Music constitutes an integral part of everyday life. There is great variation in preference patterns for music. However, the cause of such individual differences has not been fully elucidated to date. Many behavioral traits, including personality, are known to be influenced by steroid-hormone testosterone. On this basis, we conjectured that testosterone partly determines individual differences in music preference. To examine this hypothesis, in the present study, we investigated the association between salivary testosterone concentration and strength of preference for five different music types in young males and females. The results revealed a significant negative correlation between salivary testosterone concentration and preference for sophisticated music, such as classical and jazz in males. This relationship was not mediated by the big-five personality traits. These findings indicate the possibility that neuroendocrinological function can exert influences on music preference patterns.