Not one but five knockouts cost a pro fighter his purse — stuffed with $155,000 in cash and jewelry — at a Flatiron nightclub, police said Sunday.

Professional pugilist Jermall Charlo, 27, was partying with his assistant and the femmes fatale at Jay-Z’s 40/40 club in Manhattan early Sunday when he got into a “physical altercation” with someone around 3 a.m., according to police sources.

The assistant handed one of the women the bag — containing $40,000 in cash and $115,000 worth of bling — to hold during the unsanctioned sparring match, sources said.

But when the tiff ended and the Texan light middleweight returned to his seat, he learned the woman had absconded with his pricey pack along with her four pals, according to cops.

Charlo, who boasts a 26-0 record in the ring, had been at a boxing show at Brooklyn’s Barclays Center earlier Saturday night, according to a Boxing Scene report. He had been scheduled to fight Hugo Centeno Jr., but their bout was postponed due to Centeno suffering a rib injury.

Police are now on the hunt for the bag thief and her entourage, who were all caught on security camera leaving the club.

Cops describe the main bag-snatch suspect as a white woman with straight blond hair, around 5 feet 10 and 165 pounds. She was last seen wearing a dress, a jacket and carrying a blue bag.