A total of 116 puppies were seized from two vehicles bound for the UK at Dublin Port on Wednesday.

Pugs, West Highland Terriers, Cocker Spaniels, Shih Tzus, Labroadoodles and Beagles were among the breeds being transported. All of the puppies were aged between four and eight weeks, the Dublin Society for the Prevention of Cruelty to Animals said.

Members of the DSPCA, the Department of Agriculture, Revenue and gardaí were involved in the operation.

None of the puppies seized had a Pet Passport, which is required under law to show the animal has been vaccinated, microchipped, wormed and seen by a vet.

A Garda spokesman said no arrests were made in connection with the matter.

A DSPCA spokeswoman said the puppies were inspected by vets on Thursday and were all being microchipped and vaccinated.

She said 32 of them were taken to veterinary hospital to be treated for diarrhoea and worms.

“The rest are in fairly good condition but are far too young to have been taken from their parents,” she said.

This is the first case under the Animal Health and Welfare Act 2013 which has involved a large number of companion animals being seized.

The DSPCA said the move was part of an operation targeting the illegal shipment of dogs that has been running for about three years. It is hoped that the seizure of the animals will lead to prosecutions.

The puppies will remain in foster care while the matter is being resolved, the spokeswoman said.