Strikingly, on that very same day Vine saw the spike, Instagram shares on Twitter surpassed Vine shares on Twitter, perhaps signaling that Vine users fled the platform to embrace the now-multi-purpose Instagram. (On May 30, shortly after Vine was released on Android devices, Vine was applauded when its shares on Twitter finally surpassed that of Instagram's.)

It's no secret that Facebook and Twitter—and more lately, respective subsidiaries Instagram and Vine—are competing for top dog in the social sharing world.

It's been well documented that the tech companies will not allow one another to feed off each other's data.

But shutting a rival out is a defensive move, and now, it appears that Facebook and Twitter are playing offense: Instagram moved quickly into Vine's videospace, so Vine, after hearing rumors this feature was coming, decided to tease new features, encourage Twitter users in an email to download Vine, and granted an informative interview to the New York Times. All, perhaps, in an effort to go all-in against its new rival Instagram.

Facebook and Twitter did not immediately respond for comment on the shift in social sharing.

Facebook investors, hungry for some good news, may take solace that there is now a strong indicator that the social media giant has the leg-up in the battle over social video.

What about monetization? In a recently released interview with Fast Company, Emily White, Instagram's new director of business operation, when pressed for a time frame for rolling out ad products on the currently non-monetized Instagram, said to expect a release "in the next year or so."

What has been interesting to watch is how Twitter executives have been quietly handling the Instagram news.

During Instagram's event, Twitter co-founder JackDorsey was thought to have subtweeted (defined as "tweeting at someone without mentioning their name) a quote to Instagram co-founder Kevin Systrom, who sold his app to Mark Zuckerberg instead of Dorsey.

Twitter CEO Dick Costolo can be heard discussing "Instagram" during a birthday party at Twitter headquarters in a vine shared by a Twitter employee on the day after Instagram's announcement.

It's quite evident that Twitter and Vine have Instagram on their mind, and that voice looks like it's only going to get louder.

Instagram changed its Twitter bio last week from "a fast, beautiful, and fun way to share your photos with friends and family" to "capturing and sharing the world's moments."

While Twitter has boasted that it is the "global townsquare," it's clear there may be a new sheriff headed into video town.

—Written by CNBC's EliLanger. Follow him on Twitter at @EliLanger