Given the importance of differentiating DIC from TTP and HUS, we propose a concise algorithm based on existing guidance [3, 9, 11] and our own discussions which will enable the intensivist to rapidly distinguish between these entities (Fig. 1). MAHA, negative Coombs test, elevated lactate dehydrogenase (LDH) levels, and organ dysfunction with thrombocytopenia are common to DIC, TTP, and aHUS [2, 3], although patients with TTP and septic DIC may have more severe thrombocytopenia [2, 12]. The most important distinguishing factor between DIC and TMAs is the coagulation profile, as patients with DIC have altered coagulation [2]. However, blood pressure is also important as HUS often presents with severe hypertension and DIC with hypotension [3, 7]. The combined evaluation of full blood count and blood smear, hemolysis profile, coagulation profile, and blood pressure is usually sufficient to ascertain whether a patient has DIC or a TMA.

Once DIC has been excluded, confirming the cause of the TMA is paramount for appropriate management. The two most concerning causes of TMA are TTP and HUS. TTP is caused by a deficiency in a disintegrin-like metalloproteinase with thrombospondin motif type 1 member 13 (ADAMTS13) and has 90% mortality without plasma exchange [7]. HUS is caused by either Shiga toxin (STEC-HUS) or complement dysregulation as a result of genetic predisposition or autoantibodies (aHUS) [3, 6, 7, 11]. An ADAMTS13 activity of < 5–10% is sufficient to confirm TTP [3, 9] and a positive Shiga-toxin stool culture or polymerase chain reaction (PCR) assay confirms STEC-HUS [3, 9]. In the absence of low ADAMTS13 levels and Shiga-toxin, aHUS, a rare but devastating TMA, is highly likely [6]. Similar to DIC, aHUS has a rapid onset and non-specific presentation [2, 3]. aHUS can be found in association with other complement-activating states such as infection, malignant hypertension, the post-partum period, kidney transplantation, certain drugs, or malignancies [3]. There can be substantial overlap in the presentation of these conditions and they may coexist with complement-mediated aHUS, making distinction difficult [3]. It should also be remembered that aHUS can present with malignant hypertension, which itself can cause TMA [6, 9]. Rapid diagnosis and treatment are essential to prevent irreversible organ damage and death [13].

Like any pragmatic guidelines, we chose to focus on the most common presentation as we considered this of most benefit. For comprehensive guidance on TMA diagnosis and management, we refer to other works, such as those of Scully et al. [7], Campistol et al. [3], Laurence et al. [9], and Azoulay et al. [11]. While the proposed algorithm applies to the majority of cases of thrombocytopenia, it must be noted that clinical judgment and collaboration with experts is essential, as exceptional clinical presentations do occur [14, 15].

It should also be noted that some of the tests required in the differential diagnosis (e.g., ADAMTS13 activity assay) are not available at all institutions. If rapid ADAMTS13 testing is not possible, the PLASMIC score, a seven-component prediction tool that can accurately and reliably predict the probability of severe ADAMTS13 deficiency [10], can be used. Additionally, we have not included genetic testing for the complement abnormalities of aHUS in our algorithm; while these can confirm an already suspected diagnosis of aHUS, the turnaround time is currently considerable and should not be relied upon in the ICU [11].

Critically ill patients have a range of clinical problems, including multi-organ failure, sepsis, and shock [5], and early diagnosis and management are crucial to optimize outcomes. We present a concise diagnostic algorithm that enables intensivists to make a rapid diagnosis so that they can initiate early appropriate management for ICU patients with thrombocytopenia. This algorithm adds to the current literature available to the intensivist [11], with a focus on differentiating TTP and HUS from DIC.