Waste and Hygiene Compartment (WHC) Maintenance: Ground specialists met to discuss the WHC leak that occurred yesterday. Based on review of the imagery the crew took during the cleanup, the ground specialists determined the most likely source of the leak to be a hydroconnector on the Urine Valve Block, but determined that since all of the contaminated hardware would need to be replaced there was not a significant benefit to performing troubleshooting to isolate the root cause of the leak. Today, the crew replaced the Pump Separator, Urine Valve Block, several hoses and a data cable. The WHC was activated, checked out and is go for nominal operations.

Fluid Shifts Before, During and After Prolonged Space Flight and Their Association with Intracranial Pressure and Visual Impairment (Fluid Shifts): Today, a 48S crewmember began the first part of the Flight Day 45 (FD-45) Fluid Shifts data collection. Upon wakeup he collected baseline saliva, urine, and with another crewmember acting as operator collected blood samples that were inserted into Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) prior to ingesting a Sodium Bromide (NaBr) tracer. Throughout the day, the crew will perform more urine, blood, and saliva collections, inserting those samples into MELFI as well. Fluid Shifts investigates the causes for severe and lasting physical changes to astronaut’s eyes. Because the headward fluid shift is a hypothesized contributor to these changes, reversing this fluid shift with a lower body negative pressure device is investigated as a possible intervention. Results from this study may help to develop preventative measures against lasting changes in vision and eye damage.

Water Monitoring Suite (WMS) and Aquapad Sampling: In coordination with regular Environmental Health System (EHS) water sampling, the crew is scheduled to perform the ESA sponsored Aquapad (Paper Analytical Device) technological demonstration and sampling using the Microbial Monitoring System (MMS) portion of the WMS experiment. By using the same water collected for the EHS sample, ground teams can evaluate the capabilities of the new hardware. For the MMS, the crew reviewed an On-Board Training (OBT) video then configured the hardware to sample a low and high DNA concentration sample using the Razor Polymerase Chain Reaction (PCR) Microbial Monitoring System. The WMS is a set of hardware that monitors microbes, silica and organic material in the water supply on the ISS. The hardware ensures crew members can test and monitor the safety of their water supplies on future space missions, especially on long-duration missions to Mars, asteroids or other destinations where Earth-based testing would be difficult or impossible. Aquapad is a new approach developed by France’s CNES space agency: paper impregnated with powdered growth medium creates a 3D petri dish. When water is added, the microbes form colored spots revealing their locations. Using a tablet computer application the crew will photograph the dots to calculate precisely how many bacteria are present.

GeoFlow-2: The crew activated the Multi-purpose Computer and Communications (MPCC) laptop, Fluid Science Laboratory (FSL) rack, installed Anti Vibration Mount (AVM) brackets then released the Facility Core Element in preparation for free-floating microgravity science inside the FSL. Geoflow-2 studies heat and fluid flow currents within the Earth’s mantle. Geoflow-2 aims to improve computational methods that scientists and engineers use to understand and predict the processes in the Earth’s mantle that lead to volcanic eruptions, plate tectonics and earthquakes.

Spacecraft Fire Safety (Saffire)-II: Operations started onboard Cygnus about 2325 GMT yesterday and wrapped up about 0230 GMT when the post-test flow visualization completed. Preliminary telemetry indicated that data and images were recorded as expected for all 9 samples and flow visualization. The Saffire-II hardware performed nominally with no apparent issues. The team is downlinking the sensor and image data and begin analysis of the dataset later today.

Japanese Experiment Module (JEM) Stowage Wire Kit Installation: The crew started the activities to install the JEM Stowage Wire Kit. This Kit will make additional stowage available in the area under the JEM Airlock (JEMAL). The crew completed the activity to gather the hardware, but the rest of the activity was deferred due to work on the WHC.

Emergency Role/Responsibility Review: The crew performed a review of the Emergency General Instructions for response to emergencies and roles of each crew member. This review included a discussion of roles of Soyuz Commander, ISS Commander and each crewmember and strategies for working through the response to Emergencies.

Mobile Servicing System (MSS) Operations: The Robotics Ground Controllers translated the Mobile Transporter (MT) from Worksite (WS) 6 to WS7. Controllers will walk-off the Space Station Remote Manipulator System (SSRMS) from Mobile Base System (MBS) 3 to the MBS 1 grapple fixture. They will then unstow Special Purpose Dexterous Manipulator (SPDM). These activities are in preparation for Robotic External Leak Locator (RELL) operations planned to start Monday 28-November.

Today’s Planned Activities

All activities were completed unless otherwise noted.

Fluid Shifts Saliva Baseline Collection

Fluid Shifts Urine Collection

Fluid Shifts MELFI Insertion Operations

Fluid Shifts Blood Baseline Collection

Fluid Shifts Galley Water Collection Tracer Ingestion Operations

Fluid Shifts Refrigerated Centrifuge Configuration

KORREKTSIYA. Logging Liquid and Food (Medicine) Intake

Fluid Shifts USOS Operations Historical Documentation Photography

Virus Definition File Update on Auxiliary Computer System (ВКС) Laptops

FSL MPCC Laptop Hardware Relocation and Powering on Laptop

In Flight Maintenance Waste and Hygiene Compartment Stowage Gather.

Fluid Shifts MELFI Insertion

БД-2 R&R Ops.

Potable Water Dispenser (PWD) Sample Collect

FSL AVM brackets installation & Facility Core Element release in preparation of scientific operations

Fluid Shifts Urine Collection – Subject

WHC Urine Hydraulic Components R&R

Electrostatic Levitation Furnace(ELF) Argon gas QD connection

Total Organic Carbon Analyzer (TOCA) Potable Water Dispenser (PWD) Sample Analysis

Fluid Shifts MELFI Retrieve Insertion Operations

БМП Ф1 Absorption Cartridge Regeneration (start)

JEM Common Gas Support Equipment Gas Supply Start

Handover of Increment 49/50 Crew

JEM Stowage Wire Kit Installation Preparation

Fluid Shifts Blood 3-Hour Collection

Fluid Shifts Refrigerated Centrifuge Configuration

CEVIS Handover Video Review

ПТАБ-1М repositioning (from pos.А306 to pos.А308). Photography of de-installed ПТАБ-1М (А308)

CEVIS Crew Handover

Fluid Shifts MELFI Retrieve Insertion Operations

KORREKTSIYA. Logging Liquid and Food (Medicine) Intake

Fluid Shifts MELFI Retrieve Insertion Operations

Fluid Shifts Blood Collection Conclude Stow

WHC Pump Separator Control Unit Cable R&R

ARED Handover Video Review

ARED Crew Handover

WHC Pump Separator R&R

Deactivation of Soyuz 732 Gas Analyzer

Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record

Environmental Health System (EHS) Coliform Water Processing

Fluid Shifts Saliva 5-Hour Collection – Subject

Aquapad Double Water Sampling

In flight maintenance Waste and Hygiene Compartment closeout

Fluid Shifts MELFI Insertion Operations

Advanced Resistive Exercise Device (ARED) Cylinder Flywheel Evacuation

Fluid Shifts Urine Collection Stow

KORREKTSIYA. Logging Liquid and Food (Medicine) Intake

ПТАБ-1М Repositioning (from pos.А306 to pos.А308). Photography of de-installed ПТАБ-1М (А308)

High Definition Video Camera Assembly 1 Activation in High Definition Mode

KORREKTSIYA. Logging Liquid and Food (Medicine) Intake

In flight Maintenance WHC Stow

Portable Power Supply 1 Configuration for MagVector

Microbial Monitoring System On-Board Training Review

Data Transfer from MagVector to USB Jumpdrive

Environmental Health System (EHS) RAD Detector USB Cable USB Removal

Microbial Monitoring System Hardware Gather

VEG-03 Plant Photo

Microbial Monitoring System MWA Preparation

Health Maintenance System (HMS) Vision Test

Microbial Monitoring System Sample Setup

СОЖ Maintenance

Health Maintenance System (HMS) Vision Questionnaire

ESA Weekly crew conference

Columbus Video Camera Assembly 1 Adjustment

ISS Crew Adaptation

Robotic Workstation (RWS) Teardown

JEM Stowage Wire Kit Installation to JPM1D7 Part 1

Emergency Ammonia Measurement Kit Chip Measurement System (CMS) Swap

Microbial Monitoring System Sample

CONTENT. Experiment Ops

Emergency Roles and Responsibilities Review

Marrow Breath And Ambient Air Sample Setup – Subject

High Definition Video Camera Assembly 1 Switch to SD Mode

Microbial Monitoring System Data Transfer

Regeneration of БМП Ф1 Absorption Cartridge (end)

Completed Task List Items

None

Ground Activities

All activities were completed unless otherwise noted.

Lab MCA Zero Calibration

4B3 Battery reconditioning

MT Translation, SSRMS Walkoff to MBS1 and Unstow SPDM

Three-Day Look Ahead: (plans may change due to WHC R&R replanning)

Wednesday, 11/23: Everywear, JAXA Stowage outfitting, OBT Emer H/W Fam, Eye Exam, UIA Biocide Filter

Thursday, 11/24: Fluid Shifts, CBEF, PEPS Inspect, Storytime

Friday, 11/25: CIR, HRP Collections, ENERGY food repack, Eye Ultrasound, MARES EPM HD Install

QUICK ISS Status – Environmental Control Group: