The Schuyler Sisters are singing at the Super Bowl.

The National Football League said Friday that Phillipa Soo, Renée Elise Goldsberry and Jasmine Cephas Jones, the actresses who originated the roles of Eliza, Angelica and Peggy Schuyler in “Hamilton,” would reunite to perform “America the Beautiful” during the televised pregame show.

The three women are popular among fans of the Broadway musical — at the annual BroadwayCon gathering of theater fans, now underway in New York, some people dress as the characters, and at a recent Sotheby’s auction of Hamilton family documents there was particular interest in correspondence involving the sisters. (Eliza, of course, married Alexander Hamilton, and, after his death in a duel, became a champion of his legacy; Angelica was a confidante of both Alexander and Eliza.)

None of the actresses is still in the show’s cast. Ms. Soo is preparing to star in the title role of a new Broadway musical, “Amélie,” which begins previews March 9; Ms. Goldsberry, who won a Tony award for her performance in “Hamilton,” is now working on the Netflix series “Altered Carbon,” and Ms. Jones has filmed appearances in “Girls” and “Blood Surf.”

The N.F.L. had previously announced that the Super Bowl halftime show would be headlined by Lady Gaga, and that Luke Bryan would sing the national anthem.