Together with colleagues from the USA, scientists from the University of Bonn and the research institute Caesar in Bonn have successfully been able to use nanostructures to construct a tiny machine that constitutes a rotatory motor and can move in a specific direction.

The scientist used circular structures from DNA.

The Nano machines include structures of complex proteins and nucleic acids that are powered with chemical energy and can perform directed movements.

The principle is known from nature. For example, bacteria propel themselves forward using a flagellum.

The team of the University of Bonn, the research institute Caesar in Bonn and the University of Michigan (USA) used structures made of DNA nano rings.

The two rings are linked like a chain.

According to Prof. Dr. Michael Famulok from the Life & Medical Sciences (LIMES) Institute of the University of Bonn.





“One ring fulfills the function of a wheel, the other drives it like an engine with the help of chemical energy,”

The tiny vehicle measures only about 30 nanometers (millionths of a millimeter). The “fuel” is provided by the protein “T7 RNA polymerase.”

Coupled to the ring that serves as an engine, this enzyme synthesizes an RNA strand based on the DNA sequence and uses the chemical energy released during this process for the rotational movement of the DNA ring.

“As the rotation progresses, the RNA strand grows like a thread from the RNA polymerase,”

explains lead author Dr. Julián Valero from Famulok’s team. The researchers are using this ever expanding RNA thread, which basically protrudes from the engine as a waste product, to keep the tiny vehicle on its course by using markings on a DNA nano tube track.

Length of the test drive is 240 nanometers

Attached to this thread, the unicycle machine covered about 240 nanometers on its test drive.

According to Famulok

“That was a first go,”. “The track can be extended as desired.”

In the next step the researchers are not only aiming at expanding the length of the route, but also plan more complex challenges on the test track.

At built-in junctions, the nano machine should decide which way to go.

“We can use our methods to predetermine which turn the machine should take,”

says Valero.

Of course, the scientists cannot watch the tiny vehicle at work with the naked eye.

By using an atomic force microscope that scanned the surface structure of the nanomachine, the scientists were able to visualize the interlocked DNA rings.

In addition, the team used fluorescent markers to show that the “wheel” of the machine was actually turning. Fluorescent “waymarkers” along the nanotube path lit up as soon as the nano-unicycle passed them.

Based there upon, the speed of the vehicle could also be calculated: One turn of the wheel took about ten minutes.

That’s not very fast, but nevertheless a big step for the researchers.

“Moving the nanomachine in the desired direction is not trivial,”

says Famulok.

The components of the machine assemble by self-organisation

Of course, unlike macroscopic machines, the nanomachine was not assembled with a welding torch or wrench.

The construction is based on the principle of self-organization. As in living cells, the desired structures arise spontaneously when the corresponding components are made available.

According to Famulok

“It works like an imaginary puzzle,”.

Each puzzle piece is designed to interact with very specific partners. If you bring together exactly these partners in a single vessel, each particle will find its partner and the desired structure is automatically created.

By now, scientists worldwide have developed numerous nanomachines and nanoengines.

But the method developed by Famulok’s team is a completely novel principle.

“This is a big step: It is not easy to reliably design and realize such a thing on a nanometer scale,”

says the famulok.

His team wants to develop more complex nanoengine systems soon.

“This is basic research,” “It is not possible to see exactly where it will lead.”

says Famulok.

With some imagination, possible applications could for instance include molecular computers that perform logical operations based on molecular movements.

Additionally, tiny machines could transport drugs through the bloodstream precisely to where they are required.

“But these are still visions of the future,” says Famulok.