A Melbourne man says he was kicked out of an Uber for kissing his male friend after heading home from a night out.

Drew Ezell said he and a friend left the Laird Hotel in Collingwood at about 1:00am on Monday to catch an Uber home.

But he said the driver made them get out of the car about 30 seconds into the journey.

"We were kissing and I heard the driver say something like 'That's really disgusting'," Mr Ezell said.

"I was kind of shocked and looked up and he was pulling over to the side of the road on Hoddle Street and just said, 'I'm not going to drive you guys because what you're doing is disgusting and I won't have that in my car at all'."

Mr Ezell said he reported the incident to Uber who he said responded to him with an email saying they would look into the matter.

He said the incident was particularly hurtful in the wake of the massacre of 49 people at a gay club in Orlando, Florida.

"Having that bigotry thrown in my face, it struck a really angry part of me," Mr Ezell said.

Mr Ezell said he thought about news reports quoting the Orlando shooter's father saying his son had recently seen two men kissing and was offended.

"That kind of phrase got stuck in my head and it made me really nervous and frightened," he said.

It is the second incident of its kind in Melbourne recently, with an Uber driver removed from the car booking service earlier this month for calling a lesbian couple "faggots".

The ABC has contacted Uber for a response.