Study yields insights into how plant cells grow

March 10, 2015

The fine hairs on the underside of this sunflower are trichomes, single-celled structures that can shed light on how plant cells get their shape. (Purdue Agricultural Communication/Tom Campbell)

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WEST LAFAYETTE, Ind. - A study by Purdue University plant scientists and University of Nebraska-Lincoln engineers advances our understanding of how plants control their shape and development at the cellular level.

Their findings could help researchers engineer better cotton fibers, improve plant defense against insects, alter plant architecture and toughen root response to drought.

"This collaboration enabled us to learn more about what really controls plant cell shape in one year than we had in the previous 10," said Daniel Szymanski, Purdue professor of botany and plant pathology and leader of the research team. "The degree to which our discoveries linked interacting systems and provided clear explanations for cell shape control was great. The opportunities to apply this knowledge are limitless."

The team used a combination of experimental data from live plant cells and computational modeling to gain new insights into how plant cytoskeletons - intricate networks of protein fibers and tubes within cells - cooperate to produce complex cell shapes.

Unlike mammalian cells, plant cells are rigid, constrained by a thick wall of fibers that have the tensile strength of steel. Plant cells can also come in complicated shapes, such as the pointed, three-branched Arabidopsis trichomes that served as cell models in Szymanski's study.

Previous research had shown that two intracellular fiber systems control plant cell shape: the microtubule cytoskeleton and the actin cytoskeleton. A protein complex moves along the microtubules - tiny tubes in the cell - leaving behind fibers that are patterned into the cell wall. These fibers act like hoops around a barrel, holding the cell in a cylindrical shape as it expands. Actin, a type of protein, is also essential for plant shape and development, but its role has not been well understood.

Szymanski and his fellow researchers found that actin fibers run throughout the cell, forming a network of long-distance "roadways" along which additional materials for the cell wall are transported. A protein complex known as ARP2/3 controls the production and distribution of actin in the cell and directs traffic along these roadways from its position at the tip of the trichome. It also forms a meshwork of actin that can influence the behavior and organization of the rest of the cell.

"How this protein complex influences the actin network of the cell was a major discovery," Szymanski said. "If we can learn more about the interactions between the actin and microtubule systems, we could translate this knowledge into improved cotton fibers and trichome plant defense systems in crop species. Engineering these is low-hanging fruit."

Two-color live-cell imaging showed that traffic along the actin network flows in two directions - toward the tip and toward the base. New material is delivered via this network to maintain new wall synthesis during cell growth, though further research is needed to reveal what types of cell wall cargo are being delivered on the actin tracks, Szymanski said.

Nebraska-Lincoln engineers Joseph Turner and Anastasia Desyatova used Szymanski's cell measurements to design a computational model of a thin-walled pressurized cell that could be divided into sectors with different mechanical properties. This model is the first to include wall fibers and also reflected the tremendous internal pressure of a plant cell - 90 pounds per square inch, about the same pressure as a road-bike tire.

The model produced a number of predictions about cell wall properties that Szymanski then verified in live trichomes.

Actin allows the cell wall to maintain a thickness gradient - thicker at the base and thinner at the tip - as it grows. Actin also enables the cone-shaped tip of the cell to change in a fixed pattern, generating a highly specialized shape that is effective in insect defense.

Additionally, the team found that shape is set early in cell development and remains unchanged as the cell expands.

"The beauty of this modeling is that it can be applied to any plant cell," Szymanski said. "By combining biochemistry, genetics and cell biology, we can better understand how cell wall properties are determined. This could lead to breakthroughs in other cell types as well."

Understanding cell shape could also be used to model organ growth and development, he said.

"By controlling the geometry and growth rates of groups of cells, you could then scale this up to control the size and shape of an organ such as a leaf, which is crucial for plant productivity."

The study was published in Nature Plants on March 2 and is available at http://www.nature.com/articles/nplants201514.

The National Science Foundation provided funding for the research.

Writer: Natalie van Hoose, 765-496-2050, nvanhoos@purdue.edu

Source: Daniel Szymanski, 765-494-8092, dszyman@purdue.edu

ABSTRACT Patterning mechanisms of cytoskeletal and cell wall systems during leaf trichome morphogenesis Makoto Yanagisawa 1; Anastasia S. Desyatova 2; Samuel Belteton 1; Eileen L. Mallery 1; Joseph A. Turner 2; Daniel B. Szymanski 1,3 1 Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907, USA 2 Department of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska 68588, USA 3 Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA E-mail: dszyman@purdue.edu The plant actin cytoskeleton is an unstable network of filaments that influences polarized growth through poorly understood mechanisms. Here, we used a combination of live cell imaging and finite element computational modeling of Arabidopsis trichome morphogenesis to determine how the actin and microtubule cytoskeletons cooperate to pattern the cell wall and growth. The actin-related protein (ARP)2/3 complex generates an actin meshwork that operates within a tip-localized, microtubule-depleted zone. The actin meshwork also positions an actin bundle network that organizes organelle flow patterns. This activity is required to maintain cell wall thickness gradients that enable tip-biased diffuse growth. These newly discovered couplings between cytoskeletal patterns and wall textures provide important insights into the cellular mechanism of growth control in plants.

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