Two University of Cincinnati police officers who were present when a colleague shot and killed a motorist were placed on paid leave, a university spokesman said Thursday, while a judge set bail of $1 million for the officer at the center of the case.

That officer, Ray Tensing, appeared briefly in a Hamilton County court in Cincinnati, in gray-and-black striped jail garb, handcuffed behind his back, as his lawyer, William S. Mathews II, entered a plea of not guilty for him. Mr. Tensing, who has been fired by the university, was released from jail on bond later Thursday.

On Wednesday, county prosecutors announced that a grand jury had indicted Mr. Tensing on charges of murder and manslaughter in the killing of Samuel DuBose on July 19 during a traffic stop for a missing license plate. They said it was the first time a law enforcement officer in the county had ever faced a murder charge for actions taken in the line of duty.

The prosecutors also released a graphic and widely anticipated video of the shooting, taken by a body camera worn by the officer, which they said was crucial to the case, and showed that Mr. Tensing and possibly other officers had misrepresented what had happened.