a, Lymphoma cells were isolated from Eμ-Myc mice and allowed to replicate in culture. Cells were injected subcutaneously (5 × 105 per flank) into nude mice and allowed to form tumours. Once tumours (Ctr, n = 4; −SG, n = 4) were visible and measurable, mice were transferred to control (Ctr) or SG-free diet (−SG). Mice were killed and tumours excised at single temporal end point (6 days on diet). Average tumour volume (as percentage of starting tumour volume) is shown, error bars are s.d. See Source Data. b, To assess cell number per subcutaneous Eμ-Myc tumour cross-section, two separate cell counts per tumour (using H&E-stained cross-sections) were performed and averaged, mean of means is shown, error bars are s.e.m. P values calculated by t-test (unpaired, one-tailed). c, Whole subcutaneous Eμ-Myc tumour tissue sections (Ctr, n = 3; −SG, n = 4) were immunostained for cleaved caspase-3 (CC3) and BrdU. Image analysis of non-necrotic regions of whole tumours allowed quantitative evaluation of the percentage of cleaved caspase-3-positive cells per tumour and the percentage of BrdU-positive cells per tumour. Data are averages, error bars are s.d. d, Eμ-Myc tumour (as described in a–c above) cross-sections were H&E stained, the scale bar for each image is 4 mm, demonstrative necrotic regions marked with arrows. Additional tumour tissue sections (marked with asterisks) are included for comparison from tumours, which developed after diet change (these three tumours were measurable 2 days post diet change and were present for 4 days on diet before end point). e, Necrosis was quantified by image analysis of necrotic and non-necrotic surface area of H&E stains for the sections shown in d. Error bars are s.d. P value was calculated by t-test (unpaired, one-tailed; Ctr, n = 5; −SG, n = 6). f, ApcMin/+ mice were placed on control diet (Ctr, n = 3) or SG-free diet (−SG, n = 3) at 80 days of age. At a single temporal end point (14 days on diet) mice were killed the small intestine was removed for histological analysis. Tissue sections were immunostained for cleaved caspase-3 and BrdU. Image analysis of whole intestines allowed quantitative evaluation of cell number per adenoma, percentage of CC3-positive cells and percentage of BrdU-positive cells per adenoma. Data are averages of all adenomas identified in each small intestine section, error bars are s.e.m., P values calculated by t-test (unpaired, one-tailed). For all analyses (a–f), P values below 0.1 are shown. Source data