Maria Perego, an Italian puppeteer and the creator of Topo Gigio, the l ovable mouse who became famous to American audiences as a frequent guest on “The Ed Sullivan Show” in the 1960s and early ’70s and was known worldwide, died on Thursday in Milan. She was 95.

Her death was announced on her official Facebook page.

Ms. Perego, who worked alongside her husband, Federico Caldura, came up with the 10-inch-tall Topo Gigio in the late 1950s. Topo Gigio was a sort of cross between a puppet and a marionette; three puppeteers, hidden in a black background, moved its body parts with rods.

According to “Sundays With Sullivan: How ‘The Ed Sullivan Show’ Brought Elvis, the Beatles, and Culture to America,” a 2008 book by Bernie Ilson, Mr. Sullivan saw a tape of the puppet from Italian television and booked Topo Gigio for a series of appearances on his popular Sunday-night CBS variety show. The first, the book said, was on April 14, 1963.

Ms. Perego and two other puppeteers were on hand to impart the movements, and a fourth provided Topo Gigio’s voice — but, Mr. Ilson wrote, Mr. Sullivan had not realized that someone would also have to serve as the puppet’s straight man. Mr. Sullivan, who was famously wooden on camera, stepped into that task for the initial appearance, figuring he would arrange for a professional comic to take over for later ones if the bit caught on.