The House voted Tuesday night to block the Pentagon from using funding to implement its ban on most transgender troops.

The 243-183 vote came as an amendment to a $1 trillion spending package that includes the fiscal 2020 defense spending bill.

“The president and his administration wrongfully argue that it’s about military readiness and unit cohesion, but these arguments were the same ones that were used to keep the military racially segregated,” Rep. Anthony Brown Anthony Gregory BrownPelosi seeks to put pressure on GOP in COVID-19 relief battle Democrats demand Esper explicitly ban Confederate flag and allow Pride, Native Nations flags Trump tweets key GOP lawmaker has committed to not changing Confederate base names MORE (D-Md.) said on the House floor. “Transgender service members increase lethality, readiness. They have served honorably and received prestigious commendations.”

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The House previously voted in March to condemn the Trump administration’s transgender troops policy. At that time, five Republicans joined all Democrats in supporting the measure.

The Pentagon’s policy, which took effect in April, bans most transgender people from serving in the military unless they serve in their biological sex or were grandfathered in under the 2016 open-service policy.

The Trump administration and its allies deny the policy is a ban because of the carve-outs.

“This amendment risks undermining the readiness of our military at a time when we can least afford it,” Rep. Ken Calvert Kenneth (Ken) Stanton CalvertMORE (R-Calif.) said on the floor. “This current policy is not, I repeat, not a ban on service by transgender individuals. It carefully balances the readiness needs of the military with the medical needs of transgender individuals who wish to serve.”

Opponents argue the policy effectively is a ban akin to the defunct “don’t ask, don’t tell” policy that banned gay, lesbian and bisexual troops from serving openly.

Four lawsuits against the policy are still pending.

The March resolution against the policy was nonbinding, whereas Tuesday’s amendment would have an effect if it were to become law.

But the amendment is unlikely to become law. Still, LGBT advocates were tracking the House vote as sending a message.

"Military leaders don’t want this ban and the American people don’t want this ban -- including a growing percentage of the president’s own party," Shannon Minter, legal director of the National Center for Lesbian Rights, said in a statement Wednesday. "We won’t stop fighting in the courts to end the ban for good, and we applaud members of Congress for continuing to fight for our transgender service members as well.”

House Democrats are also expected to bring a transgender troops amendment to the floor when they debate the annual defense policy bill in the coming weeks.

Updated at 9:24 a.m. Wednesday