The study is a collaboration between the University of Cambridge and Dutch company Hoekmine BV and shows how genetics can change the colour, and appearance, of certain types of bacteria. The results open up the possibility of harvesting these bacteria for the large-scale manufacturing of nanostructured materials: biodegradable, non-toxic paints could be 'grown' and not made, for example.

Flavobacterium is a type of bacteria that packs together in colonies that produce striking metallic colours, which come not from pigments, but from their internal structure, which reflects light at certain wavelengths. Scientists are still puzzled as to how these intricate structures are genetically engineered by nature, however.

"It is crucial to map the genes responsible for the structural colouration for further understanding of how nanostructures are engineered in nature," said first author Villads Egede Johansen, from Cambridge's Department of Chemistry. "This is the first systematic study of the genes underpinning structural colours -- not only in bacteria but in any living system."

The researchers compared the genetic information to optical properties and anatomy of wild-type and mutated bacterial colonies to understand how genes regulate the colour of the colony.

By genetically mutating the bacteria, the researchers changed their dimensions or their ability to move, which altered the geometry of the colonies. By changing the geometry, they changed the colour: they changed the original metallic green colour of the colony in the entire visible range from blue to red. They were also able to create duller colouration or make the colour disappear entirely.

"We mapped several genes with previously unknown functions and we correlated them to the colonies' self-organisational capacity and their colouration," said senior author Dr Colin Ingham, CEO of Hoekmine BV.

"From an applied perspective, this bacterial system allows us to achieve tuneable living photonic structures that can be reproduced in abundance, avoiding traditional nanofabrication methods," said co-senior author Dr Silvia Vignolini from the Cambridge's Department of Chemistry. "We see a potential in the use of such bacterial colonies as photonic pigments that can be readily optimised for changing colouration under external stimuli and that can interface with other living tissues, thereby adapting to variable environments. The future is open for biodegradable paints on our cars and walls -- simply by growing exactly the colour and appearance we want!"

Reference:

Villads Egede Johansen et al. 'Livingcolors: Genetic manipulation of structural color in bacterial colonies.' PNAS (2018). DOI: 10.1073/pnas.1716214115