The Italian rider was due to appear alongside Maverick Vinales, Dani Pedrosa, Andrea Dovizioso, Johann Zarco and Moto2 points leader Franco Morbidelli, but will have his place taken by Andrea Iannone.

Rossi is however still likely to appear in a brief media scrum after he arrives at the Mugello track on Thursday for a medical check-up following his motocross accident last week.

The 38-year-old spent the night in hospital in Rimini after sustaining chest and abdominal trauma in a crash at the Cross Club Cavallara last Thursday, but was discharged the following evening.

While Rossi is clearly eager to compete in his home race as normal, he admitted he still feels "severe" pain, which has preventing him from confirming with certainty that he will race.

After crashing out of the last race at Le Mans while fighting teammate Vinales for victory, Rossi now finds himself third in the championship, 23 points behind the Spaniard.