The company’s General Dynamics Information Technology Inc. subsidiary cut 344 positions this month at its call center as open enrollment to obtain health care coverage under the Affordable Care Act expired. Those cuts are not reflected in this year’s Top 50 numbers.

Bank of America Corp. and Verizon Communications had the largest declines, each shedding 200 full-time equivalent positions.

University of Richmond finance professor Tom Arnold, who analyzed the 2014 Top 50 list, said the first thing that hit him was the emergence of Amazon.

“You can really see the impact of Amazon moving operations into Virginia by virtue of its high rank of 10,” he said.

Amazon opened two huge fulfillment centers — in the Meadowville Technology Park in Chesterfield County and another one in Dinwiddie County — in fall 2012.

When Amazon announced plans in 2011 to invest $135 million to open the two sites, it said it expected they would employ a combined total of 1,350 people.

Since the openings, Amazon has more than doubled its full-time workforce here, spokeswoman Rebecca Passo Mikulski said. The company employed the full-time equivalent of 2,100 employees at its Chesterfield center and 1,027 FTEs at the Dinwiddie warehouse.