a, Cohesin (Scc1), shugoshin (Sgo1) and condensin (Brn1) enrichment in metaphase-arrested cells in the presence of nocodazole in the pericentromeric region of wild-type and reoriented chromosome IV is shown (n = 2; immunoprecipitation was performed using proteins tagged with different epitopes in the two replicates). Asterisks indicate new peaks in reoriented strain. b, The distance between GFP foci does not change following gene reorientation. We measured 100 cells; horizontal lines indicate means. c, The region of separation upon gene reorientation does not extend beyond the next convergent gene pair. Strains with tetO arrays integrated at the indicated positions were arrested in metaphase, and the percentage of cells with two GFP foci was determined. For each biological replicate (data points; n = 3), 200 cells were scored; data are means ± s.e.m. d, Pile-up of cis contacts across all 16 pericentromeres in the reoriented strain, in the presence of spindle tension. e, log 2 ratio map of Hi-C signal in pericentromere IV in wild-type and reoriented strains (n = 1). f, Model for pericentromere expansion and disorganization in the absence of convergent genes. g, Diagram showing biorientation of sister kinetochores following spindle repolymerization. Cells carrying chromosomal GFP labels, Spc42–tdTomato and pMET-CDC20 were released from G1 phase into a metaphase arrest by depleting Cdc20 in the presence of nocodazole. Nocadazole was washed out while maintaining metaphase arrest and the percentage of cells with two GFP foci was scored over time. Source Data