One Nation is barely being held together, with two of the party's four senators in open warfare, bringing their relationship to the brink.

Key points: Rod Culleton and One Nation party leader Pauline Hanson appear to be at loggerheads

Rod Culleton and One Nation party leader Pauline Hanson appear to be at loggerheads Sources say Senator Culleton's chief of staff Margaret Menzel is blocking contact between the two

Sources say Senator Culleton's chief of staff Margaret Menzel is blocking contact between the two Ms Menzel denies the allegations, One Nation employees are divided about the issue

Senator Rod Culleton refuses to say if he is happy to remain with the party, and leader Pauline Hanson is insisting she wants it to stay together.

The West Australian senator said he has had very little contact with his leader in recent weeks, but One Nation sources say that is not from a lack of trying.

Sources loyal to Senator Hanson have told the ABC Senator Culleton's chief of staff Margaret Menzel is blocking contact between Senator Culleton and Senator Hanson.

"Every morning he's invited to Pauline Hanson's office, the same as every other senator," the source said.

According to those sources, Ms Menzel "stops" Senator Culleton from attending the meetings and "staff constantly all roll their eyes when she intervenes".

But in a rare interview, Ms Menzel told the ABC she has never told Senator Culleton not to meet with Senator Hanson.

"No and nor would I, nor would I for anybody," Ms Menzel said.

"It's his discretion who he meets with and I understand the responsibilities of the job I hold and the job he holds very, very well and I take it very seriously."

Ms Menzel said One Nation sources should put their name to their comments.

Pauline Hanson and Rod Culleton have been squaring off in the media. ( AAP )

"I will not be intimidated, I will not see the staff here intimidated and I will continue to support a man of great integrity as his chief of staff."

Ms Menzel has been embroiled in a public row with Senator Hanson's chief of staff James Ashby. Senator Hanson has confirmed Mr Ashby threw or "underhanded" his phone to Ms Menzel during a meeting.

Senator Hanson has said the incident was not serious, because it was a friendly toss, not a malicious hurl.

One Nation employees are privately making claims and counter-claims about communication between the two senators, but party sources insist Senator Culleton is welcome at One Nation.

"We all want to see Rod either stay in this place with his dignity or leave with his dignity."

Senator Culleton's election to the Parliament is being examined by the High Court because he had been convicted of an offence carrying a jail term of one year or more at the time of the July 2 poll.

That may make him ineligible to sit in the Upper House, even though the conviction has since been quashed.

He is also being investigated by Queensland Police about whether he attempted to threaten a judicial officer and pervert the course of justice, over a letter he sent to a Queensland Magistrate.

Hanson, Culleton square off in the media

The two senators are having a war of words through the media.

Last night, Senator Hanson asked Senator Culleton to attend a meeting in her office to discuss the Queensland Police investigation.

Senator Culleton did not show up, which led Senator Hanson to make an appeal through the ABC.

"Rod, excuse me, I'm party leader, I expect you to come to my office and it's about being a team player and that's all I expect," Senator Hanson said.

"I have the respect from my other senators and I expect the same from Rod Culleton under the banner of One Nation, Pauline Hanson's One Nation."

On RN, Senator Culleton responded by saying he respected Senator Hanson as the party's leader "but she's certainly not the boss of my office".

"I have urgent business that I had to adhere to yesterday, and it just wasn't convenient me to rush at short notice however the invitation."

Sorry, this video has expired 'Excuse me, I'm party leader': Pauline Hanson says Rod Culleton should have come to her office

Katter says he's not trying to poach Culleton

Some One Nation members also believe Senator Culleton is considering moving to Katter's Australian Party (KAP), but leader Bob Katter is repeatedly claiming innocence of attempts to poach him.

Sources point to a friendship between Ms Menzel and Mr Katter, but a spokeswoman for Mr Katter said he "fiercely believes Rod needs to stay loyal to Pauline Hanson".

"Bob has known the Menzel, Margaret and her husband, for a long time ... they are friends from north Queensland," the spokeswoman said.

"Margaret Menzel hasn't operated for KAP."

Ms Menzel said she was not interested in recruiting for KAP.

Senator Hanson's party also fractured after the 1998 Queensland election, where One Nation picked up 11 seats.

Three of the 11 Queensland MPs quit the party within eight months of being elected.

Three more quit later in the year and formed the City Country Alliance.