An 18-year-old man has been charged with manslaughter over the death of a man at Rye on Victoria's Mornington Peninsula.

David Cassai, 22, of Templestowe, died in hospital in Melbourne after he was struck and hit his head on the ground during a brawl at Rye early Monday morning.

Dylan John Closter, 18, of Millgrove, did not apply for bail at an out-of-sessions court hearing on Wednesday evening.

He will appear before a magistrate today.

A 24-year-old man from Kilsyth has also been charged with intentionally causing serious injury over the same brawl.

Earlier another two men - a 20-year-old man from Kilsyth and a 22-year-old from Yarra Junction - were questioned and released without charge.