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The rape of a city teen at the hands of three men ended when she threatened to bite off the penis of one of her assailants, court heard Wednesday.

In a videotaped interview played at the trial of two of her alleged attackers, the then-14-year-old told a child abuse detective the trio took turns sexually assaulting her in the bathroom of a city home.

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But after all three had forced themselves on her one tried to make her perform oral sex, she told Det. Mike Ruark in the Aug. 21, 2014 interview.

“After the third guy was done, he didn’t leave the bathroom,” the teen said in the recording, which the now-18-year-old watched from a remote witness room.

“All three of them came in the bathroom while I was still laying down on the floor.

“I don’t know what guy it was, but one of them was trying to get me to have oral sex with him, and I said no,” she explained to Ruark.

“I said, ‘I will literally bite your d— off if you come near me again.’ And then they all stopped and they walked out of the bathroom.”