(A) Schematic representation of the mouse Camp locus with exons 2–4 flanked by LoxP sites. The inset gel image represents the genotyping result of WT (+/+), heterozygote (f/+), and homozygous mutant (f/f) mice by using primers flanking Camp as indicated.

(B) Campf/f (WT) or Campf/fPdgfra-cre (Campf/f;Pdgfra-cre) littermate mice (2 months of age) were infected intradermally (i.d.) with S. aureus, and Camp mRNA expression (ratio to housekeeping gene Tbp) in skin or spleen tissues was measured by qRT-PCR analyses (n = 3–5 per group).

(C) Measurement of bacterial CFU from the infection edge area of the skin from infected Campf/f or Campf/fPdgfra-cre mice (n = 6 per group).

(D) 3-week-old, 2-month-old, 1-year-old, or 2-year-old WT mice were infected i.d. with S. aureus, and bacteria CFU count was measured from the infection edge area (n = 4–5 per group).

(E) Cathelicidin (red) and COLIV (green) immunohistochemistry staining in control or S. aureus-infected skin (representative of n = 3 per group). Nuclei were stained with DAPI (blue). Scale bars, 100 μm.

(F) qRT-PCR of Camp or Spp1 expression in S. aureus-infected skin or control skin (ratio to Tbp) (n = 3–5 per group).

(G) Flow-cytometry plots for gating strategies for dermal PDGFRA+THY1hi adipocyte progenitors (representative of n = 3 per group).

(H) Flow-cytometry quantification of the geometric mean fluorescence intensity (gMFI) of Sca1 expression in THY1lo or THY1hi CD31−CD45−PDGFRA+ dFBs in control or S. aureus-infected skin (n = 3 per group).

(I) Flow-cytometry plots of THY1 and PDGFRA in CD31−CD45−PDGFRA+ FBs in skin dermis or eWAT (representative of n = 3 per group).

(J) Overlaid histogram of THY1 expression in CD31−CD45-PDGFRA+ FBs in skin dermis or eWAT (representative of n = 3 per group).

(K) Stacked bar graphs showing age-dependent changes of the percentage of THY1lo, THY1med, and THY1hi cells in CD31−CD45-PDGFRA+ FBs in skin dermis or eWAT (average of n = 3 per group).

(L) Age-dependent changes of the percentage of THY1lo–medPDGFRA+ fibroblasts, THY1hiPDGFRA+ fibroblasts, CD11B+F4/80+ macrophages, CD11B+Ly6G+ neutrophils, CD11C+F4/80− dendritic cells, CD45+TCRγδ+ T cells, and CD45+TCRαβ+ T cells in the total dermal cell population as indicated (average of n = 3 per group).