An off-duty F.B.I. agent whose gun went off after he did a back handspring at a nightclub this month turned himself in on Tuesday to face an assault charge, the Denver district attorney’s office said.

The agent, Chase Bishop, 29, who faces one count of second-degree assault, had been dancing at a club in the early morning hours of June 2 when he performed the back handspring, according to video of the episode that was broadcast by Denver7, a local news station, and has circulated widely. A firearm that had been in a holster fell on the floor, and when Mr. Bishop reached for the gun it discharged, injuring another patron in the lower leg, according to the district attorney’s statement.

Beth McCann, the district attorney, said in the release that more charges could follow depending on the results of a blood alcohol content analysis.

“We are filing this charge now rather than waiting until the B.A.C. report is received, which we understand could take another week, because sufficient evidence has been presented to file it,” Ms. McCann said.