SpX-14 Mission: After yesterday’s successful capture and berthing of Dragon, the crew ingressed the vehicle early this morning and began cargo transfer operations. Once the crew finishes unloading the center stack, they will transfer SpX-14 cold stowage into ISS assets and begin installation and activation of several payloads delivered by the vehicle.

Mobile Servicing System (MSS) SpX-14 Operations: Later today, ground robotic specialist will release the Dragon vehicle and unstow the Special Purpose Dexterous Manipulator (SPDM). Finally, the Enhanced ORU Temporary Platform (EOTP) will be configured to support tomorrow’s operations to extract the Pump and Flow Control Subassembly (PFCS) and Materials International Space Station Experiment (MISSE) from the Dragon trunk.

Mouse Stress Defense: The crew completed final preparations and then transferred the mice arriving on the SpaceX-14 vehicle from the Transportation Cage Unit to the Cell Biology Experiment Facility (CBEF) to start the Mouse Stress Defense investigation. Microgravity provokes cellular mechanical stresses and perturbs cellular signaling, leading to reduction of muscle and bone density. To overcome these stresses, one of the promising strategies is to activate Nrf2, a master regulator of antioxidant pathway. Mouse Stress Defense investigates how Nrf2 contributes to effective prevention of space-originated stresses.

NanoRacks Plate Reader: The crew validated and calibrated the NanoRacks Plater Reader-2 today. This is in preparation for its use with the Metabolic Tracking-2 investigation. NanoRacks Plate Reader-2 is a laboratory instrument designed to detect biological, chemical or physical events of samples in microtiter plates. Microplate readers are widely used in research, drug discovery, bioassay validation, quality control and manufacturing processes in the pharmaceutical and biotechnological industry and academic organizations.

Multi-Use Variable-G Platform (MVP): The crew installed the MVP hardware that arrived on the SpaceX-14 vehicle into EXPRESS Rack (ER) 8 in the US Laboratory. MVP is used to conduct research in space with a wide variety of sample types, such as fruit flies, flatworms, plants, fish, cells, protein crystals and many others. It includes 2 internal carousels that simultaneously can produce up to 2 g of artificial gravity. Each carousel hosts 6 separate removable experiment modules on each carousel. Video and still imagery, including microscopy, can be included. The platform provides temperature and humidity controls and monitors and records oxygen and carbon dioxide levels.

Tangolab-1 and -2: The crew installed the Tangolab-2 facility in EXPRESS Rack ER 6 in the US Laboratory and performed several payload card placements in the TangoLab-1 & 2 facilities. TangoLab-1 and TangoLab-2 lockers are reconfigurable general research facilities designed for microgravity research and development and pilot manufacturing aboard the ISS.

Today’s Planned Activities

All activities are complete unless otherwise noted.



Crew Quarters (CQ) Door Closing

Dragon Ingress

Regeneration of БМП Ф2 Micropurification Cartridge (start)

SpX-14 Dragon Air Sampling using АК-1М sampler prior to Air Duct Installation

Study of cardiovascular system under measured physical CYCLE Ergometer load.

Cardiovascular evaluation during graded exercises on cycle ergometer

Dragon Arrival Photos

Transfer Center Stack

ISS Crew Orientation

FAZOPEREKHOD. Preparation steps and starting the experiment. XF305 Camcorder Setup

Cell Biology Experiment Facility (CBEF) Mouse Habitat Unit (MHU) Removal

Activation of Laptop RS2

Transfer of ЦВМ and ТВМ Images Version 08.10 to Central Post Computer 2 (КЦП2).

Polar 3 Transfer From Dragon to ISS

Mouse Stress Defense Pre-mission Cage Watering

Photographing the position of the БКДО on MRM2 from the SM window №13 – preparation

Transfer of ЦВМ and ТВМ Images Version 08.10 to Central Post Computer 1 (КЦП1).

Photography of Plume Impingement and Deposit Monitoring Unit [БКДО] position on MRM2 through SM window No.13

Pre-Mouse Stress Defense Item Gathering

СОЖ maintenance

MELFI Overview OBT

Deactivation of RS2 Laptop

Cold Stowage Double Coldbag Unpack 1

FAZOPEREKHOD. Closeout Ops.

CONSTANTA-2. Cassette removal from ТБУ-В and setup on panel for 1-hour warmup

XF305 Camcorder Setup

Mouse Habitat Cage Unit Preparation

Polar and JAXA TCU Dragon Uninstall (with TCU Handover) and Polar Transfer and EXPRESS Rack Install

Mouse Stress Defense Pre-mission Animal Transfer

CONSTANTA-2. Preparation and Execution 4.

Preventive Maintenance of FS1 Laptop (Cleaning and rebooting)

Plate Reader2 Validatin Plate Reader Swap1

Semi-annual BRI maintenance.

Plate Reader2 Validatin Plate Reader Swap2

Polar 4 and Invitrobone Dragon Uninstall and Polar Transfer and EXPRESS Rack Install

Dragon Cargo Operations Conference

Delta file prep

Plate Reader2 Validatin Plate Reader Stow

Divert Unwanted Space Trash Review

INTERACTION-2. Experiment Ops

Testing Inter-modular connectivity (after semi-annual BRI maintenance)

Cold Stowage Double Coldbag Unpack 2

PCG-11 Hardware Photography

Polar Samples to Cold Stowage – Transfer 1

iPad Air 2 Charge

TangoLab-2 Activation and Checkout

TangoLab-1 Card Cube Replace

Utility Outlet Panel (UOP) Activation

Cell Biology Experiment Facility (CBEF) Filter Case Attachment

Multi-Use Variable-G Platform Hardware Install

Dragon Debrief Conference

Regeneration of Micropurification Unit (БМП) Ф2 Absorber Cartridge (end)

Completed Task List Activities

None

Ground Activities

All activities are complete unless otherwise noted.



Dragon Configuration for ISS Operations

MSS Operations to Unstow SPDM & Rotate EOTP

Three-Day Look Ahead:

Friday, 04/06: Veg03 Ops, Metabolic Tracking Setup, Mouse Stress Defense Ops, STP-H5 Imagery, Medical Contingency OBT, MVP Insertion Ops, Made-In-Space Setup, METEOR Ops

Saturday, 04/07: Housekeeping, Crew Off-Duty

Sunday, 04/08: Crew Off-Duty

QUICK ISS Status – Environmental Control Group: