Bacteria free clean air is important for healthy life. Current research is mainly focused on nonwoven and antibacterial breath mask development. Copper oxide was selected as antibacterial agent due to its good antimicrobial properties as well as its production is economical as compared to other metallic nanoparticles which are being used as antibacterial agents i.e. Copper nanoparticles, gold nanoparticles, and silver nanoparticles. Copper (II) oxide is hydrophilic in nature and is one of the two stable oxides of copper. Prepared nanofibers were characterized for possible testing which include morphological characterization, surface area and porosity measurement using BET, mechanical properties using Universal Testing Machine, elemental analysis using XPS, crystallinity assessment by XRD, breathability test by upright cup method, air permeability test, thermal analysis by TGA, and antimicrobial properties by disk diffusion method. Drug release properties were assessed by ICP. Presence of copper oxide nanoparticles exhibited excellent morphological, mechanical, structural, surface, and antimicrobial properties (samples having 1.00% CuO exhibited optimum results).