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Pro golfer Tommy Gainey -- famously known as Tommy "Two Gloves" -- was arrested in a major prostitution sting in Florida ... TMZ Sports has learned.

The 44-year-old was 1 of 124 people busted in an undercover investigation in Polk County, Florida dubbed, "Operation Santa's Naughty List."

According to officials, Gainey was arrested for soliciting a prostitute -- and is facing a 1st-degree misdemeanor solicitation charge.

The sting op was a 6-day investigation using undercover detectives to target prostitution, human trafficking and child predators.

Cops say they used Internet advertisements to arrange meetings between the detectives and suspects.

Officials presented a "Naughty List" showing all 124 people arrested -- Gainey is on the board.

Jail records show Gainey was booked on Dec. 8 and was released on Dec. 9 after posting bail.

Sheriff Grady Judd explained the situation moments ago ...

Play video content Polk Co. Sheriff's Office

"He's married. He told us he was here for a charity golf event and it was supposed to be in the next morning tee off. He didn't make it. He was a scratch."

Judd added, "We charged him with soliciting. He missed his tee time the next morning."

Gainey has been a pro golfer since 1997 and joined the PGA Tour in 2008, where he gained notoriety for wearing gloves on both hands, unusual in the sport.

He's never won a major tourney but he's become a fan favorite for the way he dresses and plays -- in particular, he has a unique swing.

Gainey has had major sponsors over the years -- from Under Armour to Adams Golf and A.O. Smith.