Metro Trains staff are set to strike again as part of a "week of industrial action", which will include a ban on checking myki tickets on grand final day.

As part of its pay battle with Metro, the Rail, Tram and Bus Union said it would hold a four-hour stop work meeting next Thursday October 1 between 10am and 2pm.

A person has been hit by a train in between Flagstaff and Southern Cross station. Credit:Penny Stephens

The union also pledged to not check tickets on trains on grand final day. The action was lodged with the Fair Work Commission on Wednesday afternoon.

Union secretary Luba Grigorovitch said Metro had failed to address concerns from staff about working conditions during ongoing negotiations that have dragged on for months.