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A video of a bear urinating while performing at a circus in Council Bluffs last Saturday has led animal activists to call for the company to be investigated.

People for the Ethical Treatment of Animals posted the video of the bear, taken during a performance by the Tangier Shrine Circus at the Mid-America Center, urinating while walking on its front legs on a balancing beam.

“Based on eyewitness video footage of a bear urinating in apparent distress when pulled by a leash and forced to walk on his or her front legs,” the organization called for action.

The organization said the U.S. Department of Agriculture should investigate the act, known as “Castle’s Bears,” for perceived violations of the federal Animal Welfare Act.

In the video, the bear walks across the balance beam while being led by a circus performer, then down some steps before switching to its hind legs to sit in a chair.

In another video, a bear is led up the balance beam as the performer pulls on a chain connected to a muzzle.

The Tangier Shrine, based in Omaha, released a statement in the wake of the PETA video.