Jocelyn Ahlf ... Lanteia / Nyxeris / Synthetic Insights Rep / Afterlife Patron / Asari Police / Blue Suns Enemies / Cerberus Announcement / 'Citadel' Advertisement Shepard / Spaceport Official / Survivor (voice)

April Banigan ... Elnora / Khalisah Bint Sinan al-Jilani / Seryna / Afterlife Patron / Asari Commando / Clinic Receptionist / Closure Announcement / Eclipse Lieutenant / Eclipse Vanguard / Elevator Door / Ensign / LOKI Mechs / Refugee (voice)

Ashley Barlow ... Afterlife Patron / C-Sec Officer / Colonial Administration Advertisement / Eclipse Vanguard / Franklin Anomaly / Harrot's Advertisement / Sinmara Anomaly / Transport Officer (voice) (as Ashley Jill Barlow)

Brian Bloom ... Charr / Ratch / Mad Prophet / M-44 Hammerhead VI - Firewalker DLC / Keiji Okuda - Kasumi: Stolen Memory DLC / Party Guest - Kasumi: Stolen Memory DLC / Cerberus Researcher - Overlord DLC / Lab Technician - Overlord DLC (voice)

Jessica Anne Bogart ... Tela Vasir - Lair of the Shadow Broker DLC / Quarian Researcher / Ship Computer (voice)

Wendy Braun ... Gianna Parasini / Asari Bar Owner / Arguing Asari / Asari Advertisement / JenanTech Advertisement / Sophist Advertisement / MSV Hugo Gernsback Survivor (voice)

Kimberly Brooks ... Operations Chief Ashley Williams (voice)

Lora Brovold ... Careena / Hala'Dama / Galactic Newscaster / Computer / Eclipse Enemies / Gei Hinnom Anomaly / Survivor / Alarm Clock - Kasumi: Stolen Memory DLC / Eclipse Trooper - Kasumi: Stolen Memory DLC / Party Guest - Kasumi: Stolen Memory DLC / Vault Computer - Kasumi: Stolen Memory DLC / Announcements - Overlord DLC / Announcements - Lair of the Shadow Broker DLC / Injured Civilian - Lair of the Shadow Broker DLC / Operative Shora - Lair of the Shadow Broker DLC (voice)

Belinda Cornish ... Rana Thanoptis / MSV Hugo Gernsback Doctor (voice)

Jim Cummings ... Patriarch / Urdnot Wreav / Chief Weyrloc Guld / Captain Kratt / Crewman Rolston / Dah'tan Advertisement / Devlon Advertisement / Prophet Advertisement / Scientist (voice)

Jon Curry ... Tank-Grown Krogan / Wounded Merc / Bachelor Party Human / Blue Suns Driver / Blue Suns Trooper / Burgat Advertisement / Chef / Clinic Guard / Dark Star Advertisement / Krogan Bodyguard / Prison Guard / Turian Bystander (voice)

Josh Dean ... Horftin / Jonn Whitson / Clinic Receptionist (voice)

Casey DeFranco ... Illium Newscaster / Illium Advertisements / MSV Hugo Gernsback Survivor (voice) (as Casey Defranco)

Grey Griffin ... Nassana Dantius (voice) (as Grey DeLisle)

Chris Edgerly ... Etarn Tiron / Sel / Bachelor Party Turian / Investment Advertisement / Prison Guard / Salarian Bartender / Technician (voice)

Jeannie Elias ... Crewman Patel / Survivor (voice)

Dave Fennoy ... Warlord Okeer / Ronald Taylor / Tarak / Turian Commuter (voice) (as Dave Fenoy)

Keith Ferguson ... Niket / Garka / Merc Leader / 'Citadel' Advertisement Shepard / Omega Newscaster / Ruthoss Jenera Advertisement / Survivor (voice)

Peter Giles ... Garm / Kargesh / Moklan / Salkie / Batarian Patient / Chief Scout / Fishdog Advertisement / Krogan Mechanic / Nalinox Advertisement (voice)

Zach Hanks ... Florit / Afterlife Patron / Blue Suns Enemies / Blue Suns Trooper / General Distress Signal / Navigator's Log (voice)

Martin Jarvis ... Admiral Zaal'Koris vas Qwib Qwib (voice)

Phil LaMarr ... Mouse / Rukar / Brainwashed Guards (voice)

Lex Lang ... Keystone / Elite Krogan Enemies / Stocks Volus / Urdnot Warrior / Used Ship Salesman (voice)

Yuri Lowenthal ... Daniel / Prisoner 780 / Cerberus Researcher / Eclipse Enemies / Refugee / Stock Boy / Technician / Feron - Lair of the Shadow Broker DLC / Sekat - Lair of the Shadow Broker DLC (voice)

Stefan Marks ... Maelon / Huck / Vertin / Blue Suns Legionnaire / District Guard / Turian Worker (voice)

Anndi McAfee ... Emily Wong / Hallia / Hermia / Quarian Slave (voice)

Cara Pifko ... Yeoman Kelly Chambers / Lia'Vael / Idenna Quarian (voice)

Chris Postle ... Pitne For / Eclipse Trooper / Human Looter / Turian Patient / Dr. Manuel Cayce - Firewalker DLC (voice)

Francesco Quinn ... Henner / Marsh / Batarian Log / Blood Pack Recruiter (voice)

John Rubinow ... Blue Suns Enemies / Turian Patron (voice)

Raphael Sbarge ... Staff Commander Kaidan Alenko (voice)

Dwight Schultz ... Jaroth / Navigator Pressly (voice)

Jane Singer ... Erinya / Helena Blake / Slave Broker / Burial Advertisement / Sad Asari (voice)

Jason Singer ... Sergeant Cathka / Sidonis / Batarian Victim / Batarian / Human Looter / Comm System - Arrival DLC / Prison Guard - Arrival DLC / Project Base - Arrival DLC / Project Guard - Arrival DLC (voice)

Jan Alexandra Smith ... Asari Councilor / Dr. Elan'Shiya / Officer Dara / Rebekah / Afterlife Patron / Arguing Asari / Asari Customer / Asari Shopkeep / Quarian (voice)

George Szilagyi ... Ish / Telon / Salarian Worker / Batarian / Cerberus Scientist / Eclipse Trooper / Eclipse Vanguard / Turian Bouncer (voice)

Fred Tatasciore ... Warden Kuril / Krogan Shaman / Weyrloc Clanspeaker / Polite Krogan (voice)

Mick Wingert ... Gunnery Chief / Activated Beacon VI / Blue Suns Trooper / Brainwashed Guards / Cerberus Scientist / 'Citadel' Advertisement Human / Eclipse Enemies / Emergency Shutter Control / Security Control (voice)

Stephanie Wolfe ... Jedore / Jinn / Bekke Burlesque Advertisement / Project Enemies - Arrival DLC / Scientist - Arrival DLC (voice)

John Wright ... Edwin / Fist / Ferlit Spokesman / Merc Recruiter / Quarian Scientist / Survivor / Turian Log / Urdnot Warrior (voice)

Gwendoline Yeo ... Shiala / Captain Wasea / Kian Louros / Quarian Patron (voice)

Frederick Zbryski ... Annoyed Human / Daratar Anomaly / Helyme Anomaly / Human Customer / Quarian / Salarian Customer / Turian Customer / Warehouse Salarian (voice)

Richard Green ... Vido Santiago - Zaeed: The Price of Revenge DLC (voice)

Stelio Savante ... Eclipse Security Guard - Kasumi: Stolen Memory DLC / Party Guest - Kasumi: Stolen Memory DLC (voice)

Clinton Carew ... Injured Civilian - Lair of the Shadow Broker DLC / Shadow Broker Enemies - Lair of the Shadow Broker DLC / Trade Center Advertisements - Lair of the Shadow Broker DLC (voice)

Patricia Zentilli ... Mariana - Lair of the Shadow Broker DLC / Civilian - Lair of the Shadow Broker DLC / Illium Cop - Lair of the Shadow Broker DLC (voice)

Lance Henriksen ... Admiral Steven Hackett - Arrival DLC (voice)

Victoria Gay ... Dr. Amanda Kenson - Arrival DLC (voice)