The film stars Andrew Garfield as the webslinger and Emma Stone as his love interest.

Cooper, who won a best supporting actor Oscar for 2002's Adaptation, joins an all-star cast of villains that includes Jamie Foxx and Paul Giamatti.

The screenplay is by Alex Kurtzman & Roberto Orci and Jeff Pinkner based on a previous draft by James Vanderbilt. The film will be produced by Avi Arad and Matt Tolmach.

The sequel is set for release May 2, 2014.

Cooper, whose credits also include The Muppets, Syriana and American Beauty, next will be seen in August: Osage County. He is repped by Untitled Entertainment.

Email: Tatiana.Siegel@THR.com, Twitter: @TatianaSiegel27