Data are an extension of Fig. 3d–f. a–e, Representative actograms of running wheel activity from individual C3H/HeN wild-type mice kept in constant dark (grey bars) treated with vehicle (a), melatonin (b), UCSF7447 (c), UCSF3384 (d) or luzindole (e). All treatments were 30 μg per mouse except for luzindole, which was 300 μg per mouse as described in the Methods. Mice were treated at dusk (CT 10; 2 h before the onset of running wheel activity) for three consecutive days (black dots). Red lines indicate the best-fit line of pre-treatment onsets and blue lines indicate the best-fit line of post-treatment onsets, which were both used for phase shift determinations (see Methods for more details). The corresponding quantification can be found in Fig. 3d. f–h, Representative actograms of running wheel activity from individual C3H/HeN wild-type mice kept in the constant dark treated with vehicle (f), melatonin (g) or UCSF7447 (h; all treatments were 0.9 μg per mouse) at CT 10. The corresponding quantification can be found in Fig. 3d. i–k, Representative actograms of running wheel activity from individual C3H/HeN wild-type mice kept in the constant dark treated with melatonin (i) at CT 2 (10 h before the onset of running wheel activity) or vehicle (j) compared with UCSF7447 (k; all treatments were 30 μg per mouse) at CT 6 (6 h before the onset of running wheel activity). The corresponding quantification can be found in Extended Data Fig. 7. l–q, Representative actograms of running wheel activity from individual C3H/HeN wild-type (l, m), MT 1 KO (n, o) and MT 2 KO (p, q) mice kept in constant dark treated with vehicle (white; l, n, p) or UCSF7447(blue; m, o, q; 30 μg per mouse) at CT 10. The corresponding quantification can be found in Fig. 3e. r–w, Representative actograms of running wheel activity from individual C3H/HeN wild-type (r, s), MT 1 KO (t, u) and MT 2 KO (v, w) mice kept in constant dark treated with vehicle (white; r, t, v) or UCSF7447(blue; s, u, w; 30 μg per mouse) at CT 2. The corresponding quantification can be found in Fig. 3f. All treatments were given by subcutaneous injection.