Two more people have been charged in connection with the female genital mutilation (FGM) of two seven-year-olds in the first ever federal case in the US.

The defendants are part of a religious and cultural community called Dawoodi Bohra, an Islamic sect based in India, which is alleged to practise FGM.

The procedure has been condemned by the United Nations and is banned in the US.

Detroit Physician Jumana Nagarwala, 44, has been accused of cutting two Minnesota girls, leaving them with scars and lacerations.

Ms Nagarwala is in jail awaiting trial after a federal judge deemed her a flight risk and threat to the community.

Two more people, Dr Fakhruddin Attar, 53, and his wife, Farida Attar, 50, were arrested at their medical office in Livonia, west of Detroit, Michigan, where the alleged procedures were performed in February.

They were charged with conspiracy, female genital mutilation and aiding and abetting.

Investigators saw the two girls and their mothers arrive at the Burhani Medical Clinic with Dr Attar, his wife and Ms Nagarwala already inside, CNN reported.

According to court documents, the girls said they were told they were going to Detroit for “a special girls' trip”. One of the girls said that after the procedure “she could barely walk, and that she felt pain all the way down to her ankle”.

The girls said their parents told them not to talk about what happened and one couple described it as a "cleansing" of extra skin.

Dr Attar told investigators Ms Nagarwala saw patients in his clinic five to six times a year and said he believed he had done nothing to violate the law.

Ms Nagarwala’s attorney, Shannon Smith, said the procedure did not involve mutilation but was a benign religious ritual.

She also said the clinic was used to keep the procedure sterile, CNN reported.

According to The Detroit News, members of the Dawoodi Bohra group in the area belong to the Anjuman-e-Najmi mosque, the only Dawoodi Bohra mosque in the area.

Leaders of the mosque released a statement saying they were offering assistance to investigators.

“Any violation of US law is counter to instructions to our community members. It does not reflect the everyday lives of the Dawoodi Bohras in America," the statement said.

“It is an important rule of the Dawoodi Bohras that we respect the laws of the land, wherever we live. This is precisely what we have done for several generations in America. We remind our members regularly of their obligations.”

No charges have been filed against the parents of the girls.

FGM tribal circumcision ceremony in Baringo County Show all 12 1 /12 FGM tribal circumcision ceremony in Baringo County FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot girl cries after being circumcised REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM The traditional practice of circumcision within the Pokot tribe is a rite of passage that marks the transition to womanhood and is a requirement for all girls before they marry Reuters FGM tribal circumcision ceremony in Baringo County Kenya FGM Pokot girls are encouraged to leave their hut and make their way to a place where they will take off their clothes and wash during their circumcision ceremony REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot girl, covered in animal skins, walks to a place where she will rest after being circumcised in a tribal ritual in a village about 80 kilometres from the town of Marigat in Baringo County REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM Pokot girls, draped in animal skins, sit on rocks during their circumcision ceremony REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot woman performs a circumcision on a girl REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot woman holds a razor blade after performing a circumcision on four girls REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot girl bleeds onto a rock after being circumcised REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot girl is smeared with a white paint after being circumcised REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM More than a quarter of girls and women in Kenya have undergone genital cutting, according to United Nations data REUTERS FGM tribal circumcision ceremony in Baringo County Kenya FGM A Pokot girl, covered in animal skins, walks to a place where she will rest after being circumcised Reuters FGM tribal circumcision ceremony in Baringo County Kenya FGM Pokot girls covered with animal skins squat on rocks after being stripped naked and washed during their circumcision rite in a village about 80 kilometres from the town of Marigat in Baringo County REUTERS

There are no medical, health or hygienic reasons for FGM. Instead, it is linked to beliefs about honour, women’s sexuality and marriage and is seen as a rite of passage into adulthood.

In 2016, Unicef estimated 200 million women in 30 countries had undergone the painful procedure.

Critics say FGM can cause complications during childbirth, make intercourse painful and eliminate any pleasure a woman can derive from sex.