The man accused of gunning down Gambino mob boss Francesco “Frankie Boy” Cali on Staten Island was indicted for murder and ordered held without bail at his Wednesday arraignment.

Anthony Comello, 24, was charged with second-degree murder and two counts of second-degree criminal possession of a weapon at state Supreme Court in the cold-blooded killing of Cali outside the mobster’s Todt Hill home last month.

Cops said Comello open fire with a 9mm pistol after luring Cali, 53, outside by backing a pickup truck into the gangster’s Cadillac Escalade SUV.

Investigators suspect the killing was revenge for Cali ordering his niece not to date Comello, law enforcement sources have said.

Comello pleaded not guilty to the slaying.

The case is due back in court on May 9.