Updated at 3:50 p.m. March 22 to note that a widely cited study about the economic impact of Texas' bathroom bill has been called into question.

LGBT advocates are deploying one more weapon in their fight against Texas' controversial bathroom bill: potty humor.

Not just any potty humor. The ACLU of Texas and nonprofit Legacy Community Health recruited Academy Award-nominated director Richard Linklater of Boyhood fame to make an ad titled "Taking a Seat, Making a Stand."

There's only one way to stop the bill, one man in the ad says.

Another follows: "You've got to roll up your sleeves. Pull down your pants. And pee with LGBT."

Yet another man pipes up: "You've gotta spray it to say it."

The one-minute ad criticizes Lt. Gov. Dan Patrick's transgender bathroom bill, which would let private businesses enforce their own restroom policies.

Restaurants could ban people from using the restroom that matches their gender identity or open up the ladies room to any gender, regardless of whether your city has an ordinance protecting the LGBT community.

The bill would also force people in public schools and state and local government buildings to use the shared bathroom or locker room that matches their "biological sex."

Patrick's proposed legislation, known as Senate Bill 6, is similar to bathroom bills in North Carolina and Indiana. Although the Texas bill makes some concessions — such as exempting arenas and convention centers from restrictions — it has been decried by businesses, law enforcement leaders and civil rights groups as unnecessary and discriminatory.

By various counts, North Carolina's economy has lost between $77 million and $200 million over its bathroom bill. One widely cited study warns that the Texas measure could cost the state $8.5 billion in lost revenue, but fact-checking website PolitiFact found that analysis to be flawed and rated it "mostly false." The Texas Association of Business defended its study by saying that experts had agreed that the bill would have a significant negative impact on the state's economy.

The ACLU ad ridicules the Tar Heel State while men and women list the reasons Texas' bathroom bill shouldn't pass.

"Because SB6 threatens Texas businesses," says a man in a suit who is checking his phone in a restroom.

In the next frame, a man wearing a cowboy hat wipes his hand with a paper towel and throws the wad in the trash: "And because we damn sure ain't going to end up like North Carolina."

A woman in a school hallway tells viewers that money should be spent "keeping kids in school, not out of bathrooms."

GSD&M, an Austin advertising agency, made the ad free of charge, according to Advertising Age.

"Discriminatory legislation is not what this country is about," the CEO of the agency, Duff Stewart, told the magazine. "We need to speak up when we can."

The ad is part of an advertising campaign complete with a website, merchandise and a hashtag: #StopSB6. The campaign prompts people to write to their lawmakers to oppose the bill.

Linklater directed the ad in the GSD&M office and restrooms. He most recently won accolades for the 2015 film Boyhood, a coming-of-age tale shot over 12 years. He's also responsible for the '90s hits Before Sunrise, a romantic drama that spawned two sequels, and Dazed and Confused, a cult classic about a group of kids during their last day of high school.

The Houston-born director courted controversy in recent years with his 2011 black comedy Bernie, a sympathetic portrayal of a real-life mortician who killed his companion, a wealthy East Texas widow. The mortician, Bernie Tiede, fought his prison sentence with Linklater's help in 2014 and temporarily moved into the filmmaker's garage.