Introduction. Although there are historical records showing its existence for over 2,000 years, the so‐called female ejaculation is still a controversial phenomenon. A shared paradigm has been created that includes any fluid expulsion during sexual activities with the name of “female ejaculation.”

Aim. To demonstrate that the “real” female ejaculation and the “squirting or gushing” are two different phenomena.

Methods. Biochemical studies on female fluids expelled during orgasm.

Results. In this case report, we provided new biochemical evidences demonstrating that the clear and abundant fluid that is ejected in gushes (squirting) is different from the real female ejaculation. While the first has the features of diluted urines (density: 1,001.67 ± 2.89; urea: 417.0 ± 42.88 mg/dL; creatinine: 21.37 ± 4.16 mg/dL; uric acid: 10.37 ± 1.48 mg/dL), the second is biochemically comparable to some components of male semen (prostate‐specific antigen: 3.99 ± 0.60 × 103 ng/mL).

Conclusions. Female ejaculation and squirting/gushing are two different phenomena. The organs and the mechanisms that produce them are bona fide different. The real female ejaculation is the release of a very scanty, thick, and whitish fluid from the female prostate, while the squirting is the expulsion of a diluted fluid from the urinary bladder. Rubio‐Casillas A and Jannini EA. New insights from one case of female ejaculation. J Sex Med **;**:**–**.