Advocates are interrupting operations at Melbourne’s container port to protest against Australia’s border regime and to call for an end to the detention of refugees.

Three climbers have suspended themselves over the entries on Footscray Road and are refusing to come down.

The protesters are carrying banners which read “SOS Manus”.

Three climbers have suspended themselves over the entries on Footscray Road and are refusing to come down. (9NEWS)

All traffic in and out of the port is suspended. (9NEWS)

All traffic in and out of the port is suspended.

"Today, we are taking action against Australia’s border policy which remains open to corporate profits, yet is violently closed to those seeking safety," SOS Manus spokeswoman Charlotte Lynch said.

"We support the calls made by refugees and ex-detainees for international sanctions to be placed on Australia until the people on Manus are free.”

The advocates are carrying banners which read “SOS Manus”. (9NEWS)

Ms Lynch said the protesters were standing in solidarity with the resistance of the men on Manus.

Sid Littlewood, who is one of three climbers, said the group refused to remain complicit in the Australian government’s “torture and imprisonment of refugees”.

“The bipartisan policy of deterrence relies on suffering and abuse to hold up the fiction of Australia’s border.

The protest has caused major traffic delays during morning peak hour. (9NEWS)

“This policy is taking lives, not saving them.”