We offer the first systematic quantitative meta-analysis on sex differences in humor production ability. We included studies where participants created humor output that was assessed for funniness by independent raters. Our meta-analysis includes 36 effect sizes from 28 studies published between 1976 and 2018 (N = 5057, 67% women). Twenty of the 36 effect sizes, accounting for 61% of the participants, were not previously published. Results based on random-effects model revealed that men's humor output was rated as funnier than women's, with a combined effect size d = 0.321. Results were robust across various moderators and study characteristics, and multiple tests indicated that publication bias is unlikely. Both evolutionary and cultural explanations were considered and discussed.