several weeks and at the time said Mrs Chardon had gone missing before and denied playing any part in her disappearance, despite previously being named in court as the chief suspect.

A major Liberal National party donor, John Chardon was once praised in the Queensland state parliament and was the principal of Candan Industries lubricant suppliers.

Last sighting: One of the last images of Novy Chardon, 34, on CCTV at an Upper Coomera service station on the Gold Coast in early 2013, before she vanished and police began digging her husband's property for her remains

Police divers made a second intense search for the body of missing multi-millionaire's wife Novy Chardon at the property of TV star Rikki Lee Coulter's (above, left) mother last year in Advancetown (above right)

Family mansion: This is the Mt Nathan mansion west of the Gold Coast where Novy Chardon lived with her 68-year-old husband before she vanished and detectives turned up with earthmovers to excavate

Police excavated (above) the Mt Nathan property of industrial lubricants millionaire John Chardon, 68, last year of search of remains of his missing 34-year-old Indonesian-born wife Novy

Police and about 50 SES volunteers made afuritlesds search in January 2015 of the Advancetown property, just 17km south of Chardon's former Mt Nathan home.

Regional crime co-ordinator Superintendent Dave Hutchinson said at the time that the operation was a body search. 'We're searching for Novy's remains', he told the Gold Coast Bulletin.

Supt Hutchinson later said that no items of interest had been located, but the investigation would continue.

'Although we came up empty-handed today, our determination to solve this case remains as strong as ever,' he said.

After Ms Chardon vanished three years ago, her car was found dumped several suburbs away near Nerang Train Station.

No longer alive: Detectives admitted they were looking for the body of missing Novy Chardon (above) and that they believed she was no longer alive

Jailed: Industrial lubricants millionaire John Chardon, 68, (above) has been arrested and charged with murder more than three years after his fifth wife's disappearance