Sadiq Khan is considering removing ‘ladies and gentlemen’ from announcements on Transport for London vehicles after a transgender woman was mocked by staff.

Aimee Challenor, 19, claims she was told she ‘didn’t sound like a miss’ during a call to the organisations over her Oyster card.

Miss Challenor, equalities spokesperson for the Green Party, has since called on Mr Khan to do more to ensure TfL improve their training around gender equality.

Aimee Challenor, pictured, said she was told she 'didn't sound like a miss' on a call to a Transport for London helpline

She told the Independent: ‘I knew it wasn’t right. London is massively diverse and it’s something we should be incredibly proud of so I knew it had to be raised, if it affected me it could affect anyone.’

Mr Khan has apologised for the incident and said while TfL does not offer trans specific training, he had asked for a review of its methods to ensure ‘staff put equality and fairness first’.

He also confirmed TfL had launched an investigation into the incident.

Speaking at a Question Time event last month in London, he said:

'TfL and I unreservedly apologise for the inappropriate remark made to a customer earlier this month.

'I have asked TfL to investigate this incident to ensure this does not happen again, as well as to apologise directly to your constituent.

'The lessons learnt here will be applied to all the functional bodies that make up the GLA family.

'TfL's approach is centred on treating everyone equally, while understanding the specific needs of different customers.

'Under this approach it does not offer trans-specific training, but rather ensures that all staff put equality and fairness first.

'I have asked for a review of training and procedures to ensure that TfL is always providing a professional, respectful service that is inclusive and sensitive to the needs of all Londoners.'

He added: 'TfL's LGBT+ staff network group, OUTbound, raises awareness of the needs of the LGBT+ community - customers and staff - at TfL.

'TfL also works with charity groups who represent the trans community, to foster understanding and encourage open dialogue to improve transport services.

'For example, it is working on implementing gender-neutral announcements across its network.'

Miss Challenor was represented at the Greater London Authority by Green Party member Sian Berry, who told the Independent many people would welcome the removal of ‘ladies and gentlemen’ from announcements.

Sadiq Khan, pictured, could potentially remove 'ladies and gentlemen' from TfL service announcements to promote gender equality

She said: ‘Gender neutral announcements will make a difference not just to trans people but to everyone who thinks being greeted as ‘ladies and gentleman’ is out of date.’

Ms Challenor told the paper she welcomed Mr Khan’s response but there was ‘more work to be done’.

It is understood the Mayor pointed to TfL’s work with LGBT charities during National Trans Awareness Week in November as one of the ways the organisation is dedicated to providing an equal service.

Mark Evers, Director of Customer Strategy at Transport for London, said: 'London is a diverse city and we want every customer to feel welcome on our network.