New Biological Scaffold Home, Sweet Home, for Stem Cells

Highly aligned nano-fibers created by fibroblasts form a biological scaffold which could prove an ideal foundation for engineered tissues. Stem cells placed on the scaffold thrived, and it had the added advantage of provoking a very low immune response.

Our cells don’t live in a vacuum. They are surrounded by a complex, nurturing matrix that is essential for many biological functions, including growth and healing.

In all multicellular organisms, including people, cells make their own extracellular matrix. But in the lab, scientists attempting to grow tissue must provide a scaffold for cells to latch onto as they grow and proliferate. This engineered tissue has potential to repair or replace virtually any part of our bodies.

Typically, researchers construct scaffolds from synthetic materials or natural animal or human substances. All have their strengths and weaknesses, but no scaffolds grown in a Petri dish have been able to mimic the highly organized structure of the matrix made by living things, at least until now.

Feng Zhao of Michigan Technological University has persuaded fibroblasts, cells that makes the extracellular matrix, to make just such a well-organized scaffold. Its fibers are a mere 80 nanometers across, similar to fibers in a natural matrix. And, since her scaffold is made by cells, it is composed of the same intricate mix of all-natural proteins and sugars found in the body. Plus, its nanofibers are as highly aligned as freshly combed hair.

The trick was to orient the cells on a nano-grate that guided their growth—and the creation of the scaffold.

“The cells did the work,” Zhao said. “The material they made is quite uniform, and of course it is completely biological.”

Stem cells placed on her scaffold thrived, and it had the added advantage of provoking a very low immune response.

“We think this has great potential,” she said. “I think we could use this to engineer softer tissues, like skin, blood vessels and muscle.”

The work is described in the paper “Highly Aligned Nanofibrous Scaffold Derived from Decellularized Human Fibroblasts,” coauthored by Zhao, postdoctoral researcher Qi Xing and undergraduate Caleb Vogt of Michigan Technological University and Kam W. Leong of Duke University and published Jan. 29 in Advanced Funcational Materials. Zhao designed the project. Xing and Vogt did the work, and Leong developed the template for cell growth.

The work was funded by the National Institutes of Health (1R15HL115521-01A1), the Flight Attendant Medical Research Institute (062518-YCSA) and Michigan Technological University.

Michigan Technological University is a public research university, home to more than 7,000 students from 54 countries. Founded in 1885, the University offers more than 120 undergraduate and graduate degree programs in science and technology, engineering, forestry, business and economics, health professions, humanities, mathematics, and social sciences. Our campus in Michigan’s Upper Peninsula overlooks the Keweenaw Waterway and is just a few miles from Lake Superior.