(A) Experimental design (formalin test): paw injection of 2% formalin was administered at 0 min; time spent licking paw was measured for 60 min and quantified during the acute phase (0–5 min) and the inflammatory phase (15–45 min).

(B) Time spent licking paw following formalin injection displayed in 5-min time bins in ad libitum-fed (n = 6) and 24-hr food-deprived (n = 6) mice (two-way repeated-measures ANOVA, p < 0.001).

(C) % time spent paw licking during acute and inflammatory phases of formalin test (two-way repeated-measures ANOVA, p < 0.05).

(D) Time spent paw licking during the inflammatory phase of formalin test in ad libitum-fed and 24-hr food-deprived mice (unpaired t test, p < 0.001).

(E) Lick bouts during the inflammatory phase of formalin test in ad libitum-fed and 24-hr food-deprived mice (unpaired t test, p < 0.01).

(F) Time spent paw licking during the acute phase of formalin test in ad libitum-fed and 24-hr food-deprived mice (unpaired t test, p = not significant [ns]).

(G) Lick bouts during the acute phase of formalin test in ad libitum-fed and 24-hr food-deprived mice (unpaired t test, p = ns).

(H) Experimental design (hotplate test): latency to withdraw paw from 52°C hotplate was measured.

(I) Latency to withdraw paw in ad libitum-fed (n = 12) versus 24-hr food-deprived (n = 14) mice during hotplate test (unpaired t test, p = ns).

(J) Experimental design (Von Frey): paw withdrawal from Von Frey filaments was measured.

(K) Withdrawal threshold (Von Frey filament at which mouse responded to >50% of trials) in ad libitum-fed (n = 11) versus 24-hr food-deprived (n = 7) mice (unpaired t test, p = ns). Data are expressed as mean ± SEM, ns p > 0.05, t tests and post hoc comparisons: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; ANOVA interaction: ∞p < 0.05, ∞∞∞p < 0.001; ANOVA main effect of group: ☼☼p < 0.01.