A House of Commons debate on a petition calling for a second EU referendum will take place on Monday, 5 September.

The Commons Petitions Committee confirmed the record-breaking online petition, signed by more than four million people, will be put forward for debate.

The petition, which was set up by a Brexit supporter before the referendum was held, called for the Government to annul the results if the Remain or Leave vote won by less than 60 per cent on a turnout of less than 75 per cent.

A House of Commons spokesman said in a statement: “The Committee has decided that the huge number of people signing this petition means that it should be debated by MPs.

“The Petitions Committee would like to make clear that, in scheduling this debate, they are not supporting the call for a second referendum.

“The debate will allow MPs to put forward a range of views on behalf of their constituents. At the end of the debate, a Government Minister will respond to the points raised.”

The spokesman also said the debate does not have the power to change the law and will not end with the Commons deciding whether or not to have a second referendum.

He added: “The petition – which was opened on 25 May, well before the referendum – calls for the referendum rules to be changed. It is now too late for the rules to be changed retrospectively.

"It will be up to the Government to decide whether it wants to start the process of agreeing a new law for a second referendum.”

Brexit - Some Leave voters are regretting their vote

The Government previously rejected the petition's call for a second referendum on EU membership. In an official reply, the Foreign Office said 33 million people had had their say and "the decision must be respected".

"We must now prepare for the process to exit the EU," it added.

The petiton was disowned by its original author, Leave activist William Oliver Healey, who said his opinions on the need for a high turnout and high level of support for one side had been “hijacked” by EU supporters.

Brexit protest: Thousands march in London Show all 12 1 /12 Brexit protest: Thousands march in London Brexit protest: Thousands march in London A woman poses with a home-made European Union flag as Remain supporters gather on Park Lane in London to show their support for the EU in the wake of Brexit PA Brexit protest: Thousands march in London Remain supporters demonstrate in Parliament Square PA Brexit protest: Thousands march in London Tens of thousands of people gathered to protest the result of the EU referendum PA Brexit protest: Thousands march in London A majority of people in the capital voted to remain in the European Union Reuters Brexit protest: Thousands march in London Protesters chanted: “What do we want to do? Stay in the EU” PA Brexit protest: Thousands march in London The march follows a similar rally in Trafalgar Square that was cancelled due to heavy rain – but which tens of thousands of people turned up to anyway Reuters Brexit protest: Thousands march in London Britain voted to leave the European Union in a referendum by 52 per cent to 48 per cent Reuters Brexit protest: Thousands march in London But support for the Leave campaign in urban areas and among young people was significantly lower Rex features Brexit protest: Thousands march in London Marchers gathered at Park Lane at 11am and marched towards Parliament Square PA Brexit protest: Thousands march in London Some protesters held up baguettes in a display of affection for our continental neighbours PA Brexit protest: Thousands march in London The disparity between different parts of the country has promoted a four million signature petition calling for a second referendum and even a renewed push for Scotland to cede from the UK PA Brexit protest: Thousands march in London The event’s organiser, King’s College graduate Kieran MacDermott, wrote: “We can prevent Brexit by refusing to accept the referendum as the final say and take our finger off the self-destruct button" Reuters

More than 1,000 lawyers have signed a letter addressed to Prime Minister David Cameron saying the result of the referendum is merely "advisory" and not legally binding.

The lawyers add that the Government should organise an independent investigation into the costs and benefits of withdrawal before making plans to exit the bloc.

Four out of 10 people would like to see a second referendum before Britain leaves the EU, according to an opinion poll for The Independent.

Meanwhile, Government lawyers say the decision to begin the process of leaving the EU can be made using an executive order from the new Prime Minister.