Affiliations

Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, 02139, Massachusetts, USA Johannes Gräff, Damien Rei, Ji-Song Guan, Wen-Yuan Wang, Jinsoo Seo, Martin Kahn, Susan C. Su, Alireza Samiei, Nadine Joseph & Li-Huei Tsai Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, 02139, Massachusetts, USA Johannes Gräff, Damien Rei, Ji-Song Guan, Wen-Yuan Wang, Jinsoo Seo, Susan C. Su, Nadine Joseph & Li-Huei Tsai Stanley Center for Psychiatric Research, Broad Institute of Harvard University and Massachusetts Institute of Technology, Cambridge, 02142, Massachusetts, USA Johannes Gräff, Ji-Song Guan, Wen-Yuan Wang, Krista M. Hennig, Thomas J. F. Nieland, Daniel M. Fass, Nadine Joseph, Stephen J. Haggarty & Li-Huei Tsai Center for Human Genetic Research, Massachusetts General Hospital, Harvard Medical School, Boston, 02114, Massachusetts, USA Krista M. Hennig, Daniel M. Fass & Stephen J. Haggarty Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston, 02118, Massachusetts, USA Patricia F. Kao & Ivana Delalle

Contributions

This study was designed by J.G. and L.-H.T., and directed and coordinated by L.-H.T. J.G. planned and performed the in vitro, CK-p25 and Cdk5cKO mouse and human in vivo biochemical characterization, and all behavioural experiments. D.R. planned and contributed to the in vitro and CK-p25 in vivo experiments, generated the GR526 and the shRNA constructs, and contributed to the stereotaxic injections. J.S.G. initiated and contributed to the CK-p25 biochemical characterization, and performed the 5XFAD and HDAC2−/− experiments. W.Y.W. generated the luciferase constructs. J.S. performed the electrophysiological experiments. K.M.H., T.J.F.N., D.F. and S.J.H. characterized the shRNA constructs. M.K. contributed to the quantitative RT–PCR experiments and performed the quantification of the human data. S.C.S. performed the site-directed mutagenesis. A.S. contributed to the immunohistochemistry and the quantitative reverse transcription/quantitative PCR experiments. N.J. contributed to the behavioural and quantitative reverse transcription/quantitative PCR experiments. P.F.K. and I.D. provided the human samples and contributed to the optimization of their staining. The manuscript was written by J.G. and L.-H.T. and commented on by all authors.

Corresponding author

Correspondence to Li-Huei Tsai.