Retailers have slammed the Queensland opposition over its plans to block a proposed overhaul of the state's trading hours.

The legislation is doomed to fail after key crossbenchers joined the Liberal National Party in opposing the Palaszczuk government bill, declaring the shakeup would hurt small and medium-sized businesses.

The National Retail Association issued a statement on Thursday lambasting the LNP for decided not to support the laws.

"The modern global retail market is moving at lightning pace, but Queensland retailers remain hamstrung by rules that were put in place nearly 30 years ago," NRA chief executive Dominique Lamb said.