Greater Western Sydney will move on from the disappointment of losing star player Toby Greene to suspension for Saturday's preliminary final and will put all their energy into taking on Collingwood and trying to reach their first AFL grand final.

Greene will not play against the Magpies after failing to have his one-match ban overturned at the AFL appeals board on Thursday night. He has been replaced in the Giants' side by former Pie Lachie Keeffe.

Toby Greene was in Melbourne for his appeal hearing. Credit:AAP

In an exhaustive two-hour hearing, Greene's defence team put forward a variety of arguments why he should be cleared and flagged that they would argue that the Giant's contact to Lions star Lachie Neale was "careless" rather than "intentional" as a "last resort option".

But the three-member appeals board dismissed the case on the "balance of probabilities" after deliberating for just 15 minutes.