Three people have pleaded not guilty to charges relating to climbing on top of a train during climate change protests and have been remanded in custody until next month.

The two men and a woman appeared at Highbury Corner Magistrates’ Court on Thursday morning in relation to the incident during Extinction Rebellion (XR) protests at Canary Wharf station in London the previous day.

They have not been granted bail and will remain in custody until they are next due to appear at court on 16 May.

Those charged are Cathy Eastburn, 51, from Lambeth, south London, Mark Ovland, 35, of Somerton, Somerset, and Luke Watson, 29, of Manuden, Essex.

They each face charges of obstructing trains or carriages on the railway by an unlawful act contrary to Section 36 of the Malicious Damage Act 1861.

International climate protests enter fourth day: London sites occupied Show all 38 1 /38 International climate protests enter fourth day: London sites occupied International climate protests enter fourth day: London sites occupied Activists make some noise at the occupation of Oxford Circus in London Reuters International climate protests enter fourth day: London sites occupied A climate activist is arrested after spray painting a demand for climate laws on the entrance to Bristol Magistrates Court Bristol Live /SWNS International climate protests enter fourth day: London sites occupied Protesters in New York stage a "die-in" near City Hall in New York on April 17 Getty International climate protests enter fourth day: London sites occupied Jeremy Corbyn leaves his home as activists have glued themselves to the fence outside on April 17 SWNS International climate protests enter fourth day: London sites occupied An activist waters the plants at the occupation of Waterloo Bridge in London PA International climate protests enter fourth day: London sites occupied Activists sit in a boat on the River Thames in London on April 16 Reuters International climate protests enter fourth day: London sites occupied Two activists stand atop a DLR train while another has superglued his hand to the carriage in Canary Wharf DLR station in London Getty International climate protests enter fourth day: London sites occupied An activist sits in a hammock while occupying Oxford Circus in London AFP/Getty International climate protests enter fourth day: London sites occupied Protesters form a road block on the North Bridge in Edinburgh on April 16 PA International climate protests enter fourth day: London sites occupied Protesters dance as a DJ plays at Oxford Circus in London on April 17 Getty International climate protests enter fourth day: London sites occupied Police remove a protester from the roof a DLR train at Canary Wharf station in London AFP/Getty International climate protests enter fourth day: London sites occupied Protesters at the "Temple of Peace & Quiet" occupy Waterloo Bridge in London PA International climate protests enter fourth day: London sites occupied Police remove a protester from the roof a DLR train at Canary Wharf station in London AFP/Getty International climate protests enter fourth day: London sites occupied Protesters in New York stage a "die-in" near City Hall in New York Getty International climate protests enter fourth day: London sites occupied Protesters set up atop a lorry blocking Waterloo Bridge PA International climate protests enter fourth day: London sites occupied An activist walks past the camp on Waterloo Bridge early in the morning of April 17 in London Reuters International climate protests enter fourth day: London sites occupied Protesters on the Shell building in London during the protests PA International climate protests enter fourth day: London sites occupied An activist halts a train by superglueing his hand to it Getty International climate protests enter fourth day: London sites occupied Activists take part in a protest in Berlin on April 15 Getty International climate protests enter fourth day: London sites occupied Activists lie under a lorry on Waterloo Bridge in Central London during the protest PA International climate protests enter fourth day: London sites occupied A demonstrator is arrested on Waterloo Bridge during the second day of protests PA International climate protests enter fourth day: London sites occupied Activists carry a skeleton along the North Bridge in Edinburgh Getty International climate protests enter fourth day: London sites occupied Activists sit in the protests by Parliament Square in London EPA International climate protests enter fourth day: London sites occupied Protesters stand on Waterloo Bridge in Central London during the protests AFP/Getty International climate protests enter fourth day: London sites occupied Protesters carry giant wasp puppets in the protest by Parliament Square in London Getty International climate protests enter fourth day: London sites occupied A protester is arrested on Waterloo Bridge in Central London Reuters International climate protests enter fourth day: London sites occupied Activists take part in the second day of protests on Waterloo Bridge PA International climate protests enter fourth day: London sites occupied Activists wait under a float in the protest in Central London Reuters International climate protests enter fourth day: London sites occupied Waterloo Bridge is covered in graffiti after the protest in Central London Getty International climate protests enter fourth day: London sites occupied APRIL 15: Environmental campaigners block Oxford Circus during a coordinated protest by the Extinction Rebellion group on April 15, 2019 in London, England. With demonstrations blocking a number of locations across the capital, the group aims to stop traffic for up to five days Getty International climate protests enter fourth day: London sites occupied Demonstrators lay Ruby the rhinoceros during a Extinction Rebellion protest in Parliament Square in London. PRESS ASSOCIATION Photo. Picture date: Monday April 15, 2019. Activists have pledged to block five central locations in a non-violent act of resistance and rebellion that campaigners say could go on for weeks PA International climate protests enter fourth day: London sites occupied Environmental campaigners walk across Waterloo Bridge as they take part in a coordinated protest by the Extinction Rebellion group on April 15, 2019 in London, England. With demonstrations blocking a number of locations across the capital, the group aims to stop traffic for up to five days. Getty International climate protests enter fourth day: London sites occupied Environmental protesters from the Extinction Rebellion group stage a demonstration in Parliament Square London on April 15, 2019. - Environmental protesters from the Extinction Rebellion campaign group started a programme of demonstrations designed to block five of London's busiest and iconic locations to draw attention to what they see as the "Ecological and Climate Emergency" of climate change. AFP/Getty International climate protests enter fourth day: London sites occupied Environmental protesters from the Extinction Rebellion group stage a demonstration in Parliament Square London on April 15, 2019. - Environmental protesters from the Extinction Rebellion campaign group started a programme of demonstrations designed to block five of London's busiest and iconic locations to draw attention to what they see as the "Ecological and Climate Emergency" of climate change. AFP/Getty International climate protests enter fourth day: London sites occupied Demonstrators take part in a 'Funeral Procession' during a climate protest in Parliament Square in London, Monday, April 15, 2019. Extinction Rebellion have organised a nationwide week of action, they are calling for a full-scale Rebellion to demand decisive action from governments on climate change and ecological collapse. They plan to engage in acts of non-violent civil disobedience against governments in capital cities around the world. AP International climate protests enter fourth day: London sites occupied Protesters erect eco-friendly toilets to accompany the growing number of tents around Marble Arch. International climate protests enter fourth day: London sites occupied Demonstrators during a Extinction Rebellion protest in Parliament Square in London. PRESS ASSOCIATION Photo. Picture date: Monday April 15, 2019. Activists have pledged to block five central locations in a non-violent act of resistance and rebellion that campaigners say could go on for weeks. PA International climate protests enter fourth day: London sites occupied Demonstrators during a Extinction Rebellion protest in Oxford Circus in London PA

Dr Gail Bradbrook, a co-founder of Extinction Rebellion, has warned the protest group will escalate disruption if its demands “are not met” with “thousands more rebels” expected to join demonstrations over the Easter weekend.

It comes after some XR activists glued themselves to a train and others chained themselves to Labour leader Jeremy Corbyn’s garden fence on Wednesday after days of protests.

Scotland Yard said more than 100 people were arrested on Wednesday, bringing the total to almost 400 as ongoing protests continue in Parliament Square, Waterloo Bridge, Oxford Circus and Marble Arch.

Activists have said they plan to continue roadblocks, which have affected more than half a million people with traffic gridlock and disruption to transport and businesses since Monday, until at least next Friday.

Robin Boardman-Pattison, 21, who walked off during an interview with Sky News, said activists were planning to step up action on the rail and London Underground network.

“We will be escalating our disruption throughout the week,” he said. “The impact to the Tube system will grow.”

Asked about the protests on the BBC’s The One Show, environment secretary Michael Gove said the government had “got the message”.

He added: “I think it’s appropriate for people to make their feelings known but I also think we’ve got the message, we understand that action needs to be taken.”

XR activists, who are demanding a meeting with the government, say direct action is needed to force authorities to act urgently on climate change and wildlife declines and halt a “sixth mass extinction”.

TV presenter and naturalist Chris Packham joined protesters at Oxford Circus on Wednesday, saying: “I believe the world’s leaders are not acting urgently enough to avert a climate catastrophe.

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“As long as it is peaceful and democratic then they can count on my support.”

However a YouGov poll suggested public opinion has swung against the protests.

The survey of 3,561 British adults showed 52 per cent either “somewhat oppose” or “strongly oppose” the action, compared with 36 per cent who “strongly support” or “somewhat support” it.

Some Londoners were critical of the group’s methods, which are causing disruption to their lives.

Music student Anouska Stahlmann, 20, said her ill mother and elderly grandparents had to walk part of their journey because of the risk of getting stuck in a tunnel while on the Tube.

“I have no issue with wanting to better the environment and we’re fairly conscious of it as a family,” she said.

“Their methods, however, are seriously flawed and are not inclusive of people who want to support the cause.”