Commuters will face disruptions to services on Sydney's inner west light rail line next week after tram workers decided to strike over concerns about safety and proposed changes to shifts.

A four-hour work stoppage will occur between 10am and 2pm on Thursday in the wake of negotiations between the Rail Tram and Bus Union and operator Transdev over a new enterprise agreement breaking down.

As part of the protected industrial action, tram workers refused to wear uniforms to work on Friday.

The tram line runs from Central Station to the city's inner west via Pyrmont, Glebe and Lilyfield.