Three high school female track athletes in Connecticut filed a federal discrimination complaint with the Department of Education about having to compete against transgender athletes.

A statement from Alliance Defending Freedom, a conservative Christian nonprofit organization, said it had filed the complaint on behalf of the three students, stating that transgender athletes had deprived female athletes of honors and opportunities to compete at elite levels.

Transgender athletes are allowed to compete in women's sports under a policy from the Connecticut Interscholastic Athletic Conference (CIAC), according to the ADF

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The policy is "grossly unfair and destroys" athletic opportunities, said ADF Legal Counsel Christiana Holcomb in a statement.

The CIAC told The Hill in a statement that it would "cooperate fully" with any investigation, while noting it had not been contacted by the Office of Civil Rights of the Education Department.

"The CIAC is committed to equity in providing opportunities to student athletes in Connecticut,” Lungarini said in a written statement to the Hartford Courant. “We take such matters seriously, and we believe that the current CIAC policy is appropriate under both Connecticut law and Title IX.”

"We take such matters seriously, and we believe that the current CIAC policy is appropriate under both Connecticut law and Title IX," CIAC Executive Director Glenn Lungarini said in a statement.

The lawsuit comes at a time when transgender rights are being challenged. President Trump Donald John TrumpSteele Dossier sub-source was subject of FBI counterintelligence probe Pelosi slams Trump executive order on pre-existing conditions: It 'isn't worth the paper it's signed on' Trump 'no longer angry' at Romney because of Supreme Court stance MORE's order banning transgender people from serving in the military took effect in March.

ADF also opposes same-sex marriage and does not believe transgender people should be protected from anti-discrimination laws.

Recently ADF represented a group of unnamed students challenging a transgender bathroom policy in a Pennsylvania school district. The Supreme Court declined to take up the challenge in May allowing the policy to go into effect.