Hundreds of Donald Trump fans have walked out of an Amy Schumer performance in Florida overnight after the comedian's show turned political.

Members of the audience booed and heckled Schumer as she turned to Donald Trump's history of sexual assault, calling him an "orange, sexual-assaulting, fake-college-starting monster", and targeted Republicans in the crowd.

According to the Tampa Bay Times, more than 200 members of the audience walked out of the show which became increasingly hostile as Schumer talked about her affiliation with Hillary Clinton and how Trump's behaviour had upset her personally as a victim of sexual assault.

The 35-year-old comedian threatened to kick out Republican audience members if they kept booing and interrupting the show, and told them to leave if they didn't like what she was saying.