Strict new rules to curb the dumping of yellow oBikes on Melbourne's streets are coming into force, with three councils cracking down on the share bike operator.

A one-year agreement has been signed by oBike Australia with Melbourne, Yarra and Port Phillip councils, with the company promising to clean up its act after dozens of the bikes were fished out of the Yarra River and found scattered along footpaths, and some even left up trees.

Central to the new agreement are tough new rules that stop oBikes from obstructing access to footpaths and fixed bicycle parking rails.

oBike must ensure its 1250 bikes on Melbourne's streets are: