The Islamic Jihad militant group said four of its members were killed in a “work accident” in the southern Gaza Strip along the border with Israel.

The explosion struck a three-wheeled vehicle near one of the five protest camps Palestinians have set up along the volatile Gaza-Israel border in recent weeks.

Islamic Jihad said all four were killed “during preparations” without elaborating, implying they were preparing an attack against Israel.

The Israeli military said it was unaware of any activity in the area of the blast.

It comes a day after another mass gathering along the border area in which thousands of Palestinians, some burning Israeli flags and torching tyres, staged a protest against the 10-year-old blockade. Israel has accused Hamas and other militant groups of using the protests as a cover for attacks and says its snipers have only targeted “main instigators”.

One protester was killed on Friday, bringing the death toll to 28 over the last two weeks, with more than 1,500 wounded by Israeli fire since 30 March.

The marches have been organised by Gaza’s militant Hamas rulers, but large turnouts on two preceding Fridays were also driven by Gaza’s dire living conditions and desperation among the territory’s two million residents who have been enduring a crippling border closure by Israel and Egypt since 2007.

Palestinians clash with Israeli troops during mass demonstrations Show all 10 1 /10 Palestinians clash with Israeli troops during mass demonstrations Palestinians clash with Israeli troops during mass demonstrations Palestinian protesters flee from teargas AFP/Getty Palestinians clash with Israeli troops during mass demonstrations Israeli soldiers stand as Palestinian protesters gather on the Israel Gaza border AP Palestinians clash with Israeli troops during mass demonstrations A Palestinian is carried on a stretcher after being injured during the demonstration AFP/Getty Palestinians clash with Israeli troops during mass demonstrations Israeli soldiers take aim as they lie prone over an earth barrier along the border with the Gaza strip in the southern Israeli kibbutz of Nahal Oz as Palestinians demonstrate on the other side commemorating Land Day AFP/Getty Palestinians clash with Israeli troops during mass demonstrations Palestinian paramedics evacuate an injured man on the Gaza side of the Israel-Gaza border Reuters Palestinians clash with Israeli troops during mass demonstrations Palestinians demonstrate with crossed-out posters depicting Israeli Prime Minister Benjamin Netanyahu and US President Donald Trump, during a tent city protest near Khan Yunis in the southern Gaza Strip AFP/Getty Images Palestinians clash with Israeli troops during mass demonstrations Palestinian protesters flee from teargas AFP/Getty Palestinians clash with Israeli troops during mass demonstrations Israeli soldiers shoot tear gas grenades towards the Palestinian tent city protest commemorating Land Day AFP/Getty Palestinians clash with Israeli troops during mass demonstrations Palestinian protesters take cover from Israeli troops AP Palestinians clash with Israeli troops during mass demonstrations Palestinians chant slogans as they attend a demonstration near the Gaza Strip border AP

Rights groups have described the Israeli military’s open-fire regulations as unlawful, saying they permit soldiers to use potentially lethal force against unarmed protesters.

Hamas leaders have sent mixed signals about whether they plan an eventual mass breach of the border fence. The protests are to culminate in a large rally on 15 May, the 70th anniversary of Israel’s creation.