a–f, Analysis of the frequencies of haematopoietic stem and progenitor cell populations in the bone marrow of 10–12-week-old Mx1-Cre;Tet2fl/fl;Flt3ITD mice and littermate controls three weeks after poly(I:C) treatment (12–17 independent experiments; the numbers of mice per treatment in a–c are shown across the top of a and for d–f across the top of d). g, Percentage of donor-derived haematopoietic cells after competitive transplantation of 300,000 donor bone marrow cells of the indicated genotypes along with 300,000 competing recipient cells in irradiated recipient mice (a total of 2–5 donor mice and 6–20 recipient mice per treatment from five independent experiments). h, Secondary transplantation of 5 million bone marrow cells from primary Gulo−/− (ascorbate-depleted) recipients of Flt3ITD/+ cells in irradiated wild-type or ascorbate-depleted Gulo−/− recipient mice (a total of three donor mice and 10–18 recipient mice per treatment from three independent experiments). *Comparison to wild type and #comparison to Flt3ITD/+. i, Ascorbate levels in donor cells of the indicated genotypes sorted from transplant recipients (a total of n = 4 mice per condition from 2 independent experiments). 1°, primary transplantation; 2°, secondary transplantation. j, Frequencies of donor cells of the indicated genotypes in the blood of transplant recipients described in Fig. 3f, g. k, Analysis of HSC frequency in the fetal liver of embryonic day (E)17.5 mice of the indicated genotypes (five independent experiments; the numbers of mice per treatment are shown across the top). All data represent mean ± s.d. Statistical significance was assessed with one-way ANOVAs followed by Fisher’s LSD tests (d, k) or Kruskal–Wallis tests (a–c, e). In other cases, we used two-way ANOVAs followed by Fisher’s LSD tests (i), Kruskal–Wallis tests (j) or a non-parametric mixed model followed by Kruskal–Wallis tests for individual time points (g, h). We corrected for multiple comparisons by controlling the false discovery rate. *P < 0.05, **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001; +P < 0.05, ++P < 0.01, +++P < 0.001. *Comparison to the wild-type (+/+) condition, #comparison to the Flt3ITD condition and +comparison to the Tet2Δ/+ or Tet2Δ/Δ conditions. To simplify the representation of statistical significance in g, statistical significance was only noted when both Tet2Δ/+ and Tet2Δ/Δ conditions, or both Tet2Δ/+;Flt3ITD and Tet2Δ/Δ;Flt3ITD conditions, were significantly different from other conditions). Source data