The federal government is being urged to revoke Royal Brunei Airlines' right to land in Australia after the Asian nation introduced the death penalty for homosexual acts and adultery.

The Victorian Gay and Lesbian Rights Lobby has called on Canberra to ensure the safety of Australians is protected.

Meanwhile, Virgin Australia on Wednesday confirmed it had severed its staff travel deal with the sultanate's national carrier over the new anti-gay laws.

Full sharia law, based on the Islamic religious and legal system, took effect in Brunei on Wednesday.

It applies to Muslims, non-Muslims and foreigners even when on Brunei-registered aircraft and vessels.

The Australian department of foreign affairs website advises would-be tourists of the new code, warning some offences may result in a penalty of execution.

Experts, however, are doubtful that executions will actually be carried out.

The Victorian lobby group believes the travel warning does not go far enough to protect the rights of LGBTIQ Australians.

"The penal code applies to all those who board a Brunei-registered aircraft even when in Australia," the VGLRL said.

"(It) will allow for inhumane and cruel punishments for queer people, such as death by stoning for consensual acts between same-sex adults."

The group also called on the only Australian airport with regular Royal Brunei flights - Melbourne Airport - to no longer accept flights from the carrier and for travel agents such as Flight Centre and STA Travel to immediately stop selling their flights.

Virgin Australia's move to immediately terminate the staff travel deal was announced to staff via email, which said the decision was made given the "harsh (including death) penalties being introduced for activity that is legal and acceptable in Australia".

A Virgin spokeswoman said a separate interline ticketing agreement that allows Royal Brunei to sell seats on Virgin flights had not been changed.

Virgin doesn't sell seats on the Brunei airline.

The revision of Brunei's penal code expands the crimes that can be punished by death to include rape, extramarital sex for Muslims, robbery, and insulting the Muslim prophet Mohammed.

Foreign Minister Marise Payne said Australia has raised concerns with the Brunei government over the new penal code.

"We absolutely oppose the death penalty and are committed to the rights of LGBTI people. We will continue to advocate for human rights in the region and beyond," she tweeted.

In a later statement, she added it was a matter for individual travellers to pick which airline they use.

Melbourne Airport chief executive Lyell Strambi said he found the new laws "extremely confronting" but says aviation access rights are a matter for the federal government and the airport would follow its response.

Travel agency Flight Centre said the company "vehemently opposes" the application of Sharia law in Brunei.