a, Five gene-edited plants were produced by treating seedlings with Wus2 & ipt, Wus2 & STM or Wus2 alone. The type and amount of editing were determined by Sanger sequencing and TIDE analysis (see Methods). The predicted editing percentage for each PDS gene is shown. b, The types of edits predicted by TIDE are shown as percentages of total editing. Any percentage below 5% was deemed below the limit of detection by TIDE. The first number in the column is indicative of the percentage of that edit in PDS1; the second number is indicative of editing in PDS2. Because of a deletion in 4-2, the sequence could not be properly aligned, and thus no percentages are given for PDS2. See the Supplementary Data Set for DNA sequences of all mutants recovered.