Two women have allegedly punched and bit a bus driver after being asked to pay for their fare during a trip in Dandenong in Melbourne's south-east.

The women boarded route 893 bus at Dandenong Plaza just before 3:00pm AEDT on November 29 and did not pay their fare.

Driver Faavae Tuiloma said that when he asked them to swipe their Myki cards as the bus reached Dandenong Railway Station, the pair attacked him.

He said they hurled abuse at him before punching, biting and spitting at him. They then got off the bus.

Mr Tuiloma, 44, said their reaction was unexpected.

"She just punched me in the chest, I say 'That's it back off' ... I was looking at the lady, all of a sudden she ripped my shirt off and I just defend myself," he told reporters.

"When I came home that day my wife was so (shocked) when she saw me."

The father of five said he had forgiven his attackers.

"I hope you learn something from this and I hope you won't do it again," he said.

Two women, aged 40 and 22 from Noble Park, are being interviewed by Dandenong police.

Senior Constable Charith Pathirana from Dandenong Police, labelled the attack "a low act".

"The bus driver here, he's just doing his job to support his family and he wants to go home in one piece, like everyone," he said.

"There's no compromise, you jump on a bus you've got to pay your fare."