In a mosquito responsible for transmission of malaria, heat-seeking behavior - critical to this insect's ability to locate and feed on warm-blooded hosts - relies on a thermoreceptor that was once focused on heat avoidance (to help the mosquito keep cool). Today, the receptor is wired for heat targeting (to help the insect find its next meal). A new study reporting this finding, by suggesting a means to block mosquito heat-seeking, may help guide the development of novel methods for controlling mosquito-borne illnesses like malaria. Of all insect vectors, mosquitoes are perhaps the most famous, responsible for transmitting a host of different pathogens. Efforts to control malaria through vaccines or its mosquito-vectors using pesticides have proven difficult, leading researchers to pursue alternative strategies. Like other disease-spreading insects, mosquitoes use specialized receptors that sense body heat to target the source of their next blood meal. However, the molecular basis of their heat-seeking behavior remains unknown. Chloe Greppi and colleagues evaluated whether ancestral cooling-activated receptors play a role in heat sensing in Anopheles gambiae - the primary mosquito vector responsible for the transmission of malaria in most of sub-Saharan Africa. Using genome-wide analyses and labeled CRISPR-Cas9 mutants, Greppi et al. identified the evolutionarily conserved sensory thermoreceptor IR21a as a key driver ofheat-seeking behaviors. In other insects, Ir21a is a cooling receptor and mediates heat avoidance, allowing the insects to maintain optimal body temperatures. According to the authors, the evolution of blood-feeding in An. gambiae mosquito involved a repurposing of this ancestral thermoreceptor to facilitate warmth sensing instead. While blocking Ir21a did not wholly end heat-seeking behavior outright, it significantly reduced the ability of female mosquitos to find a source of blood. "Thermoreception has been a relatively neglected aspect of vector biology, with research efforts focused largely on chemoreception," writes Claudio Lazzari in a related Perspective. Identifying the root of thermosensation opens research avenues and possibilities for controlling vector-borne disease, Lazzari says.

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