Six Nations Police say three men have surrendered to police and face first-degree murder charges after a fatal shooting on Wednesday afternoon.

Police responded at a 1st Line Road home just after 1 p.m and found three men suffering from gunshot wounds and a vehicle riddled with bullets. The men had travelled a short distance, with the actual shooting taking place at a different location nearby at Chiefswood Road and 1st Line.

Police said the shooting was "not a random act of violence," and was related to an incident Tuesday night.

Two of the victims were airlifted to Hamilton General Hospital, where 16-year-old Ashton VanEvery was pronounced dead as a result of his injuries Wednesday evening.

Another man remains in hospital in serious but stable condition, while the third was treated at the scene Wednesday afternoon and refused to go to hospital.

The area of Chiefswood Road between 2nd Line and Townline Road remained closed Wednesday evening for the investigation. OPP were also at the scene on the reserve, located south of Hamilton.

After a brief investigation and communication through family members, three men surrendered to police around 8 p.m.

Facing first-degree murder charges are:

Dwight Ronald Walton, 19.

Kenneth Paul Appleton, 24.

Irvin Allan Maracle, 56.