Six red clover varieties with diverse architectures and traits have recently been selected as parents of the next year generation, after positive field trails in IBERS last summer that scored plants from around Europe. These activities are part of the project 'Comparative population genomics of red clover domestication and improvement' funded by BBSRC. Credit: Charlotte Jones/IBERS

The Genome Analysis Centre (TGAC) in collaboration with IBERS, has sequenced and assembled the DNA of red clover to help breeders improve the beneficial traits of this important forage crop. The genome is published in Scientific Reports, a journal from the Nature publishing group.

Before industrial nitrogen fertiliser production (from fossil fuels), red clover and other legume crops were essential in crop rotation, improving soil fertility. Legumes boost soil nitrate fertility by assimilating nitrogen from the air, recruiting soil bacteria to help - this is considerably more eco-friendly than the equivalent industrial process.

Indeed, environmental concerns and climate change mitigation is putting red clover back in the spotlight. This requires genetic improvements to help boost its performance.

Red clover's chief benefits (alongside soil improvement) is to provide a protein-rich livestock feed - it also boosts omega-3 fatty acids in ruminant milk. Compared to white clover and other legumes, red clover has high levels of an enzyme that causes its' protein to be digested more slowly and effectively - it's more nutritious per mouthful.

However, currently, red clover only grows well for two or three seasons and it does not recover well from grazing by livestock. It also does not lend itself easily to traditional crop breeding practices, with severe loss of vigour and fertility if inbred. An ongoing project at TGAC and IBERS aims to use a collection of diverse natural lines of red clover for breeding new elite varieties more tolerant to grazing, thus making it more persistent, and to understand the domestication process that led to the adoption of red clover as a crop.

For the breeders, this draft genome provides a welcome tool, with which to speed up incorporation of traits that are beneficial for the clovers' use in sustainable agriculture from natural populations of red clover plants sampled from all over Europe. Widening of the genetic diversity of the breeding populations will help to make red clover a more robust and reliable crop.

Six red clover varieties with diverse architectures and traits have recently been selected as parents of the next-year generation, after positive field trails in IBERS last summer that scored plants from around Europe. These activities are part of the project 'Comparative population genomics of red clover domestication and improvement' funded by BBSRC. Credit: Charlotte Jones/IBERS

Therefore, the genome sequence promises to be a valuable platform for advances in studies of traits of biological and agronomic importance in forage crops.

Lead author Jose de Vega, Researcher at TGAC, said: "The publication of the red clover reference genome is an important milestone, as it represents the first genome sequence of the clover forage crops, which are key components of more sustainable livestock agriculture.

"The availability of the genome assembly will pave the way towards genomics-assisted breeding methods for forage legumes, and provide a platform for deeper understanding of the genetics of forage crop domestication."

"Red clover is attractive because of its high protein content, diversity and ability to fix atmospheric nitrogen, but to enhance its role in sustainable agriculture requires improvements of persistency, disease resistance, and tolerance to grazing."

Senior author and project leader Leif Skøt from the Institute of Biological, Environmental and Rural Sciences (IBERS) at Aberystwyth University said: "The red clover genome puts us in a great position to incorporate and build on this resource. We work closely with Dr David Lloyd, IBERS forage legume breeder, so we are using our genomic tools to assist in improving the precision and speed of breeding better red clover varieties. This is very timely as the importance of legumes in sustainable agriculture is "rediscovered".

Six red clover varieties with diverse architectures and traits have recently been selected as parents of the next year generation, after positive field trails in IBERS last summer that scored plants from around Europe. These activities are part of the project Comparative population genomics of red clover domestication and improvement' funded by BBSRC. Credit: Charlotte Jones/IBERS

"The collaboration between TGAC and IBERS reinforces the UK's leadership in translating the development of genetic and genomic resources from fundamental science to applications with a potential impact on the local and national economy".

The development of a more sustainable agriculture is a key aspect of the UK research strategy, positioning both Institute's as international leaders in biotechnology specifically in the area of forage legumes. This is delivering impact to a broad range of stakeholders and ensuring that the genomics resources will be translated to research and breeding programmes.

The study entitled: "Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement" is published in Scientific Reports, a journal of the Nature publishing group.

This work results from the partnership between, TGAC, IBERS and Germinal Holdings LTD, part funded by the latter through TGAC Capacity and Capability Challenge (CCC) programme, and a Responsive Mode award from the BBSRC.

IBERS and TGAC are strategically funded by BBSRC and TGAC operates a National Capability to promote the application of genomics and bioinformatics to advance bioscience research and innovation.

More information: Jose J. De Vega et al. Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement, Scientific Reports (2015). Journal information: Scientific Reports Jose J. De Vega et al. Red clover (Trifolium pratense L.) draft genome provides a platform for trait improvement,(2015). DOI: 10.1038/srep17394

Provided by The Genome Analysis Centre