Author notes

These authors contributed equally: Jie Yuan, Si-Yuan Chang, Shi-Gang Yin, Zhi-Yang Liu, Xiu Cheng

Affiliations

Institute of Neuroscience and State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China Jie Yuan, Si-Yuan Chang, Zhi-Yang Liu, Xi-Juan Liu, Qiang Jiang, Ge Gao, De-Ying Lin, Xin-Lei Kang, Shi-Wei Ye, Jiang-An Yin & Shi-Qing Cai CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China Shi-Gang Yin, Xiu Cheng, Pei Hao & Lubin Jiang University of Chinese Academy of Sciences, Beijing, China Jie Yuan, Si-Yuan Chang, Shi-Gang Yin, Zhi-Yang Liu, Xiu Cheng, Ge Gao & De-Ying Lin Academician (Expert) Workstation of Sichuan Province, The Affiliated Hospital of Southwest Medical University, Luzhou, China Shi-Gang Yin College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, China Zheng Chen School of Life Science and Technology, ShanghaiTech University, Shanghai, China Lubin Jiang Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, China Shi-Qing Cai

Contributions

J.Y. conducted HPLC assays, imaging assays and most of the behavioural assays; performed co-immunoprecipitation assays and western blots to detect the H3K9 methylation level in C. elegans; prepared the mRNA samples for RNA-seq; conducted experiments in mammalian cells; and analysed the gene-expression profile from human brain gene-expression datasets. J.Y. and X.-J.L. performed the genome-wide RNAi screen as well as lifespan and stress-resistance assays. S.-Y.C. conducted the RT–qPCR assays in C. elegans and the qPCR-based determination of mitochondrial DNA/nuclear DNA ratios; detected the ATP level and OCR in C. elegans; conducted UPRmt activation assays; and performed behavioural and lifespan assays related to UPRmt. S.-Y.C. and X.-J.L. performed the lifespan and H 2 O 2 stress resistance assays in mutants of different longevity pathways. X.-J.L. carried out assays of male mating behaviour. S.-G.Y. analysed the ChIP–seq data and the RNA-seq data of C. elegans, and helped to analyse gene-expression levels from human brain datasets. X.C. conducted the in vitro histone methyltransferase assay and ChIP–seq assays in C. elegans. Z.-Y.L. performed the co-immunoprecipitation and ChIP–qPCR assays in mammalian cells. Z.-Y.L. and S.-W.Y. measured the OCRs of isolated mitochondria from mice. G.G. carried out co-immunoprecipitation of SET-6 and BAZ-2. X.-L.K. and Z.C. helped to prepare samples for ChIP–seq assays in C. elegans. J.-A.Y. performed part of the assays for ATP detection in mouse neurons. Z.-Y.L. and D.-Y.L. performed mouse behavioural assays. Q.J. helped with the BSR and ESR assays. P.H. supervised the ChIP–seq data analysis. L.J. supervised experiments regarding epigenetics and analysis of the data. S.-Q.C. conceived the project. S.-Q.C., L. J., J.Y., S.-Y.C. and S.-G.Y. designed the study, analysed the data, and wrote the manuscript with input from all authors.

Corresponding authors

Correspondence to Lubin Jiang or Shi-Qing Cai.