An election campaign ad in which federal MP Bob Katter is depicted apparently shooting an ALP and LNP member has drawn criticism for its release just days after a mass shooting in Orlando.

The minute-long video, uploaded to Mr Katter's social media accounts, begins with two men, one dressed in an ALP shirt and the other in an LNP shirt, erecting an 'Australia for sale' sign.

The video ends with Mr Katter smiling, holding a gun and blowing smoke from the barrel as the camera shows two men lying on the ground.

Forty-nine people were killed and 53 injured when a heavily armed gunman opened fire and seized hostages at the Pulse nightclub in Florida on Sunday morning (local time).

Mr Katter's video has split opinion online.

"Maybe you shouldn't be waving guns around several days after a horrific mass murder," one Twitter user wrote.

"About time a politician saw sense. Keep up the good work Bob," a Facebook user wrote.

Mr Katter rejected the suggestion that he killed off the ALP and LNP members in the ad.

"I'm not giving an explanation, you can go and watch the advertisement because it's anti-selling off Australia advertisement," he told the ABC.

"I think most people will enjoy the humour. I thought it was very funny, I must admit."

He said he was not deliberately controversial.

"It was just for humour value. But it was humour getting a message across," he said.

"What's not funny is the selling off of this country. We hate it and we're tenaciously opposed to it."

Mr Katter said the advertisement was "our idea" but they had help from satirical newspaper Betoota Advocate.

When asked if the timing of the video was inappropriate, just days after the Orlando shooting, Mr Katter said he signed off on the video at least two weeks ago and that he was "no idea when the thing went out".

"As far as I knew it went into the media two weeks ago ... whether it went yesterday or an hour ago I've got no idea ... what I can tell is you I signed off a document authorising it to go public two weeks ago, something like that. Most certainly over a week ago," he said.

"It most certainly wasn't under my control."

Labor senator Penny Wong, who has not seen the ad, told 612 ABC Brisbane she was "always uncomfortable with political ads which choose to, even in a satirical sense, make fun of violence".

"I think it's problematic," she said.

"He often does [go too far]. I just think there's a place for decent debate around issues and I'm up for an argument."