"We find that the identified patterns are not present on other flows, and almost the entire price impact can be attributed to this one large player," Griffin and Shams wrote. "We map this data across both blockchains and find that the one player or entity (labeled as 1LSg throughout the paper) is behind the majority of the patterns we document."

Finance professors John Griffin and Amin Shams – instructors at University of Texas and the Ohio State University, respectively – analyzed over 200 gigabytes of data for the transaction history between bitcoin and tether, another digital currency. Tether is an asset known as a "stablecoin," which has its trading value connected to the dollar.

A forensic study on bitcoin's 2017 boom has found that nearly the entire rise of the digital currency at the time is attributable to "one large player," although the market manipulator remains unidentified.

Griffin and Shams were able to follow the clusters of data to a source: "One large account at Bitfinex." The digital currency exchange Bitfinex is one of the largest in the world. The study found that, through Bitfinex, the single player was able to manipulate demand for bitcoin via "extreme" flows of tethers. The Wall Street Journal first reported on the updated study's results on Monday.

The manipulation occurred as bitcoin rose to an all-time high of nearly $20,000 in late 2017, the study found. Bitcoin traded at about $9,300 on Monday.

"One of the SEC's top worries is that crypto is subject to manipulation. This study appears to lend credibility to that argument," Cowen analyst Jaret Seiberg said in a note on Monday.

The study comes after an analysis published in March found that 95% bitcoin spot trading is faked. The survey, created by cryptocurrency asset manager Bitwise for the SEC, found that only $273 million of about $6 billion in average daily bitcoin volume was legitimate.

Cowen said Griffin and Shams' study will likely add even more scrutiny of bitcoin and cryptocurrency at large, especially from regulators and lawmakers.

"We see this as further souring Washington on crypto and believe it is negative for efforts to launch crypto ETFs and for Facebook to launch Libra," Seiberg added.

Libra is Facebook's cryptocurrency project, which has seen several major backers drop out in the past month.

While the latest study doesn't identify the manipulator, the professors suggest those running Bitfinex either knew of the operation or were even possibly assisting the scheme. Bitfinex's general counsel Stuart Hoegner told the WSJ that the study "lacks academic rigor," saying that "it is the global rise of digital currency that has driven the market's demand for tether."

Both Bitfinex and Tether Ltd., the company that controls tether, are owned and operated by the same people. The WSJ noted that both companies are under investigations for alleged fraud by the Department of Justice and the New York Attorney General.

– CNBC's Tom Franck contributed to this report.