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Laboratory diagnosis of invasive Aspergillosis is based on the identification of characteristic symptoms, patient’s clinical history and specialized tests such as X-ray, blood tests, lung biopsy, serology and culture tests

Few of Aspergillus important as human pathogens

Aspergillus fumigatus

Aspergillus fumigatus

Aspergillus flavus

Aspergillus flavus

Aspergillus niger

Aspergillus niger

Aspergillus terreus

Aspergillus terreus

Aspergillus nidulans

Aspergillus fumigatus is mostly medically important. Disease presentation depends on the immunologic status of the patient.A most severe and often fatal form of aspergillosis is an acute invasive infection of the lung and dissemination to the brain. People may have no symptoms or may have to cough up blood, fever, chest pain, difficulty breathing

Laboratory diagnosis of invasive Aspergillosis

Samples are

sputum

BAL

Bronchial washings

tissues

blood

1.Direct examination

Tissues

GMS, PAS, H&E(It will show fungal septate hyphae)

KOH

Lactophenol cotton blue

2.Culture

Sabouraud dextrose agar (SDA) – Colonies are flat , may be white/Yellow-green/black , Eg:-

Aspergillus flavus – yellow-green

Aspergillus fumigatus – blue-green

Aspergillus niger – initial growth is white, becoming black later on giving ‘Salt and pepper’ appearance

3.Serology

Immunodiffution

ELISA

4.PCR

Detection of Aspergillus DNA in clinical samples

5.Immunoelectrophoresis

ELISA test procedure | Enzyme-Linked ImmunoSorbent Assay (step by step) – https://youtu.be/k-i3w3RaWIE

Cotton blue stain – lactophenol cotton blue technique/fungal identification – mycology – https://youtu.be/vNqioD6oN_U

Candida albicans microbiology – microscopic examination,cuture(laboratory diagnosis) – https://youtu.be/BRO6rN_8JEU

Slide culture technique for fungi:Modified-Faster and Easier Identification of Dermatophytes” – https://youtu.be/ut7s_g1_-Uw

Germ tube test candida albicans procedure – Mycology(easy method)” – https://youtu.be/gZwjnLXlO6M

Nematode eggs identification – https://medilabzone.com/nematode-eggs-identification/