It had been more than 24 hours since their latest injury, so perhaps the Yankees were playing on borrowed time.

Shortstop Troy Tulowitzki became the latest to join the walking wounded, exiting Wednesday’s series finale against the Tigers after three innings with a low-grade left calf strain. He was sent to New York- Presbyterian hospital for further testing.

“That will almost certainly be an IL [scenario], but I haven’t talked through it all yet,” manager Aaron Boone said.

It was unclear when Tulowtizki suffered the injury. Tyler Wade, who entered the game for him at second base with Gleyber Torres sliding over to shortstop, said Tulowitzki came up to him after popping out to end the second inning and told him, “Get loose.”

The Yankees signed the 34-year-old Tulowitzki in January to fill the gap at shortstop until Didi Gregorius returned from Tommy John surgery. The five-time All-Star sat out all of 2018 after having surgery on both of his heels and missed more than half the season in 2017 with ankle and hamstring injuries.

Tulowitzki made it through spring training healthy but lasted only five regular-season games before his lower-body injury woes came back. In the limited sample size, he went 2-for-11 with a home run, four strikeouts and two walks.

With Tulowitzki sidelined, Torres may be headed back to his natural position for the foreseeable future. The 22-year-old came up through the minors as a shortstop but has made 108 of his 125 career major league starts at second base. Gregorius, meanwhile, is not due back until at least June.

“It’s crazy, but I think it’s part of the game,” Torres said of the mounting injuries. “… I feel really comfortable in both positions. Before the game, I prepare really well for if I play second or short. Just try to do my job and help my team.”

Before Wednesday, the Yankees already had a crowded injured list with 10 players, including Giancarlo Stanton, Miguel Andujar, Aaron Hicks, Gregorius, Luis Severino, CC Sabathia and Dellin Betances.

“It sucks. It’s a tough one,” Aaron Judge said. “But any adversity like this makes a team stronger. This is getting a lot of guys some valuable playing time. It’s going to help us out. In spring training, when we had all those guys in there, we told everybody, ‘Hey, everybody’s going to make an impact on this team somehow. … Just stay ready and help us win some ballgames.’”

Wade was recalled from Triple-A Scranton/Wilkes-Barre Monday when Andujar went on the IL with a small labrum tear, but now the Yankees’ infield depth may get tested even more. They currently have five healthy infielders on their 25-man roster, though two, Luke Voit and Greg Bird, have only ever started at first base in their major league careers. Bird has been taking ground balls at third base recently in case of emergency, which might be inching closer.

The only other healthy infielder on the Yankees’ 40-man roster is Thairo Estrada, a 22-year-old who was set to open the season at Triple-A Thursday.

“He’s a guy that we feel like is going to be a good player,” said Boone, who added they had not yet talked about a potential call-up. “He has made a lot of strides and looked really good in the spring.”