An avid bike rider who started a 24-hour repair business for fellow cyclists was killed while riding through East Williamsburg early Saturday morning.

Matthew Von Ohlen, 35, was in a bike lane on Grand Street when he was hit by a black Chevrolet Camaro, police said.

The collision knocked him off his bicycle and caused severe trauma. He died at Bellevue Hospital.

A grainy video from a nearby restaurant shows a black car heading east on Grand Street, appearing to hit a bump, and driving on. A victim can then be seen lying in the road.

Gene Talmadge, 24, a dental student, who lives near the scene heard the collision and called 911 at 2:36 a.m.

“People crowded around him to protect him from on-going traffic,” Talmadge said. “They looked grim. He did not move.”

Talmadge said the driver who hit Von Ohlen stopped for a few minutes, but took off when sirens were heard.

Von Ohlen, a native of Minneapolis, lived near the accident scene.

Von Ohlen and partner Joseph Huba started Bikestock in 2013. The business installed vending machines with bicycle repair supplies and free air pumps in Brooklyn, Manhattan and Massachusetts.

Warning: Video contains graphic footage

The company’s web site said Von Ohlen “lives and breathes cycling.”

“I loved Matt like a brother,” Huba told The Post. “It’s really, really, really tough to lose someone like that.”

Von Ohlen, who had a degree in anthropology from Lawrence University in Wisconsin, also worked as a bartender at Apotheke in Manhattan.

No arrests have been made.

Additional reporting by Melissa Klein