Bacteria on leaves in tropical forests may play a vital role, protecting leaves against pathogens and even affecting the ability of forests to respond to climate change. Credit: Beth King, STRI

Human skin and gut microbes influence processes from digestion to disease resistance. Despite the fact that tropical forests are the most biodiverse terrestrial ecosystems on the planet, more is known about belly-button bacteria than bacteria on trees in the tropics. Smithsonian scientists and colleagues working on Panama's Barro Colorado Island discovered that small leaf samples from a single tree were home to more than 400 different kinds of bacteria. The combined sample from 57 tree species contained more than 7,000 different kinds.

Bacteria in tropical forests may also play a vital role, protecting leaves against pathogens and even affecting the ability of forests to respond to climate change.

"Just as people are realizing that microbes carried by humans can have an influence on a person's health—positive or negative—we hope to discover what bacteria on tree leaves can tell us about the health of a forest," said S. Joseph Wright, a Smithsonian scientist and co-author of the new study published in Proceedings of the National Academy of Sciences.

In contrast to a previous study of bacteria on leaves in a temperate forest where different leaves had different bacteria, there was a core group of species of common bacteria present on leaves of nearly all of the species sampled in Panama. Just as on human skin, many of the bacteria on tropical tree leaves were Actinobacteria and Proteobacteria.

The researchers, from the Smithsonian Tropical Research Institute, University of Quebec, University of Arizona, University of Oregon, University of California—Los Angeles and Santa Fe Institute, also looked for relationships between the composition of bacterial communities on leaves and other plant characteristics.

Many bacteria were associated with certain functional traits such as leaf thickness, wood density or leaf nitrogen content, characteristics that directly impact tree growth, survival and reproduction.

Smithsonian scientists and colleagues working in Panama discovered that small leaf samples from a single tree were home to more than 400 different kinds of bacteria. Credit: Beth King, STRI

The relationships between many of the bacteria and tree species they sampled were ancient, going back to the ancestors of both the bacteria and the trees as they evolved in tandem.

"Our ability to use molecular techniques like 16S ribosomal RNA gene sequencing to characterize nearly all of the bacteria on a leaf is going to make it possible to see how very different members of forest communities interact," said Wright. The Smithsonian forest ecologist and his colleagues hope to collaborate with researchers at the 60 Forest Global Earth Observatory sites coordinated by the Smithsonian's Center for Tropical Forest Science to compare interactions at different sites and under different environmental conditions.

Despite the fact that tropical forests are the most biodiverse terrestrial ecosystems on the planet, more is known about belly-button bacteria than bacteria on trees in the tropics. Credit: Beth King, STRI

More information: Kembel, S.W., O'Connor, T.K., Arnold, H.K., Hubbell, S.P., Wright, S.J. and Green, J.L. 2014 Relationships between phyllosphere bacterial communities and plant functional traits in a neotropical forest. Proc. Nat. Acad. Sci. early online edition: Journal information: Proceedings of the National Academy of Sciences Kembel, S.W., O'Connor, T.K., Arnold, H.K., Hubbell, S.P., Wright, S.J. and Green, J.L. 2014 Relationships between phyllosphere bacterial communities and plant functional traits in a neotropical forest.early online edition: www.pnas.org/cgi/doi/10.1073/pnas.1216057111