A super PAC, funded by the owners of the Chicago Cubs, has rolled out an attack ad on Donald Trump featuring some of the most misogynist things the billionaire has said over the past 25 years.

The Our Principles PAC issued the minute-long clip on Monday, in which women read out sexist and derogatory comments Trump has made about women, including Princess Diana, Fox News anchor Megyn Kelly, comedian Rosie O'Donnell, his wife Melania, and former Republican rival Carly Fiorina.

The group received a $3million donation from Marlene Ricketts, whose family owns the Cubs, according to the New York Times. Her husband Joe Ricketts is the billionaire founder of TD Ameritrade.

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Donald Trump (pictured at a rally in Vienna, Ohio, on Monday) comes under fire in a video that references some of the most misogynist things he has said in the past two decades

The ad begins with the words 'bimbo', 'dog' and 'fat pig' - insults that Trump has hurled at Kelly, New York Times columnist Gail Collins and O'Donnell respectively.

Each quote is backed up by the name of several media outlets who reported Trump's quotes, along with the date of publication.

The clip also features numerous derogatory comments the GOP frontrunner has made towards women's physical appearances.

For instance, once when speaking about O’Donnell, he said: 'I'd look her right in that fat ugly face of hers.’

Trump used a similar line against Fiorina when she was running against him for the GOP nomination. He said: 'Look at that face. Would anyone vote for that?'

And even Princess Diana was not immune to Trump's vitriol. He once remarked: 'She had the height, she had the beauty, she had the skin. She was crazy, but these are minor details.'

In the video (pictured), seven women read out comments Trump made regarding Princess Diana, Fox News anchor Megyn Kelly, comedian Rosie O'Donnell, his wife Melania, and women in general

Marlene Ricketts (third from the left) donated $3 million to the Our Principles PAC. A source says funding for an anti-Trump add campaign came from other donors. She is pictured with her family, who owns the Chicago Cubs, including husband Joe (second left) and son Tom (fifth from the left)

In 2005, Trump revealed he wanted more children with his wife Melania (pictured with him earlier this month in Detroit, Michigan) but intended to do absolutely no work to raise them. 'I like kids,' he said. 'I mean, I won't do anything to take care of them. I'll supply funds and she'll take care of the kids'

In 2005, he revealed he wanted more children with his wife Melania, but clarified that he intended to make no effort towards raising them – other than provide the ‘funds’.

'I like kids,' he said. 'I mean, I won't do anything to take care of them. I'll supply funds and she'll take care of the kids.'

The video also references an infamous comment he made about Kelly after she grilled him for his misogyny during a Republican debate in August last year.

'You could see there was blood coming out of her eyes, blood coming out of her wherever,' Trump said in a CNN interview.

Another quote comes from a 2013 episode of Celebrity Apprentice, in which he told contestant Brande Roderick: 'That must be a pretty picture, you dropping to your knees.'

In a 1991 interview with Esquire, Trump said: 'You know, it really doesn't matter what they write as long as you've got a young and beautiful piece of ass.'

Trump attacked the Ricketts family in a tweet last month, after reports they had donated to the super PAC

Chicago Cubs owner Tom Ricketts (pictured) said it's a little 'surreal when Donald Trump threatens your mom' after the billionaire posted a tweet about the family

At the end of the video, the women say: 'This is how Donald Trump talks about our mothers, our sisters, our daughters. If you believe America deserves better, vote against Donald Trump.'

A source close to the Our Principles PAC told USA Today at the end of January that other donors, rather than the Ricketts family, had funded a new round of anti-Trump advertising.

Trump attacked the family in a tweet last month, writing: 'I hear the Rickets family, who own the Chicago Cubs, are secretly spending $'s against me. They better be careful, they have a lot to hide!'

Trump dismissed it as something ‘put out by Mitt Romney’s people’ referencing Katie Packer, who founded the super PAC and was Romney's deputy campaign manager in 2012

Tom Ricketts, Joe and Marlene's son, who owns the Chicago Cubs in his name, told reporters last week: 'It's a little surreal when Donald Trump threatens your mom.'

He said that his family aren’t afraid to make politic stands, adding they are ‘an open book’.

Ricketts added that he wasn’t afraid of Trump’s threats, saying: ‘If we had something to hide, you people would have found it by now.’

Speaking on Tuesday morning, Trump refused to take back any of the comments mentioned in the ad, insisting that he had not seen it.

‘I have not seen it so I would have to see it,’ he told Today’s Matt Lauer and Savannah Guthrie asked if he regretted any of the comments.

‘I have heard about the ad but I have not seen the ad.’

But earlier, when the hosts first mentioned the video, Trump dismissed it as something ‘put out by Mitt Romney’s people’.

He was referring to Katie Packer, who founded the Our Principles PAC in January this year in a bid to thwart Trump's presidential campaign. She served as Romney’s deputy campaign manager for his presidential run in 2012.

Trump said: ‘Mitt Romney is a sour guy.’

But Trump insisted he remains popular with women.

‘Every single poll of every single state that I’ve won of which I’ve won the vast majority, but every single poll coming out, the exit polls, I lead with women, I lead with the military, I lead with the vets, I lead with virtually everybody.’

Despite his claims, one female former employee often cited in Trump’s speeches as an example of how ‘great he is going to be for women’, says she ‘certainly would not vote for him for President’.

Barbara Res, who worked for the real estate mogul as the chief engineer of Trump towers in the 1980s, recently revealed she finds his policies ‘abhorrent’, citing examples such as ‘banning Muslims’ and his opposition to the Affordable Care Act.

DONALD TRUMP’S 'WAR ON WOMEN': HIS MOST DEROGATORY QUOTES The Republican frontrunner has launched many personal attacks on women, including Rosie O'Donnell (left) and Carly Fiorina (right) On Rosie O’Donnell, in a 2006 interview with Entertainment Tonight, Trump said: ‘Rosie O’Donnell’s disgusting, I mean both inside and out. ‘You take a look at her, she’s a slob. She talks like a truck driver. ‘I’d look her right in that fat, ugly face of hers, I’d say, “Rosie, you’re fired” from her television show The View.’ During a boardroom scene on a 2013 episode of Celebrity Apprentice, Trump was told that a female contestant had gotten down on her knees to beg. He responded with a double entendre, telling the contestant, model and actress Brande Roderick: ‘That must be a pretty picture, you dropping to your knees.’ In September last year, Rolling Stone published a profile on Trump, reporting how he blasted the appearance of his then-rival in the Republican presidential race. ‘Look at that face!’ he howled during a television interview. ‘Would anyone vote for that?’ ‘Can you imagine that, the face of our next president? I mean, she’s a woman, and I’m not s’posedta say bad things, but really, folks, come on. Are we serious?’ Shortly after Princess Diana’s death in 1997, Trump claimed he could have slept with her in an interview on The Howard Stern Show. ‘Why do people think it’s egotistical of you to say you could’ve gotten with Lady Di?’ Stern asked Trump. Trump replied: ‘I think I could have. She had times when she didn’t look great and she had times when she looked better than anyone in the world. ‘She had the height, she had this, she had magnificent skin, she was a great beauty.’ He added: ‘She was crazy, but these are minor details.’ After Fox News anchor Megyn Kelly grilled Trump on his history of insulting women during a televised debate, the billionaire launched a personal attack on her. In an interview with CNN, he said: 'I just don't respect her as a journalist. I have no respect for her, I don't think she's very good. She's highly overrated. 'I got out there they start saying all this stuff... she gets out and she starts asking me all sorts of ridiculous questions.’ Interpreted as an insulting reference to Kelly’s menstrual cycle, Trump said: ‘You could see there was blood coming out of her eyes, blood coming out of her wherever.' Advertisement

‘He started going around on all these stump speeches talking about how great he is going to be for women, and as an example, I hired a woman to be in charge of Trump Tower,’ she told the BBC.

She was hired by Trump in September 1980 - a time when female executives in the construction business were few and far between - to be in charge of building the 68-story Trump Tower on New York’s Fifth Avenue.

Res worked for him for 18 years, and she has said they had a ‘good’ relationship until they fell out after she wrote a book about her time working for him.

Despite this, Res said she does not have ‘an ax to grind’ against but added: ‘As far as being qualified, in my own opinion of him, I don’t think he has the temperament to be President.

‘I think he’s very impatient.’

Barbara Res, who was hired to work as chief engineer on Trump towers (pictured on a construction site) says she ‘certainly would not vote for him for President’

She added: ‘I certainly don't think Donald, with the way he carries on, and he's "kicking A" and "beating the S out of people"...

‘That's not presidential. That's a hooligan. That's a bully from the neighborhood. That's not who you want to be president of the United States.'

She also added that Trump never called her for permission before using her story in his campaign speeches.