A South Australian animal charity has lost its licence over allegations that funds it raised were associated with online casinos.

Liquor and Gambling Commissioner Dino Soulio has revoked the licence of Help Save the Furry Ones (HSFO), which its website describes as a non-profit, volunteer-driven cat shelter.

He said Consumer and Business Services (CBS) received information alleging some of the funds raised by HSFO were being transferred to a company associated with online casinos.

Mr Soulio said investigations also indicated that other accounts associated with the charity had not been audited, which is a requirement under the Collection for Charitable Purposes Act.

"The allegations have been put to the licence holder but she has failed to respond to any contact from CBS," he said.

"In this case, I have made a determination that the funds being raised for a charitable purpose were being mismanaged or misapplied, and the licence was revoked."

The ABC has tried contacting the organisation.

Its website said it gave cats veterinary care and kept them at its shelter for as long as it took to "find them a loving home".

"We do not receive any government funding, running purely on donations and unpaid volunteers, yet in the short time we have been operating, we have managed to find happy homes and svae the lives of hundreds of cats that have come through our doors," the website said.

Mr Soulio said the operator of HSFO was no longer allowed to solicit funds.

"I would warn South Australians not to donate to this organisation," he said.