(CNN) Hundreds of people again took to the streets of Venezuela's capital Thursday to demonstrate against President Nicolas Maduro, a day after three people were shot dead during protests in the South American nation riled by an economic crisis and political rifts.

For the second day, the opposition, which accuses Maduro of creating a dictatorship, asked marchers to meet at 26 points throughout Caracas and try to converge on the office of the government's top human rights official.

It wasn't clear whether these marchers would have any more success reaching the office than Wednesday's thousands of demonstrators, who were directed away from government buildings by security forces firing tear gas canisters.

Video from Agence France-Presse showed hundreds, some carrying Venezuelan flags, walking through the city streets early Thursday afternoon. In a scene similar to the day before, police in riot gear lobbed tear gas canisters in front of a line of protesters, scattering some.

Venezuelan police line up before clashing with opposition activists on Thursday, April 6.

Venezuelan police line up before clashing with opposition activists on Thursday, April 6.

Thousands of demonstrators protest against Maduro in Caracas on Saturday, April 8.

Thousands of demonstrators protest against Maduro in Caracas on Saturday, April 8.

Demonstrators help a journalist whose leg was injured while covering clashes in Caracas on April 10.

Demonstrators help a journalist whose leg was injured while covering clashes in Caracas on April 10.

Clouds of tear gas spread across a highway in Caracas during clashes on Monday, April 10.

Clouds of tear gas spread across a highway in Caracas during clashes on Monday, April 10.

A protester suffering from the effects of tear gas is carried away on Thursday, April 13.

A protester suffering from the effects of tear gas is carried away on Thursday, April 13.

Rescue workers tend to a demonstrator hit by a tear gas canister during anti-government protests on April 19.

Rescue workers tend to a demonstrator hit by a tear gas canister during anti-government protests on April 19.

Riot police clash with demonstrators while tear gas fills the air in Caracas on April 19.

Riot police clash with demonstrators while tear gas fills the air in Caracas on April 19.

A demonstrator stands in front of an armored vehicle during protests in Caracas on Wednesday, April 19.

A demonstrator stands in front of an armored vehicle during protests in Caracas on Wednesday, April 19.

A demonstrator catches fire during protests in Caracas on May 3. It happened as protesters clashed with police and the gas tank of a police motorcycle exploded. Other photos from the scene showed the man being attended for burns to his body.

A demonstrator catches fire during protests in Caracas on May 3. It happened as protesters clashed with police and the gas tank of a police motorcycle exploded. Other photos from the scene showed the man being attended for burns to his body.

An armored National Guard vehicle runs over a protester in Caracas on Wednesday, May 3. The protester, 22-year-old Pedro Michell Yaminne, survived, his mother told CNN. Interior and justice minister Nestor Reverol told reporters that the "lamentable" incident was under investigation. He said that moments before Yaminne was run over, demonstrators hurled a Molotov cocktail at the armored vehicle, opened the side door and "brutally assaulted" the driver.

Opposition activists clash with riot police in Caracas on Monday, May 8.

Opposition activists clash with riot police in Caracas on Monday, May 8.

Thousands of Venezuelan protesters march on a Caracas highway on Wednesday, May 10.

Thousands of Venezuelan protesters march on a Caracas highway on Wednesday, May 10.

Opposition activists scuffle with riot police in Caracas on May 12.

Opposition activists scuffle with riot police in Caracas on May 12.

During a "Grandparents' March" in Caracas, a man is blocked by police from reaching the Government Ombudsman's Office on Friday, May 12.

A street in eastern Caracas is painted with the names of people killed during more than two months of protests.

A street in eastern Caracas is painted with the names of people killed during more than two months of protests.

People flee during a clash between opposition demonstrators and riot police in Caracas on June 19.

People flee during a clash between opposition demonstrators and riot police in Caracas on June 19.

An opposition activist displays bullet shells during an anti-government demonstration on Monday, June 19.

An opposition activist displays bullet shells during an anti-government demonstration on Monday, June 19.

Maduro holds up a copy of the Venezuelan constitution during a news conference at the presidential palace in Caracas on June 22. Maduro has called for changes to the constitution amid the unrest.

Maduro holds up a copy of the Venezuelan constitution during a news conference at the presidential palace in Caracas on June 22. Maduro has called for changes to the constitution amid the unrest.

Protester David Jose Vallenilla is shot through a fence by a member of the National Guard near a military base in Caracas on Thursday, June 22. Vallenilla later died in the hospital after suffering three gunshot wounds to the chest.

Protester David Jose Vallenilla is shot through a fence by a member of the National Guard near a military base in Caracas on Thursday, June 22. Vallenilla later died in the hospital after suffering three gunshot wounds to the chest.

Protesters tear down a fence at the La Carlota airbase outside Caracas on Friday, June 23. A soldier shot a demonstrator through the fence at the base on the previous day, killing him.

National Guard members clash with deputies of the National Assembly in the Parliament's courtyard in Caracas on Tuesday, June 27.

National Guard members clash with deputies of the National Assembly in the Parliament's courtyard in Caracas on Tuesday, June 27.

Venezuelan lawmakers Luis Stefanelli, left, and Jose Regnault appear stunned in a corridor of the National Assembly after a clash with demonstrators in Caracas on Wednesday, July 5. Supporters of Maduro stormed the building and attacked opposition lawmakers, witnesses said. At least seven legislative employees and five lawmakers were injured, according to National Assembly President Julio Borges. Journalists said they were also assaulted.

Venezuelan police Officer Oscar Perez joins in an anti-government protest in Caracas on Thursday, July 13. Perez allegedly piloted a stolen police helicopter used to attack the Venezuelan Supreme Court with grenades on June 27.

A demonstrator dressed as Venezuelan independence hero Simon Bolivar is silhouetted against a national flag in Caracas on Monday, July 24.

A demonstrator dressed as Venezuelan independence hero Simon Bolivar is silhouetted against a national flag in Caracas on Monday, July 24.

A member of the National Guard fires at protesters during clashes in Caracas on Friday, July 28.

A member of the National Guard fires at protesters during clashes in Caracas on Friday, July 28.

A protester wounded by a pellet gun receives attention July 30 during a demonstration against the vote for a Constituent Assembly.

A protester wounded by a pellet gun receives attention July 30 during a demonstration against the vote for a Constituent Assembly.

A demonstrator shouts slogans through a traffic cone during an anti-government protest in Caracas on July 30.

A demonstrator shouts slogans through a traffic cone during an anti-government protest in Caracas on July 30.

A wounded anti-government demonstrator is helped by medics during clashes with police in Caracas on July 30.

A wounded anti-government demonstrator is helped by medics during clashes with police in Caracas on July 30.

Members of Venezuela's national police are caught in an explosion as they ride motorcycles near Altamira Square in Caracas on July 30. Venezuela has seen widespread unrest since March 29, when the Supreme Court dissolved Parliament and transferred all legislative powers to itself. The decision was later reversed, but protests have continued across the country, which is also in the midst of an economic crisis.

Maduro celebrates the results of a national vote on Sunday, July 30. His opponents boycotted the election and demonstrated against it for weeks, saying he orchestrated it to get around the existing National Assembly, which the opposition has controlled since 2015. Maduro has argued that the Constituent Assembly will help bring peace to a polarized country, with all branches of the government falling under the political movement founded by his late mentor and predecessor, Hugo Chavez.

Maduro celebrates the results of a national vote on Sunday, July 30. His opponents boycotted the election and demonstrated against it for weeks, saying he orchestrated it to get around the existing National Assembly, which the opposition has controlled since 2015. Maduro has argued that the Constituent Assembly will help bring peace to a polarized country, with all branches of the government falling under the political movement founded by his late mentor and predecessor, Hugo Chavez.

Opposition lawmaker Juan Requesens addresses a rally in Caracas on July 31. Two other leading opposition figures, Leopoldo Lopez and Antonio Ledezma, were rounded up from their homes, according to their families.

A woman in Caracas attends a vigil Monday, July 31, for anti-government activists who have died in the country's recent unrest. More than 120 people have been killed in Venezuela since early April, according to the attorney general's office.

Diosdado Cabello, a member of Venezuela's National Constituent Assembly, holds an image of Venezuela's late President Hugo Chavez as delegates gather for a group photo following their swearing-in ceremony on Friday, August 4.

Diosdado Cabello, a member of Venezuela's National Constituent Assembly, holds an image of Venezuela's late President Hugo Chavez as delegates gather for a group photo following their swearing-in ceremony on Friday, August 4.

Venezuela's Chief Prosecutor Luisa Ortega Diaz, third from left, is surrounded by employees of the General Prosecutor's office as she is barred by security forces from entering her office in Caracas on Saturday, August 5.

Members of the Venezuelan armed forces fly over Valencia in a helicopter while citizens demonstrate in support of a group that staged a paramilitary uprising at the Paramacay military base on August 6.

Members of the Venezuelan armed forces fly over Valencia in a helicopter while citizens demonstrate in support of a group that staged a paramilitary uprising at the Paramacay military base on August 6.

A man is arrested in Valencia during clashes between anti-government activists and the National Guard on August 6.

A man is arrested in Valencia during clashes between anti-government activists and the National Guard on August 6.

Soldiers stop a vehicle and detain its passengers on the Paramacay military base in Valencia, Venezuela, on August 6. According to authorities, two people were killed when an anti-government paramilitary attack was quelled at the base.

A costumed demonstrator in Caracas, Venezuela, protests the government of Venezuelan President Nicolas Maduro on Sunday, August 6. Unrest continued to sweep through the country after a new legislative assembly was inaugurated following a controversial election on July 30. The new assembly has wide-ranging powers and is expected to rewrite the Venezuelan constitution at Maduro's behest. Opposition supporters, who see the vote as a power grab and an erosion of democracy, boycotted and staged demonstrations against the vote.

At least three people were killed in shootings during Wednesday's demonstrations across the country , officials and relatives said, bringing the death toll in protests since March 29 to at least nine.

Venezuela has been rocked by intermittent but violent protests since the Supreme Court dissolved an opposition-led parliament last month. The move was reversed days later, but demonstrations had already erupted.

Further fueling the protests: The government banned opposition leader Henrique Capriles from all political activity for 15 years on April 7.

The turmoil is set against an economic crisis in which unemployment is set to surpass 25%, and people have struggled for years with food and medical shortages and skyrocketing prices.

Opposition leaders are calling for the National Assembly's powers to be fully restored, for all political prisoners to be freed, for a humanitarian corridor to be opened and for stalled elections to take place.

The country's economic crunch took another turn this week as the government seized a General Motors plant , prompting the auto giant to say it was halting operations in Venezuela.

Thursday's turnout could be lower than Wednesday's, in part because the latter was a holiday commemorating Venezuela's struggle for independence from Spain, journalist Stefano Pozzebon told CNN from Caracas.

Shooting deaths

A look at Wednesday's shooting deaths:

• A 17-year-old boy was shot in the head in Caracas, and Venezuela's public ministry said it is investigating the incident. The teenager, later identified as Carlos Moreno, died while undergoing surgery, a hospital representative said.

Video posted on social media showed a young man on the ground -- a pool of blood near his head -- surrounded by marchers in the San Bernardino neighborhood. A woman is heard yelling, "They've killed him."

Moreno's sister Alejandra said he did not join the marches but was on his way to play soccer.

• Paola Andreina Ramírez Gómez, 23, was shot in plaza San Carlos in San Cristóbal, the ministry said.

Jorban Contreras, a paramedic and director of the civil protection unit in Tachira state, said the woman already was dead from a gunshot wound to her chest when he arrived.

• Venezuelan National Guard Sgt. Niumar Jose San Clemente Barrios was fatally shot Wednesday night, said the public prosecutor's office and Venezuela's top human rights official, ombudsman Tarek William Saab. A second guardsman was wounded by a bullet; both were shot during "violent protests" in Los Salias, south of Caracas, according to Saab.

Maduro: Don't complain when law comes after you

Maduro deployed the Venezuelan armed forces to the streets on Sunday night amid rising tensions.

JUST WATCHED Venezuela turmoil escalates Replay More Videos ... MUST WATCH Venezuela turmoil escalates 01:33

He gave a fiery speech Wednesday, accusing opposition leaders of inciting violence. He called out the president of the opposition-controlled National Assembly, Julio Borges, saying, "You are the head of the coup. Later, don't complain when the law comes after you."

This week's marches underscored the widening political rifts in the country. The government has repeatedly blocked any attempts by the opposition to oust Maduro by a referendum vote. It has also delayed local and state elections.

Thousands rally Wednesday against Venezuelan President Nicolas Maduro in eastern Caracas.

The last vote held in Venezuela, the parliamentary election of 2015, gave the opposition a majority. Critics say elections have been delayed because Maduro is afraid of the outcome.

Maduro, 54, has been defiant, taking a confrontational tone with members of the opposition and protesters, whom he calls "vandals and terrorists."

Government supporters and security forces succeeded Wednesday in blocking marchers from reaching certain parts of the city, according to observers. Water cannons and tear gas canisters were unleashed on opposition marchers.

Video posted on social media showed marchers -- some covering their faces -- crossing the narrow Guaire River in the capital trying to elude tear gas blasts.

1:06 PM Represión brutal y cobarde en la Autopista Francisco Fajardo. Estamos en el Río Guaire resistiendo pic.twitter.com/wETbeCu2xh — David Smolansky (@dsmolansky) April 19, 2017

The video was posted by David Smolansky, mayor of the municipality of El Hatillo and an opposition leader.

3:20 PM Así se resiste en pleno Guaire ante la represión cobarde de la dictadura. Los venezolanos salimos pacíficamente por nuestra libertad pic.twitter.com/5K70OjI70a — David Smolansky (@dsmolansky) April 19, 2017

In Washington, US Secretary of State Rex Tillerson expressed concerns to reporters Wednesday that the "government of Maduro is violating its own constitution and is not allowing the opposition to have their voices heard, nor allowing them to organize in a way that expresses the views of the Venezuelan people."