Queensland's largest hospital has recorded one of the country's highest rates of infection from a potentially deadly superbug for the second year in a row.

The Royal Brisbane and Women's Hospital recorded 2.18 serious blood infections from the golden staph bacterium per 10,000 bed stays in 2013-14, the second worst figure in the country for major hospitals.

The Royal Brisbane and Women's Hospital has the nation's second highest golden staph infection rate. Credit:Michelle Smith

Only St Vincent's Private Hospital in Sydney had a worse infection rate, with 2.32 cases per 10,000 stays.

Both hospitals' figures, released on Thursday by the National Health Performance Authority, were above the national threshold of two cases per 10,000 bed days.