1. Blackman, M. J. Malarial proteases and host cell egress: an ‘emerging’ cascade. Cell Microbiol. 10, 1925–1934 (2008).

2. Yeoh, S. et al. Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes. Cell 131, 1072–1083 (2007).

3. Collins, C. R. et al. Malaria parasite cGMP-dependent protein kinase regulates blood stage merozoite secretory organelle discharge and egress. PLoS Pathog. 9, e1003344 (2013).

4. Withers-Martinez, C. et al. The malaria parasite egress protease SUB1 is a calcium-dependent redox switch subtilisin. Nat. Commun. 5, 3726 (2014).

5. Das, S. et al. Processing of Plasmodium falciparum merozoite surface protein MSP1 activates a spectrin-binding function enabling parasite egress from RBCs. Cell Host Microbe 18, 433–444 (2015).

6. Hale, V. et al. Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress. Proc. Natl Acad. Sci. USA 114, 3439–3444 (2017).

7. Koussis, K. et al. A multifunctional serine protease primes the malaria parasite for red blood cell invasion. EMBO J. 28, 725–735 (2009).

8. Silmon de Monerri, N. C. et al. Global identification of multiple substrates for Plasmodium falciparum SUB1, an essential malarial processing protease. Infect. Immun. 79, 1086–1097 (2011).

9. Collins, C. R., Hackett, F., Atid, J., Tan, M. S. Y. & Blackman, M. J. The Plasmodium falciparum pseudoprotease SERA5 regulates the kinetics and efficiency of malaria parasite egress from host erythrocytes. PLoS Pathog. 13, e1006453 (2017).

10. Ruecker, A. et al. Proteolytic activation of the essential parasitophorous vacuole cysteine protease SERA6 accompanies malaria parasite egress from its host erythrocyte. J. Biol. Chem. 287, 37949–37963 (2012).

11. Miller, S. K. et al. A subset of Plasmodium falciparum SERA genes are expressed and appear to play an important role in the erythrocytic cycle. J. Biol. Chem. 277, 47524–47532 (2002).

12. Thomas, J. A. et al. Development and application of a simple plaque assay for the human malaria parasite Plasmodium falciparum. PloS ONE 11, e0157873 (2016).

13. Glushakova, S., Yin, D., Li, T. & Zimmerberg, J. Membrane transformation during malaria parasite release from human red blood cells. Curr. Biol. 15, 1645–1650 (2005).

14. Glushakova, S. et al. New stages in the program of malaria parasite egress imaged in normal and sickle erythrocytes. Curr. Biol. 20, 1117–1121 (2010).

15. Wickham, M. E., Culvenor, J. G. & Cowman, A. F. Selective inhibition of a two-step egress of malaria parasites from the host erythrocyte. J. Biol. Chem. 278, 37658–37663 (2003).

16. Abkarian, M., Massiera, G., Berry, L., Roques, M. & Braun-Breton, C. A novel mechanism for egress of malarial parasites from red blood cells. Blood 117, 4118–4124 (2011).

17. Taylor, H. M. et al. The malaria parasite cyclic GMP-dependent protein kinase plays a central role in blood-stage schizogony. Eukaryot. Cell 9, 37–45 (2010).

18. Glushakova, S., Mazar, J., Hohmann-Marriott, M. F., Hama, E. & Zimmerberg, J. Irreversible effect of cysteine protease inhibitors on the release of malaria parasites from infected erythrocytes. Cell Microbiol. 11, 95–105 (2009).

19. Collins, C. R. et al. Robust inducible Cre recombinase activity in the human malaria parasite Plasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle. Mol. Microbiol. 88, 687–701 (2013).

20. Jones, M. L. et al. A versatile strategy for rapid conditional genome engineering using loxP sites in a small synthetic intron in Plasmodium falciparum. Sci. Rep. 6, 21800 (2016).

21. Ribacke, U. et al. Improved in vitro culture of Plasmodium falciparum permits establishment of clinical isolates with preserved multiplication, invasion and rosetting phenotypes. PloS ONE 8, e69781 (2013).

22. Wirth, C. C. et al. Perforin-like protein PPLP2 permeabilizes the red blood cell membrane during egress of Plasmodium falciparum gametocytes. Cell Microbiol. 16, 709–733 (2014).

23. Simmons, D., Woollett, G., Bergin-Cartwright, M., Kay, D. & Scaife, J. A malaria protein exported into a new compartment within the host erythrocyte. EMBO J. 6, 485–491 (1987).

24. Lux, S. E. Anatomy of the red cell membrane skeleton: Unanswered questions. Blood 127, 187–199 (2016).

25. An, X. et al. Identification and functional characterization of protein 4.1R and actin-binding sites in erythrocyte beta spectrin: Regulation of the interactions by phosphatidylinositol-4,5-bisphosphate. Biochemistry 44, 10681–10688 (2005).

26. Karinch, A. M., Zimmer, W. E. & Goodman, S. R. The identification and sequence of the actin-binding domain of human red blood cell beta-spectrin. J. Biol. Chem. 265, 11833–11840 (1990).

27. Deligianni, E. et al. A perforin-like protein mediates disruption of the erythrocyte membrane during egress of Plasmodium berghei male gametocytes. Cell. Microbiol. 15, 1438–1455 (2013).

28. Burda, P. C. et al. A Plasmodium phospholipase is involved in disruption of the liver stage parasitophorous vacuole membrane. PLoS Pathog. 11, e1004760 (2015).

29. Chandramohanadas, R. et al. Apicomplexan parasites co-opt host calpains to facilitate their escape from infected cells. Science 324, 794–797 (2009).

30. Baker, D. A. et al. A potent series targeting the malarial cGMP-dependent protein kinase clears infection and blocks transmission. Nat. Commun. 8, 430 (2017).

31. Holder, A. A. & Freeman, R. R. Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody. J. Exp. Med. 156, 1528–1538 (1982).

32. Withers-Martinez, C. et al. Expression of recombinant Plasmodium falciparum subtilisin-like protease-1 in insect cells: Characterization, comparison with the parasite protease, and homology modelling. J. Biol. Chem. 277, 29698–29709 (2002).

33. Collins, C. R., Withers-Martinez, C., Hackett, F. & Blackman, M. J. An inhibitory antibody blocks interactions between components of the malarial invasion machinery. PLoS Pathog. 5, e1000273 (2009).

34. Blackman, M. J. Purification of Plasmodium falciparum merozoites for analysis of the processing of merozoite surface protein-1. Methods Cell Biol. 45, 213–220 (1994).

35. Mastronarde, D. N. Automated electron microscope tomography using robust prediction of specimen movements. J. Struct. Biol. 152, 36–51 (2005).

36. Kremer, J. R., Mastronarde, D. N. & McIntosh, J. R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116, 71–76 (1996).

37. Shevchenko, A., Tomas, H., Havlis, J., Olsen, J. V. & Mann, M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 1, 2856–2860 (2006).