Beyoncé surprised her fans with an appearance at tonight's Country Music Awards that was announced just a few hours before showtime.

For the ninth consecutive year, Carrie Underwood and Brad Paisley co-hosted the evening, and by the third hour of Wednesday night's ceremony, Queen Bey hit the stage to perform "Daddy Lessons" off her latest album, Lemonade.

It was the superstar's first performance at the awards show and she showed up with surprise guests, the Dixie Chicks, who made their first appearance in more than a decade.

Beyoncé performs at the Country Music Awards Show all 5 1 /5 Beyoncé performs at the Country Music Awards Beyoncé performs at the Country Music Awards Beyonce performs onstage with Martie Maguire of Dixie Chicks at the 50th annual CMA Awards at the Bridgestone Arena on November 2, 2016 in Nashville, Tennessee. Rick Diamond/Getty Beyoncé performs at the Country Music Awards Beyonce (R) performs onstage with Emily Robison and Natalie Maines of Dixie Chicks at the 50th annual CMA Awards at the Bridgestone Arena on November 2, 2016 in Nashville, Tennessee. Rick Diamond/Getty Beyoncé performs at the Country Music Awards Beyonce performs onstage with Martie Maguire of Dixie Chicks at the 50th annual CMA Awards at the Bridgestone Arena on November 2, 2016 in Nashville, Tennessee. Rick Diamond/Getty Beyoncé performs at the Country Music Awards Beyonce (2nd R) performs onstage with Emily Robison, Natalie Maines, and Martie Maguire of Dixie Chicks at the 50th annual CMA Awards at the Bridgestone Arena on November 2, 2016 in Nashville, Tennessee. (Photo by Rick Diamond/Getty Images) Rick Diamond/Getty Beyoncé performs at the Country Music Awards Beyonce (2nd R) performs onstage with Emily Robison, Natalie Maines, and Martie Maguire of Dixie Chicks at the 50th annual CMA Awards at the Bridgestone Arena on November 2, 2016 in Nashville, Tennessee. Rick Diamond/Getty

Shortly after the performance, both acts released studio quality version of the single online.

Last year, Memphis-born pop star Justin Timberlake joined the CMAs for a performance with country singer Chris Stapleton.

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"Happy 50th anniversary, CMA," Beyonce said at the end of the set as she hugged Natalie Maines, Martie Maguire, and Emily Robison.

You can listen to Beyoncé's "Daddy Lessons" featuring the Dixie Chicks on Soundcloud. And watch the performance at the CMAs below.