About 1,000 people from 30 action groups have joined forces and protested through Sydney CBD calling for the Government to fix NSW roads and transport.

United in their anger, unions and local community groups mobilised to protest against ongoing problems with the rail system, the privatisation of buses and the Government's major projects like the Sydney Metro, WestConnex, the F6 toll road and the Western Harbour Tunnel.

As the rally wound its way through the streets and entered Martin Place, protestors were asked to bunch up, just as they would on an overcrowded train.

Mary Court, secretary of Penrith Valley Community Unions, said private toll road building is "sucking billions" out of public transport and warned Premier Gladys Berejiklian not to "mess with the west".

"Shame, Gladys, shame," she said.

Protesters show they are less than impressed with Premier Gladys Berejiklian. ( ABC News: Emily Laurence )

Ms Court attacked the new M4 road toll, calling it "highway robbery".

"The M4 was already paid off in 2014," she said.

"Why are western Sydney and regional NSW being exploited to pay a massive tax for the next 41 years? To pay for WestConnex and the Harbour Tunnel.

"Dump this M4 toll or we will dump you."

The controversial WestConnex motorway is the biggest infrastructure project in Sydney since the Sydney Harbour Bridge.

While supporters of the motorway say it will slash commute times for those living in Sydney's west, there is also fierce opposition to spending money on a project that encourages driving over public transport.

Rozelle resident Ryan Gorman said WestConnex will "destroy our city".

"Public transport has got to be the answer for this city.

"Every other city in the world is getting ready of gigantic roads like WestConnex.

"And I think $17 billion could be spend a lot more wisely."

Endangered species v saving 5 minutes

Critics of the extension of the F6 freeway south of Sydney, which may see the clearing of 60 hectares of land in the Royal National Park, are concerned for endangered species that rely on sensitive wetlands.

"It's incredible to think the NSW Government would put such ecological communities at risk for five minutes of travel time savings," said Greta Werner from the F6 action group.

"We are [also] concerned tunnelling works are going to do damage to homes… and unfiltered exhaust stacks [will spew] matter over homes, schools and child care centre."

There is concern over toxic fumes which will come out of exhaust stacks from the F6 tunnelling. ( ABC News: Emily Laurence )

Late last year, the Government committed to the first stage of the project between Arncliffe and Kogarah.

The 4-kilometre tunnel is intended to reduce traffic congestion along the Princes Highway.

"We know that public transport is much more efficient but the Government is refusing to consider alternatives for our region," Ms Werner said.

"It is a 1950s-style solution that is projected to become ineffective in less time than it would take to build."

However, Ms Berejiklian said she is proud of the way her Government is building major transport projects in Sydney.

"We know that people want to enjoy time with their families, or get to work on time without having to be worried about being stuck in traffic," she said.