

I know this might seem stupid, as easy as it is to make a spore print, but I have been using the same mycelium I grew from a spore syringe 8 months ago up till now using g2g every month or less. Yields are definitely down from those first few pinsets, but only by about 30% or so.



I just made some sporeprints and got everything for agar work, so I can now start working from fresh genetics, but I was wondering. If this line is still performing pretty well this late in the game, would it be worth it to try cloning a fruit or perhaps an isolate from a clone or mycelium? Or will I soon hit some kind of wall?



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Most would consider what you've already hit as a wall.



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Gonna have to agree with that one....



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I believe he asked a question about cloning fruits.



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If you have already hit the proverbial wall on genetics why bother cloning a 5th, 6th, 7th generation gene?



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If you clone fruit bodies from the grown fruits, then your not resetting you genetics...

If you take a print, and start from spores, you reset...



Atleast thats what my basic knowledge tells me...



Next time, be smart with your g2g's and perform a better tree, and backup system...

For example, you find a banging culture...

You store it in a slant to save the culture as young as possible...



This allows you to take a rice sized sliver out of that slant, and put it onto a plate that could then make 20 other plates...

Then 20 plates could be stored over the year to knock your jars up with...



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Quote:

total said:

If you clone fruit bodies from the grown fruits, then your not resetting you genetics...

If you take a print, and start from spores, you reset...







Yes and no, there's going to be a lot more defective and weak sublines.

But still a chance to find a good one. Overall, tired genetics should be retired.



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Quote:

dmonkey1 said:

I believe he asked a question about cloning fruits.









I believe we gave him an answer.



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Quote:

sixbluntsdeep said:

Quote:

dmonkey1 said:

I believe he asked a question about cloning fruits.









I believe we gave him an answer.







but no solutions



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I think his grain to grain transfers are multispore as he started from a spore syringe. I am curious about this too.

So if you do a shitload of grain to grain which are multispore and then clone an isolate from a fruit, you would still have senescence even though you started from multispore and cloned a isolate after the mycelium started losing vigor?



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I have to say UG now I kinda get why 13's mad at me.... You give a person valid pertinent information that really doesn't go any way but described and people still argue.



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That makes sense because its the same as an isolate but just a bunch of strains fruiting together at the same time.



Another question I have for anybody is with multispore do the strains stay seperate strains or after grain to grain transfers do a few strains join together to become one strain, or less strains? Really a pointless question, Im just curious.

I need to do master slants.



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Quote:

dmonkey1 said:



but no solutions







You seem to be doing even less than those you are criticizing. Quote:





savage.renegade said:

That makes sense because its the same as an isolate but just a bunch of strains fruiting together at the same time.



Another question I have for anybody is with multispore do the strains stay seperate strains or after grain to grain transfers do a few strains join together to become one strain, or less strains? Really a pointless question, Im just curious.

I need to do master slants.







Well if you take grain out, you're taking "x" number of varieties and letting them prosper. If you do it again, you're taking "x" varieties out, and so on. So you're getting fewer genetic differences each G2G you do.



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Thats what i was thinking too, Thanks for clarifying that for me.



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Thank you for all your replies! Yeah, I figured that line was getting a little long in the tooth. I've got a nice dark sporeprint and all the fixins, so will be starting work on isolating fresher genetics soon.



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