CARACAS, Venezuela  The Bolivian president, Evo Morales, ordered the American ambassador, Philip S. Goldberg, on Wednesday to leave the country, accusing him of supporting rebellious groups in eastern regions that have been rocked by intensifying protests this week.

The expulsion order signals a low point between Bolivia and the United States. Their dealings of late have reflected a heightening tension over American antinarcotics policies and the granting of asylum in the United States to Bolivian officials who fled the country earlier in this decade.

“We do not want people here who conspire against democracy,” Mr. Morales said in Bolivia’s capital, La Paz, announcing the decision to expel Mr. Goldberg. Mr. Morales, a leftist whose top ally is President Hugo Chávez of Venezuela, repeated contentions that Mr. Goldberg was helping groups seeking greater political autonomy in eastern Bolivia.

Officials at the American Embassy in Bolivia, which has repeatedly denied Mr. Morales’s assertions, did not return calls seeking comment. But the State Department said Wednesday evening that it had received no official notification of Mr. Morales’s order expelling Mr. Goldberg, who served as chief of mission to Kosovo before his nomination as ambassador to Bolivia in 2006.