Drug repositioning, finding new applications for known drugs, expedites the drug development process by obviating the need for early toxicity trials. The Connectivity Map (CMap) is a database of gene expression profiles from varying human cell lines responding to treatment with different compounds, including FDA‐approved drugs and nondrug bioactive compounds, which can be used to predict potential small‐molecule candidates (Lamb et al ., 2006 ). In this project, we hypothesized that drugs that induce a transcriptional profile in human cell lines overlapping with that of CR are likely to act as CR mimetics. Therefore, we cross‐linked mammalian molecular signatures of CR with drug signatures available in the Connectivity Map and obtained various candidates. Subsequently, we employed lifespan and healthspan assays to test the ability of CR mimetic candidates to induce a CR‐like state in C. elegans and found new potential CR mimetics, demonstrating that our method can uncover biologically relevant findings.

A number of pathways have been already associated with CR and hence are likely targets of CR mimetics. Target of rapamycin (TOR) is a well‐documented nutrient sensor that regulates metabolism and growth in response to amino acid and nutrient intake (Wullschleger et al ., 2006 ; Fontana et al ., 2010 ). TOR is inhibited by rapamycin, resulting in an increased lifespan in organisms from yeast to mammals (Stanfel et al ., 2009 ; Miller et al ., 2011 ). RNAi of TOR increased lifespan in wild‐type C. elegans , but not in eat‐2 mutants, a long‐lived CR genetic model (Hansen et al ., 2007 ).

The aging of the population has created an urgent need to develop approaches targeting the aging process. In model organisms, aging can be delayed by genetic manipulations and by nutritional interventions like caloric restriction (CR), which consists of restricting calorie intake without malnutrition (Fontana et al ., 2010 ; de Magalhães et al ., 2012 ). CR is not a perfect solution and comes with several side effects (Dirks & Leeuwenburgh, 2006 ). Therefore, CR is unlikely to be widely adopted by the public. As such, developing CR mimetics, compounds that reproduce the longevity benefits of CR without its side effects, is of widespread interest.

Lastly, we aimed to assess whether the pattern produced by allantoin or rapamycin in N2 worms is similar to that of eat‐2 controls. Allantoin‐treated N2 worms have ~600 DEGs (346 up, 243 down) compared to eat‐2 controls, while rapamycin changes more than 3500 genes (1487 up, 2178 down). Comparing the relative changes caused by allantoin in N2 worms with the changes between eat‐2 and N2, there is a significant overlap among downregulated genes (16 genes, Fisher's one‐sided, P = 4.69E‐14), but not for upregulated genes. Contrarily, rapamycin‐induced expression changes that overlap with eat‐2 vs. N2 changes have predominantly opposite directions (56 genes; P < 2.59E‐08).

Next, we wished to determine whether the lack of lifespan effect of rapamycin in eat‐2 mutants can be seen at a transcriptional level. The functional comparison of eat‐2 and N2 controls revealed only a few enriched terms (body morphogenesis, sphingolipid metabolism, lipase activity and aspartic‐type peptidase activity; Table S5), in line with the low number of DEGs (Table 4 ). Still, the profile of rapamycin‐treated eat‐2 worms shows a smaller number of DEGs (238 up, 312 down), a result very different than for rapamycin‐treated N2 worms (>1500 DEGs). While there is a fair amount of overlap between the two rapamycin profiles: 44 genes in the same direction changes (up: P = 3.39E‐5; down: P = 1.10E‐3), there are also 35 genes with opposite changes (more than expected by chance). In contrast to the functional analysis in the rapamycin‐treated N2 worms, in the eat‐2 background a much smaller number of terms are enriched. Interestingly, one of the functional categories enriched with downregulated genes is regulation of growth and determination of adult lifespan, although we have not observed any noticeable phenotype in the treated eat‐2 worms.

First, we wanted to determine whether the drugs affect a large portion of genes in N2 worms. Feeding allantoin or rapamycin to worms results in several hundred genes being differently expressed, both up‐ and downregulated, with rapamycin having a wider effect. This suggests that the effect of both drugs is not localized to a small set of targeted genes, but results in widespread changes. Interestingly, the signatures produced by rapamycin and allantoin are drastically different. While allantoin downregulates many genes (almost 80%), for rapamycin more than 55% of the affected genes are upregulated. Furthermore, the overlap of genes changing in the same direction is low (upregulated genes: not significant, Fisher's one‐sided, P = 0.37; downregulated genes: lower than expected, P = 1.6E‐3). The difference can also be observed in terms of functional enrichment (Table S5). For allantoin, we found only that ‘neuropeptide signalling pathway’ and ‘fatty acid metabolism’ are enriched in downregulated genes, while rapamycin affects several biological processes: moulting cycle, dephosphorylation, regulation of multicellular organism growth, cuticle development and body morphogenesis are enriched in upregulated genes, and embryonic development, cell cycle and nuclear division, cell fate, cellular response to various stresses, negative regulation of metabolic processes and biosynthesis, TGFb and WNT signalling are all enriched in downregulated genes. Many of these findings (e.g. downregulation of cell cycle‐related terms) are in accordance with current knowledge regarding the effects of rapamycin. While our analysis focused mostly on biological processes and pathways, both up‐ and downregulated genes included previously established LAGs, which is in line with the above results from potential drug targets that suggest effects at the level of the longevity network (Table S6).

Rapamycin, thought to be a CR mimetic, is one of the most studied drugs, and understanding this compound better is essential to better understanding CR. To this end, we decided to evaluate gene expression changes in both eat‐2‐ and N2‐treated worms. Allantoin, on the other hand, has not been studied in terms of aging or CR. As such, its analysis in N2 worms is also important to understand whether it acts similarly to rapamycin and/or to CR in eat‐2 mutants.

The worm longevity network. The network (which includes longevity‐associated genes and their interaction protein partners) contains many potential links to drug targets through which allantoin, LY‐294002, rapamycin and trichostatin A might have an effect on its functionality. For an integrative visualization, all drug targets have been displayed (including the targets that do not belong to the network).

As previously shown, all longevity‐associated genes (LAGs), together with their direct interaction partners, form a continuous protein–protein interaction network. This worm longevity network was successfully used to predict new longevity regulators, and we have shown that the first‐order interactors of known worm LAGs are also likely to participate in the regulation of lifespan (Tacutu et al ., 2012 ). As such, it is interesting to know how many more non‐LAG targets belong to the worm longevity network (Fig. 6 ). Of the 11 additional targets, 8 have multiple interactions, some being quite central. For example, hda‐1 , one of TSA targets, interacts with 15 other network genes, and gsy‐1 and pcn‐1 , both targets of rapamycin, interact with 11 and 9 other genes, respectively. This suggests that TSA and rapamycin might modulate lifespan in a systemic way. While allantoin and LY‐294002 are more peripheral in the network, some of their targets are also not very far from central nodes and LAGs. Thus, although the targets for the drugs hardly overlap, it seems that these four drugs perturb the longevity network, either by targeting specific genes or through effects on multiple genes that are different between the drugs, to modulate longevity.

Next, we aimed to understand the links between the targets of the drugs, longevity and CR (Fig. 5 ). Using the GenAge database (Tacutu et al ., 2013 ), we found among the drug targets genes previously associated with lifespan regulation in worms. For example, RNAi of let‐363 and rps‐6 (rapamycin and LY‐294002 targets), and RNAi of F55B11.1 (one of the predicted targets of allantoin), extends lifespan of N2 worms. In contrast, RNAi of atg‐5 and postdevelopmental RNAi of eef‐2 , both potential targets of rapamycin, result in a lifespan reduction. Other targets include age‐1 (target of LY‐294002), whose mutants are well known to be long lived, and sir‐2.1 (target of TSA) whose overexpression extends lifespan by up to 50%. Interestingly, let‐363 , rps‐6 and sir‐2.1 are also in GenDR as associated with CR longevity effects (Wuttke et al ., 2012 ). Additionally, we found three genes so far not associated with longevity which have been suggested to play a role in CR: orthologs of fkb‐6 and gsy‐1 (both targets of rapamycin) are significantly overexpressed in mice under CR (Plank et al ., 2012 ). Additionally, cbp‐1 (a transcriptional factor that might be affected by TSA) is induced under CR and blocking it results in countering the protective effects of CR (Zhang et al ., 2009 ).

To explore the mechanisms and pathways by which the tested drugs mimic CR, we looked at their predicted targets. Using STITCH (Kuhn et al ., 2012 ), we identified 5 gene targets for allantoin, 19 for rapamycin, 5 for LY‐294002 and 9 targets for TSA. Although the overlap between the four target sets was minimal, with only 2 genes being common targets for rapamycin and for LY‐294002 ( let‐363 , a homologue of mTOR , and rps‐6 ), it is still possible that all mechanisms converge to a downstream signature. This idea is further supported by our MANTRA (Carrella et al ., 2014 ) analysis, which allows the visualization of the similarity in induced gene expression for a set of drugs using a network (Fig. S17). This analysis showed that geldanamycin, rapamycin and LY‐294002 act in a relatively similar manner, with TSA having a more distant profile. Allantoin seems to act in a manner distinct from the rest of the compounds as it appears unconnected to the other compounds (Fig. S17).

The movement rate of either N2 control worms or those treated with geldanamycin. The movement rate was recorded on days 1, 5, 10 and 15 post‐L4 moult when treatment with the drug began. The geldanamycin‐treated worms show a much faster initial decline in movement compared to the untreated controls before levelling out after day 5 with no decrease between days 5 and 10. Movement rate decreases faster in geldanamycin‐treated worms with the rate of movement at days 5, 10 and 15 for treated worms (2.8, 2.7 and 0 body bends per minute, respectively) being lower than the rate in untreated controls (10.9, 7.7 and 3.7 body bends per minute, respectively), to a significant degree (two‐tailed t ‐test) at day 5: ( P < 0.001); day 10: ( P < 0.001); day 15: ( P < 0.05). *Significance of at least 0.05; **significance of at least 0.005. Error bars indicate ±1 standard error.

Geldanamycin‐treated worms showed a faster decline of pharyngeal pumping rate compared to untreated controls. The rate of pumping in treated worms was lower at day 5 (Fig. S13). Movement rate decreased faster in geldanamycin‐treated worms (Fig. 4 ). The rate of this decline is much greater between days 1 and 5; however, the rate seems to level off. These results indicate that the worms are aging at a faster rate or are suffering some toxic effect of the drug, despite the lack of reduction in lifespan.

The pharyngeal pumping rate of either N2 control worms or those treated with allantoin (A), trichostatin A (B), rapamycin (C) or LY‐294002 (D). The pumping rate was recorded on days 1, 5, 10 and 15 post‐L4 moult when treatment with the drug began. The treated worms showed a slower decline of pharyngeal pumping compared to the untreated controls. The rates of pumping in allantoin‐ (83.5 pumps per minute), trichostatin A‐ (46.7 pumps per minute), rapamycin‐ (45.3 pumps per minute) and LY‐294002 (33.88 pumps per minute)‐treated worms are greater than those of untreated worms (25.3 pumps per minute) to a statistically significant degree at day 10 (two‐tailed t ‐test): allantoin ( P < 0.001, N = 10), trichostatin A ( P < 0.05, N = 10), rapamycin ( P < 0.05, N = 10) and LY‐294002 ( P < 0.01, N = 10). *Significance of at least 0.05. Error bars indicate ±1 standard error.

Worms treated with allantoin (Fig. 3 A), TSA (Fig. 3 B), rapamycin (Fig. 3 C) or LY‐294002 (Fig. 3 D) showed a significantly slower decline in pharyngeal pumping (Figs S13–S16) and a higher rate of pumping at day 10 when compared to control worms. This difference indicates that the drugs prolong lifespan through delayed aging with worms remaining healthier later in life. It also indicates that drugs are not inducing CR by restricting food intake as the worms’ pharyngeal pumping is not inhibited. At day 15, there is no significant difference between treated and control worms, indicating a faster decline in pumping after day 10 among the treated worms.

To determine whether the lifespan increase was also affecting healthspan, we measured the healthspan of treated wild‐type worms by performing both movement and pharyngeal pumping assays. Both worm movement and pharyngeal pumping rate have been shown to decrease with age (Hosono et al ., 1980 ), and so any delay in this decline would indicate a slower rate of aging. All the treated worms, except those treated with geldanamycin, showed no change in the decline of movement rate (Fig S4–S12).

DAF‐16 is not required for lifespan extension induced by CR (Houthoofda et al ., 2003 ), but is required for lifespan changes induced by perturbations of the DAF‐2 signalling pathway. If our candidate compounds are CR mimetics, we would expect to see a lifespan increase in daf‐16 mutant worms. Therefore, we analysed the lifespan of daf‐16 worms treated with rapamycin, allantoin or TSA. The average lifespan of daf‐16 worms treated with rapamycin (21.8 days), allantoin (21.5 days) or TSA (22.2) was significantly greater than that of untreated daf‐16 worms (17.9 days) (Fig. 2 ), in line with our predictions that these are possible CR mimetics.

Overall, all four drugs predicted to mimic CR (allantoin, TSA, rapamycin and LY‐294002) showed an increase in lifespan in a CR‐like manner. Geldanamycin that showed an opposite gene expression to the above drugs and hence was expected to negate the effects of CR appeared to have no effect on longevity.

As eat‐2 worms eat less, it is possible that they were simply not taking in enough of the drugs to increase lifespan. To this end, we then tested worms with a double dosage for the most novel drugs: allantoin or TSA (Figs S2 and S3). As before, a significant increase in lifespan was observed for all drugs in N2 controls (>20% increase in lifespan, except for the higher allantoin dosage which was only 14%). A statistically significant increase in lifespan was observed when drugs were administered to eat‐2 worms; however, the effect sizes were very small (<5% increase) and this was observed for both dosages in this trial, suggesting that the much greater lifespan increase in N2 worms is not due to eat‐2 worms taking up less drug.

Percentage survival of wild‐type (N2) ormutant worms alone or treated with allantoin (A), trichostatin A (B), rapamycin (C) or LY‐294002 (D). Worms that did not respond to touch stimulation were recorded as dead. Bothand treated worms of both genotypes showed both a significantly longer lifespan than N2 controls and no significant variation from each other (Table 3 ). (A) Allantoin‐treated N2 had a 21.9% increase in lifespan. (B) Trichostatin A‐treated N2 had a 22.1% increase in lifespan. (C) Rapamycin‐treated N2 had an 18.9% increase in lifespan. (D) LY‐294002‐treated N2 had a 21.6% increase in lifespan. Results are shown for combined data from two representative trials (full range of results is shown in Table S3).

Allantoin‐ (Fig. 1 A), TSA‐ (Fig. 1 B), rapamycin‐ (Fig. 1 C) and LY‐294002‐treated (Fig. 1 D) N2 worms showed a prolonged lifespan compared to N2 controls. The average lifespan of N2 worms treated with allantoin (28.3 days), TSA (28.4 days), rapamycin (27.6 days) and LY‐294002 (28.1 days) was significantly greater than that of untreated controls (23.1 days) (Table 3 ). This shows that the drugs are sufficient to increase lifespan of wild‐type worms, although the lifespan extension observed in each treatment showed some variation between trials (Table S3). No consistent increase in lifespan was detected in the treated eat‐2 worms when compared to eat‐2 controls or to N2 worms treated with the drugs (Fig. 1 ), although small effects were occasionally observed (Table S3). Taken together, these results indicate that allantoin, TSA, rapamycin and LY‐294002 extend lifespan through a mechanism potentially similar to CR. The exception was geldanamycin‐treated worms (Fig. S1) that did not show any lifespan increase and did not differ significantly from untreated controls of the same genotype. An initial trial with geldanamycin in eat‐2 worms showed a reduction in lifespan, as if geldanamycin was negating the effect of CR (Table S3). This was not reproduced in further trials.

We selected 5 drugs identified as inducing similar or opposite transcriptional profiles to CR to analyse further. These compounds were selected based on different criteria: rapamycin, LY‐294002 and trichostatin A (TSA) because they are already known to increase lifespan; TSA was the top candidate from specific dosages after rapamycin (Table 2 ); allantoin was chosen as it was a compound not studied previously in the context of aging; and geldanamycin was selected as it induces a transcriptional profile opposite to that of rapamycin and hence would appear to undo CR. To test the selected drugs’ capability to extend lifespan, we performed lifespan assays of wild‐type worms exposed to each drug from the final stage of larval development (after L4 moult, 2 days posthatching). To evaluate whether the mechanism of action of these drugs is similar to that of CR, they were also tested in long‐lived eat‐2 mutants, as they have a lower food uptake and hence a constant CR‐like state. If these drugs acted through a mechanism different from CR, then we would expect a synergistic increase in lifespan in the eat‐2 worms. Contrarily, for similar mechanisms, we would expect diminished or no lifespan effects in treated eat‐2 worms.

To identify potential CR mimetic drugs, we retrieved a CR transcriptional profile from published data in rat cells exposed to sera from rats or rhesus monkeys undergoing CR (de Cabo et al ., 2003 ). The transcriptional profile was used to query the CMap to identify drugs that induce similar or opposite profiles (see 5 ). This analysis provided both a list of drugs that match the profile of CR based on all data in the CMap (Table 1 ) and a list based on different drug dosages for each drug (Table 2 ). Overall, we discovered 11 small molecules significantly inducing gene expression changes overlapping with those induced by CR. Of note, rapamycin was one of our top hits and is known to extend lifespan in organisms from yeast to mice (Stanfel et al ., 2009 ; Miller et al ., 2011 ). We also found LY‐294002 to be significant and wortmannin close to significance (Table S2). These molecules also extend lifespan in invertebrates through the inhibition of the PI3K pathway (Babar et al ., 1999 ).

Discussion

The fact that rapamycin was one of the top results among over 1000 drugs tested using our drug repositioning approach is a strong indicator that this bioinformatics approach provides biologically relevant results. This is further strengthened by the presence of other known life‐extending compounds such as LY‐294002 and wortmannin (Babar et al., 1999). We have shown that rapamycin, LY‐294002, TSA and allantoin can increase lifespan in C. elegans in a manner that does not act synergistically with CR‐induced lifespan extension, indicating that these compounds and CR may act through a similar mechanism. Additionally, we have shown that this lifespan extension comes with a possible slowing of aging seen through a moderately slower decline in pharyngeal pumping rate, but with no change in movement rate decline.

Previous studies have shown that rapamycin increases lifespan of N2 worms by 19% (Robida‐Stubbs et al., 2012), which is similar to our results despite their use of 10 times the dose used in this study (100 μm). These results help indicate the reliability of the data collected. Rapamycin acts via the TOR pathway (Stanfel et al., 2009), which is thought as crucial in CR. DAF‐16 is not required for the lifespan increase induced by rapamycin (Fig. 4), as expected.

LY‐294002 is a known inhibitor of phosphatidylinositol 3‐kinase (age‐1) and has been shown to increase lifespan in adult worms by ~10% (Babar et al., 1999). This is slightly lower than observed in the present study. The difference between our study and the Babar et al.'s research could be the result of a higher dose of LY‐294002 at 1.6 mm by Babar et al., which may be having a toxic effect. This is supported by Barbar et al.'s use of a higher dose at 2.4 mm, which showed no significant increase in lifespan. Mutations of age‐1 and daf‐2, which acts upstream of age‐1, result in lifespan extension in N2 worms, but daf‐2 mutations and RNAi also increases the lifespan of eat‐2 worms, which suggests that age‐1 would do so also (Chen et al., 2007). This is the opposite of what we have found. This may be due to the drug treatment not completely inhibiting age‐1. AGE‐1 acts upstream of TOR, and hence, the connection between LY‐294002 treatment and CR becomes clear.

Allantoin has not been previously studied in terms of effects on aging. It has been shown to bind to imidazoline receptors in mammals and have beneficial effects on energy regulation, potentially through the activation of AMP kinase which could then act through the mTOR pathway (Chen et al., 2012). As C. elegans does not appear to contain any imidazoline receptors, it is unclear how allantoin produces the effects seen in this study. It is still possible that a similar pathway exists in worms acting through yet undiscovered receptors. As allantoin is likely produced in C. elegans as a by‐product of the reaction of uric acid and reactive oxygen species (Artyukhin et al., 2013), there is the potential for a feedback mechanism by which allantoin signals the presence of oxidative stress. This stress signal would then activate stress response pathways similar to those activated under CR to give the observed results. Our results show that DAF‐16 is not required for the lifespan increase induced by allantoin.

TSA is a well‐documented inhibitor of class 1 and 2 HDACs and hence has the potential to induce widespread epigenetic changes. TSA has been shown to extend lifespan in C. elegans by about 11% (Zhang et al., 2009), which is in line with our results (Table S3). One possible mechanism to explain TSA's lifespan extension effects is through the production of nitric oxide (NO). TSA has been shown chemically to produce NO when it is oxidized (Samuni et al., 2010) and hence could potentially produce NO in worms. C. elegans have been shown to live longer when fed NO‐producing bacteria in a process that results in hsp16 activation (Gusarov et al., 2013). Increases in the heat shock protein hsp16 have been shown to increase lifespan in C. elegans in a DAF‐16‐dependent manner (Walker & Lithgow, 2003). However, DAF‐16 is not required for the lifespan increase induced by TSA. As such, further mechanistic studies are warranted to identify how TSA increases lifespan.

At the given dose, geldanamycin was unable to affect lifespan of wild‐type or eat‐2 worms. However, N2 worms showed a significant increase in the rate of decline of both movement and pharyngeal pumping compared to untreated controls, suggesting that the drug is having a biological effect. Geldanamycin, unlike the other drugs in this study, was predicted to induce a transcriptional profile opposite to that of rapamycin. Another study showed that prolonged treatment of geldanamycin had detrimental effects on motility and is hence in line with our results (Skantar et al., 2005). Geldanamycin inhibits Hsp90, mutants of which have been shown to have muscle cell defects (Gaiser et al., 2011). One of our trials indicated that geldanamycin prevented the lifespan extension in eat‐2 worms; however, these results were not reproducible.

We have observed a correlation between increased lifespan and prolonged pharyngeal pumping in treated worms. However, movement rate was unaffected even in long‐lived worms with prolonged pharyngeal pumping. This indicates that either movement rate is a less sensitive measure of aging rate than pharyngeal pumping or that movement rate and pharyngeal pumping rate decline are delayed by two different mechanisms. Those drugs that delayed the decline of pharyngeal pumping did so in a manner that was only seen significantly at day 10. It is likely that such observation could not be seen at earlier time points because the pumping rate does not decline much until after day 5, so no difference could be seen between the control worms and the apparently slow‐aging treated worms. The reason why there is no observed difference after day 10 is unclear. Perhaps the controls are declining to the low level seen at day 15 earlier and the pumping rate is remaining constant, or that the long‐lived treated worms have a faster decline that starts later in life.

To address the possibility that eat‐2 worms are not taking up as much of the drugs as N2, we retested our most interesting drugs using a double dosage. Eat mutant worms have been shown to have a pharyngeal pumping rate of 20% that of wild‐type worms, but a more recent study measuring food uptake suggests that they consume 60–80% as much food as wild‐type worms (Gomez‐Amaro et al., 2015). As such, using double the drug concentrations should allow eat‐2 mutants to uptake at least as much drug as N2. Because we obtained equivalent results with a higher dosage, this suggests that our drugs may indeed be acting via CR mechanisms. Further dosages in worms and in other model organisms of all the identified drugs should be studied in future.

To obtain a better understanding of the changes that the studied drugs cause in worms, we analysed the differences in gene expression in response to allantoin and rapamycin. While both drugs affect large numbers of genes in the N2 strain, rapamycin has a much wider effect and the signatures produced by rapamycin and allantoin were quite different. Nevertheless, both drugs extend lifespan and, as can be seen from the worm longevity network (Fig. 6), interact with longevity‐associated genes and their partners. As such, it is possible that a common downstream mechanism for the two drugs exists, only triggered through different signals and pathways. Our analysis of potential targets of the four life‐extending drugs (Fig. 5) supports this view.

The number of rapamycin‐affected genes is much smaller in the eat‐2 background (Table 4), compared to the wild‐type N2, which probably reflects the lack of an observable phenotype. It is unclear, however, whether this is due to rapamycin having a background‐specific effect, or because eat‐2 mutants eat less, and thus, the dose of rapamycin is reduced as well. Neither allantoin nor rapamycin produced a gene expression pattern highly similar to that of eat‐2 controls. A potential caveat is that the effects at other drug dosages have not been investigated. Different doses can have different effects, as seen in a recent study using the supposed CR mimetic metformin, in which some doses increased N2 lifespan, but decreased eat‐2 lifespan (Onken & Driscoll, 2010). However, regardless of this caveat, we have shown that the drugs computationally predicted to act as CR mimetics seem to do so at the tested dosages.

Pathways identified to affect aging may or may not be conserved between the nematode worm C. elegans and mammals and physiological differences could also affect how the drugs act (de Magalhães, 2014). It is important then to test whether the effects of these drugs are conserved in mammals such as mice. While further experiments for the effect of these drugs in mammals are undoubtedly necessary, our experiment also provides some promising hints towards these mechanisms being evolutionary conserved. The initial gene expression profile used as input for CMap was obtained from rodent cells exposed to sera from animals (rats or rhesus monkeys) under CR and was then compared with drug‐induced expression profiles in human cells. Testing these drugs in worms showed an extended longevity phenotype, suggesting that their CR mimetic effects may be evolutionary conserved.