The man accused of driving a pickup truck down a crowded Manhattan bike path in what officials called the deadliest terrorist attack on New York City since Sept. 11, 2001, pleaded not guilty on Tuesday to multiple charges of murder and attempted murder in aid of racketeering.

The man, Sayfullo Saipov, was charged in Federal District Court in Manhattan with eight counts of murder in aid of racketeering activity, 12 counts of attempted murder in aid of racketeering activity, providing material support and resources to a foreign terrorist organization, and violence and destruction of motor vehicles.

The racketeering activity referred to the terrorism of the Islamic State, also known as ISIS; according to the indictment handed up by a grand jury on Nov. 21, Mr. Saipov acted “for the purpose of gaining entrance to ISIS.”

After plowing through bikers and pedestrians on the Hudson River Greenway shortly after 3 p.m. on Oct. 31, Mr. Saipov leapt out of his rented Home Depot truck and brandished a pellet gun and a paintball gun, shouting “Allahu akbar,” Arabic for “God is great,” before he was shot by a police officer, the authorities said.