Senate Judiciary Committee Chairman Lindsey Graham, R-S.C., clashed with Sen. Mazie Hirono, D-Hawaii, on Wednesday after she ripped Attorney General William Barr for his handling of the public rollout of special counsel Robert Mueller's federal Russia investigation findings.

"You have slandered this man from top to bottom. If you want more of this you're not going to get it," Graham told Hirono during Barr's appearance before the Senate Judiciary Committee.

"You certainly have your opinion, and I have mine," Hirono replied.

[Read more: Democrats attack Barr on obstruction]

Hirono had earlier laid out a case for why Barr "should never have been involved in supervising" Mueller's probe into Russia interference in the 2016 election and possible collusion with the Trump campaign. She culminated her statement with a call for the attorney general to step down from the top job at the Justice Department.

"Mr. Barr, now the American people know that you're no different than Rudy Giuliani or Kellyanne Conway or any of the other people who sacrificed their once-decent reputation for the grifter and liar who sits in the Oval Office," Hirono said. "Being attorney general of the United States is a sacred trust. You have betrayed that trust. America deserves better. You should resign."

Barr was asked to testify before the Senate panel to answer lawmakers' questions about the Mueller inquiry.