Isaac Rotenberg was born to Natan and Miriam Rotenberg in Poland on 15 March 1927. A selektzia was held in his city following the outbreak of the Second World War, and his family was sent to the Sobibór extermination camp. With the exception of him, his younger brother, and his sister, his entire family perished. He was taken with his brother to a labor camp. When a revolt broke out, the two succeeded in escaping the camp, but they lost track of each other in the ensuing commotion. Isaac then made his way to the forest and joined the partisans.

In April 1947, Isaac reached the Land of Israel. He joined the IDF the next year, and fought in the War of Liberation in the north, near Kibbutz Manara.

Isaac was married to Riva, and they had two children, Tzipora and Pinhas. He worked as a plasterer, and was a founder of the city of Holon. Upon reaching retirement age, he decided to continue working a few hours per day to keep himself busy.

On 29 March 1994, during the Passover holiday, as Isaac was hunched on his knees, fixing a floor in his workplace in Petah Tikva, two of the Arab laborers on site attacked him and struck the back of his neck with axes. He was critically wounded, and entered a coma. Two days later, on 31 March, he died.

The murderers were caught staying in Lod with their Israeli Arab accomplices, and were sentenced to life in prison.

Isaac was 67 at the time of his death. He was survived by his wife, son, brother, and sister.