Washington lawmakers are taking aim again at the NFL over its response to domestic violence allegations against players — this time debating whether the league should be stripped of its permanent anti-trust exemption.

Sen. Richard Blumenthal (D-Conn.) is expected to take the lead at a hearing Thursday held by the Judiciary Committee. Blumenthal introduced a bill this week to end the “blank check” anti-trust exemption for America’s major sports leagues and instead make them seek a renewal every five years.

Only a league on its best behavior would see its exemption renewed after undergoing a congressional review. The hearing is expected to start at 10:15 a.m.

The anti-trust exemption enjoyed by the pro leagues for football, baseball, basketball and hockey is no small thing. Without it, the leagues may not be able to speak on behalf of all their teams when it comes to negotiating media deals, experts said.

Allowing the leagues to act as a monopoly has helped them reap billions of dollars each year, thanks mostly to lucrative broadcasting contracts, they said.

The NFL, feeling the heat since the Ray Rice domestic violence incident earlier this year, was a subject of a hearing on the problem in professional sports on Monday before the Senate Commerce Committee.

Rice, who was released by the Baltimore Ravens, was captured on video punching his then-fiancée unconscious in the elevator of an Atlantic City casino in February.

At that earlier hearing, NFL executive vice president Troy Vincent testified on his personal experiences with domestic violence while growing up.

While Vincent was praised for his honesty about witnessing abuse as a child, Democratic and Republican senators blasted the NFL for not sending Commissioner Roger Goodell.

Sen. Kelly Ayotte (R-NH) questioned whether Goodell was serious about tackling the domestic abuse issue if he didn’t bother to appear at the hearing.

“How big a commitment is there going to be on this?” Ayotte said.

On Thursday, Gerard Waldron, a partner at Covington & Burling, the NFL’s long-time outside lobbying counsel, is expected to testify.

Nita Chaudhary, co-founder of women’s advocacy group Ultraviolet, told The Post she expected some senators to broach the domestic violence issue again as well.

In laying out a new, tougher policy for NFL players found guilty of domestic violence, Goodell said there would be a six-game suspension without pay for the first offense and a lifetime ban for a second offense.

Goodell was criticized initially for giving Rice just a two-game ban in July following the Feb. 15 incident.

The video of Rice punching Janay Rice, who is now his wife, surfaced after he spent the first game of the season on the sidelines.

After the tape sparked national outrage, Goodell increased the penalty to an indefinite suspension and the Ravens cut Rice loose.

On Nov. 28, an arbitrator overturned the ban, calling Goodell’s decision “arbitrary.”