The Victorian Ombudsman's office has asked the Supreme Court to rule whether it has the power to investigate allegations Labor MPs misused their parliamentary entitlements.

A number of Victorian Labor MPs have been accused of allowing paid parliamentary staffers to work in campaigning roles in the lead-up to the 2014 state election.

Last year, Victoria's Upper House referred the allegations to the ombudsman for investigation.

Ombudsman Deborah Glass has sought a declaration from the Supreme Court about whether the matter is in her jurisdiction.

"I have formed the view that there is genuine controversy as to my jurisdiction to investigate the matters in the parliamentary referral," Ms Glass said.

"In the absence of any precedent on the scope of section 16 of the act, to pre-empt any unnecessary cost being incurred, and given my role as an independent officer of the Parliament, I believe the present application is the most appropriate way to determine the matter."

Parliamentary rules state that employees paid by the Victorian Parliament should not be involved in party political campaigning.

The Labor Party has denied any wrongdoing and maintained staff were part of a "pooling" arrangement that allowed them to move between different roles and they were not campaigning.

Premier Daniel Andrews said it was the ombudsman's right to seek the court's guidance.

"I'm respectful of the fact that the ombudsman has the opportunity to seek clarification as to her powers and I wouldn't want to cut across that process," he said.

Opposition Leader Matthew Guy said the Government was trying to shut down the investigation.

"The ombudsman has the right to investigate the operating budget of a member of parliament, and I would have thought that falls entirely within the scope of what the Legislative Council has asked the ombudsman to investigate," he said.

"Therefore to me it just stinks that the Andrews Government is leaving no stone unturned to try to scuttle the ombudsman doing her job on behalf of all Victorians."