By Jose Sepulveda, Buzz60

A rare species of tree frog seems to now be extinct after the death of its last known living member in captivity.

The frog called Toughie, a Rabbs' Fringe-Limbed Tree Frog, died in its enclosure at the Atlanta Botanical Garden. The frog, believed to be 12 years old, was found dead during a routine daily inspection at the end of September.

In 2005, a team of scientists collected live animals in Panama as an infectious disease in amphibians, threatened species in Central Panama.

Studies suggest that up to 85 percent of the amphibians found on Toughie's home turf were gone and it is unlikely that any of his kind survived in the wild.

See images of other animals on the brink of extinction

5 PHOTOS Animals that face extinction by 2100 See Gallery Animals that face extinction by 2100 Western lowland gorilla Kamba holds her one-day-old son Zachary at the Brookfield Zoo in Brookfield, Illinois, United States, September 24, 2015. REUTERS/Jim Young A pair of black rhinoceros walk at the Imire Rhino and Wildlife Conservation Park near Marondera, east of Zimbabwe's capital Harare, September 22, 2014. Monday marked World Rhino Day amid dwindling populations of the species due to poaching activities. REUTERS/Philimon Bulawayo (ZIMBABWE - Tags: ANIMALS ENVIRONMENT) UNSPECIFIED - OCTOBER 28: African Wild Ass (Equus africanus) (Photo by DEA / F. PAGNI/De Agostini/Getty Images) The Visayan warty pigs, Samar (front) and Panay run at their enclosure on January 11, 2013 at the Berlin zoo. The pigs, priginating from the Polish Poznan Zoo are named after two Philippine islands. Today, the endangered species live only on two islands. AFP PHOTO / MARC TIRL /GERMANY OUT (Photo credit should read MARC TIRL/AFP/Getty Images) Bali banteng (Bos javanicus), calf. Garig Ganuk Barlu National Park, Cobourg Peninsula, Arnhem Land, Northern Territory, Australia. (Photo by Auscape/UIG via Getty Images) Up Next See Gallery Discover More Like This HIDE CAPTION SHOW CAPTION of SEE ALL BACK TO SLIDE

This only exacerbates the fact but other amphibian species are also at risk of getting wiped out.

Scientists estimate that up to one-half of amphibian species all over the world are at risk of extinction.