We are happy to make accessible our ongoing work made in collaboration with the team of Prof. Gisou van der Goot of EPFL under the format of a BioRxiv preprint publication. Sound science takes time but awesome results must be shared as fast as possible!

It all started with a very talented Master student (Christopher Tremblay) who was shared between Nanolive and Prof. Van Der Goot’s lab and ended with this amazing work where it’s demonstrated, for the very first time, how the 3D Cell Explorer allows marker-free imaging of key cell organelles in live cells and much more!

Find the full text here: https://www.biorxiv.org/content/early/2018/09/04/407239

Abstract

Holo-tomographic microscopy (HTM) is a label-free non-phototoxic microscopy method reporting the fine changes of a cell’s refractive indexes (RI) in 3D. By combining HTM with epifluorescence, we demonstrate that cellular organelles such as Lipid droplets and mitochondria show a specific RI signature that distinguishes them with high resolution and contrast. We further show that HTM allows to follow in unprecedented ways the dynamics of mitochondria, lipid droplets as well as that of endocytic structures in live cells over long period of time, which led us to observe to our knowledge for the first time a global organelle spinning occurring before mitosis.