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As the first protest broke out in Jalalabad city over the weekend shootings, the Taliban said Afghan government demands for an open trial of the U.S. Army staff sergeant being held for the slayings would not blunt civilian hostility towards Western combat troops.

The unnamed U.S. soldier — said to have only recently arrived in the country — is accused of walking off his base in Kandahar province in the middle of the night and gunning down at least 16 villagers, mostly women and children.

A U.S. official said the accused soldier had suffered a traumatic brain injury while on a previous deployment in Iraq.

The shootings, which came just weeks after deadly protests across the country over the inadvertent burning of Korans by U.S. soldiers, triggered a protest by around 2,000 students in the eastern city of Jalalabad.

The demonstrators chanted “Death to America” and demanded Afghan President Hamid Karzai reject plans to sign a strategic pact with Washington that would allow U.S. advisers and possibly special forces to remain in the country beyond the planned withdrawal in 2014.

U.S. President Barack Obama, speaking after a phone call with Karzai — who is said to be furious over the latest deaths — said the shootings had only increased his determination to get American troops out of Afghanistan.

However, Obama cautioned there should not be a “rush to the exits” for U.S. forces who have been fighting in Afghanistan since late 2001 and that the drawdown set for the end of 2014 should be done in a responsible way.