Engineered Heart Tissues: The crew performed sample fixations on selected tissue chambers and performed cell culture media exchanges on the remaining tissue chambers. A Human iPSC-based 3D Micro-physiological System for Modeling Cardiac Dysfunction in Microgravity (Engineered Heart Tissues) assesses human cardiac function in microgravity. It uses 3D cultured cardiac muscle tissue that mimics the architecture and function of adult human cardiac muscle. A magnet-based sensor placed underneath the tissue culture chamber allows real-time, non-destructive analysis of the functional performance and maturation of the tissues in space, which is compared with those cultured on Earth.

BioFabrication Facility (BFF): The crew placed a test cassettes (without cells) into the BFF for a test print. Cleaning Syringes were swapped out after the prints and photos were taken. The BFF is dedicated to manufacturing human organs and tissues in space, primarily for use by patients on Earth. Besides printing tissue, the BFF also can help maintain the health of deep space exploration crews by producing food and personalized pharmaceuticals on demand.

RADI-N2 (Radiation Dosimetry Inside ISS-Neutrons): The crew retrieved the 8 dosimeters from the Columbus module and handed over the units to CDR for data downlink. The objective of this Canadian Space Agency investigation is to better characterize the ISS neutron environment, define the risk posed to the crew members’ health, and provide the data necessary to develop advanced protective measures for future spaceflight. It’s been recognized that neutrons make up a significant fraction (10-30%) of the biologically effective radiation exposure in low-Earth orbit. The bubble detectors used in the investigation are designed detect neutrons and ignore all other radiation.

Completed Task List Activities:

Photo documentation Engineered Heart Tissue (EHT) operations within LSG.

Today’s Ground Activities:

All activities are complete unless otherwise noted.



BioFabrication Facility Test Print

EHT Tissue Chamber Media Change and Fix/Preservation

SM 8.11 loads

Look Ahead Plan



Friday, 3/27 (GMT 087)

Payloads:



Vascular Echo Blood Collection (CSA)

BFF hardware uninstall (NASA)

MVP2 Cell-03 Nutrient tube and waste tube replacement (NASA)

Food Acceptability (NASA)

ISS HAM pass (NASA)

POLAR desiccant swap (NASA)

Everywear App configuration on Ipads (ESA)

Systems:

SpX-20 EVA Pre-pack

Regen ECLSS Operations

Saturday, 3/28 (GMT 088)

Systems:



SpX-20 Cargo Operations

Sunday, 3/29 (GMT 089)

Crew Off-Duty Day

Today’s Planned Activities:

All activities are complete unless otherwise noted.

