The world’s biggest publisher, Penguin Random House, has entered talks with Amazon that risk repeating the feud between the US online bookseller and French book publisher Hachette, which ended last year after a bitter six-month battle.

Penguin Random House, which publishes 15,000 books a year across 250 imprints, is the last of the “big five” publishers to renew its contract with Amazon on selling titles online. According to industry sources, talks are said to be ongoing, although neither side would divulge details.

Failure to reach a deal could result in Amazon freezing pre-orders or slowing down delivery of Penguin Random House titles, the hardball tactics that the online retailer deployed against Hachette last year. The bitter feud between Amazon and Hachette over the price of ebooks dragged on for more than six months and brought a wave of bad publicity for Amazon, with hundreds of bestselling writers accusing the online retailer of hurting authors and misleading customers. Hachette suffered a sharp drop in ebook sales before the dispute was resolved last November, although it said Amazon’s “punitive” tactics were only partly to blame.

Amazon’s spokesman, Tarek El-Hawary, declined to comment on rumours about its negotiations with Penguin Random House. “I can say that we have long-term deals in place already with the other four major publishers and we would accept any similar deal with Penguin Random House UK.” His remarks were first reported on recode.net.

Penguin Random House’s US spokeswoman, Claire von Schilling, sought to play down the negotiations. She said: “We are in continuous conversation with Amazon, with whom we have an ongoing business relationship. We have no intention whatsoever of ceasing to sell our print or digital titles on Amazon. We want our books to be accessible and available everywhere.” She denied there was any intention of ceasing to sell print or digital titles on Amazon.

A dispute would be costly for both sides, as Penguin Random House titles are a mainstay of Amazon’s bestseller lists. Some industry watchers think the world’s largest publisher has bolstered its negotiating position, following the merger of Penguin and Random House in 2013.

“With so many books in their repertoire comes tremendous negotiating power,” said Michael Kozlowski, the editor of goodereader.com. “There are not many online digital booksellers that could stay viable if suddenly all of the books published by Penguin Random House were pulled.”

According to the Bookseller magazine, the dispute centres on the agency deals that were agreed between Amazon and the UK arms of international publishing houses in the wake of a 2012 competition inquiry by the European Commission into ebook prices. Under the agency pricing model, which Amazon has been trying to unpick, publishers set the consumer price of ebooks, while retailers take commission.

Philip Jones, editor of the Bookseller, said it was helpful for the publisher that the dispute had flared up before the summer – well ahead of the peak pre-Christmas sales period. But he added: “I think we could be in for a prolonged period of public negotiation.” He said that Penguin Random House was in a “pretty good” position since the merger. “But Amazon – as we have seen from past experience – is not shy in pulling out the big guns.”

• This article was amended on 26 May 2015. An earlier version suggested that the publisher could block sales of its books on Amazon. This suggestion, added in the editing process, is theoretically-possible but gives undue weight to a remote possibility.