After a career in standup, sketch comedy, movies, and television, Janeane Garofalo will make her Broadway debut in the Roundabout Theater Company’s “Marvin’s Room” this summer.

The play was written by Scott W. McPherson and follows the uneasy reunion between two estranged sisters, one of whom has leukemia. It originally ran Off Broadway in 1991; in a Times review, Frank Rich called it “one of the funniest plays of this year as well as one of the wisest and most moving.” A 1997 film adaptation starred Meryl Streep, Leonardo DiCaprio, Robert De Niro and Diane Keaton.

Ms. Garofalo starred in “Russian Transport” Off Broadway in 2012, but has appeared sparingly onstage over her career — especially compared with her roles in movies and television, including “Wet Hot American Summer,” “24” and “The Truth About Cats and Dogs.” In “Marvin’s Room,” she will portray Lee, the role played by Ms. Streep in the film.

Lili Taylor will play Lee’s ailing sister Bessie, and Celia Weston will be their aunt Ruth. Anne Kauffman will direct in her Broadway debut. Previews begin June 8, with the official opening on June 29, at the American Airlines Theater.