President Trump Donald John TrumpSteele Dossier sub-source was subject of FBI counterintelligence probe Pelosi slams Trump executive order on pre-existing conditions: It 'isn't worth the paper it's signed on' Trump 'no longer angry' at Romney because of Supreme Court stance MORE on Wednesday called on Attorney General Jeff Sessions Jefferson (Jeff) Beauregard SessionsRoy Moore sues Alabama over COVID-19 restrictions GOP set to release controversial Biden report Trump's policies on refugees are as simple as ABCs MORE to stop the Russia investigation, a significant escalation of his attacks against the long-running probe that has dogged his presidency.

"Attorney General Jeff Sessions should stop this Rigged Witch Hunt right now, before it continues to stain our country any further," Trump tweeted.

The president also accused special counsel Robert Mueller Robert (Bob) MuellerCNN's Toobin warns McCabe is in 'perilous condition' with emboldened Trump CNN anchor rips Trump over Stone while evoking Clinton-Lynch tarmac meeting The Hill's 12:30 Report: New Hampshire fallout MORE of being "totally conflicted," adding that "his 17 Angry Democrats that are doing his dirty work are a disgrace to USA."

..This is a terrible situation and Attorney General Jeff Sessions should stop this Rigged Witch Hunt right now, before it continues to stain our country any further. Bob Mueller is totally conflicted, and his 17 Angry Democrats that are doing his dirty work are a disgrace to USA! — Donald J. Trump (@realDonaldTrump) August 1, 2018

Sessions has long been the target of Trump's ire over his decision to recuse himself from the Russia investigation, but the president has seldom suggested publicly that his attorney general should halt the probe.

Several Senate Republicans downplayed Trump's tweet, saying it is not Sessions’s decision to end the probe because of his recusal.

"The investigation will continue," said Sen. Cory Gardner Cory Scott GardnerBillionaire who donated to Trump in 2016 donates to Biden The Hill's Morning Report - Sponsored by Facebook - Trump previews SCOTUS nominee as 'totally brilliant' Cook Political Report shifts Colorado Senate race toward Democrat MORE (R-Colo.)

But others expressed alarm that the president would issue such a stark ultimatum to the Justice Department, which has traditionally strived to carry out criminal investigations free from political interference.

"I think it's highly inappropriate and intemperate," said Sen. Susan Collins Susan Margaret CollinsThe Hill's Campaign Report: Trump faces backlash after not committing to peaceful transition of power Billionaire who donated to Trump in 2016 donates to Biden Credit union group to spend million on Senate, House races MORE (R-Maine), who exited an elevator to answer reporters' questions about the tweet.

"It would be far better if the president just refrained from commenting and Mr. Mueller proceeds with his investigation, which after all has already resulted in more than 30 indictments ... and has led to a trial that is ongoing even as we speak," she added.

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White House press secretary Sarah Huckabee Sanders said Trump was not issuing a formal directive to Sessions.

"It's not an order. It's the president's opinion," Sanders told reporters during a press briefing.

Sanders called the special counsel's probe into ties between the Trump campaign and Russian interference in the 2016 "ridiculous" and said the president "wants to see it come to an end."

"The president is not obstructing. He is fighting back,” she added.

Trump's latest barb comes one day after his former campaign chairman, Paul Manafort Paul John ManafortOur Constitution is under attack by Attorney General William Barr Bannon trial date set in alleged border wall scam Conspicuous by their absence from the Republican Convention MORE, went on trial for alleged tax, financial and bank fraud crimes, the biggest test yet for the Mueller probe.

The president also sought to distance himself from Manafort early Wednesday, saying in another tweet that Manafort only "worked for me for a very short time" and suggested federal authorities should have informed him his campaign chief was under investigation during the 2016 presidential race.

"Paul Manafort worked for Ronald Reagan, Bob Dole and many other highly prominent and respected political leaders. He worked for me for a very short time. Why didn’t government tell me that he was under investigation. These old charges have nothing to do with Collusion — a Hoax!"

Paul Manafort worked for Ronald Reagan, Bob Dole and many other highly prominent and respected political leaders. He worked for me for a very short time. Why didn’t government tell me that he was under investigation. These old charges have nothing to do with Collusion - a Hoax! — Donald J. Trump (@realDonaldTrump) August 1, 2018

Trump later accused federal officials of treating Manafort worse than Mafia boss Al Capone, who served 11 years in prison after being convicted of tax evasion.

Looking back on history, who was treated worse, Alfonse Capone, legendary mob boss, killer and “Public Enemy Number One,” or Paul Manafort, political operative & Reagan/Dole darling, now serving solitary confinement - although convicted of nothing? Where is the Russian Collusion? — Donald J. Trump (@realDonaldTrump) August 1, 2018

Mueller, a Republican, has indicted or secured guilty pleas from 32 individuals, including four former Trump aides, in the probe into ties between the Trump campaign and Russia’s efforts to influence the 2016 presidential election.

Trump has repeatedly denied that his campaign was involved in the effort and has also wavered on whether Russia interfered in the election, as U.S. intelligence agencies have concluded.

For months, the president has kept up a sustained attack on the Mueller investigation in an attempt to undermine it in the eyes of the public.

Multiple media reports have said Trump privately pressured Sessions several times to reverse his decision to recuse himself. Those comments, plus his tweets attacking Sessions and former FBI Director James Comey James Brien ComeySteele Dossier sub-source was subject of FBI counterintelligence probe Judge will not dismiss McCabe's case against DOJ Democrats fear Russia interference could spoil bid to retake Senate MORE, are reportedly under investigation as Mueller looks into whether Trump has obstructed justice.

Sessions, the former Alabama senator who served as a top Trump campaign adviser, recused himself in March 2017 from the Russia probe after it was revealed he failed to disclose a conversation he had with Russia's U.S. ambassador during the 2016 race.

That decision left Deputy Attorney General Rod Rosenstein Rod RosensteinDOJ kept investigators from completing probe of Trump ties to Russia: report Five takeaways from final Senate Intel Russia report FBI officials hid copies of Russia probe documents fearing Trump interference: book MORE to oversee the investigation. Rosenstein appointed Mueller as special counsel in May 2017, after Trump fired Comey as FBI director.

Updated at 1:42 p.m.