Brisbane cyclists have staged a "die-in" on one of the city's busiest bridges to protest the proposed removal of dedicated bike lanes.

About 100 riders gathered at South Bank on Saturday morning before rolling onto Victoria Bridge's two car lanes to the strains of the Bee Gees' Stayin Alive.

They then held a minute's silence for cyclists who had been killed or injured on the roads before lying across the tarmac for the so-called "die-in".

Brisbane City Greens councillor Jonathan Sri said the protest was a "last resort" and stemmed from fears the proposed changes would lead to more conflict between pedestrians and cyclists.

"Everyone hates it when bikes and pedestrians get in each other's ways, he said.

"We're worried that by reducing cyclists' access on this bridge, that will force more along the South Bank riverfront and along the footpaths in the CBD."

While he didn't know the exact figures for 2017, Mr Sri said too many cyclists were dying or being injured on Brisbane roads.

Police and state government were also not accurately collecting data about the number of crashes involving cyclists, he claimed.

Before making their way onto the bridge, the Gabba Ward representative told protesters Brisbane had become too "car-centric" and was lagging behind other developed cities when it came to cyclist-friendly infrastructure.

Rider Dan Barnett, 27, said he sometimes struggled to feel safe on the roads.

"I'm just sick of being beeped at and yelled at, just feeling unsafe," the research assistant told AAP.

After briefly lying on the bridge, the protesting riders dinged their bells at bemused pedestrians as they were escorted by police on a mass bike ride through Brisbane's CBD.