Media playback is unsupported on your device Media caption The plastic particles lurking in your bottled water

Tests on major brands of bottled water have found that nearly all of them contained tiny particles of plastic.

In the largest investigation of its kind, 250 bottles bought in nine different countries were examined.

Research led by journalism organisation Orb Media discovered an average of 10 plastic particles per litre, each larger than the width of a human hair.

Companies whose brands were tested told the BBC that their bottling plants were operated to the highest standards.

The tests were conducted at the State University of New York in Fredonia.

Sherri Mason, a professor of chemistry at the university, conducted the analysis and told BBC News: "We found [plastic] in bottle after bottle and brand after brand.

"It's not about pointing fingers at particular brands; it's really showing that this is everywhere, that plastic has become such a pervasive material in our society, and it’s pervading water - all of these products that we consume at a very basic level."

Currently, there is no evidence that ingesting very small pieces of plastic (microplastics) can cause harm, but understanding the potential implications is an active area of science.

Image copyright Orb Media Image caption After filtration, the larger particles - yellow marks - are easy to see

Commenting on the results, Prof Mason said: "It's not catastrophic, the numbers that we're seeing, but it is concerning."

Experts have told the BBC that people in developing countries where tap water may be polluted should continue to drink water from plastic bottles.

Contacted to comment on the findings, the companies behind the brands have insisted that their products meet the highest standards for safety and quality.

They also point to the absence of any regulations on microplastics and of the lack of standardised methods of testing for them.

Last year, Prof Mason found plastic particles in samples of tap water and other researchers have spotted them in seafood, beer, sea salt and even the air.

This latest work comes amid growing international attention on plastic, fuelled by the BBC's acclaimed Blue Planet 2 series in which Sir David Attenborough highlighted the threat of plastic waste in our oceans.

The research into bottled water involved buying packs from 11 different global and national brands in countries chosen for their large populations or their relatively high consumption of bottled water. These were:

Leading international brands:

Aquafina

Dasani

Evian

Nestle Pure Life

San Pellegrino

Leading national brands included:

Aqua (Indonesia)

Bisleri (India)

Epura (Mexico)

Gerolsteiner (Germany)

Minalba (Brazil)

Wahaha (China)

Image caption A dye is used that binds to pieces of plastic

To eliminate any risk of contamination, purchases in shops and deliveries to courier companies were recorded on video. Some packs in the US were ordered over the internet.

The screening for plastic involved adding a dye called Nile Red to each bottle, a technique recently developed by British scientists for the rapid detection of plastic in seawater.

Previous studies have established how the dye sticks to free-floating pieces of plastic and makes them fluoresce under certain wavelengths of light.

Prof Mason and her colleagues filtered their dyed samples and then counted every piece larger than 100 microns – roughly the diameter of a human hair.

Some of these particles – large enough to be handled individually - were then analysed by infrared spectroscopy, confirmed as plastic and further identified as particular types of polymer.

Particles smaller than 100 microns – and down to a size of 6.5 microns – were much more numerous (an average of 314 per litre) and were counted using a technique developed in astronomy for totalling the number of stars in the night sky.

The make-up of these particles was not confirmed but Prof Mason said they can "rationally expected to be plastic".

This is because although Nile Red dye can bind to substances other than plastic - such as fragments of shell or algae containing lipids - these would be unlikely to be present in bottled water.

Since the study has not been through the usual process of peer review and publication in a scientific journal, the BBC has asked experts in the field to comment.

Dr Andrew Mayes, of the University of East Anglia and one of the pioneers of the Nile Red technique, told us it was "very high quality analytical chemistry" and that the results were "quite conservative".

Michael Walker, a consultant to the Office of the UK Government Chemist and founder board member of the Food Standards Agency, said the work was "well conducted" and that the use of Nile Red has "a very good pedigree".

Both of them emphasised that the particles below 100 microns had not been identified as plastic but said that since the alternatives would not be expected in bottled water, they could be described as "probably plastic".

One obvious question is where this plastic may be coming from. Given the amount of polypropylene, which is used in bottle caps, one theory is that the act of opening a bottle may shed particles inside.

To check that the process of testing was not itself adding plastic to the bottles, Prof Mason ran "blanks" in which the purified water used to clean the glassware and the acetone used to dilute the Nile Red dye were themselves investigated.

Small quantities of plastic were found in them – believed to be from the air - but these were subtracted from the final results.

A surprise to researchers was the wide variety of findings – 17 of the 259 bottles tested showed no evidence of plastic but all of the rest did, with big differences even within brands.

A few bottles were found to have thousands of particles - the vast majority being the smaller ones that are "probably plastic" - but others from the same pack had virtually none.

Image caption Prof Mason takes David Shukman through the results of the study

We contacted the companies involved and most responded.

Nestle told us its own internal testing for microplastics began more than two years ago and had not detected any "above trace level". A spokesman added that Prof Mason’s study missed key steps to avoid "false positives" but he invited Orb Media to compare methods.

Gerolsteiner also said it had been testing its water for microplastics for a number of years and that the results showed levels "significantly below the limits for particles" set for pharmaceutical companies. It said it could not understand how Prof Mason’s study reached its conclusions.

It also said its measures exceeded industry standards but added that microparticles are "everywhere" so "the possibility of them entering the product from ambient air or packaging materials during the bottling process can therefore not be completely ruled out".

Coca-Cola said it had some of the most stringent quality standards in the industry and used a "multi-step filtration process". But it too acknowledged that microplastics "appear to be ubiquitous and therefore may be found at minute levels even in highly treated products".

Danone said it could not comment on the study because "the methodology used is unclear" but added that its own bottles had "food grade packaging".

It pointed out that there are no regulations on microplastics or a scientific consensus on how to test for them, and it also highlighted a much smaller German study last year that found plastic particles in single use bottles but not above a statistically significant amount.

PepsiCo said Aquafina had "rigorous quality control measures sanitary manufacturing practices, filtration and other food safety mechanisms which yield a reliably safe product".

It described the science of microplastics as "an emerging field, in its infancy, which requires further scientific analysis, peer-reviewed research and greater collaboration across many stakeholders".

The full Orb Media report can be found at www.OrbMedia.org

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