Abstract Tree growth has been reported to increase in response to recent global climate change in controlled and semi-controlled experiments, but few studies have reported response of tree growth to increased temperature and atmospheric carbon dioxide (CO 2 ) concentration in natural environments. This study addresses how recent global climate change has affected height growth of trembling aspen (Populus tremuloides Michx) and black spruce (Picea mariana Mill B.S.) in their natural environments. We sampled 145 stands dominated by aspen and 82 dominated by spruce over the entire range of their distributions in British Columbia, Canada. These stands were established naturally after fire between the 19th and 20th centuries. Height growth was quantified as total heights of sampled dominant and co-dominant trees at breast-height age of 50 years. We assessed the relationships between 50-year height growth and environmental factors at both spatial and temporal scales. We also tested whether the tree growth associated with global climate change differed with spatial environment (latitude, longitude and elevation). As expected, height growth of both species was positively related to temperature variables at the regional scale and with soil moisture and nutrient availability at the local scale. While height growth of trembling aspen was not significantly related to any of the temporal variables we examined, that of black spruce increased significantly with stand establishment date, the anomaly of the average maximum summer temperature between May-August, and atmospheric CO 2 concentration, but not with the Palmer Drought Severity Index. Furthermore, the increase of spruce height growth associated with recent climate change was higher in the western than in eastern part of British Columbia. This study demonstrates that the response of height growth to recent climate change, i.e., increasing temperature and atmospheric CO 2 concentration, did not only differ with tree species, but also their growing spatial environment.

Citation: Messaoud Y, Chen HYH (2011) The Influence of Recent Climate Change on Tree Height Growth Differs with Species and Spatial Environment. PLoS ONE 6(2): e14691. https://doi.org/10.1371/journal.pone.0014691 Editor: Dorian Q. Fuller, University College London, United Kingdom Received: July 16, 2010; Accepted: January 25, 2011; Published: February 16, 2011 Copyright: © 2011 Messaoud, Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: Natural Sciences and Engineering Research Council of Canada (DG283336-09) and an Ontario Early Researcher Award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors have declared that no competing interests exist.

Introduction Since the beginning of the Industrial Revolution in 1750, atmospheric carbon dioxide (CO 2 ) concentration has increased steadily from 278 to 385 ppm. Global temperature has also increased by 0.6±0.2°C since the end of the Little Ice Age in 1880 [1], [2]. LaMarche et al. [3] were the first authors to report increase of tree growth in relation to atmospheric CO 2 fertilization. Since then, various greenhouse and open top chamber experiments have shown higher growth of tree seedlings or short-lived trees with increased CO 2 [4], [5]. These experiments have provided significant insights into the potential effects of global change, but the growing conditions and durations in controlled environments have led to concerns about their possible implications in the real world [6], [7]. In natural environments, tree growth has been shown to increase with recent atmospheric CO 2 increase [8]–[10], while others found that increased tree growth is linked to global warming [11]–[13] or a combined effect of several factors such as atmospheric CO 2 fertilization, global warming, and anthropogenic atmospheric nitrogen (N) deposition because of the inherent co-variation among these variables in natural environments [14]. One possible reason for these different findings may be the difference in tree growing environments, which has caused some tree species to become more sensitive to changes in their environment at their range limit [15], [16]. For example, several studies reported a greater response of tree growth related to climate change at the higher latitude or elevation compared with those located at their optimum range or at their lower latitude or elevation [17], [18]. To date, most studies on climate change effects on tree growth have taken place at a small spatial scale. Furthermore, potential different responses of individual species have largely been ignored in elevated CO 2 research [19]. In North America, trembling aspen (Populus tremuloides Michx) and black spruce (Picea mariana Mill.) are the most widely distributed broadleaf and conifer species, respectively [20]. Both trembling aspen and black spruce can colonize burned areas immediately after wildfire. Trembling aspen reproduces through root suckering, whereas black spruce reproduces mostly from seeds after fire due to its semi-serotinous cores. In British Columbia, aspen is the most widely distributed deciduous species, whereas black spruce is generally seen in northern parts of the province [21]. Both species have wide ecological amplitudes, occurring with a range of soil moisture regimes from very dry to very moist and nutrient regimes from very poor to very rich. The objective of this study was to examine the potential effects of recent global climate change and atmospheric CO 2 on growth of trembling aspen and black spruce in British Columbia. While we expected height growth for both species is highly related to spatial environment over a large geographic area as reported in many other large-scale studies of tree height growth [14], [22]–[24], we hypothesized that (1) growth of these two species increases with increasing temperature and atmospheric CO 2 concentration associated with recent climate change, and (2) growth response of the two species to temporal environment differs with spatial environment, more pronounced at more limiting temperature environments, e.g., high elevations or latitudes, because the effect of increased CO 2 concentration on tree growth may be better facilitated by increased temperatures in these temperature limiting environments. To quantify global climate change effects on tree growth, previous studies have mostly used radial measurements [e.g.,[9],[25]]. In this study, we use tree height of dominant and co-dominant trees as a measure of tree growth. In comparison with radial growth, height of dominant and co-dominant trees is more strongly influenced by climate and site characteristics[26], [27]. Furthermore, height growth of dominant and co-dominant trees in a forest stand, e.g., site index (defined as the height of dominant and co-dominant trees at a reference age, usually 50 years at breast height), is a strong predictor for forest stand productivity and is less affected by stand density [23], [28], [29].

Discussion Spatial environment At the spatial scale, height growth of both species responded positively to temperature related variables. This finding was consistent with height growth studies located in middle to high latitudes in North America [30], [31] or in Europe [32], [33]. Warmer temperature and longer growing season, associated with lower latitudes and elevations, promote photosynthesis and carbohydrate allocation to the stem [34]. The slower height growth of aspen in the northwestern part of the province, i.e., higher longitudes, may be a result of shorter growing season because of the frequent period of cloudiness in the western part of the province [35]. As expected, our analysis also showed that strong influences of local site condition, measured by slope position, soil moisture and nutrient regimes, is a strong predictor for tree height growth as found in other site quality studies [22], [36]. Both species responded weakly to precipitation. Aspen height growth had a marginally positive and negative relationship with mean annual and summer precipitation, respectively, whereas spruce height growth was not significantly related to either precipitation variable. The weak or absence of a relationship between height growth and precipitation appears to support previous findings that tree growth in northern climates is driven by temperature than water availability [37]. Since spruce is located in the northern part of the province, where water evaporation is low [21], and the species occurs mostly on sites with no or minimum soil moisture limitations in western North America [38], [39], additional water input may reduce growth. This is because in colder environments with low soil evaporation, water surplus increases the frequency of flooding, which reduces the rate of photosynthesis due to the stomata closure and reduction of root activity [40]. Multiple regression analysis further confirmed that precipitation has no effect on height growth of either species, suggesting that precipitation is not a limiting factor or any potential influences of precipitation on height growth is coupled with temperature related variables, i.e., MST for aspen and GDD for spruce (Table 2). Consistent with the common thought [20], [41], aspen, a fast growing species, appeared to be more sensitive to the spatial environmental change (climate and local site condition) than spruce, in terms of the magnitude of their responses to the temperature related variables. Alternatively, their responses reflect the geographic range associated climatic variations in the studied province, i.e., a bigger range sampled in independent variables increase the statistical strength of their association with the dependent variable. Temporal environment Atmospheric CO 2 concentration is often mentioned as being responsible for recent increase in tree growth [8], [9], [42], whereas others attribute tree growth increase to the effect of increased temperature during the growing season [12], [13]. Our study indicated that the recent global warming coupled with increased atmospheric CO 2 concentration, and to a lesser extent of nitrogen deposition as it is low compared with other regions [43], is accountable for increased height growth during the period of 160 years for spruce. However, neither recent climate change variables (TMAX, PDSI) nor atmospheric CO 2 had affected aspen height growth at the scale of the entire province. Can the different responses of the two species be a result of their genetic differences or different strategies to adapt or acclimate to the recent increase in temperature and CO 2 ? Potential mechanisms associated with the different responses among species have not been well understood since few studies have compared the responses of different plant species to climate warming and elevated CO 2 [19]. Alternative to the fact that their genetic differences have dictated their difference responses to climate change and elevated CO 2 , the different responses of the two species could be a result of the different geographic ranges studied. In British Columbia, spruce is a northern species, well adapted to cold climates, while aspen is one of the widest distributed species [21]. The climate change coupled with increased CO 2 may have a positive effect on tree growth in northern rather than southern environments [12], [13], [16]. The height growth response to climate change differed with geographic locations for spruce with a greater height growth response at the western than at the eastern parts of the province. In the western part, spruce is located in higher elevations with cooler summer temperatures and higher water availability compared with the western part [44]. The better growth response of spruce in the western part of our sample area appears to be a result of the interplay of increased temperature and CO 2 in the environment where growing season temperature is more limited than water availability. Conclusions This study attempted to relate tree height growth of trembling aspen and black spruce to the recent global climate change in their natural environments in entire British Columbia. As expected, height growth of both species was positively related to temperature variables at the regional scale and with soil moisture and nutrient availability at the local scale. While height growth of trembling aspen was not significantly related to any of the temporal variables we examined, that of black spruce increased significantly with temporal variables including stand establishment date, the anomaly of the average maximum summer temperature between May-August, and atmospheric CO 2 concentration, but not with the Palmer Drought Severity Index. As hypothesized, the increase in spruce height growth associated with recent climate change differed with its geographic location, i.e., a higher increase in the western than in eastern part of the study area. However, the higher increase in the western part was apparently a result of the interplay of increased temperature and CO 2 in the environment where growing season temperature is more limited than water availability. The mechanisms for the different responses to climate change of the two species are, however, not well understood.

Materials and Methods Study area This study was designed to capture the widest range of climate, soil moisture, and soil nutrient regimes in which the study species can naturally establish in the province (Table 1 and Fig. 1) [44]. The study area is located between 49°N and 60°N and 115°30′W and 134°W with elevation ranging from 340 to 1285 m above sea level in British Columbia, Canada. Wildfire is a common stand-replacing disturbance in the study area. Both species studied grow on a variety of soils, ranging from shallow and rocky to deep loamy sands and heavy clays; black spruce can also grow on organic soils. Depending on regional climate, aspen occurs in co-dominance with black spruce, balsam poplar (Populus balsamifera L.) white spruce (Picea glauca (Moench) Voss), hybrid spruce (Picea engelmannii Parry ex Engelm. x Picea glauca (Moench) Voss), subalpine fir (Abies lasiocarpa (hook.) nutt.), lodgepole pine (Pinus contorta Dougl. ex. Loud.), white birch (Betula papyrifera Marsh.), and Douglas-fir (Pseudotsuga menziesii var. glauca). Black spruce is commonly associated with white spruce, lodgepole pine and trembling aspen. Sampling design We sampled 145 and 82 stands for aspen and spruce, respectively. All sampled stands were naturally established after wildfire, unmanaged, and without a history of suppression or damage. These stands are of variable ages (Table 1), and as such, for a given period of growth, e.g., ages between 0 to 50 years, stands had experienced a wide range of historical growing conditions of atmospheric CO 2 and temperature associated with global climate change. Within each sampled stand, a plot of area 0.04 ha was established. Soil and vegetation were described according to Luttmerding et al. [45]. Topographic maps were used to determine the latitude and longitude of each plot, elevation was measured with a Thommen pocket altimeter, and aspect was measured with a Suunto pocket compass. Soil moisture (SMR) and nutrient regimes (SNR) were estimated using regional ecosystem field guides [46]. Within a plot, three or four trees of largest diameters with no visible growth abnormalities were sampled. For each tree selected, stem discs were cut at 30 cm above the root collar, and at 1-m intervals to the top of the tree. Stunted trees had stem discs cut at 30 cm above the root collar and at 50-cm intervals to the top of the tree [22]. In the laboratory, each disc was transversely cut with a sharp knife and zinc oxide powder was added to make the rings clearly visible. With a microscope, rings were counted in two directions until the same count was obtained. Particular attention was paid to abrupt changes in radial increment indicating possible suppression or damages. Raw stem analysis data were adjusted using Carmean's algorithm to calculate tree height corresponding to the age at each sectioned disc [47]. An average height growth curve was then computed from three sampled trees for each study plot using Richards' three-parameters equation [29], [48]. For each plot, site index at the reference age of 50 years at breast height was then calculated from the fitted equation. Spatial environment For each sample plot, spatial climate attributes were derived from its spatial coordinates, latitude, longitude, and elevation by using the ClimateBC [49], [50]. These data were estimated by extrapolation of climate data from the meteorological stations located in the region for the period 1971–2000. The climate attributes included sum of growing degree-days >5°C (GDD), mean annual temperature (MAT, °C), mean summer temperature (June-August, MST, °C), mean annual precipitation (MAP, mm), and mean summer precipitation (MSP, mm, i.e., total precipitation May to September inclusive). To examine the spatial climate influence on site index, the averages of the above climate attributes for the period of 1971–2000 were used. Local site conditions including aspect, SMR, and SNR have a profound influence on tree height growth [22]. For each sampled plot, aspect was classified as ridge, east (azimuth 45–135°), south (azimuth 135–225°), west (azimuth 229–315°), north (azimuth 315-45°), or flat; SMR as very dry, moderately dry, slightly dry, fresh, moist, or very moist, and SNR as very poor, poor, medium, rich, or very rich. Climate change and atmospheric CO 2 concentration data To assess the effects of climate change and CO 2 on site index, we used reconstructions of the anomaly of the average maximum summer temperature between May-August (TMAX) and the Palmer Drought Severity Index (PDSI) for western Canada, provided by the National Oceanic and Atmospheric Administration (NOAA; http://www.ncdc.noaa.gov/paleo/recons.html) as predictors. The TMAX records were derived from climate reconstruction in the Canadian Rockies (Alberta, 52° 15′N and 117° 15W) for the period of 950 AD-1994 [51] to represent the temperature condition for the whole study area. The reference period for the anomaly values in the climate reconstruction is 1900–1980. The PDSI value is based on the soil water budget model and indicates cumulative long-term dry (negative) and wet (positive) weather conditions for a period spanning from 750 to 1990 (http://www.ncdc.noaa.gov/paleo/pdsidata.html) [52], [53]. Since historical PDSI data were available in 2.5×2.5° latitude and longitude grids (Fig. 1), we chose the nearest PDSI for each sampled plot. Historical global atmospheric CO 2 concentration data were obtained from Antarctic ice cores and from Mauna Loa observatory (Hawaii, USA) covering 1006 AD-1978 AD (http://cdiac.esd.ornl.gov/trends/co2/lawdome.html) and 1958–2008 (http://www.esrl.noaa.gov/gmd/ccgg/trends), respectively. Since site index refers to the cumulative height growth above breast-height (1.3 m) for a period of 50 years, we used the average of temporal climate conditions and CO 2 concentration of the corresponding period of tree growth to examine temporal influence on site index. Statistical analyses We first tested the effect of the spatial variables on the site index; we then examined the effect of the temporal variables on residual site index, allowing for the control of the influence of spatial variables before including the temporal variables [54]. For each plot, spatial environment was represented by climate variables, i.e., GDD, MAT, MST, MAP, and MSP, and site variables, i.e., aspect, SMR, and SNR. General linear model was used to evaluate the effects of spatial variables on site index. Both complete and various manually selected stepwise models were evaluated to derive the final spatial models, in which all selected independent variables were significant at α = 0.05. Overall model significance and goodness-of-fit was judged using the Likelihood Ratio Statistic and assessing change in Akaike's Information Criterion (AIC) scores. A change in AIC of >2 is considered a substantial change in the descriptive ability between models [55]. Model goodness-of-fit was interpreted from R2 and by evaluating the effect-size measures of the predictor variables retained in the final models. The effect size of the predictor variables was calculated using Eta-squared, which represents the proportion of total sum of squares explained by each predictor variable in the model [56]. The effect of individual temporal variables (establishment date, TMAX, CO 2 , and PDSI) on residual site index was first examined by simple regression analysis. To achieve normality and homogeneity of variance, root square transformation of residual site index was applied by adding a positive integer, i.e., 9 for aspen and 5 for spruce as the smallest value of residual site index was −8.5 and −4.1 for aspen and spruce, respectively. To test the second hypothesis, we used multiple regressions to relate the transformed residual site index to establishment date (ED), which correlates strongly with other temporal variables (Table S1), and geographic locations (latitude, longitude and elevation), which correlate strongly with spatial climate variables (Table S2), by including interaction terms of ED × latitude, ED × longitude, and ED × elevation. ED was calculated as the mean of the establishment dates of the three sampled trees. The response of the residual site index to other temporal variables and their interactions with geographic locations were also examined, but resulted in much weaker relationships. Similar to the analysis for spatial environment, we evaluated both complete and stepwise models, and the final models were selected based on AIC and a significance level of α = 0.05 for the selected independent variables. All analyses were performed with SYSTAT 12 [57].

Supporting Information Table S1. Pearson's correlation between establishment date (ED, year), the anomaly of the average maximum summer temperature between May-August (TMAX), atmospheric CO 2 concentration (ppm), and the Palmer Drought Severity Index (PDSI). https://doi.org/10.1371/journal.pone.0014691.s001 (0.03 MB DOC) Table S2. Pearson's correlation between geographic locations (latitude, longitude and elevation) and spatial climate variables, sum of growing degree-days >5°C (GDD), mean annual temperature (MAT, °C), mean summer temperature (June-August, MST, °C), mean annual precipitation (MAP, mm), and mean summer precipitation (MSP, mm, i.e., total precipitation May to September inclusive). https://doi.org/10.1371/journal.pone.0014691.s002 (0.03 MB DOC)

Acknowledgments We thank D. New, D. Brisco, D. Affleck, J. Fons, P. Krestov, Y. Wang, and C. Chourmouzis for their field and laboratory assistance and Tomislav Sapic for ARCGIS maps. We also thank the two anonymous reviewers for their constructive reviews.

Author Contributions Conceived and designed the experiments: HYHC. Performed the experiments: YM HYHC. Analyzed the data: YM HYHC. Contributed reagents/materials/analysis tools: YM HYHC. Wrote the paper: YM HYHC.