Mr. Tambor, who dedicated his best actor prize at the 2015 Golden Globes — among the first ever given for a transgender role — to the transgender community, said in his statement that he regretted “if any action of mine was ever misinterpreted by anyone as being aggressive,” but has denied the accusations.

Mr. Tambor, too, produced a supporting account. In a statement provided by the actor’s publicist, Allan Mayer, a hairstylist on the show, Terry Baliel, said that he had never witnessed the actor doing anything of an “inappropriate sexual” nature.

In his own statement, Mr. Tambor referred obliquely to his own sense of discomfort with what was happening on “Transparent,” saying that a “politicized atmosphere” had afflicted the set. “This is no longer the job I signed up for,” he said.

A few days later, in a new statement provided to The Times, Mr. Mayer expanded on Mr. Tambor’s position: “What he said was that given the toxic atmosphere and the politicization on the set, it’s very hard for him to see how he can possibly return. But no final decision for next year has been made, either by Jeffrey or by Amazon.” He declined to elaborate on what Mr. Tambor meant by toxic atmosphere and politicization of the set.

Responding to questions, Mx. Soloway wrote, “I take what Van Barnes and Trace Lysette are saying very seriously,” but declined to discuss the accusations, citing Amazon’s internal investigation.

“Transparent” may not have been the most popular series streaming on Amazon or its chief rival, Netflix. But it was among the most decorated, amassing awards and rapturous reviews and helping Amazon Studios burnish its reputation in the area of original content.