Got cavities? Blame Streptococcus mutans, a bacterium found in the mouth that produces acids strong enough to dissolve tooth enamel, allowing the microbe to bore into teeth and cause decay. Now, researchers report that some particularly pathogenic strains of S. mutans have extra arsenal at their disposal: a suite of antibacterial compounds that inhibit the growth of beneficial bacteria that help protect against dental cavities (ACS Infect. Dis. 2020, DOI: 10.1021/acsinfecdis.9b00365).

Microbiologist Anna Edlund of the J. Craig Venter Institute and her colleagues investigated a set of genes, known as the muc gene cluster, that is carried by some S. mutans strains, to see why they might be important to these pathogens. They found that muc is responsible for producing reutericyclin, an antibiotic also produced by bacteria that ferment sourdough, changing the microbial community within the bread.

Edlund, along with Bradley Moore of the University of California San Diego and their colleagues, studied the muc gene cluster in S. mutans taken from the mouth of a child with severe dental caries. When they introduced a mutation that knocked out one of the genes, mucD, the cells did not produce four metabolites made by the unmutated strain. The team identified them as reutericyclin, two of its analogs, and another derivative named mutanocyclin—all known antibacterial molecules.

To understand why S. mutans might produce these antibiotics, the researchers created mutants of the strain lacking different muc genes, and cultured them in the presence of six other common bacteria that are part of a healthy oral microbiome. They found that the pathogenic strain and its mutants strongly inhibited the beneficial species’ growth; the most effective inhibitor was a mutant that produced only reutericyclin.

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In contrast, a lab strain of S. mutans that lacked the muc gene cluster inhibited these other microbes much less, suggesting that the pathogenic strain—which causes much more severe disease—might use these molecules to remove the bacteria that block its growth.

Future studies will need to determine whether these secreted molecules play similar roles within the human mouth, not just in petri dishes, says microbiologist Jeffrey Banas of the University of Iowa, who was not involved with the work. The discovery is an “interesting variable” that could help explain differences between S. mutans strains that cause severe disease and those that are less harmful, he adds. “People have looked for strain differences for years” to figure out what sets the bad ones apart, Banas says. “This is the tip of the iceberg and is certainly worth pursuing. Acid production really allows these bacteria to gain a foothold, and then something—perhaps this antibiotic activity...helps it even further.”