KAPA HyperPrep Kits offer a streamlined library preparation protocol, combining several enzymatic steps and eliminating bead cleanups to reduce library preparation time and improve consistency. The novel, single-tube chemistry offers further improvements to library construction efficiency, particularly for challenging samples such as FFPE and cell-free DNA. KAPA HyperPrep Kits offer complete library preparation solutions with KAPA Adapters and KAPA Pure Beads.

KAPA HyperPlus Kits, our most advanced library preparation option, provide a streamlined workflow that includes fully automatable fragmentation and library preparation in a single tube. This integrated solution combines enzymatic fragmentation, with the quality of mechanical shearing, and the speed and convenience of tagmentation-based workflows. KAPA HyperPlus Kitsoffer a complete library preparation solution with KAPA Adapters and KAPA Pure Beads. The kits are compatible with the Illumina sequencing platform and provide qualified automation methods.

The KAPA RNA HyperPrep Kits utilize a novel chemistry to enable the combination of enzymatic steps and fewer reaction purifications. This results in a truly streamlined solution for the preparation of high-quality RNA-seq libraries. The strand-specific workflow is flexible, supporting library construction from lower-input amounts and degraded samples. It is compatible with mRNA capture and ribosomal depletion. KAPA RNA HyperPrep Kits contain all reagents required for RNA library preparation, with the exception of KAPA Adapters (available separately).

KAPA Library Amplification Kits include KAPA HiFi DNA Polymerase, a novel enzyme engineered using our directed evolution technology for ultra-high fidelity and robustness. KAPA HiFi has become the enzyme of choice for NGS library amplification due to its ability to amplify complex DNA populations with high fidelity, high efficiency and very low amplification bias. This results in lower duplication rates and improved coverage of GC- and AT-rich regions, promoters, low-complexity and other challenging regions.

KAPA HiFi Uracil+ DNA Polymerase is a modified version of KAPA HiFi DNA Polymerase. It is engineered to tolerate uracil residues in bisulfite-treated DNA. Bisulfite treatment of DNA results in the conversion of unmethylated cytosine residues into uracil, while the methylated residues are left unmodified. KAPA HiFi Uracil+ DNA Polymerase has been developed to read through uracil residues while still retaining the performance benefits of HiFi DNA Polymerase, such as high yields, low-bias, and uniform sequencing coverage