Summary: Study reveals how the mechanism for storing olfactory memories differs slightly from erasing unnecessary memories. Understanding how the brain gets rid of unimportant memories could help unlock new avenues of research to better understand memory loss in aging, researchers say.

Source: Scripps Research Institute.

The fragrance of hot pumpkin pie can bring back pleasant memories of holidays past, while the scent of an antiseptic hospital room may cause a shudder. The power of odors to activate memories both pleasing and aversive exists in many animals, from humans to the humble fruit fly.

Scientists on the Florida campus of The Scripps Research Institute (TSRI), writing in the journal Cell Reports, detailed how the intricate biochemical mechanism for storing scent-associated memories differs slightly from a less-understood mechanism for erasing unnecessary memories.

Understanding how brains actively erase memories may open new understanding of memory loss and aging, and open the possibility of new treatments for neurodegenerative disease.

In multiple ways, the processes of forgetting and remembering are alike. In fruit fly models of odor-associated learning, both the saving and erasure of memories involves dopamine activation of the brain cells. This clue in flies is important for understanding the human brain.

“The olfactory systems of flies and humans are actually quite similar in terms of neuron types and their connections,” said study leader Ron Davis, Ph.D., co-chair of TSRI’s Neuroscience Department.

Also, in both cases, activation of the neurons causes them to make an identical messenger molecule, cyclic AMP, leading to a cascade of activity within the cell, either building or breaking down memory storage, added Davis.

“So how do the cells know when they are getting a forgetting signal verses an acquisition signal? That was the huge, perplexing question,” Davis said.

TSRI Professor Kirill Martemyanov, Ph.D., and Staff Scientist Ikuo Masuho, Ph.D., found that a type of signaling protein in neurons played a role. Masuho and Martemyanov screened a panel of these signaling proteins, called G proteins, against cells that expressed two key receptors known to be involved in memory and forgetting.

The TSRI team found one G protein, called G alpha S, that latched on to a neural dopamine receptor called dDA1, associated with memory formation. They found a different G protein, called G alpha Q, linked up with a nearby dopamine receptor called Damb, associated with the machinery of forgetting.

The next question was whether those two different G proteins could be controllers of the fly brain’s memory machinery. To find out, the researchers silenced genes involved in the production of the G alpha Q protein in the flies. The flies with the protein silenced were exposed to odors in aversive situations and sent through mazes to see how well they remembered to turn away in the presence of the scent.

“If you removed G alpha Q, the flies should not forget, and indeed, they did not,” Davis said. “They remembered better.”

It appears in flies that some level of forgetting is a constant, healthy process, he said.

“The idea is, constantly as we learn information, there is a slow process that whittles away memories, and it continues whittling them away unless another part of the brain signals the memory is important and overrides it,” Davis said.

It may be that the process of acquiring and forgetting memories ebbs and flows in a state of balance, he said. Important memories like the taste of mom’s pumpkin pie might be forever retained, but trivialities like what you wore 10 years ago can fade into oblivion without consequence.

“If you have too much memory that is old and unnecessary, why keep them around? Why shouldn’t you have a system for removing those for optimal function of the brain?” Davis asked. “We’re getting all this information, all this learning during the day, and the brain may be saying, ‘No, no, bring me back to my basal, my happy state.’”

Many questions remain to be solved, Davis noted. “We need to figure out what is downstream—walk down the pathway to find the complete signaling system for forgetting,” he said. “We are very early in this research.”

About this neuroscience research article

In addition to Davis, other authors of the study, “Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila,” include first authors Sophie Himmelreich and Ikuo Masuho, as well as Jacob A. Berry, Courtney MacMullen, Nickolas K. Skamangas and Kirill A. Martemyanov.

Funding: Research in the Davis laboratory was supported by grants 4R37NS019904, 5R01NS052351, and 1R35NS097224 from NINDS. Research in the Martemyanov laboratory was supported by grants DA036596 and DA026405 from NIDA, and MH105482 from NIMH. The authors thank TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947 ) and Janelia Farms for providing transgenic fly stocks used in this study.

Source: Scripps Research Institute

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Original Research: Full open access research for “Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila” by Sophie Himmelreich, Ikuo Masuho, Jacob A. Berry, Courtney MacMullen, Nickolas K. Skamangas, Kirill A. Martemyanov, and Ronald L. Davis in Nature Nanotechnology. Published online November 21 2017 doi:10.1016/j.celrep.2017.10.108

Cite This NeuroscienceNews.com Article

[cbtabs][cbtab title=”MLA”]Scripps Research Institute “To Forget or to Remember? Memory Depends on Subtle Brain Signals.” NeuroscienceNews. NeuroscienceNews, 22 November 2017.

<https://neurosciencenews.com/memory-brain-signals-8002/>.[/cbtab][cbtab title=”APA”]Scripps Research Institute (2017, November 22). To Forget or to Remember? Memory Depends on Subtle Brain Signals. NeuroscienceNews. Retrieved November 22, 2017 from https://neurosciencenews.com/memory-brain-signals-8002/[/cbtab][cbtab title=”Chicago”]Scripps Research Institute “To Forget or to Remember? Memory Depends on Subtle Brain Signals.” https://neurosciencenews.com/memory-brain-signals-8002/ (accessed November 22, 2017).[/cbtab][/cbtabs]

Abstract

Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila

Highlights

•The dopamine receptor dDA1 couples to Gs for intracellular signaling

•The dopamine receptor Damb couples to Gq for intracellular signaling

•Gαq knockdown with RNAi in the mushroom bodies inhibits forgetting

•Dopamine spurs acquisition through dDA1/Gs and forgetting through Damb/Gq

Summary

Prior studies have shown that aversive olfactory memory is acquired by dopamine acting on a specific receptor, dDA1, expressed by mushroom body neurons. Active forgetting is mediated by dopamine acting on another receptor, Damb, expressed by the same neurons. Surprisingly, prior studies have shown that both receptors stimulate cyclic AMP (cAMP) accumulation, presenting an enigma of how mushroom body neurons distinguish between acquisition and forgetting signals. Here, we surveyed the spectrum of G protein coupling of dDA1 and Damb, and we confirmed that both receptors can couple to Gs to stimulate cAMP synthesis. However, the Damb receptor uniquely activates Gq to mobilize Ca2+ signaling with greater efficiency and dopamine sensitivity. The knockdown of Gαq with RNAi in the mushroom bodies inhibits forgetting but has no effect on acquisition. Our findings identify a Damb/Gq-signaling pathway that stimulates forgetting and resolves the opposing effects of dopamine on acquisition and forgetting.

“Dopamine Receptor DAMB Signals via Gq to Mediate Forgetting in Drosophila” by Sophie Himmelreich, Ikuo Masuho, Jacob A. Berry, Courtney MacMullen, Nickolas K. Skamangas, Kirill A. Martemyanov, and Ronald L. Davis in Nature Nanotechnology. Published online November 21 2017 doi:10.1016/j.celrep.2017.10.108

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