Duke University will pay $112.5 million to the federal government to settle allegations that researchers submitted applications and reports containing falsified data to win more than two dozen grants from the National Institutes of Health and the Environmental Protection Agency, the Justice Department said on Monday.

“Taxpayers expect and deserve that federal grant dollars will be used efficiently and honestly,” Matthew G.T. Martin, United States attorney for the Middle District of North Carolina, said in a statement. “May this serve as a lesson that the use of false or fabricated data in grant applications or reports is completely unacceptable.”

The allegations were initially made in a whistle-blower lawsuit brought by Joseph Thomas, a research analyst who worked in Duke’s pulmonary division. He claimed that another researcher, Erin Potts-Kant, had fabricated data linked to as much as $200 million in federal research grants.

Mr. Thomas filed the lawsuit under the False Claims Act, a federal law that allows individuals to sue on behalf of the government. Under the law, the plaintiff may receive a portion of the damages. Mr. Thomas is to receive more than $33 million from the settlement.