Yes, no, no and No again.



Yes, these are all the prominent "evil" Villains in the game discarding all the organizations like Volskaya, Lumerico, Vishkar, Null Sector, Space Gorillas, Talon minions, Other Junkers, Los Muertos, etc.



No, because Zarya, Hanzo and Symmetra are sorta Anti-villains or quite volatile and currently unpredictable Neutral Characters. And S: 76, Ana and McCree are Anti-Heroes that are Good for now, but I could still expect a twist from.



No, because other than Widow (unless Talon's secretly good), these guys have their own goals separate from what we expect. Sombra and Reaper are Mercs serving Talon to further their own Goals, Sombra and the Junkers see themselves as Freedom Fighters, Reaper has shady intentions not fully revealed and Widow was brainwashed (and still regrets killing her husband).



The last No is because, due to Overwatch's Terrible Writing, these guys haven't even done anything non-filler yet, so they're only villain-ish, they haven't done much to prove it other than fighting for a bit but their (specifically TALON's) motivations are so vague, people having made theories on how they might be good.