Demolishers have moved in to begin Queensland’s biggest infrastructure project, the $5.4 billion Cross River Rail.

The work to demolish the single-storey concrete Go Print building is set to be finished by January.

The company, Caylamax, last week took possession of the Go Print site at Woolloongabba, next to the Gabba stadium, and began the $330,000 demolition.

"This milestone for the new Woolloongabba underground station is a pivotal part of our early works program," Deputy Premier Jackie Trad said.

"Once the Go Print site has been cleared it will be the central staging location for massive tunnel boring machines that will dig Cross River Rail’s twin 5.9 kilometre tunnels, creating a new rail expressway for south-east Queensland," she said.