Thousands of cyclists using St Kilda and Sydney roads each day will miss out on a $100 million taxpayer fund for better cycling routes.

Weeks before the 2014 election, Labor unveiled a $100 million Safer Cyclist and Pedestrian Fund to fill the "missing links" in the bicycle network as well as improve pedestrian paths.

The idea was to improve cycling routes to train stations, the CBD and boost cycling in the regions.

After Labor's win, Roads Minister Luke Donnellan went on a $58,000 bike tour of the Netherlands in 2015 to learn how to "support the significant growth in the use of bikes as a key mode of transport".

The government also unveiled a new bureaucratic arm within Transport for Victoria to focus exclusively on walking and cycling.