1. Taylor, R. C. & Dillin, A. Aging as an event of proteostasis collapse. Cold Spring Harb. Perspect. Biol. 3, a004440 (2011).

2. Krol, J., Loedige, I. & Filipowicz, W. The widespread regulation of microRNA biogenesis, function and decay. Nat. Rev. Genet. 11, 597–610 (2010).

3. de Lencastre, A. et al. MicroRNAs both promote and antagonize longevity in C. elegans. Curr. Biol. 20, 2159–2168 (2010).

4. Mori, M. A. et al. Role of microRNA processing in adipose tissue in stress defense and longevity. Cell Metab. 16, 336–347 (2012).

5. Segref, A., Torres, S. & Hoppe, T. A screenable in vivo assay to study proteostasis networks in Caenorhabditis elegans. Genetics 187, 1235–1240 (2011).

6. Denzel, M. S. et al. Hexosamine pathway metabolites enhance protein quality control and prolong life. Cell 156, 1167–1178 (2014).

7. Ruggiano, A., Foresti, O. & Carvalho, P. Quality control: ER-associated degradation: protein quality control and beyond. J. Cell Biol. 204, 869–879 (2014).

8. Vilchez, D. et al. RPN-6 determines C. elegans longevity under proteotoxic stress conditions. Nature 489, 263–268 (2012).

9. Calfon, M. et al. IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA. Nature 415, 92–96 (2002).

10. Boulias, K. & Horvitz, H. R. The C. elegans microRNA mir-71 acts in neurons to promote germline-mediated longevity through regulation of DAF-16/FOXO. Cell Metab. 15, 439–450 (2012).

11. Hsieh, Y.-W., Chang, C. & Chuang, C.-F. The microRNA mir-71 inhibits calcium signaling by targeting the TIR-1/Sarm1 adaptor protein to control stochastic L/R neuronal asymmetry in C. elegans. PLoS Genet. 8, e1002864 (2012).

12. Hobert, O. Terminal selectors of neuronal identity. Curr. Top. Dev. Biol. 116, 455–475 (2016).

13. Alcedo, J. & Kenyon, C. Regulation of C. elegans longevity by specific gustatory and olfactory neurons. Neuron 41, 45–55 (2004).

14. Troemel, E. R., Kimmel, B. E. & Bargmann, C. I. Reprogramming chemotaxis responses: sensory neurons define olfactory preferences in C. elegans. Cell 91, 161–169 (1997).

15. Jan, C. H., Friedman, R. C., Ruby, J. G. & Bartel, D. P. Formation, regulation and evolution of Caenorhabditis elegans 3′ UTRs. Nature 469, 97–101 (2011).

16. Chuang, C.-F. & Bargmann, C. I. A Toll–interleukin 1 repeat protein at the synapse specifies asymmetric odorant receptor expression via ASK1 MAPKKK signaling. Genes Dev. 19, 270–281 (2005).

17. Liberati, N. T. et al. Requirement for a conserved Toll/interleukin-1 resistance domain protein in the Caenorhabditis elegans immune response. Proc. Natl Acad. Sci. USA 101, 6593–6598 (2004).

18. Xie, Y., Moussaif, M., Choi, S., Xu, L. & Sze, J. Y. RFX transcription factor DAF-19 regulates 5-HT and innate immune responses to pathogenic bacteria in Caenorhabditis elegans. PLoS Genet. 9, e1003324 (2013).

19. Taylor, R. C. & Dillin, A. XBP-1 is a cell-nonautonomous regulator of stress resistance and longevity. Cell 153, 1435–1447 (2013).

20. Prahlad, V., Cornelius, T. & Morimoto, R. I. Regulation of the cellular heat shock response in Caenorhabditis elegans by thermosensory neurons. Science 320, 811–814 (2008).

21. Madison, J. M., Nurrish, S. & Kaplan, J. M. UNC-13 interaction with syntaxin is required for synaptic transmission. Curr. Biol. 15, 2236–2242 (2005).

22. Speese, S. et al. UNC-31 (CAPS) Is required for dense-core vesicle but not synaptic vesicle exocytosis in Caenorhabditis elegans. J. Neurosci. 27, 6150–6162 (2007).

23. Li, C. The ever-expanding neuropeptide gene families in the nematode Caenorhabditis elegans. Parasitology 131(Suppl.), S109–S127 (2005).

24. Chalasani, S. H. et al. Dissecting a circuit for olfactory behaviour in Caenorhabditis elegans. Nature 450, 63–70 (2007).

25. Hobert, O. et al. Regulation of interneuron function in the C. elegans thermoregulatory pathway by the ttx-3 LIM homeobox gene. Neuron 19, 345–357 (1997).

26. Bargmann, C. I. Chemosensation in C. elegans. in WormBook (ed. The C. elegans Research Community) https://doi.org/10.1895/wormbook.1.123.1 (2006).

27. Pokala, N., Liu, Q., Gordus, A. & Bargmann, C. I. Inducible and titratable silencing of Caenorhabditis elegans neurons in vivo with histamine-gated chloride channels. Proc. Natl Acad. Sci. USA 111, 2770–2775 (2014).

28. Ward, S. Chemotaxis by the nematode Caenorhabditis elegans: identification of attractants and analysis of the response by use of mutants. Proc. Natl Acad. Sci. USA 70, 817–821 (1973).

29. Ben Arous, J., Laffont, S. & Chatenay, D. Molecular and sensory basis of a food related two-state behavior in C. elegans. PLoS One 4, e7584 (2009).

30. Libert, S. et al. Regulation of Drosophila life span by olfaction and food-derived odors. Science 315, 1133–1137 (2007).

31. Maier, W., Adilov, B., Regenass, M. & Alcedo, J. A neuromedin U receptor acts with the sensory system to modulate food type-dependent effects on C. elegans lifespan. PLoS Biol. 8, e1000376 (2010).

32. Inoue, A. et al. Forgetting in C. elegans is accelerated by neuronal communication via the TIR-1/JNK-1 pathway. Cell Rep. 3, 808–819 (2013).

33. Essuman, K. et al. The SARM1 Toll/interleukin-1 receptor domain possesses intrinsic NAD+ cleavage activity that promotes pathological axonal degeneration. Neuron 93, 1334–1343 (2017).

34. Tsvetkov, P. et al. NADH binds and stabilizes the 26S proteasomes independent of ATP. J. Biol. Chem. 289, 11272–11281 (2014).

35. Summers, D. W., Gibson, D. A., DiAntonio, A. & Milbrandt, J. SARM1-specific motifs in the TIR domain enable NAD+ loss and regulate injury-induced SARM1 activation. Proc. Natl Acad. Sci. USA 113, E6271–E6280 (2016).

36. Pan, Z.-G. & An, X.-S. SARM1 deletion restrains NAFLD induced by high fat diet (HFD) through reducing inflammation, oxidative stress and lipid accumulation. Biochem. Biophys. Res. Commun. 498, 416–423 (2018).

37. Lin, C.-W. & Hsueh, Y.-P. Sarm1, a neuronal inflammatory regulator, controls social interaction, associative memory and cognitive flexibility in mice. Brain Behav. Immun. 37, 142–151 (2014).

38. Brooks, K. K., Liang, B. & Watts, J. L. The influence of bacterial diet on fat storage in C. elegans. PLoS One 4, e7545 (2009).

39. Riera, C. E. et al. The sense of smell impacts metabolic health and obesity. Cell Metab. 26, 198–211 (2017).

40. Teff, K. Nutritional implications of the cephalic-phase reflexes: endocrine responses. Appetite 34, 206–213 (2000).

41. Brenner, S. The genetics of Caenorhabditis elegans. Genetics 77, 71–94 (1974).

42. Stiernagle, T. Maintenance of C. elegans.in WormBook (ed. The C. elegans Research Community) https://doi.org/10.1895/wormbook.1.101.1 (2006).

43. Miedel, M. T. et al. A pro-cathepsin L mutant is a luminal substrate for endoplasmic-reticulum-associated degradation in C. elegans. PLoS One 7, e40145 (2012).

44. Roayaie, K., Crump, J. G., Sagasti, A. & Bargmann, C. I. The G α protein ODR-3 mediates olfactory and nociceptive function and controls cilium morphogenesis in C. elegans olfactory neurons. Neuron 20, 55–67 (1998).

45. Radman, I., Greiss, S. & Chin, J. W. Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection. PLoS One 8, e76019 (2013).

46. Drexel, T., Mahofsky, K., Latham, R., Zimmer, M. & Cochella, L. Neuron type-specific miRNA represses two broadly expressed genes to modulate an avoidance behavior in C. elegans. Genes Dev. 30, 2042–2047 (2016).

47. Friedland, A. E. et al. Heritable genome editing in C. elegans via a CRISPR–Cas9 system. Nat. Methods 10, 741–743 (2013).

48. Katic, I. & Großhans, H. Targeted heritable mutation and gene conversion by Cas9–CRISPR in Caenorhabditis elegans. Genetics 113, 155754 (2013).

49. Semple, J. I., Biondini, L. & Lehner, B. Generating transgenic nematodes by bombardment and antibiotic selection. Nat. Methods 9, 118–119 (2012).

50. Mitchell, D. H., Stiles, J. W., Santelli, J. & Sanadi, D. R. Synchronous growth and aging of Caenorhabditis elegans in the presence of fluorodeoxyuridine 1. J. Gerontol. 34, 28–36 (1979).

51. Timmons, L. & Fire, A. Specific interference by ingested dsRNA. Nature 395, 854 (1998).

52. Kamath, R. S., Martinez-Campos, M., Zipperlen, P., Fraser, A. G. & Ahringer, J. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2, RESEARCH0002 (2001).

53. Rual, J. F. et al. Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 14, 2162–2168 (2004).

54. Jadiya, P. & Nazir, A. A pre- and co-knockdown of RNAseT enzyme, Eri-1, enhances the efficiency of RNAi induced gene silencing in Caenorhabditis elegans. PLoS One 9, e87635 (2014).

55. Hoogewijs, D., Houthoofd, K., Matthijssens, F., Vandesompele, J. & Vanfleteren, J. R. Selection and validation of a set of reliable reference genes for quantitative sod gene expression analysis in C. elegans. BMC Mol. Biol. 9, 9 (2008).

56. Potluri, L. et al. Septal and lateral wall localization of PBP5, the major d,d-carboxypeptidase of Escherichia coli, requires substrate recognition and membrane attachment. Mol. Microbiol. 77, 300–323 (2010).