Multi-Omics-Mouse: The crew performed routine maintenance on the Multi-Omics-Mouse Cage Units that are being maintained in microgravity, exchanging waste collection equipment and odor filters. Several studies have reported space flight effects on the human immune system, but the relationship between microbiota and immune dysfunction during flight remains unclear. In the Multi-Omics-Mouse investigation, food with and without fructooligosaccharides (FOS) will be used as prebiotics, to determine if they improve the gut environment and immune function. After the flight, researchers will analyze the gut environment (microbiota and metabolites) and immune system of the mice by multi-omics analysis.

Cool Flames Investigation: The crew replaced Multi-user Droplet Combustion Apparatus (MDCA) Fuel Reservoir in support of continued operations for the Cool Flames investigation. Cool Flames provides new insight into the phenomenon where some types of fuels initially burn very hot, then appear to go out, but they continue burning at a much lower temperature, with no visible flames (cool flames). Understanding cool flame combustion helps scientists develop new engines and fuels that are more efficient and less harmful to the environment.

SSRMS LEE A Unable to Latch to MBS Base 3: Robotics operations Tuesday evening were scheduled to include an MT Translate, an SSRMS Walkoff to MBS Base 3, and an SPDM Unstow in preparation for the P12B_B RPCM R&R that was scheduled for Wednesday evening. During the SSRMS Walkoff, ROBO was unable to latch LEE A to MBS Base 3. High motor currents were observed as the latches on the LEE were being driven. The SSRMS is currently in a safe configuration with LEE B grappled to the Node 2 PDGF. The SPDM is still stowed. The Robotics team is in agreement to stand down on operations until a FIT is convened to discuss the LEE latch motor current data in more detail. It should be noted the LEE A latches are not required to support a Dragon release. As a result of the inability to complete Tuesday evening’s planned operations, the RPCM R&R scheduled for Wednesday evening has been postponed.

On-Board Training (OBT) Medical Emergency: The 51S crew reviewed medical emergency procedures and rescuer roles during a medical event requiring Cardiopulmonary Resuscitation (CPR) including emergency medical hardware configuration and determine desired deployed locations. During the training they covered crew communication and coordination of care.

Treadmill 2 (T2) Status: Yesterday the crew reported a clicking noise emanating from T2 that had not been heard previously. The crew took video and audio which were downlinked for ground team evaluation and T2 was declared no-go for exercise. Video and audio revealed no obvious sources so the crew was directed to perform T2 annual maintenance. Again, no sources were identified so the crew was given a go to use T2 but to monitor performance and report any further clicking noises.

Today’s Planned Activities

All activities were completed unless otherwise noted.

50S & 51S Samsung Tablet Battery Status Check and Recharge (if it is less than 80% charged)

Aquapad Double Water Sampling

KUBIK Functional check

Combustion Integrated Rack Front End Cap Close/Open

Combustion Integrated Rack Manifold Bottle Replacement

Combustion Integrated Rack Doors Close/Open

Countermeasures System (CMS) Advanced Resistive Exercise Device (ARED) Cylinder Flywheel Evacuation

T2 Activation and Checkout (ACO)

Treadmill 2 (T2) Yearly AND 6-MONTH MAINTENANCE plus Inspections

Environmental Health System (EHS) Coliform Water Processing

Environmental Health System (EHS) Potable Water Dispenser (PWD) Sample Collect

Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Potable Water Dispenser (PWD) Sample Analysis

Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record

Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Waste Water Bag (WWB) Changeout

In Situ message recording

KUBIK ASI Biomission message

Downlink and stowage

EPO items unstow and camera setup

Food Acceptability Questionnaire – Subject

Handover Feedback Questions

Ultrasound 2 Guided Data Export

Health Maintenance System (HMS) Ultrasound 2 Scan Prep

Health Maintenance System (HMS) Ultrasound 2 Post Exam

Ultrasound 2 HRF Rack 2 Power On

Health Maintenance System (HMS) Ultrasound 2 Scan performed by a Crew Medical Officer (CMO)

Health Maintenance System (HMS) Ultrasound 2 Scan

Lighting Effects Sleep Log Entry – Subject

Multi-user Droplet Combustion Apparatus One Fuel Reservoir Replacement

Multi-Omics-Mouse CBEF A3 Cage Install

Multi-Omics-Mouse CBEF A3 Cage Removal

Multi-Omics-Mouse Water Gel Removal from A3 cage

Multi-Omics-Mouse Cage Maintenance for Water A3 Cage

Multi-Omics-Mouse Cage Cleaning and Maintenance for Food Micro-G first part 1 & 2

Multi-Omics-Mouse Item Gathering

NeuroMapping Experiment Neurocognitive Test – Subject [Failed]

On Board Training (OBT) International Space Station (ISS) Crew Health Care System (CHeCS) Drill

CHeCS. Medical Contingency Drill

On Board Training (OBT) International Space Station (ISS) Crew Health Care System (CHeCS) Drill

Photo/TV Treadmill 2 (T2) Activation and Checkout (ACO) Video Setup & Teardown

Surface Tension Containment MWA Assist

Surface Tension Containment MWA Operations

Surface Tension Containment MWA Preparation

Ultrasound 2 HRF Rack 1 To HRF Rack 2 Transfer

1.0 CTB J0026 Wanted

XF305 Camcorder Setup

PROBIOVIT. Glovebox-S hardware prep.

PROBIOVIT. ТБУ-В No.2 installation and activation at + 37 deg С setting.

Video for episode 5 of “Space Yura and Nura” cartoon

End discharge of the 2nd Orlan Battery Pack 825М3 (stow in ФГБ1ПГО_2_221_1) and start discharge of the 3rd Battery Pack No.1265131133 (00070928R)

Post-EVA SM ПхО and DC1 reconfig to nominal

Preventive Maintenance of SM АСП-О and Progress 436 ACA hatch Sealing Mechanisms after the RS EVA

Vacuum Cleaning area behind MRM1 panels 405, 406

MRM1 СКПФ1, СКПФ2 Dust Filter Replacement (ФГБ1ПГО_2_224_1, bag 436-26 (00071452R). Discard de-installed items. Reflect changes in IMS)

Photography of Plume Impingement and Deposit Monitoring Unit [БКДО] position on MRM2 from SM window No.13

Photography of Plume Impingement and Deposit Monitoring Unit [БКДО] position on MRM2 from SM window No.13, Closeout Ops

Photography of Plume Impingement and Deposit Monitoring Unit [БКДО] position on MRM2 from SM window No.13, Preparation

BIOKARD. Performing experiment (assist)

BIOCARD. Experiment Ops.

Hematocrit Test

Measuring Hematocrit Number.

Hematocrit Test

Hematocrit Hardware Stowage

Photography of the БД-2 treadmill belt edge and screen form of Belt tab

Preparation of Reports for Roscosmos web site and social media

Terminate drying the second pair of gloves

Finish drying the 1st pair of gloves and start drying the 2nd pair

Drying the 3rd pair of gloves

Stowage of suits and gloves after drying

Soyuz 735 crew Sokol leak check without donning ПКО and Kentavr

Terminate Drying Suits 1,2

Sokol Suit Dryout, Suits 1 and 2 Setup for Drying

Drying of the 3rd suit – end, start drying the 3rd pair of gloves

Dryout of the 3rd spacesuit: start, and set up the 1st pair of gloves for drying

ECON-M. Observation and photography

Completed Task List Activities

SABL2 Door Open – Morning

Photo/TV RED SSD Reader deploy

Ground Activities

All activities were completed unless otherwise noted.

MT translation from WS4 to WS6

Three-Day Look Ahead:

Thursday, 08/24: STaARS facility/controller/Nexus Lab install, Lung media fixation, ADSEP cell collection, THC/IMC flow measure

Friday, 08/25: Genes In Space, RR habitat restock, 50S prepack, WHC maintenance, Dragon transfer ops

Saturday, 08/26: Crew off duty, Mouse Habitat maintenance, ADSEP cell collection

QUICK ISS Status – Environmental Control Group: