Critics are branding Stephen Colbert a homophobe over a joke he made about President Trump on Monday night's Late Show.

The episode, filmed at the end of President Trump's first 100 days in office, ended in a long-winded attack against the commander-in-chief - who he called a 'pricktator' who attracts 'more skinheads than free Rogaine'.

What critics took issue with was Colbert's comments about President Trump and Russian President Vladimir Putin.

Critics have called Stephen Colbert a homophobe after he made a racy joke about President Trump on the Late Show Monday night (above)

Colbert said the only thing Trump's 'mouth is good for is being Vladimir Putin's c**k holster'

'You talk like a sign language gorilla who got hit in the head. In fact, the only thing your mouth is good for is being Vladimir Putin's c**k holster,' Colbert said.

At the mention of the term 'c**k holster', the entire audience - which had been laughing through the more than 12-minute monologue - erupted into gasps.

The reaction was so loud that Colbert had to wait for them to settle back down to finish the rest of his speech.

The reaction online was just as pointed. Immediately, Trump supporters started branding Colbert a homophobe and called for CBS to fire him.

Others supported Colbert, expressing their feeling that the late night host did not intend to offend the gay community.

Trump supporters and members of the LGBTQ community alike condemned Colbert's comment

MSNBC journalist Lawrence O'Donnell was impressed by Colbert's monologue on Monday

Ever since Trump took office, Colbert's once flailing show has taken the lead in the late night ratings thanks to his pointed criticism of the president, which the previous ratings leader, Jimmy Fallon, has failed to muster.

After taping the show Monday afternoon, Colbert put on a tux and took his wife to the Met Gala

While Colbert has remained the leader for total viewers since the second week of Trump's administration, he still lags behind Fallon in the key 18-49 age bracket.

It's unclear how Colbert's latest comments will impact his ratings. Ratings are usually released the Tuesday as the previous week.

Colbert also has one of the stronger bookings this week with scheduled appearances by Amy Schumer, Jim Parsons, Charles Barkley and Richard Gere. Chris Rock, Chris Pine, Paul Giamatti and Gisele Bundchen are stopping by Fallon's show.

Colbert's other competitor in the 11:35pm time bracket is Jimmy Kimmel Live. Kimmel will be taking the rest of the week off for paternity leave, since his newborn son recently had open heart surgery.

Kimmel detailed his family's heartbreak in a tearful monologue Monday, while Colbert was taking down Trump.

Will Arnett, Anthony Anderson, Kirsten Bell and David Spade will be filling in for Kimmel while he is out.