The Victorian Coalition's re-election hopes have been dealt another blow with the latest opinion poll suggesting a comfortable Labor win on November 29.

The Newspoll of more than 1,100 voters published in The Australian newspaper found Labor had a two-party preferred lead over the Coalition, of 54 to 46 per cent.

The Coalition's primary vote was 39 per cent, Labor 41 per cent, and the Greens 13 per cent.

The Coalition's primary vote has not climbed over 40 per cent in five surveys since August last year.

Denis Napthine was still the preferred premier, leading Daniel Andrews 47 to 34 per cent.

Thirty-six per cent of voters were satisfied with Mr Andrews' performance and 45 per cent were dissatisfied.

The Newspoll suggested Labor would be elected to power with a comfortable majority, making the Coalition the first single-term Government in Victoria since 1955.

The poll also showed that health and education were the two issues voters were most likely to rate as very important and influencing their vote.

Eighty-three per cent of voters rated health as very important, 78 per cent education and 70 per cent the economy.

The poll has a margin of error of about three per cent.