Eric Weissberg, a gifted multi-instrumentalist whose melodic banjo work on the 1973 hit single “Dueling Banjos” helped bring bluegrass music into the cultural mainstream, died on Sunday in a nursing home near Detroit. He was 80.

Juliet Weissberg, his wife of 34 years, said the cause was complications of Alzheimer’s disease.

Though the theme songs to the film “Bonnie & Clyde” (1967) and the CBS sitcom “The Beverly Hillbillies,” both recorded by Flatt and Scruggs, preceded “Dueling Banjos” in exposing wide audiences to bluegrass, neither made it to the pop Top 40. “Dueling Banjos,” which appeared on the soundtrack to the 1972 movie “Deliverance,” fared far better, rising to No. 2 on the Billboard pop chart.

The soundtrack to “Deliverance” was also certified gold, for sales of more than 500,000 copies.

But Mr. Weissberg — who also played fiddle, mandolin and guitar — produced much more than a one-hit wonder. More than a decade before “Dueling Banjos,” he had distinguished himself as a member of two popular folk groups, the Greenbriar Boys and the Tarriers, and as an in-demand session musician in New York.

As a session player he appeared on Judy Collins’s “Fifth Album,” contributing guitar to her 1965 version of “Pack Up Your Sorrows.” He played banjo on John Denver’s 1971 Top 10 pop hit, “Take Me Home, Country Roads.” His fretwork was heard on albums like Bob Dylan’s “Blood on the Tracks” (1974), Billy Joel’s “Piano Man” (1973) and the Talking Heads’ “Little Creatures” (1985). He collaborated with jazz musicians like Bob James and Herbie Mann as well.