780,070 animals were used for research, testing, and education in the United States and Puerto Rico in 2018, according to a Jan. 2020 USDA report. That number includes 133,634 rabbits, 59,4019 dogs, and 18,619 cats. The total excludes animals not covered by the Animal Welfare Act (AWA), including cold-blooded animals (such as reptiles and most fish), farm animals used for food, and birds, rats, and mice specifically bred for use in research.



Massachusetts used 82,177 animals covered by the AWA for research in 2018, the most of any state, followed by California (68,337) and New Jersey (62,862); Wyoming used the fewest at 421. All data below were reported by the US Department of Agriculture (USDA) Animal and Plant Health Inspection Service (APHIS).