Mair, a former online communications director at the Republican National Committee who also worked on behalf of Wisconsin Gov. Scott Walker's presidential campaign earlier this year, has formed Trump Card LLC "to defeat and destroy" Trump's candidacy, according to the report.

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“In the absence of our efforts, Trump is exceedingly unlikely to implode or be forced out of the race,” said an internal memo that the Journal obtained.

“The stark reality is that unless something dramatic and unconventional is done, Trump will be the Republican nominee and Hillary Clinton will become president.”

News of the recently formed group comes a day after the super-PAC supporting Ohio Gov. John Kasich, another GOP presidential rival, announced $2.5 million for TV, radio and other ads over two months targeting Trump in New Hampshire, where the real estate tycoon maintains a solid lead.

Another group, the economic think tank Club for Growth, has already hit Trump with $1 million in ads in the early-voting state of Iowa.

The moves underscore growing concern among Republicans that Trump's bid for the GOP presidential nomination, which at first was considered a long-shot, has moved closer toward becoming a reality as he leads national polls with less than three months before the first votes are cast in the 2016 race.

Mair described Trump Card LLC to the Journal as "loosely organized and highly confidential."

She said she hopes to lock up funds from donors who are interested in pushing back against Trump but don't necessarily want their involvement to become publicly known.