Most insects, such as the Argentine ant seen here, are covered with a thin layer of hydrocarbon molecules as a waterproofing barrier. Credit: Mike Lewis, CISR, UC Riverside.

Most insects are covered with a thin layer of hydrocarbon molecules as a waterproofing barrier. Embedded in this layer are compounds that the insects use as chemical signals for a wide variety of functions such as communicating species and sex. In insects such as ants that live in colonies, they also differentiate the different castes (e.g., workers, queens, and drones).

But isolating these chemicals and determining their absolute configuration and functions has been a challenge because the chemicals occur in complex mixtures which are hard to separate.

Now a team of entomologists and chemists at the University of California, Riverside has devised a straightforward method for purifying these compounds that could result in new "green" methods of controlling pest species, like ants, by disrupting the organization of their colonies.

The researchers devised a technique that combined known fractionation methods with reverse phase high performance liquid chromatography - powerful tools in analysis. Specifically, they used their method to isolate 36 pure hydrocarbon molecules from the complex blends of 20 randomly chosen species in nine insect orders, so that these compounds could be conclusively identified, and the effects of the individual chemicals could be tested.

"In so-called social insects that live in large colonies, such as ants and bees, these chemicals have additional functions," explained Jocelyn G. Millar, a professor of entomology and chemistry, whose lab led the research team. "The queen in these colonies, for example, uses the chemicals to prevent her workers from laying eggs of their own, ensuring that she remains the only reproducing female in the colony."

The efforts of his research team were complicated by the fact that these chemicals can occur in right-handed (R) or left-handed (known as S, from sinistro, the Latin word for left) forms. Moreover, Millar and his colleagues did not know whether some insects produce the R form and others produce the S, or whether they all produced one form.

"This is critical information for biological activity, because if you have the wrong form, it is like trying to put your right hand into a left-hand glove," Millar said. "The wrong form of the chemical will simply not fit into its biological receptor."

His team was able to solve this problem by showing that all 20 insects that were tested had, regardless of species, sex and life stage, the R form of these chemicals.

"This suggests strongly that nearly all insects are likely to produce the R form of these chemicals," Millar said. "Knowing this will be of great help in unravelling what these signals do and how they work."

Study results appear online this week in the Proceedings of the National Academy of Sciences.

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