The death toll from a Melbourne nightclub shooting last weekend has risen to two after a second man died overnight after a week-long battle for his life.

Police confirmed a 28-year-old Maidstone man died in hospital on Friday night.

The man was lining up to get into the Love Machine nightclub in Prahran about 3.20am when he was shot.

Earlier, a security guard died after he was shot in the same incident. Two other men were taken to hospital in a serious condition.

A 26-year-old Meadow Heights man who presented himself to hospital with non-life threatening injuries believed to have been sustained at the shooting.

A 20-year-old woman was taken to hospital with non-life threatening injuries.

It is believed she may have fallen at the scene of the shooting and hit her head on the ground.

A 50-year-old Gisborne man and a 29-year-old Dallas man both sustained non-life threatening injuries.

Victoria Police Acting Commissioner Tess Walsh addressed the media as the police hunt for the gunman steps up.

She said the shooter remains at large but police are looking into whether the shooting involved Middle Eastern organised crime or outlaw motorcycle gangs.

Acting Commissioner Walsh said police are looking into a “range of different criminal entities”.

The shots were fired from a black Porsche Cayenne, which was later found burnt out in the suburb of Wollert, about 50km north of the crime scene.

Bloody clothes were found a short distance from the nightclub — which is reportedly a haven for strippers, bikies and other criminal types.

Allegations that bikies were extorting the venue is one of the police’s lines of inquiry, according to Detective Inspector Andrew Stamper.

News.com.au spoke with the family of a young woman who was at Love Machine and tried to help the injured.

The young woman was covered in blood and her family says she is traumatised by what she witnessed.

Another witness earlier recounted the terrifying moment a drive by shooter opened fire into a busy line outside the club.

Sandra, who witnessed the horrific shooting told The Age the club was still busy when the alleged shooter drove to the entrance and opened fire.

“I just saw the car roll up and then “pow, pow, pow, pow” — four times they shot,” she said.

“The people were all lining up to get in the club, they just start screaming and yelling “Oh my god, oh my god”.

“When the car left they ran to the people on the ground.”

NIGHTCLUB REACTS TO HORROR SHOOTING

After the horrific shooting in Melbourne nightclub — emotional tributes have been paid and thousands of dollars have been raised for a funeral.

Aaron Khalid Osmani from Narre Warren South was rushed to hospital early Sunday morning in a critical condition after the drive-by shooting and later died in hospital.

Now, his employer, the Love Machine nightclub, has released a statement saying it will be closed following the tragic news.

“The events that occurred outside the venue last night have shocked us deeply and we are working closely with authorities to assist with the ongoing investigation,” the statement read.

“We would like to take this opportunity to thank Victoria Police and Ambulance Victoria for their assistance, as well as our community for their ongoing support.

“Our thoughts are with the victims, their family and friends during this very difficult time.”

A Gofundme page has been set up to cover Mr Osmani’s funeral costs and it has raised more than $6000 so far.

It describes the doorman as “a kind, funny and easy going guy” and that it was “a great loss to anyone who has had a pleasure of knowing him”.

“Most people who have been to Love Machine or other establishments either know him personally or have met him,” it reads.

Those who knew Mr Osmani took to social media to pay tribute — with one of them saying he was “the most kind hearted soul I have met since I started working at Love Machine”.

“The most humble security guard I have ever had the pleasure to call a friend, my heart is broken. I love you bro,” a friend posted.

Another wrote: “You did not deserve to go like this” while another described the man as “the nicest guard I know.”

Love Machine host Steve Yousif posted on Facebook: “Overwhelmed with all your calls and texts, nothing but love for you all.

“What happened last night was uncalled for and devastating. For some of you it was a night out, the rest of the Love Machine family lost a beautiful soul today.

“No words, stay safe.”

Police confirmed four people had been transported to hospital following the shooting.

A 50-year-old man and a 29-year-old man remain in hospital with non-life threatening injuries.

Detective Inspector Andrew Stamper, from Victoria’s Homicide Squad, confirmed three of the people struck were employees and one person was a patron “innocently waiting to get in.

“Those gunshot wounds were from a fairly close proximity... so, you know... horrific injuries.”

Authorities are yet unclear if the shooting was a targeted attack, with Victoria Police Inspector Andrew Stamper telling media: “It is still an active crime scene. There is a lot of questions we don’t know at the moment. We are working through that.

“It would appear that shots have been discharged from a car in this area into a crowd that was standing outside the nightclub.

“This is just a horrendous act. It’s a busy nightclub, one of the main nightclubs in Melbourne in one of the main entertainment precincts in Melbourne.”

Stamper stressed that there was “nothing to indicate” that the shooting was part of a “broader agenda”.

Bloodstained clothing left in the middle of the road outside the club, alongside bullet casings seem to be the focus of detectives at the scene.

Forensic police have scoured the area swabbing and bagging any items of clothing they find in the area. Officers are photographing gutters and drains outside the nightclub as they continue searching for clues.

No arrests have been made and the gunman is still on the run.

Pat Mitchell, a police reporter for 3AW, tweeted that the footpath on Chapel St had been closed due to the trail of blood from the shot security guard.

One of the injured people ran to a nearby Chapel St business to seek help. Seven’s Sunday Sunrise spoke with a security guard at the club who said he had no idea there had been a shooting outside and there was no panic inside the venue, which was still operating.

A witness on social media said they are “in shock” after seeing a shooting “right in front of us...while we were standing across the road”.

“Please if anyone is out at clubs please go home now. We are all in shock but we are all okay.”

#BREAKING Multiple people, possibly three or four, shot outside Love Machine nightclub in Prahran this morning. One victim believed to have run a block onto Chapel Street, leaving a trail of blood. pic.twitter.com/KrpjSnJEaU — Paul Dowsley (@pauldowsley7) April 13, 2019

This is the scene outside Melbourne’s iconic Love Machine nightclub following a shooting early this morning. Multiple people have been injured. No official word on their conditions. @9NewsMelb @9NewsAUS pic.twitter.com/f2SgO1gEb8 — Sam Cucchiara (@SamCucchiara9) April 13, 2019

Prahran shooting update: two people in critical conditions in the Alfred Hospital, a third is in a stable condition. The footpath on Chapel St has just been closed due to trail of blood up to where one of the victims was found. @3AW693 pic.twitter.com/3JE7U6jQyQ — Pat Mitchell (@patty_mitchell) April 13, 2019

Multiple people shot outside a nightclub in Prahran. Details on Sky News @SkyNewsAust pic.twitter.com/zkHUi5OSn6 — Ahron Young (@AhronYoung) April 13, 2019

Sarah Guest, who lives a block away from the crime scene, said she isn’t shocked about the events given the mass drug use in the area.

“I’m not super surprised... walking to work one day I reckon I saw ten to 15 syringes in the gutter,” Ms Guest told The Age.

Edie, who lives in the nearby commission flats, told the Herald Sun: “There is a bit of trouble around here, but it’s usually fighting and stabbings.

“It’s scary because it’s right on your doorstep — it could have happened to one of us.”

Edie first heard about the shooting on the morning news and called on tougher gun laws.

“I think they should be stricter on the gun laws,” she told reporters on scene.

The shooting occurred just days after The Shooting Industry Foundation of Australia called for gun leadership from every candidate in Australia’s upcoming federal election.

Executive Officer Rod Drew says the time has come for every candidate to show commitment to Australian community safety and says the organisation’s aim is to get illegal guns out of their electorates.

“Australian voters care about safety, but the reality is that we have many thousands of illegal guns in the hands of criminals all over the country,” Mr Drew said.

“Australians deserve Members of Parliament and Senators who have a genuine, informed plan to get illegal guns out of our communities.”