A Queensland man accused of murdering his wife told police who were investigating her disappearance she dressed like a "hooker".

John William Chardon, 71, also told police that before she vanished in February 2013, his wife Novy had been acting strangely and he'd had to be careful what he told her because she "goes off".

Chardon told the officers he didn't know what had happened to his wife during that interview more than two weeks after the 34-year-old went missing amid a divorce dispute.

Novy Chardon was last seen on February 6, 2013, but her body has never been found despite an extensive search and a $250,000 reward. (AAP)

"I don't know a bloody thing... All I know is I didn't do anything to her," Chardon told police in the interview that was played on Tuesday to the jury in his Brisbane Supreme Court murder trial .

He told the officers Ms Chardon wore tight shorts "like a hooker" before she went missing.

Chardon also spoke of the divorce dispute during the long interview during which even his own lawyer reminded him to keep on track.

John Chardon is on trial for the alleged murder of his wife Novy five years ago. (AAP)

The millionaire lubricant businessman can be heard telling the officers of a $1200 week "allowance" he gave Ms Chardon and the $60,000 worth of handbags she had.

Under questioning from police he said he had been "shocked" to receive a letter from Ms Chardon's divorce lawyer at 3pm on the day she later disappeared - February 6, 2013.

He said he asked her about the letter later that night but she "totally ignored" him.

Chardon said he took a sleeping pill that night.

When he woke the next day, Ms Chardon was missing along with her car, laptop and other belongings, he said.

The trial continues on Wednesday.