CREAM (Cosmic Ray Energetics and Mass): Last night the CREAM external payload was removed from the SpX-12 trunk by the Space Station Remote Manipulator System (SSRMS) and handed off to the Japanese Experiment Module Remote Manipulator System (JEMRMS), which then installed it on Exposed Facility Unit-2 (EFU-2) on the JEM Exposed Facility (JEF). Ground controllers then commanded activation of the CREAM payload. CREAM’s instruments measure the charges of cosmic rays over a broad energy range for a planned three years of operation on the ISS. The origins of cosmic rays and the mechanisms that accelerate them to high speeds are among the oldest questions in modern astrophysics. Results from CREAM are expected to bring the science community closer to answering those questions and build a stronger understanding of the fundamental structure of the universe.

Cardio Ox Ultrasound: A 50S crewmember completed their Return minus 15 Day (R-15) ultrasound, blood pressure and Electrocardiogram (ECG) measurements for the Cardio Ox investigation. Another crewmember provided scanning assistance after the subject crewmember donned the ECG electrodes and marked the carotid and brachial arteries for scanning. By collecting ultrasound and ECG data, along with blood and urine samples, scientists are trying to determine whether biological markers of oxidative and inflammatory stress are elevated during and after space flight and whether this results in an increased, long-term risk of atherosclerosis in astronauts.

ADvanced Space Experiment Processor (ADSEP): The crew removed Cell Culturing (CellCult) cassettes from ADSEP, inserted cells into each cassette and reinserted them into ADSEP. ADSEP is a thermally controlled facility that accommodates up to three cassette-based experiments that can be independently operated. Its companion hardware consists of a collection of several experiment cassettes that accommodate experiments in cell technology, model organisms, multiphase fluids, solution chemistry, separation science, microencapsulation, and crystal growth. For CellCult investigations, each cassette contains a rotating filtered bioreactor, a reservoir for fresh media, two peristaltic pumps, a waste reservoir, and up to 6 sample-collection or reagent containers connected by a manifold to the reactor. Cultures can be operated in continuous perfusion, batch fed, static, or sampling mode. The removal of samples and the addition of additives to the reactor volume can be programmed or teleoperated.

Multi-Omics-Mouse: The crew performed routine maintenance for the Multi-Omics-Mouse Cage Units, exchanging waste collection equipment and odor filters. Several studies have reported space flight effects on the human immune system, but the relationship between microbiota and immune dysfunction during flight remains unclear. In the Multi-Omics-Mouse investigation, food with and without fructooligosaccharides (FOS) will be used as prebiotics, to determine if they improve the gut environment and immune function. After the flight, researchers will analyze the gut environment (microbiota and metabolites) and immune system of the mice by multi-omics analysis.

Bigelow Expandable Activity Module (BEAM): The crew ingressed BEAM and collecteded air and surface microbial samples. BEAM is an experimental expandable module attached to the ISS. Expandable modules weigh less and take up less room on a rocket than a traditional module, while providing additional space on-orbit for living and working. Crews traveling to the moon, Mars, asteroids, or other destinations may be able to use them as habitable structures.

Treadmill 2 (T2): This afternoon the crew reported a clicking noise emanating from T2 that had not been heard previously. The crew took video and audio which were downlinked for ground team evaluation. T2 is currently no-go for exercise.

Today’s Planned Activities

All activities were completed unless otherwise noted.

Advanced Space Experiment Processor Cell Collection On-Board Training

Advanced Space Experiment Processor Cell Collection

ADSEP MELFI Sample Insertion #1

ADSEP MELFI Removal #2

AQUAPAD Training

KUBIK Functional check

BEAM Cleanup and Egress

BEAM Ingress

Bigelow Expandable Activity Module (BEAM) Node 3 Aft Stow/Unstow

Cardio Ox CDL Holter Arterial BP Hardware Doff – Subject

Cardio Ox CDL Holter Arterial BP Measurement – Subject

Cardio Ox Ultrasound Preparation

Cardio Ox Ultrasound Scan – Operator

Cardio Ox Ultrasound Scan – Subject

Combustion Integrated Rack Alignment Guide Install

ARED Platform Fold

ARED Platform Unfold Back to Nominal Position

Crew Departure Preparations for Return to Earth

Environmental Health System (EHS) – Microbial Air Sampler (MAS) Kit Sample Collection

Environmental Health System (EHS) – Surface Sample Kit (SSK) Collection/Incubation

Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis

Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record

Extravehicular Mobility Unit (EMU) Post Scrub Cooling Loop Water (H2O) Sample

Stow Syringes used in H2O Conductivity Test

Extravehicular Mobility Unit (EMU) Conductivity Test

EVA Extravehicular Mobility Unit (EMU) Cooling Loop Scrub Deconfiguration

Extravehicular Mobility Unit (EMU) Cooling Loop Maintenance Scrub Initiation

Extravehicular Mobility Unit (EMU) Cooling Loop Maintenance Iodination

Handover Feedback Questions

Health Maintenance System (HMS) ISS Medical Accessories Kit (IMAK) Unpack from SPX-12

Health Maintenance System (HMS) Tonometry Test Setup/Stow

Health Maintenance System (HMS) Tonometry Test Crew Medical Officer (CMO)

Health Maintenance System (HMS) Tonometry Test Subject

Health Maintenance System (HMS) Vision Questionnaire

Health Maintenance System (HMS) Vision Test

IFUNGUS sample MELFI Insertion #1

Auxiliary Computer System [ВКС] Hardware Audit

Lighting Effects Sleep Log Entry – Subject

Multi-Omics-Mouse Cage Cleaning Familiarization Conference

Multi-Omics-Mouse Cage Cleaning and Maintenance for Food 1G first part 1

Multi-Omics-Mouse Cage Cleaning and Maintenance for Food 1G first part 2

Multi-Omics-Mouse Item Gathering

Photo/TV N3/BEAM Camcorder Setup

Probiotics Fecal Sample MELFI Insertion

Probiotics Fecal Sample Operations

Probiotics Sample Photo

Reminder Rodent Research Thermal Consideration

Treadmill 2 (T2) Photo/TV Reminder

Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill

Наклейка русской клавиатуры на лэптоп RSК2

Pre-pack cargo items for return or disposal on Soyuz 735

Surface Tension Containment MWA Assist

Surface Tension Containment MWA Operations

Surface Tension Containment MWA Preperation

Ultrasound 2 HRF Rack 1 Power On

Water Recovery System Waste Water Tank Drain Init

Water Recovery System Waste Water Tank Drain Termination

XF305 Camcorder Setup

Test video recording for RT channel

Stop discharging the 1st Orlan Battery Pack 825М3 and and start discharging the 2nd Battery Pack

EVA tool stow, update IMS

RSK1 Laptop Replacement

DUBRAVA. Observation and photography using Photo Equipment

Virus Definition File Update on Auxiliary Computer System (ВКС) Laptops

Reconnecting SM KURS-P cables from to DC1 port Antenna Feeder to MRM2 port antenna feeder, Handover

Reconnecting SM KURS-P cables from to DC1 port Antenna Feeder DC1 to MRM2 port antenna feeder

МО-8. Configuration Setup

Body Mass Measurement – BMMD

МО-8. Closeout Ops

Hematocrit. Hardware Setup

LBNP Training (PRELIMINARY) Operator

Preparation of Reports for Roscosmos web site and social media

БРП-М water sampling to drink bags

СВО-ЗВ water sampling to Russian drink bags

Collecting condensate water samples [КАВ] up to [СРВ-К2М] БКО, equipment setup, sampler installation

Sampling atmospheric condensate [КАВ] upstream of БКО [СРВК-2М], Sampler Removal, Deconfiguration

Collecting condensate water samples [КАВ] to [СРВ-К2М] БКО, sampler replacement

Measuring Partial CO Pressure in RS using US portable CSA-CP device

Sampling condensate water [КАВ] upstream of СРВ-К2М Gas-liquid mixture filter (ФГС) to drink bags, end

Hardware prepack for return and disposal on Soyuz 735

URAGAN. Observation and Photography

ECON-M. Observation and photography

Completed Task List Activities

SABL2 Door Open

Ground Activities

All activities were completed unless otherwise noted.

CREAM install

EVA loop scrub

BEAM ingress

Three-Day Look Ahead:

Wednesday, 08/23: OBT Checs drill, mouse cage cleaning, Genes In Space surface sampling, crew departure prep

Thursday, 08/24: STaARS facility/controller/Nexus Lab install, Lung media fixation, ADSEP cell collection, THC/IMC flow measure

Friday, 08/25: Genes In Space, RR habitat restock, 50S prepack, WHC maintenance, Dragon transfer ops

QUICK ISS Status – Environmental Control Group: