The chief of staff to Motoring Enthusiast Party senator Ricky Muir has quit, just two months after taking up the role.

Sarah Mennie resigned on Friday but declined to comment on the circumstances surrounding the move.

Ms Mennie took on the role in August after Senator Muir's first chief of staff, Glenn Druery, was sacked.

Another staffer, Peter Breen, was also sacked about the same time.

The ABC has contacted Senator Muir's office for comment.

Division within the Motoring Enthusiast Party came to a head after last year's elections, when the party's Victorian council was sacked after being accused of acting against the best interests of the senator.

The Age newspaper reported the Victorian branch had voted to disband, creating uncertainty over whether Senator Muir would have to stand as an independent.

Party leader Keith Littler said the branch was "a bunch of disgruntled people" who had waged "a campaign of disrespect against the party".

Mr Druery, dubbed "the preference whisperer", was sacked by Senator Muir in September this year, fuelling rumours of further infighting within the party.

He was informed of his termination via an email which said he did not get on with staff.

But he said he suspected that he was sacked because Mr Littler wanted to be a senator.