Opponents of Bendigo's first mosque are hoping to present their case to the country's highest court in a last-ditch bid to stop the controversial project.

Bendigo residents led by local woman Julie Hoskin have waged a long legal battle against the mosque since it was first approved for construction by Bendigo City Council in 2014.

An artist's impression of the proposed Bendigo mosque.

Ms Hoskin and her supporters took the matter to the Court of Appeal, after losing their case in the Victorian Civil and Administrative Tribunal.

But the Court of Appeal threw out the case on December 16 last year.