bph15020-sup-0001-Figure_S1.jpgJPEG image, 676.3 KB

Figure S1. CHO cells viability measured by MTS assay and 2′,7′‐Dichlorofluorescein biochemical assay of the generation of ROS after 24 hours incubation. (A) Effect of gradual increasing of glucose concentration (10, 25, 50, 100, 150 mM) or mannitol (100 mM) on the cell viability of mock transfected or Na v 1.5 stably transfected cells. (B) Effect of gradual increasing of glucose concentration (10, 25, 50, 100, 150 mM) or mannitol (100 mM) on ROS production of mock transfected or Na v 1.5 stably transfected cells. (C) Effect of co‐incubation of CBD (1 or 5 μM), lidocaine (100 μM or 1 mM) or Tempol (100 μM or 1 mM) or their vehicle on the cell viability of Na v 1.5 transfected cells incubated in high glucose concentrations (100 mM). (D) Effect of co‐incubation of CBD (1 or 5 μM), lidocaine (100 μM or 1 mM) or Tempol (100 μM or 1 mM) or their vehicle on ROS production of Na v 1.5 transfected cells incubated in high glucose concentrations (100 mM). (E) Effect of co‐incubation of CBD (5 μM) or its vehicle on cell viability of untransfected cells incubated in normal (10 mM) or high glucose concentrations (100 mM). (F) Effect of co‐incubation of CBD (5 μM) or its vehicle on ROS production of untransfected cells incubated in normal (10 mM) or high glucose concentrations (100 mM). Values are expressed as means ± SEM of five independent experiments. In each experiment, each sample was repeated five times. *P < 0.05 versus corresponding “Control” values; ^P < 0.05 versus corresponding “mock transfected counterpart”. #P < 0.05 versus corresponding “glucose 100 mM counterparts”