Much local and regional transport policy is attempting to increase cycling as an everyday mode of travel through infrastructure changes, education initiatives, and safety campaigns. While considerable research has examined the influence of the built form on cycling, less research has examined the barriers that prevent people who wish to cycle more (as part of their routine) from doing so. This study examines several factors influencing the frequency by which people do (and do not) cycle in a campus setting in a large metropolitan area. Mixed methods reveal differences between barriers to cycling as well as the relative strength of these barriers across categories of age, sex, and current mode used. A multinomial logit model, which controls for residential self-selection effects, predicts whether and how often a respondent cycles based on socio-demographic and trip characteristics. The presence of cycle paths is found to be strongly associated with a higher frequency of cycling commutes. Additionally, an analysis of stated barriers reveals effort and a lack of safety as the most important barriers to potential cyclists. Finally, a qualitative analysis of respondents’ open-ended responses confirms the influence of bicycle paths, but reveals other factors such as the importance of improved interactions among various street users. Findings from this research can be of benefit to transportation engineers and planners who are aiming to increase the use of cycling among various groups of commuters.