Gold Logie winning actor Craig McLachlan has filed defamation proceedings against Fairfax Media and the ABC after they reported on allegations he sexually harassed several former colleagues during the 2014 production of the Rocky Horror Show.

One of McLachlan's accusers, former co-star Christie Whelan Browne, is also named in the defamation suit, according to media reports.

The Seven Network says McLachlan's statement of claim was lodged in the NSW Supreme Court on Thursday afternoon and principally targets Browne.

The claim states Browne was 'herself a notoriously foul-mouthed person who publicly distributed offensive matter and had expressed interest in deviant sexual practices,' Seven News reported.

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Craig McLachlan (right) is suing Christie Whelan (left) for defamation after she publicly accused him of sexually inappropriate behaviour

McLachlan is accused by three women of sexual assault and bullying during the 2014 run of the Rocky Horror Picture Show

McLachlan alleges that in addition to backstage behaviour, Whelan Browne's social media accounts contain multiple sexual references

McLachlan has engaged prominent barrister Stuart Littlemore QC to run the case, News Corp Australia reports.

The documents submitted to the court allege in addition to backstage behaviour, Whelan Browne uses social media to post material with sexual references.

A Fairfax Media and ABC joint investigation in early January reported several cast members of the 2014 run of The Rocky Horror Picture Show accused 52-year-old McLachlan of touching them or exposing himself to them.

A woman and a man who worked as part of the crew have also gone to Victorian police who are investigating the allegations.

McLachlan strenuously denies the claims and has previously described the allegations of bullying and sexual assault by Whelan Browne, Erika Heynatz and Angela Scundi as 'baseless', insisting they were 'all made up'.

He also vowed to fight the allegations.

'By God, I will fight this,' he told News Corp Australia.

Browne said that during Rocky Horror in 2014, McLachlan, who played transvestite Frank N Furter to her character Janet, indecently assaulted her on stage during a sex scene.

McLachlan's case will be heard on March 16 at the Supreme Court in Sydney

Ms Whelan is one of three women who have come forward alleging McLachlan's behaviour in the 2014 production of The Rocky Horror Picture Show was inappropriate

After the allegations were aired McLachlan withdrew from the current production of the Rocky Horror Show in Adelaide.

McLachlan, meanwhile, has been cleared of sexual harassment claims made by some colleagues on the set of popular TV series the Doctor Blake Mysteries.

Cast and crew reportedly made complaints about the actor's on-set behaviour which allegedly included holding a banana to his crotch and thrusting it into the faces of cast and crew, according to the ABC and Fairfax.

But an investigation by workplace consultant Fiona Bigelli, who was hired by the show's production company December Media, has cleared McLachlan of sexual harassment, sexual misconduct and workplace bullying claims.

McLachlan previously described the allegations of bullying and sexual assault by Whelan (pictured) Browne, Erika Heynatz and Angela Scundi as 'baseless', insisting they were 'all made up'

However, Ms Bigelli found many of the show's cast and crew had described the workplace culture and humour on the Doctor Blake set as 'Benny-Hill- esque' for its sexual, lewd, bawdy and crude humour.

'Some of the behaviour relating to this humour may be offensive to people regardless of the fact no formal complaints have been received,' December Media said in a statement.

Fairfax Media and the ABC on Thursday told AAP they wouldn't be commenting on the legal action.

Mr Littlemore said he would 'absolutely not' be commenting.

Comment has been sought from Mollison Keightley Management which represents McLachlan.