Tourists have described in court details of an allegedly violent confrontation between two guides on a fishing tour in the Top End.

Allan Ross Beale is defending the charges of assaulting a worker and acting in a way that risked a person's safety.

He is accused of verbally abusing and attempting to punch another operator, Kaleen Qaiser, on the Mary River near Shady Camp on March 29, 2014.

Prosecutor Ray Murphy told the Darwin Magistrates Court the defendant was angry Mr Qaiser ran over another boat's fishing line earlier in the day, allowing a barramundi to escape.

The alleged incident was witnessed by several paying customers from both charters.

Fisherman Andrew Davey was called to recount the fifth and final day of his tour guided by the alleged victim.

"Allan (Ross Beale) came towards us at speed. When he got within earshot he started yelling at Micky (Mr Qaiser)," he said via video link from interstate.

"It went on for quite some time.

"Allan's mouth didn't stop moving."

Mr Davey said the other boat nudged Mr Qaiser's gently, which combined with the force of the tide, pressed it against the bank.

"Allan reversed his boat at speed at ours. Then the motor came out at one point.

"The language just deteriorated from that point. It was very colourful.

"His stance was very aggressive. It looked like he was going to jump onto our boat.

"He said 'I'll have you on the bank. I'll have you on the boat ramp.

"Micky didn't get a word in."

'Just leave us alone'

Mr Davey told the court Beale's fist clipped Mr Qaiser's hat when he attempted to punch him.

"My words were 'just leave us alone we want to get back to fishing'. My dad said 'you need to be more professional'.

"I'm pretty sure none of us wanted to see Allan at the boat ramp so we called it a day and headed back to camp."

The court heard Mr Davey's brother captured part of the incident on a portable camera but the footage is yet to played in court.

Another witness said Beale threatened to kill Mr Qaiser.

Defence counsel Matthew Hubber said Mr Qaiser had "bullied his way" over two trawling lines and said it was no secret that Mr Qaiser and Mr Beale did not get along.

During cross examination of Mr Davey Mr Beale's defence lawyer, Matthew Hubber, questioned why part of Mr Davey's oral testimony was not included in his statement to police.

The hearing continues.